Objective To investigate the effects of intravenous injection of α-crystallin on retinal ganglion cells (RGC) and some important organs of the Long Evans rats. Methods RGC were retrogradelabeled by fluorogold through bilateral superior colliculus and lateral geniculate body for seven days before optic nerve crush injury. Twenty-three Long Evans rats were used for this study, including three rats of normal control group and 20 rats of experimental group. Twenty rats were randomly divided into saline control group and three α-crystallin injection groups, which received tail vein injection of 1.25 ml isotonic saline and three different concentrations (1 × 10-2 , 1 × 10-1 and 1 g/L) of α-crystallin respectively, once every two days and totally seven times. After two weeks, the labeled RGC were counted, and the pathological changes on liver, kidney, brain, spleen and the lungs were investigated. Results Compared with the normal control group, although the number of RGC markedly decreased after two weeks of optic nerve crush injury in every group, the number of RGC in α-crystallin-treated groups was more than those in the saline control group. There were 2074± 150 RGC per mm2 in normal control group, 85 ± 15 RGC per mm2 in saline control group, 124±26 RGC per mm2 in 1 × 10-2 g/L α-crystallin group, 128± 31 RGC per mm2 in 1 × 10-1 g/L α-crystallin group, 164 ± 20 RGC per mm2 in 1 g/L α-crystallin group (F= 18. 660,P＜0. 01). No congestion, swelling, inflammation and other pathological changes were found in liver,kidney, brain, spleen and lung. Conclusions Intravenous injection of α-crystallin protein has protective effects on RGC after the optic nerve crush injury, and no significant effects on important organs.

Amniotic Fluid ; cytology ; Cardiomyopathies ; therapy ; Humans ; Stem Cells ; cytology

Purpose: To report 2 cases of papillary thyroid carcinoma occurring in a thyroglossal duct remnant, and to discuss the diagnostic and therapeutic methods by reviewing the literature. Methods: An asymptomatic midline mass occurring in the upper neck was the sole presenting complaint in 2 cases. The preoperative evaluation included a complete head and neck examination, B-ultra sound examination and FNAB. The Sistrunk procedure was done. A lobectomy( case 1) and a lumpectomy ( case 2) were performed respectively, because of the abnormality found in their thyroid gland. A modified neck dissection was performed in case 2 because of regional lymphadenopathy. The following histologic studies were carried out on tissues with HE stain. Results: Diagnoses of thyroglossal duct carcinoma were made by several pathologists. Tissues from thyroid were first diagnosed as nodular goiter in case 1 and thyroid adenoma in case 2. In case 2, one positive lymph node and invasion to the hyoid bone was found. Conclusions: Malignant lesions are rare in the thyroglossal duct remnant. The diagnostic criteria is acknowledged. Resection of the thyroglossal duct carcinoma by the Sistrunk procedure is an adequate surgical approach. But the controversy about further treatment will continue because of the lack of large series of patients and the 10 to 20 year follow-up.

Chromatography ; methods ; DNA Fingerprinting ; Drugs, Chinese Herbal ; analysis ; Magnetic Resonance Spectroscopy ; Mass Spectrometry

Objective To assess the disease severity and prognosis value by observing the kinetic change of serum procalcitonin(PCT)and PCT clearance rate(PCTc)in the patients with ventilator associated pneumonia (VAP). Methods A single-center prospective observational study was conducted. A total of 128 patients with VAP admitted into intensive care unit(ICU)of First Affiliated Hospital of Xinjiang Medical University from February 2012 to June 2014 were enrolled. The patients were divided into recovery group(n=88)and deterioration group(n=40) according to the therapeutic outcome. The acute physiology and chronic health evaluationⅡ(APACHEⅡ)scores were estimated within 24 hours when VAP was diagnosed. The serum PCT(PCT1,PCT5,PCT7,PCT9)and PCTc(PCTc5, PCTc7,PCTc9)were examined at 1,5,7 and 9 days after the VAP was diagnosed. The diagnostic and predictive performance of PCT,PCTc and APACHEⅡ scores were assessed by the receiver operating characteristic curve (ROC). Results APACHEⅡscores in recovery group were significantly lower than those in the deterioration group (14.49±5.30 vs. 18.90±5.30,t=-4.349,P=0.000). There was no significant difference in PCT level(μg/L)at 1 day after VAP was diagnosed between recovery group and deterioration group〔2.84(0.81,6.43)vs. 3.50(0.97,10.27), Z=-1.431,P=0.152〕. With prolonged treatment,PCT was gradually decreased in recovery group,while remained at higher level in deterioration group,which was significantly lowered at 5 days after VAP diagnosed in recovery group compared with that in the deterioration group〔1.28(0.65,3.13)vs. 2.39(0.78,9.35),Z=-2.012,P=0.044〕. PCTc maintained higher level in recovery group which was gradually increased with the improvement of the disease, and PCTc in deterioration group was lowered which was gradually decreased with the development of the disease. PCTc at 5,7,9 days in recovery group was significantly higher than that in deterioration group〔5 d:50.43(20.39,80.60)%vs. -56.68(-286.28,172.92)%, Z=-2.250, P=0.024;7 d:54.01(5.70,102.30)% vs. -76.91(-335.03, 181.21)%,Z=-2.561,P=0.010;9 d:63.88(25.93,101.80)%vs.-133.49(-547.20,280.16)%,Z=-3.133, P=0.002〕. The area under ROC curve(AUC)of PCT5,PCT7,PCT9 predicting the prognosis was 0.591,0.683, 0.746,respectively〔95% confidence interval(95%CI)was 0.456-0.726(P=0.161),0.557-0.808(P=0.005), 0.631-0.860(P=0.000)〕. When PCT9 was 5.65μg/L,the sensitivity of 95%and the specificity of 61%. The AUC of PCTc5,PCTc7 and PCTc9 was 0.648,0.685,0.729,respectively〔95%CI was 0.513-0.783(P=0.028),0.555-0.815(P=0.006),0.607-0.851(P=0.001)〕. When PCTc9 was 92%,the sensitivity was 98%and the specificity was 71%. The AUC of APACHEⅡscore was 0.693(95%CI 0.578-0.808,P=0.003). When APACHEⅡscore was 19.5,the sensitivity was 77%and the specificity was 58%. Conclusions The increased levels of PCT in patients with VAP were associated with the poor control of infection and may indicate the deterioration of VAP,it also can reflect the activity of lung infection in time. Keep observing the dynamic change of PCT and analyzing PCTc is more useful. The PCTc levels may provide evidence of disease progression and helpful in risk stratification in patients with VAP,and lower level of PCTc may accompany serious infection and predict poor prognosis.

Objective To promote preventive and therapeutic measures for osteoporosis by investigating the prevalence of low bone mineral density (BMD) in connective tissue disease patients who were taking corticosteroid and identify the associated risk factors of osteoporosis.Methods Thirty-eight female patients who had connective tissue diseases and were taking corticosteroid in PU MCH rheumatology outpatient clinic were studied and followed up from Oct 2006 to April 2007.Lumber spine and right femoral bone mineral density were determined bv dual energy X ray absortiometry (DEXA).Clinical information was obtained from a questionnaire of history and medical records.Duration and accumulative dosage of glucocorticoid intaking.menopause time were obtained retrospectively.Correlation analysis between BMD and clinical information was conducted.Results ① In 38 patients,23 (61%) patients showed a normal BMD,10 (26%) were osteopenia,3 (8%) were iu the osteoporotic range,while 2 patienLs (53%) had fragile fracture.② Compared with patients with normal BMD,subjects with low BMD had significantly older age,longer period after menopause and higher accumulated dose of corticosteroids.③ Postmenopausal women had significantly lower BMD in lumber and hip than premenopausal women.④ Either of vertebral and right hip BMD correlated negatively with the accumulated dosage of cortieosteroids by simple linear regression.⑤ The correlation between BMD and accumulated dosage of corticosteroids improved after correcting for the effect of age by partial correlation analysis (Pearson partial vertebral r=-0.8,P=0.009;right hip r=-0.3,P=0.010).⑥ A stepwise multivariate linear regression model was constructed to explore the relationship between the different clinicsl factors studied and a low BMD.Two statistically significant variables were menopause status (P=0.0000) and a higher steroid accumulated dosage (P=0.008).Conclusion Low BMD is common in connective tissue disease patients receiving corticosteroid.Risk factors for low BMD are postmenopause.duration and the accumulated dosage of glucocorticosteroid.The high prevalence of low BMD implies that more attention should be paid to the prevention and treatment of osteoporosis and fractures in connective tissue disease patients who are taking corticosteroids.

Objective To investigate the effect factors and the relationship of diagnostic system efficiency as soft copy reading with medical LCD.MethodsThirty chest images were selected from PACS on-line.Three of high,mid and low-experienced radiologists interpreted the images on different types of displays independently.Design four display levels based on five factors influencing diagnostic system efficiency,including view distance (0.3 m,0.6 m,0.9 m,1.2 m),monitor resolution ( 1 MP,2 MP,3 MP,5 MP),illuminant level (50 Ix,100 Ix,200 lx,400 lx),view angle (0°,15°,30°,45°) and angle of negative effect light (0°,30°,60°,90°).Three indices of misdiagnosis frequency,diagnosis time and frequency of unable to recognize were analyzed.Orthogonal experimental design and software SPSS 13.0 were used to analyze the results.Results The indices were much different for different factors at different levels.According to the index of average misdiagnosis frequency,extreme difference value of view angle was the lowest (1.4) and angle of negative effect light was the highest (5.0).Extreme difference value of view distance,monitor resolution and illuminant level were 2.9,2.8 and 2.5,respectively.ConclusionsThe order of different factors influencing diagnostic system efficiency is as follows: angle of negative effect light,view distance,monitor resolution,illuminant level and view angle.

Objective　The authors present a retrospective analysis of 22 patients with primary central nervous system lymphoma(PCNSL) in order to provide a reasonable basis for the diagnosis and treatment of the disease. Methods　This report involves a clinicopathological study of 22 patients with histologically proven PCNSL,all diagnosed between January 1993 and May 2000. Univariate and multivariate regression analysis are used to determine prognostic factors significantly associated with an unfavorable or favorable impact on survival. Results　The cohort included 11 men and 11 women whose median age at diagnosis was 49.5 years. At the end point of follow-up, 11 died. The median survival time for the patients in study was 14.5 months. With univariate and multivariate regression analysis, prognostic factors significantly associated with survival included intrathecal injection(P=0.005) and local/diffuse neurological deficit(P=0.031). Conclusion　There continues to be a significantly increasing incidence of PCNSL. This survey throws light on the clinical and prognostic features of this uncommon disease. Through univariate and multivariate regression analysis the authors highly recommend a theraputic regime including surgery, intrathecal injection and chemotherapy, especially those drugs capable of passing blood-brain barrier, for example high dose MTX.

Objective To explore the role of excitatory amino acid carrier 1 (EAAC1)in dorsal root ganglion (DRG) in the mechanism of developing morphine tolerance. Methods Thirty male SD rats were implanted intrathecal catheters and randomized into 6 groups with 5 rats each. The rats of 4 groups were made into the model of adjuvant-induced arthritis in the left hind limb and were administered intrathecally, morphine 10 μg(group M_(10)), morphine 20μg(group M_(20)), morphine 20 μg plus naloxone 10 μg(group MN) ,or saline(group C) respectively. The other 2 groups without were administered intrathecally saline (group C_0) or morphine 20 μg (group M0). The drugs were administered twice daily for 7 days. Mechanical withdrawl threshold(MWT) of the left hind limb was examined to evaluate the behavior. Immunohistochemistry was used to detect the expression of EAAC1 in the left L_(3-4) and L_(4-5) DRG. Results Morphine tolerance was formmed stably in the arthritis rats of group M_(10) and group M_(20) after administering morphine for 7 days. The expression of EAAC1 in DRG was downregulated. Conclusion DRG EAAC1 may be involved in the mechanism of developing morphine tolerance in rats with inflammatory pain.