Objective To observe the clinical efficacy of electroacupuncture plus electro-spoon needle-cupping in treating lumbar-dorsal myofascial pain syndrome.Method Sixty patients with lumbar-dorsal myofascial pain syndrome were randomized into group A, group B, and group C, 20 cases in each group. With same acupoints selected, group A was intervened by electroacupuncture plus electro-spoon needle-cupping, group B was by electroacupuncture, while group C was by conventional acupuncture. Visual Analogue Scale (VAS) was observed before and after treatment, and the clinical efficacies of the three groups were compared.Result After treatment, VAS scores were significantly different from those before treatment in the three groups. (P<0.05). After treatment, the VAS scores in group A and B were significantly different from that in group C (P<0.01,P<0.05); the VAS score in group A was significantly different from that in group B (P<0.05). The total effective rate was 95.0% in group A, versus 90.0% in group B and 80.0% in group C, and the total effective rate of group A was significantly different from that of both group B and C (P<0.05).Conclusion Electroacupuncture plus electro-spoon needle-cupping is an effective method in treating lumbar-dorsal myofascial pain syndrome.

Objective To observe the effect of repetitive transcranial magnetic stimulation (rTMS) and functional magnetic stimulation over spinal cord (SC-MS) on motor function recovery after spinal cord hemisection in rats. Methods T10 spinal cord hemisection model was made. The stimulation (5×10 s bursts of 5 Hz at 35% maximal stimulator output, each burst separated by a 2 m interval) was delivered daily, 5 d per week for 2 weeks. The treatment began at 4 d after surgery for rTMS group and SC-MS group. Motor function recovery was assessed with Basso, Beattie & Bresnahan locomotor rating scale (BBB) and the Horizontal Ladder test. The tibialis anterior was surgically removed at 38 d after spinal cord injury for calcitonin gene-related peptide (CGRP) iummunohistochemical staining. Results The scores of BBB and Horizontal Ladder test were significantly more at 17 d after spinal cord hemisection in rTMS group than before treatment and in spinal cord injury group (P<0.05). In SC-MS group, the scores of BBB and Horizontal Ladder Test were significantly more 10 d after SCI than before treatment and in SCI group (P<0.05). The score of Horizontal Ladder test of SC-MS group was more 10 d after SCI than that in the rTMS gourp (P<0.05). The expression of CGRP on motor endplates of the tibialis anterior in rTMS group and SC-MS group were more than those of SCI group (P<0.01). Conclusion rTMS and SC-MS in acute stage can improve the motor function recovery and muscle plasticity after spinal cord hemisection in rats. The magnetic stimulation can facilitate the recovery of motor function after spinal cord hemisection in rats.

Humans ; Organometallic Compounds ; chemistry ; metabolism ; toxicity

Arsenic and its species in dry seafood products(DSPs) Kelp, Sargassum fusiform, Laver, Enteromorpha prolifera, were systematically determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP-MS). It was found that the of arsenic species in botanic DSPs are three unknown arsenicals(U_2, U_3 and U_4), which are speculated as arsenosugars and take approximate 63.5% 92.7% of all arsenic species detected. These arsenosugars were indentified as dimethylarsinic(DMA)-glycerol ribose, DMA-phosphate ribose and DMA-sulfate ribose by their accuracy molecular weight 329.0599, 483.0738 and 409.0162, using direct confirmatory of HPLC-TOF-MS. The quantitative analysis of these arsenic species shows that although the total arsenic exceeds the National Standard value, most of the marine plants DSPs are safe to eat. The main component in most marine plants is DMA-sulfate ribose(51.1%-80.3%), while the main component of Kelp is DMA-phosphate ribose(48.9%). The sum of those three arsenosugars was 63.5%-92.7% of peak areas in all arsenic species detected.

Objective To explore the splenic macrophages phenotype and secretory function of mice with periodontitis, so as to explore effects of periodontitis on macrophages. Methods 22 mice were randomly divided into periodental ligation group (group P10d ) and pseudo periodental ligation control group (group C), with 11 mice in each group. The experimental periodental ligation on mice lasted for 10 days before they were sacrificed. Flow cytometry was applied to detect the expression of M1 and M2 in mononuclear macrophages. Real-time PCR was applied to detect the relative expression of pro-inflammatory cytokines IL-1β and anti inflammatory cytokines IL-10. Results Compared with the control group C, the proportion of M1 macrophages in the periodontitis group decreased, and the ratio of M1/M2 was also decreased significantly, and IL-1β mRNA also down-regulated. Conclusions Chronic periodontal infection could down regulate the proportion of M1 macrophages , decrease ratio of M1/M2 and the expression of inflammatory cytokines IL-1βmRNA.

Caenorhabditis elegans (C.elegans), has developed into an useful model for biomedical research, particularly in the targets of novel drugs and the related mechanism.C.elegans, combining with cost-effective cultivation, simple biological structure, make for high-throughput screenings in vivo model.In this review, we explore the feasibility of C.elegans in drug activity evaluation of complex systems.Because of the body independence of C.elegans, it provided good platform for the efficacy and mechanism of research on new drugs as well as Chinese herbal extract in vivo, showing a good prospect.

Objective To explore the clinical curative effect and nursing measures of damaged skin and ganglion radiofrequency thermal condensation in the treatment of elderly primary trigeminal neuralgia.Methods 144 elderly patients with trigeminal neuralgia were randomly divided into observation group and control group,72 cases in each group.The control group was given percutaneous and ganglion radiofrequency thermal condensation damage to surgical treatment,the observation group was given in combination with the comprehensive nursing intervention.The clinical treatments of the two groups were observed.Results After the intervention,the anxiety self -assessment rating scale, depression self rating scale score of the observation group were (41.88 ±1.04)points,(44.19 ±2.15)points which of the control group after the intervention were (45.86 ±2.47)points,(51.08 ±3.97)points,the differences between the two groups were statistically significant (t =12.601,12.949,all P <0.05).In observation group,the length of hospital stay,visual analog pain score,nursing satisfaction score were (16.71 ±1.64)d,(1.48 ± 0.51)points,(94.58 ±2.43)points,which of the control group were (23.86 ±2.96)d,(3.26 ±1.14)points, (88.17 ±5.87)points,the differences between the two groups were statistically significant (t =17.928,12.093, 8.561,all P <0.05).The incidence rate of postoperative complications of the observation group was 5.56%,which of the control group was 16.67%,the difference between the two groups was statistically significant (χ2 =4.500,P <0.05).Conclusion Damaged on percutaneous and ganglion radiofrequency thermal condensation in treatment of elderly patients of primary trigeminal neuralgia with comprehensive nursing intervention can effectively relieve psycho-logical negative emotions,shorten hospitalization time,the patients'pain score,reduce the postoperative complications, it is worthy of popularization and application in clinic.

Objective:To study the expression of B-1a cells in mice with obesity and periodontal infection.Methods:Mouse models of diet induced obesity combined with experimental periodontitis were established,the expression of CD5 protein,anti-collagenⅠ antibody(anti-Col-Ⅰantibody) and IL-10 protein was examined in mouse jaw bone and spleen by immunohistochemistry and Western blot;The mRNA expression of CD5,anti-Col-Ⅰantibody and IL-10 in mouse jaw bone was detected by real time quantitative PCR.Results:The mRNA and protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in jaw bone in periodontitis group were significantly higher than those in control group(P<0.001).The protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in spleen in obesity group were significantly higher than those in standard group(P<0.05).The protein expression of anti-Col-Ⅰantibody in spleen in standard accompanying periodontal ligature group was significantly higher than that in standard without periodontal ligature group(P<0.05).Conclusion:B-1a cells are activated in the early stage of obesity and periodontal inflammation with a certain pathological significance and without interation between the two inflammatory states in the pathological mechanism.

Objective To investigate the effects of Thalidomide(THD)on transdifferentiation of human fetal lung fibroblast(HFL-F) to myofibroblast(MF) induced by Transforming Growth Factor-?1(TGF-?1) and the effects on trans differentiated MF.Methods HFL-F to MF trans-differentiation was induced with 5 ?g/L TGF-?1.The effect of 50 ?g/L THD on HFL-F to MF transdifferentiation was evaluated by measuring hydroxyproline(HYP) content with alkaline hydrolysis colorimetry,?-smooth muscle actin(?-SMA) protein with Western Blot,?-SMA and collagen Ⅲ(COL Ⅲ) mRNA with semiquantitative RT-PCR.Results THD inhibited TGF-?1 induced up-regulation of HYP and COLⅢ mRNA expressions(all P0.05).For HFL-F treated with 5 ?g/L TGF-?1 for 96 h,THD inhibited COLⅢ mRNA expression(P

AIM To observe the effects of Shangke Huangshui Gauze (Scutellariae Radix,Coptidis Rhizoma,Phellodendri chinensis Cortex,etc.) on postoperative knee joint pain,swelling degree and functional recovery in patients following total knee arthroplasty (TKA).METHODS Eighty patients receiving TKA and routine care in Foshan Hospital of Traditional Chinese Medicine (FHTCM) from 2015.1 to 2016.1 were randomly and equally divided into observation group and control group.The patients in the observation group were given external use of Shangke Huangshui Gauze.The swelling degree,the score of VAS,and the score of HSS in the two groups were compared.RESULTS On the 7th,14th and 30th days after the surgery,the swelling degree in the observation group was lower than that in the control group.On the 7th,14th days,VAS scores in the observation group were significantly lower than those in the control group.In the 1st,3rd and 6th months after the surgery,patients in the two groups got higher HSS scores,but HSS scores in the observation group were significantly higher than those in the control group.All the differences had statistical significances (P ＜ 0.05).CONCLUSION The long-term external use of Shangke Huangshui Gauze can effectively relieve postoperative pain and swelling,and improve knee joint function in patients following TKA.