Objective To investigate the clinical features, BCL-2,CMYC protein expression and prognosis of patients with hepatitis B virus-associated diffuse large B-cell lymphoma (DLBCL).Methods A retrospective study was used to analyse 94 diagnosed DLBCL patients clinical data.Immunohistochemistry method was used to detect the expression of BCL-2 and CMYC protein in paraffin sections of tumor tissues, and to analyze the clinical features, protein expression and prognosis of patients with hepatitis B virus-associated DLBCL.Results ① The rate of HBV infection in the 94 DLBCL patients was 27.66%, significantly higher than the general population(7.18%).Compared with HbsAg-negative DLBCL, HbsAg-positive DLBCL displayed more advanced disease(P=0.032), higher international prognostic index (IPI)(P=0.047) and more frequent involvement of spleen(P=0.02).There were no significant differences in gender distribution, age, immunological subtype and treatment between the two groups.② The aspect of BCL-2 and CMYC protein expression between the two groups: HbsAg-positive group BCL-2 protein expression was significantly higher than HbsAg-negative group (84.6% vs 58.8%, P=0.018).There were no significant differences on the CMYC protein expression and CMYC/ BCL-2 double expression.③ Univariate survival analysis showed that hepatitis B virus infection, BCL-2 positive, CMYC positive, CMYC/ BCL-2 double expression, IPI high-risk group were associated with unfavorable prognostic of overall survival time (OS) and progression free survival (PFS).Cox multivariate analysis showed that CMYC/BCL-2 double expression, IPI high-risk group were independent adverse prognostic factors for OS and PFS.Conclusion HBV infection, BCL-2, CMYC, BCL-2/CMYC double expression, IPI are related to the prognosis of DLBCL.HBV infection may affect the development and prognosis of DLBCL by regulating the expression of BCL-2.

Objective To evaluate the utilization status of essential medicines at community health centers,and analyze problems existing in the stocking and application situation of such medicines,hence to provide references and suggestions for the implementation of the Essential Medicine System.Methods The investigation method was used to analyze the actual stocking situation of essential medicines at community health centers,and existing problems in implementing the Essential Medicine System.Results An average of 377 species of essential medicines were stocked by the community health institutions, accounting for 53.9% of Beijing Essential Medicine List,and average 61.6% of the total medicines in these institutions.There were insufficiencies existing both in the list and supplies of essential medicines. First-aid and low-price essential medicines are out of stock.Lack of confidence by some patients on the drug brands and their quality covered in the essential medicines.Conclusions The list and supplies of essential medicines could not meet the demand of patients at community health centers adequately.The method of evidence-based practice should be applied to each area in implementing of the Essential Medicine System.

Adult ; Cryptococcosis ; physiopathology ; Cryptococcus neoformans ; Duodenal Diseases ; microbiology ; physiopathology ; Humans ; Male

Antineoplastic Agents ; adverse effects ; Humans ; Integrative Medicine ; Organoplatinum Compounds ; adverse effects ; Peripheral Nervous System Diseases ; chemically induced

Objective:To evaluate the in vitro and in vivo immunostimulatory activity and the safety of ethanol extract of wild Artemisia rupestris L. (EEWAR). Methods:Bone marrow dendritic cells (BMDCs) from C57BL/6 mice were treated with different concentrations of EEWAR in vitro and the expression of CD40 and CD80 on BMDCs was detected by flow cytometry. ICR mice were subcutaneously immunized with different concentrations of EEWAR in combination with ovalbumin (OVA) or OVA alone. Aluminum adjuvant was used as the positive control. OVA-specific IgG antibodies in mouse serum samples were measured by ELISA following immunization. T cell proliferation in spleen tissues was detected by MTT method. Acute toxicity test was conducted in ICR mice to analyze the safety of EEWAR. Results:In vitro experiment showed that EEWAR at the concentrations of 10-20 μg/ml increased the expression of CD40 and CD80 on BMDCs ( P<0.05), and had no significant effect on the morphology of BMDCs; EEWAR at the concentrations of 100-200 μg/ml significantly promoted the expression of CD40 and CD80 on BMDCs ( P<0.01), but had a certain influence on the morphology of BMDCs. In vivo experiment showed that EEWAR enhanced the production of IgG, IgG 1 and IgG 2a antibodies against OVA and the proliferation of splenocytes ( P<0.05). In the acute toxicity test, EEWAR at the concentrations of 50-5 000 μg/ml had no side effects on mouse body weight and was relatively safe. Conclusions:EEWAR could promote the maturation of DCs and enhance the humoral and cellular immune responses when used as an adjuvant to OVA. It was safe in a certain dose range. This study provided reference for further research on EEWAR as a new-generation adjuvant.

Objective To explore the early diagnosis and treatment of Van Wyk-Grumbach syndrome (VWGS). Methods The clinical data of a child with VWGS were retrospectively analyzed. The related literatures were reviewed. Results Nine-year-old female presented with growth retardation and obesity for 3 years, combined with breast development for 6 months and vaginal bleeding for 3 month. The level of free thyroxine (FT4) was 0.46 ng/dL and thyrotropin (TSH)>150 mIU/L. The GnRH stimulation test showed that the gonad axis was not activated. The serum prolactin and estradiol were significantly increased. Bone age was delayed. Color Doppler ultrasound showed enlarged uterus and enlarged ovary, and ovarian cyst was seen. Pituitary MRI showed hyperplasia of the pituitary gland. The patient received the treatment of Euthyrox, and 2 months later, thyroid function was back to normal, ovaries were significantly reduced by reexamine of color doppler ultrasound, body weight was reduced by 6 kg, and there was no vaginal bleeding. Three months later, both ovaries returned to normal size, and pituitary MRI showed hyperplasia of adenohypophysis was improved. After 6 months, both of uterus and ovary were turn to normal size, ovarian cyst disappeared, and pituitary MRI showed normal. Conclusions VWGS is a rare complication of severe primary hypothyroidism untreated for long time and it mainly occurs in adolescent girls. Thyroid replacement therapy is effective.

3) in control group (χ2=5.42,P=0.02).Conclusions Gemcitabine chemotherapy combined with HIFU treatment was more effective for pancreatic tumor growth control and pain relief without more adverse events,which was effective and safe.

To investigate the chemical constituents of hemostatic extract of Pyrola calliantha H. Andres, the extract was subjected to chromatographic separation and purification. Along with some known compounds, a new naphthaquinone derivative was isolated and identified as 2-(1, 4-dihydro-2, 6-dimethyl-1, 4-dioxo-3-naphthalenyl)-3, 4, 5-trihydroxylbenzoic acid by physicochemical and spectroscopic analysis.

OBJECTIVE: To examine and determine the contents of endothelin (ET) and nitric oxide (NO) in plasma, and to observe the effects of gastrodin injection on blood pressure, ET and NO levels in old patients with refractory hypertension. METHODS: A total of 63 old patients with refractory hypertension, 30 patients with common hypertension and 30 healthy people were included. The contents of ET and NO in plasma of patients in different groups and healthy people were examined and analyzed. The old patients with refractory hypertension were randomly divided into two groups: gastrodin-treated and routine treatment groups. Besides conventional hypotensive drugs, intravenous drip infusion of 1 000 mg gastrodin was administered to the patients in gastrodin-treated group for two courses (4 weeks), while the patients in routine treatment group were treated only with conventional hypotensive drugs. The changes of blood pressure, ET and NO levels before and after treatment in different groups were measured. RESULTS: The plasma level of ET in refractory hypertension group was higher than that in common hypertension group (t=3.27, P=0.008), while the level of NO was lower (t=-3.81, P=0.002). The systolic pressure and pulse pressure difference in gastrodin-treated group were decreased significantly after one course of treatment (t=1.85, P=0.03; t=1.74, P=0.04). The level of ET in gastrodin-treated group decreased gradually after treatment, but there were no significant difference between before treatment and two courses of treatment, while the level of NO in gastrodin-treated group was increased after treatment (t=-2.70; P=0.04). CONCLUSION: Gastrodin injection is beneficial to old patients with refractory hypertension, and can improve the balance of ET and NO levels in plasma.

Objective To study the reliability and validity of the Chinese version of an instrument for assessing quality of life after traumatic brain injury (QOLIBRI).Methods A total of 49 patients with traumatic brain injury were studied.The test-retest reliability of the instrument was examined by computing intraclass correlation coefficients and its internal consistency was examined using Cronbach's α statistic.Its criterion-related validity was examined using reference instruments such as the HADS,SF-36 and Glasgow outcome scale-extended (GOSE).The Kaiser-Meyer-Olkin measure and Barlett's test of sphericity as well as factor analysis were used for construct validity testing.Results The test-retest reliability of the QOLIBRI subscales was stable,with Pearson's correlation coefficients ranging from 0.906 to 0.987.Good internal consistency was demonstrated by the Cronbach's αs ranging from 0.498 to 0.921.The Kaiser-Meyer-Olkin measure of QOLIBRI was 0.813.Factor analysis yielded four factors,and their cumulative contribution was 68.4％.The QOLIBRI scores were highly negatively correlated with HADS scores,moderately correlated with GOSE scores and positively correlated to SF-36 scores.Conclusions The Chinese QOLIBRI instrument has good reliability and validity.