Objective To investigate the effect of being waked up on bispectral index (BIS) and auditory evoked potential index (AEPI) during emergence from propofol administered by TCI.Methods Twenty ASAⅠorⅡpatients aged 31-63 yrs weighing 52-70 kg undergoing elective cholecystectomy using fiberoptic laparoscope under propofol anesthesia administered by TCI were enrolled in the study. During induction of anesthesia the initial target effect-site concentration of propofol was set at 1.0?g?ml-1 and was increased by 1.0?g?ml-1 until 6.0?g?ml-1 step by step. The BIS and AEPI values and hemodynamic variables were recorded at each target effect-site concentration during induction of anesthesia. At the end of operation the infusion of propofol was stopped and the name of the patient was called loudly every minute asking the patient to open his/her eyes. The BIS and AEPI values were recorded 1 minute before and after the eyes opened on command. Results Both BIS and AEPI correlated closely with target effect-site concentration of propofol during induction of anesthesia. During emergence from propofol at the end of operation the BIS value was 69?7 and 72?10 at 1 min before and after the patients opened eyes on command; while the mean AEPI value was 29.8?6.0 and 73.9?5.9 respectively. The increase in AEPI value was significantly larger than the increase in BIS value. Conclusion Both BIS and AEPI correlate closely with target effect-site concentration of propofol during induction of anesthesia. During emergence from propofol the AEPI is more sensitive to being waked up on command.

Objective To evaluate clinical application of arterial embolotherapy on hyperthyroidism. Methods 11 patients with hyperthyroidism were performed with thyroid superior and inferior arterial super selective arteriography and interventional embolization by polyvinyl alcohol(PVA),gelfoam particles and wool gianturco coil. Results The procedures were succeeded in 11 patients. After the embolotherapy , the thyroid function gradually returned to normal level in 10 patients. The symptom was not controlled satisfactorily in 1 patient, who underwent the right thyroid inferior arteries superselective arteriography and interventional embolization, and then the thyroid function gradually returned to normal level. No serious complications occurred in all patients. Conclusions Arterial embolotherapy is a safe, simple,symptomless, reliable and very effective for treatment of hyperthroidism.

Objective To investigate the feasibility and efficacy of combined preoperative chemora- diation program followed by radical surgery in bulky early or moderate uterine cervical cancer.To determine the incidence and predictive value of rasidual disease in the radical hysterectomy specimens after CCR. Methods Thirty-five patients with uterine cervix carcinoma from January 2001 to September 2003 were treated with preoperative external beam radiotherapy to 3060 cGy in 4 weeks.Patients received concurrent continuous infusion cisplatin(20 mg/m~2)on day 1,8,15,22 of four weeks and 5-Fu(4 g)chemotherapy during the first four days and the last four days of the radiation course.Radical surgery was not performed until 4 weeks after the completion of the preoperative treatment.Results Clinical response was 85.71%,complete response,54.29 %;partial response,31.43 %.The analysis of the surgical specimens showed 19 patients had revealed residual disease and 16 patients had revealed no residual disease,the complete pathological response hed been 45.71%.Three-year survival rate was 87.14 % in 35 patients.Three-year survival rate of residual disease patients(76.32 %)was significantly lower than that of no residual disease(100.0 %)(P=0.0358). Three-year survival of cervical stromal invasion less than 1/2 and invasion extra stromal was 100.0 %, 41.67 % respectively(P=0.0109);three-year survival of lymph-vascular space involvement and no lymph- vascula space involvement was 0,85.29 %,respectively(P=0.0148).Conclusion Combined preoperative chemoradiation program followed by radical surgery in bulky early or moderate cervical cancer could reveal an effective efficacy with a tolerant complication.Residual disease is an independent and strong predictive factor.

Object To develop the gene resources of Jilin Shuangyang sika deer,a rare animal in China and reveal the pharmacological functions of deer medicinal material on the molecular level. Methods A cDNA plasmid library of spleen cells derived from Shuangyang sika deer was set up. Some housekeeping genes were cloned and analyzed. Results One of the cDNA sequences and it's deduced amino acid sequence in deer share high homology with a group of cDNA sequences encoding ribosomal protein L27 in human,rat,Canis and mouse and their corresponding amino acid sequence respectively. It has the completed open reading frame as well. Conclusion A novel full-length cDNA encoding Shuangyang sika deer ribosomal protein L27 is discovered. This sequence has been deposited in Genbank under accession number AF373231.

BACKGROUND:With the development of genetic engineering and tumor molecular biology, gene therapy for tumors has become a new treatment modality.
OBJECTIVE:To explore the effect of the KDR gene silencing on the proliferation and invasion capacity of breast cancer MCF-7 cel s.
METHODS:Interfering RNA (siRNA) sequences for smal molecule KDR gene was designed and transferred into human breast cancer MCF-7 cel s. Then, RT-PCR and western blot assay were used to detect the KDR mRNA and protein expression. Flow cytometry, cel counting kit-8 test and Transwel test were employed to detect the cel cycle, proliferative capacity and invasion capacity of breast cancer MCF-7 cel s after the KDR gene silencing.
RESULTS AND CONCLUSION:After 48 hours of KDR silencing, the mRNA and protein expressions of KDR in MCF-7 cel s were decreased obviously;MCF-7 cel s arrested at G0/G1 stage and the number of cel s at S stage was reduced. Cel proliferation was inhibited significantly. The amount of cel s passing through the filtering membrane became less. After KDR gene silencing, the proliferation and invasion of breast cancer MCF-7 cancer stem cel s were inhibited remarkably, indicating that KDR gene silencing may be a new target for the effective treatment of breast cancer.

Animal Feed ; Animals ; Cysteamine ; Female ; Goats ; Mammary Glands, Animal ; growth & development ; physiology

Objective To investigate the pathogenesis,high risk factors,clinical characteristics,methods of diagnosis and treatment,and prognosis of vaginal intraepithelial neoplasia (VAIN).Methods The clinical data of thirteen cases of VAIN treated in Zhejiang Provincial Cancer Hospital dated Mar.2002 through Dec.2008 were reviewed and analyzed retrospectively.Results Twelve of 13 VAIN cases were performed the human papillomavirus(HPV) detection with 92% (11/12) HPV positive rate.None of the cases shown specific clinical manifestation.Among the 13 cases,6 of them accompanied with cervical cancer,4 cases with cervical intraepithelial neoplasia (CIN ),and 3 cases with vulvar intraepithelial neoplasma (VIN).Five cases synchronously diagnosed with cervical lesion and 3 with vulva lesion were underwent surgery,while the other 5 cases were diagnosed metachronously.Among 8 cases underwent surgery,1 case with CIN underwent argon plasma coagulation (APC) after surgery,1 case with the positive edge of VIN underwent APC.During follow up,1 case with locally advanced cervical cancer underwent radiotherapy again,3 cases with VAIN received APC,while 1 cervical cancer cases with VAIN received no treatment.The average follow-up time was 25.6 months (range 6-87 months).Two cases died of cervical cancer metastasis.The other 11 cases were normal and still alive.None of them progressed to invasive carcinoma.Conclusions The main reason of VAIN is HPV infection.There are not specific clinical manifestations,usually diagnosed when reviewing cervical or vulva lesions and rarely progressed to invasive carcinoma.The main treatment of VAIN is surgery with the adjuvant treatment of APC.

Objective To study the polysomnography(PSG) indexes which can reflex the severity of anoxia in obstructive sleep apnea-hypopnea syndrome(OSAHS) more comprehensively. Methods Sixtythree OSAHS patients' PSG indexes, including the apnea-hypopnea index (AHI), apnea time/total sleeping time (AM), hypoventilation time/total sleeping time (HM), mean oxygen saturation (MSaO2),the lowest oxygen saturation (LSaO2) and the longest apnea time (LAT) were analyzed retrospectively. Results In patients with OSAHS,the AHI was positively correlated with AM, HM and LAT(P ＜ 0.01 or ＜ 0.05 ), and was negatively correlated with MSaO2 and LSaO2(P ＜ 0.01 ). The AHI had linear regression relationship with AM,HM and LSaO2 (F =202.264,P ＜0.01). Conclusions Among the PSG indexes,AM is intimately associated with AHI. Therefore,compared with LSaO2,AM and HM can reflex the severity of anoxia in patients with OSAHS more accurately.

Objective To evaluate arterial embolization in the treatment of hyperthyrodism.Methods The catheterization of the femoral artery was carried out with Seldinger's method.After super-selective catheterization of superior and inferior thyroid arteries were completed,embolization with PVA,grain of gelfoam or wreath of spring was performed.Results The procedures were succeeded in 11 cases.After the embolization,the function of thyroid gradually retuned to normal level.All of them were healed clinically.Conclusion Arterial embolization is a simple,safe,reliable and very effective treatment for hyperthyrodism.

Objective To assess the outcomes of the surgical treatment of supination external rotation trimalleolar fractures,comparing the techniques of lateral plating and antiglide plating as described previously.Methods This is a retrospective review.A total of 31 patients meeting our inclusion criteria,with supination external rotation trimalleolar fractures surgically treated between 2009 and 2011,were studied.17 patients were treated with antiglide plating,whereas the remaining 14 patients underwent traditional lateral plating.They were followed up by a period ranging from 14 to 38 months(average,26 months).The functional results were evaluated with olerud and molander scoring system described previously.All the data including time to surgery,operating room time,tourniquet time,hospital stay and ankle joint function scores,were respectively analyzed in accordance with the complete randomized design t-test.Results There was no statistically significant in time to surgery,operating room,tourniquet time,hospital stay and ankle joint functional scores.Conclusions The outcome of the surgical management of supination external rotation trimalleolar fracture is comparable with both techniques.Our data do not support one technique over the other.