Angiotensin-converting enzyme 2 (ACE2)-angiotensin (1-7) [Ang (1-7)]-Mas constitutes the vasoprotective axis and is demon-strated to antagonize the vascular pathophysiological effects of the classical renin -angiotensin system .We hypothesize that upregulation of ACE2-Ang (1-7) signaling protects endothelial function through reducing oxidative stress , thus resulting in beneficial outcome in di-abetes.Ex vivo treatment with Ang (1-7) augmented endothelium-dependent relaxation (EDR) in renal arteries from diabetic patients . Both Ang (1-7) infusion via osmotic pump (500 ng? kg -1? min-1 ) for 2 weeks and exogenous ACE 2 overexpression mediated by ad-enoviral ACE2 via tail vein injection rescued the impaired EDR and flow-mediated dilatation ( FMD) in db/db mice.Diminazene acetu-rate treatment (15 mg? kg-1? d-1 ) activated ACE2, increased the circulating Ang (1-7) level, and augmented EDR and FMD in db/db mouse arteries.In addition, activation of the ACE2-Ang (1-7) axis reduced reactive oxygen species (ROS) overproduction de-termined by dihydroethidium staining , CM-H2DCFDA fluorescence imaging , and chemiluminescence assay in db/db mouse aortas and also in high-glucose-treated endothelial cells .Pharmacological benefits of ACE 2-Ang ( 1-7 ) upregulation on endothelial function were confirmed in ACE2 knockout mice both ex vivo and in vitro.We elucidate that the ACE2-Ang (1-7)-Mas axis serves as an important signal pathway in endothelial cell protection in diabetic mice , especially in diabetic human arteries .In summary, endogenous ACE2-Ang (1-7) activation or ACE2 overexpression preserves endothelial function in diabetic mice through increasing nitric oxide bioavail -ability and inhibiting oxidative stress , suggesting the therapeutic potential of ACE 2-Ang(1-7) axis activation against diabetic vasculop-athy.

Relations between doctor and patient are basic and important human relation in medical practice.Because of some reasons,the relations appear tense status in our country,moreover the market economy flourishingly develops,as the result of medical organizations suffering more and more existent pressure.It is an important subject faced for people,especially for medical worker,to righly think the existed defects,and set up the mutual action between doctor and patient.

Extranodal natural killer (NK)/T-cell lymphoma, nasal type (ENKTL) is an aggressive non-Hodgkin lymphoma and there are no standard treatment options for ENKTL. For localized patients, radiotherapy alone or radiotherapy combined with chemotherapy could be took into consideration. Systemic combination chemotherapy remains the primary treatment for patients with advanced ENKTL. The efficacy of anthracycline based conventional chemotherapy in ENKTL is unfavorable. This paper reviews the treatment progress of ENKTL in recent years.

Endothelial Cells ; metabolism ; physiology ; Stem Cells ; metabolism ; physiology

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Heart Failure ; therapy ; Humans

Objective To investigate the effects of compound Danshen injection on stress-induced gastric mucosal damage and its mechanism.Methods Sprague Dawley rats were used to establish immersion restraint cold stress model.50 SD rats were randomly divided into five groups(n=10 per group):① the normal control group; ② the stress model group; ③ the group treated with low dose of compound Danshen injection(50 mg/kg) ; ④ the group treated with medial dose of compound Danshen injection(100 mg/kg) ; ⑤the group treated with high dose of compound Danshen injection (200 mg/kg).The gastric mucosal ulcer index (UI),the level of malondialdehyde (MDA) in gastric mucosa,the activity of superoxide dismutase (SOD),and plasma intedeukin-1beta (IL-1β)content were detected.Results Compared with the normal control group [(0.7± 0.4) mm,(0.25 ± 0.03)nmol/mg,and (246.5 ± 45.4)Nu/mg],the stress model group exhibited a markedly increase in gastric mucosal UI[(28.4±4.5)mm](P＜0.01) and MDA level [(0.60±0.08)nmol/mg](P＜0.01) and a decrease in the activity of gastric mucosal SOD [(122.5 ±14.2) Nu/mg] (P＜0.01).In addition,an elevated level of plasma IL-1β[(31.6±8.4) pg/ml vs (8.5±3.1) pg/ml] (P＜0.01) was observed in the stress model group.Pretreatment with compound Danshen injection dose-dependently attenuated the gastric mucosal damage,characterized by a markedly decrease in gastric mucosal UI [(22.8 ± 3.7)mm、(12.2 ±3.5)mm,(6.2 ± 1.6)mm] and MDA level [(0.52 ± 0.07)nmol/mg,(0.32 ± 0.06)nmol/mg,(0.28 ±0.03)nmol/mg] and an increase in the activity of gastric mucosal SOD[(135.2± 13.6)Nu/mg,(220.7±33.5)Nu/mg,(251.2±23.7)Nu/mg] (P＜0.01).Meanwhile,compound Danshen injection reduced the content of plasma IL-1β[(27.2±7.5)pg/ml,(13.5±5.3)pg/ml,(9.3±4.4)pg/ml] (P＜0.01).Conclusion Compound Danshen injection exhibits preventive protection on the stress-induced gastric mucosal damage by water immersion restraint cold stress in SD rats,the mechanism of which might be linked to its inhibition of gastric mucosal oxidation stress and the reduced release of inflammatory mediator IL-1β.

Objective To investigate the risk factors of post-pancreaticoduodenectomy complications.Methods The clinical data of 207 patients with pancreatic carcinoma or peri-ampullary carcinoma who underwent pancreaticoduodenectomy at the Anhui Provincial Hospital from Dec.2007 to Dec.2012 were studied.Seventeen clinicopathologic factors to correlate with the postoperative mortality and methods were selected for univariate analysis and multivariate analysis using logistic regression.Results Univariate analysis showed the major risk factors of postoperative mortality and morbidity were a history of previous operation,pre-operative drainage,total serum bilirubin level,alanine aminotransferase level,serum albumin level,serum pre-albumin level,parenchyma texture and pancreatic duct diameter (P＜0.05).Multivariate analysis showed alanine aminotransferase level,parenchyma texture and pancreatic duct diameter were independent risk factors of postoperative complications.Pancreatic duct diameter was the independent risk factor of pancreatic fistula.Pancreatic fistula was the independent risk factor of hemorrhage.Conclusion The postoperative complications of pancreaticoduodenectomy was closely related to alanine aminotransferase level,parenchyma texture and pancreatic duct diameter.

Gliomas harbor a small population of cells termed glioma stem-like cells (GSC),which have the ability to undergo self-renewal and initiate tumorigenesis.GSCs are resistant to a wide variety of chemotherapeutic agents and possess a remarkable ability to recover from cytotoxic therapy.Furthermore,GSCs play a crucial role in RT failure,as tumors surviving RT are enriched in GSCs.Therefore,an alternative strategy involving selective targeting of this functionally distinct chemo-and radiation resistant small group of GSCs rather than the bulk of the tumor may be more successful in treating this deadly disease.GSC exhibit various alterations to signaling pathway activity,which are associated with self-renewal and neoplastic proliferation.These altered pathways may represent possible targets for GSC.

Relations between doctor and patient are basic and important human relation in medical practice.Because of some reasons,the relations appear tense status in our country,moreover the market economy flourishingly develops,as the result of medical organizations suffering more and more existent pressure.It is an important subject faced for people,especially for medical worker,to righly think the existed defects,and set up the mutual action between doctor and patient.

Objective To investigate the relationship between sarcoplasmic reticulum Ca2+modulation proteins and postresuscitation myocardial dysfunction. Methods Thirty-eight SPF male Sprague-Dawley (SD) rats were randomly divided into control group(n=12)and cardiac arrest(CA)group(n=26). CA was induced by intravenous bolus of potassium chloride(40μg/g),and cardiopulmonary resuscitation(CPR)was conducted 8 minutes later. No CA was induced in control group except catheter placement for monitoring cardiopulmonary parameters after anesthesia. Invasive hemodynamic parameters were monitored for 1 hour after CPR. Echocardiogram was performed to evaluate cardiac function. Myocardial samples were harvested 5 minutes and 1 hour after restoration of spontaneous circulation (ROSC),and sarcoplasmic reticulum Ca2+ ATPase (SERCA2a),phosphorylated phospholamban (p-PLB) and rynodine receptor(RyR)were determined by Western Blot. Results ROSC rate of CA group was 92.3%(24/26),and mean recovery time was (68 ±39)seconds. Cardiac function was significantly impaired in CA group at 1 hour after resuscitation, and ejection fraction, fraction shortening (FS), the maximal rate of left ventricular pressure increase/decline (±dp/dt max)were significantly decreased compared with those in control group 〔ejection fraction:0.548±0.060 vs. 0.809±0.043,F=71.692,P=0.000;FS:(34.4±4.4)%vs. (46.0±3.5)%,F=55.443,P=0.000;+dp/dt max(mmHg/s):4 718±743 vs. 7 098±394,P<0.01;-dp/dt max(mmHg/s):-3 824±612 vs.-6 187±473,P<0.01〕. Compared with control group,the expression levels of p-PLB (gray value)was significantly decreased at 5 minutes and 60 minutes(5 minutes:0.64±0.15 vs. 1.29±0.13,P<0.01;60 minutes:0.95±0.08 vs. 1.30±0.09,P<0.05)after resuscitation in CA group,while the level of sarcoplasmic SERCA2a(gray value)and RyR (gray value)showed no significant differences(SERCA2a 5 minutes:1.01±0.18 vs. 1.24±0.07,60 minutes:1.03± 0.14 vs. 1.25 ±0.06;RyR 5 minutes:0.96 ±0.13 vs. 0.97 ±0.13,60 minutes:0.88 ±0.14 vs. 0.99 ±0.11,all P>0.05). Conclusions The impairment of the p-PLB is closely related to postresuscitation myocardial dysfunction.