1.Application of arsenic trioxide in gastric cancer treatment
Journal of International Oncology 2013;(6):460-462
Arsenic trioxide (As2O3)is the main component of traditional Chinese medicine arsenic,which was initially used in the treatment of blood system diseases.In recent years,studies have found that it also has a good effect on a variety of solid tumors,especially gastric cancer.It has been confirmed that As2O3 can inhibit proliferation and metastasis of gastric cancer cells,induce apoptosis and protective autophagy,inhibite lymphatic formation and angiogenesis,affect the expression of telomere-related proteins in gastric cancer cells.
2.Protective effects of allopregnanolone against different seizure models in mice
Chinese Journal of Clinical Pharmacology and Therapeutics 2000;5(2):97-100
Aim To examine the protective effects of allopregnanolone against seizure on different animal models.Methods The protective effects of allopregnanolone against maximal electrical seizure (MES) and picrotoxin-induced seizure were studied in C57 mice 15 minutes after vehicle or drug intraperitoneal administration.Results In the MES test, we found that pretreatment with the phenobarbital or allopregnanolone produced a dose-dependent protective effect against seizure. The potency (ED50 value) of phenobarbital in the MES test was 2.40 mg·kg-1, with 95% confidence interval range from 1.22 to 4.72 mg·kg-1. The potency (ED50 value) of allopregnanolone in the MES test was 0.086 mg·kg-1, with 95%confidence interval range from 0.037 to 0.201 mg·kg-1, which was significantly higher than that of phenobarbital (P<0.01). The combination study of half ED50 values of phenobarbital and allopregnanolone resulted in a 80% of protective effect in MES test, which was higher than 50% produced by either phenobarbital or allopregnanolone at their ED50 values respectively. This result indicated that there was a synergism between phenobarbital and allopregnanolone in their anticonvulsant activities. In the picrotoxin test, we found that pretreatment with the allopregnanolone also produced a dose-dependent protective effect against picrotoxin-induced seizure. The potency of allopregnanolone in the picrotoxin seizure test was 0.123 mg·kg-1, with 95% confidence interval range from 0.058 to 0.263 mg·kg-1.Conclusion Allopregnanolone(ip) could protect different seizures in a dose-dependent manner,had a higher potency than phenobarbital,and had synergism with phenobarbital in the MES test.
3.Effect of therapeutic communication on perioperative anxiety of cancer patients: a Meta-analysis
Chinese Journal of Practical Nursing 2015;31(13):997-1001
Objective To evaluate the effect of therapeutic communication on perioperative anxiety of cancer patients by using the Meta-analysis.Methods The randomized controlled studies of therapeutic communication for perioperative anxiety of cancer patients were gathered from Pubmed,Embase,Cochrane Library,China Biology Medicine Disc (CBM),China Scientific Journal Database by VIP (CSJD),China National Knowledge Infrastructure (CNKI) and Wangfang Data,while other relative researches were also searched by hand.According to inclusion and exclusion criteria articles were evaluated by two reviewers independently.The studies were assessed according to the Juni assessment.RevMan 5.2 was used for statistical analysis.Results Seven studies with 506 patients were included,of which therapeutic intervention group contained 256 patients,the control group included 250 patients.Meta-analysis showed that compared with the control group,therapeutic communication system could reduce perioperative anxiety of cancer patients [MD=-8.00,95%CI (-8.91--7.10),P <0.01],improve positive perioperative coping of patients [MD=5.50,95%CI(4.05-6.95),P < 0.01],reduce negative perioperative coping of patients [MD=-7.62,95%CI (-9.13--6.11),P < 0.01],increase the satisfaction degree of patients [MD=0.55,95%CI (0.33-0.77),P <0.01],the differenccs were statistically significant.Conclusions Therapeutic communication can contribute to relieve perioperative anxiety of cancer patients,optimize their coping styles and improve the satisfaction degree of patients with nursing work.
4.Investigation on the running condition of defluoridation projects in Anyang City of Henan Province in 2006
Chinese Journal of Endemiology 2008;27(5):524-525
Objective To investigate the running condition of defluoridation projects and fluoride concentration in drinking-water in Anyang City of Henan Province.Methods Cluster sampfing of Anyang,Hua, Neihuang,Tangyin Counties emdimic fluomsis poining of Anyang City was conducted places of different orientation, about 1/3 towns in the selected examination towns had their defluoridation project investigated.Results Among 472 pwjects investigated,427 were running normally,accounting for 90.47%(427/472),only 9.53%(45/472)of the projects were out of service.68.62%(293/427)of the pwjects had normal fluoride concentration in drinking water, 31.38%(134/427)of the projects failed to do 80.In Hua County,41.00%(41/100)of the projects were out of service. In Neihuang County.70.39%(107/152)of the projects had abnormally high fluoride concentration in drinking water. Conclusions Defluoriding projects are out of service and fluoride concentration in drinking water exceeds the standard in Hua,Neihuang Counties of Anyang City.We must analyse the cause of it seriously,work out measures, promote a healthy and sustainable development of defluondation projects
5.Regulation of non-immunomodulatory interaction between pertussis toxin and host cells
International Journal of Pediatrics 2021;48(5):331-334
Pertussis toxin(PTx), an important toxin that produced by Bordetella pertussis, is an important pathogenic factor in the pathogenesis of whooping cough.The previous studies have showed that PTx interacting proteins vary distinctly in size, ranging from the 43 kD surface protein of human lymphocyte to the 200 kD surface protein of insulin secreting cells.PTx has been shown to interact with N-linked oligosaccharides on the surface of host cells, sialoglycoprotein-like factors, glycoproteins such as haptoglobin, fetuin, G D1a glycolipids.Especially, the interaction of PTx with its receptor Gi protein can result in increased intracellular cAMP level, which will lead to multiple physiological responses.However, the current limited understanding of other PTx modified substrates and interacting proteins in cells has seriously hindered our understanding on the mechanism that PTx regulates other signaling pathways and physiological functions of the host.
6.Data quality in clinical trials: the role of blind review.
Acta Pharmaceutica Sinica 2015;50(11):1498-501
Blind review is one of the most important milestones in clinical trials, which connects data management process to statistical analysis. During blind review, data quality should be reviewed and assessed on both data management and statistical aspects. The primary work of data managers in blind review is to ensure the accuracy of data before it is handed over to biostatistics group. Database auditing, listing data reviewing and reconciliation should become a good clinical data management practice. Statisticians, on the other hand, will focus on quality findings related to protocol deviations or protocol violations. To investigate the protocol deviations and/or violations and relevant impacts on data outcomes, it is important to provide the essential basis of data quality through the blind review, and to assess the reliability of study outcomes.
7.Effect of minocycline hydrochloride ointment combined with ibuprofen on serum concentration in chronic periodontitis
Chinese Journal of Biochemical Pharmaceutics 2017;37(3):184-186
Objective To study the effect of minocycline hydrochloride ointment and ibuprofen on serum concentration of chronic periodontitis . Methods 86 patients with chronic periodontitis treated in our hospital from June 2014 to June 2016 were selected and randomly divided into two groups:observation group 43 cases, using minocycline hydrochloride ointment combined with ibuprofen treatment , the control group 43 cases, only the use of minocycline hydrochloride ointment for treatment.After treatment, the clinical efficacy of the two groups were observed, the level of plaque, the level of attachment, the level of gingival sulcus bleeding and the probing depth of periodontal pocket were recorded, the levels of IL-10, TNF-α, hs-CRP and IL-8 in serum and the incidence of adverse reactions were measured.Results The total effective rate was 90.7% in the observation group and 74.4%in the control group, which was significantly higher than that in the control group, the difference was statistically significant (P<0.05).The probing depth of the periodontal pocket, gingival sulcus bleeding index, attachment level and plaque index decreased to varying degrees in the two groups before and after treatment, the difference was statistically significant (P<0.05), patients in the observation group were significantly lower than those in the control group, the difference was statistically significant (P<0.05).After treatment, the levels of IL-8, hs-CRP and TNF-αin serum of the two groups decreased, IL-10 levels increased in different degrees, the difference was statistically significant (P<0.05).The observation group was better than the control group, the difference was statistically significant ( P<0.05 ) .Conclusion Minocycline hydrochloride ointment combined with ibuprofen has a good effect on patients with chronic periodontitis.It can effectively relieve the patient's condition and improve the serum concentration of patients.
8.Effect of antioxidants on secondary conformational transition of aged amyloid ?-peptide_( 1-40) by FT-IR quantitative study
Chinese Journal of Pathophysiology 1989;0(06):-
AIM: To investigate the mechanism that antioxidants TA9901, inhibit the formation of amyloid-?-protein(A?) fibril. METHODS: Fourier-transform infrared spectroscopy was used to study the secondary structure changes on aging A? in vitro. RESULTS: A? aged alone for 30 min, the content of ?-pleated sheet and ?-turn were 43.17% and 32.9% respectively. A? aged alone for 7 days, the content of ?-pleated sheet increased abuot 10% and produced a shift of random coil toward ?-pleated sheet. TA9901 induced a significant decrease of the content of ?-turn (23.5%) and ?-pleated sheet (26.4%). VE mainly decreased the ?-pleated sheet content (30.8%). The combination of TA9901 and VE promoted transition of ?-turn (16.7%) toward ?-helix and random coil. CONCLUSIONS: Both of TA9901 and VE can effectively diminish the ?-structural content. TA9901 showed more intensitive inhibition than VE. The effect of TA9901 on the secondary structure of aged A? was associated with the mechanism that TA9901 inhibited A? aggregation and fibril formation.
9.Effect of Hedgehog pathway on myocardial infarction and hypoxic-ischemic cardiomyocytes
Chinese Pharmacological Bulletin 2016;32(5):676-680,681
Aim To investigate the effect of Sonic Hedgehog on normal hearts and hypoxic-ischemic myo-cardial cells. Methods A method for left anterior de-scending artery ( LAD ) ligation was employed to con-struct the myocardial infarction model, and ultrasonic cardiogram was used for identification. Western blot and immunofluorescence staining were used to detect expressions of Shh, Ptch-1, Smo and Gli-1 in H9C2 cells and H2 O2-induced H9C2 cells, and that in 12 ca-ses of myocardial infarction tissues and 9 cases of nor-mal myocardium, respectively. Agonist and antagonist of Shh pathway were adminstered in the hypoxic-ische-mic myocardial H2 O2-induced H9C2 cell model, and once again expressions and strength of Shh, Ptch-1, Smo, Gli-1 were detected. Results Shh and Gli-1 were not expressed in normal hearts, but expressed in hearts with myocardial infarction;Ptch-1 and Smo were expressed in both normal hearts and hearts with myo-cardial infarction. Under the action of agonist, expres-sions of Shh and Gli-1 increased in the hypoxic-ische-mic H9C2 cell model. Similarly, Shh and Gli-1 were not expressed in normal H9C2 cells, but in H2 O2-in-duced H9C2 cells, and Ptch-1 and Smo were expressed in both normal H9C2 and in H2 O2-induced H9C2 cells. Conclusion Shh signaling pathway can be acti-vated in the condition of ischemia and oxidative stress, and then it promotes the repairing of myocardial cell damage.
10.Clinical evaluation of patroline adjuvant therapy for retrograde pulpitis
Chinese Journal of Biochemical Pharmaceutics 2017;37(7):321-322,324
Objective To improve the traditional treatment of retrograde pulpitis, using Periocline adjuvant therapy, to evaluate its clinical effect.Methods From January 2015 to 2017 in January, our hospital 70 patients treated with retrograde pulpitis.The patients were randomly divided into two groups.The observation group of 35 cases, adopt the traditional treatment methods (periodontal treatment and dental pulp treatment), 35 cases in the control group based on conventional therapy, the adjuvant treatment of periocline.The related indexes and treatment effects were observed.Results After treatment, the indexes of the two groups of patients were significantly decreased compared with the control group[(1.57±0.50),(3.37±1.88),(4.89±1.60),(5.31±1.39)],the observation group decreased much more[(0.79±0.24),(2.01±1.14),(4.01±1.38),(4.11±0.62)], the difference was significant (P<0.05).At the same time, the effective rate of the observation group was 94.29% (33 cases), and the effective rate of the control group was 74.29% (26 cases), the difference was significant (P<0.05).Conclusion Based on conventional therapy, using Periocline adjuvant therapy, can improve the therapeutic effect of retrograde pulpitis, has significant value of application and promotion.