Objective To investigate the response of cryopreserved ovarian tissue after autologous implantation in mouse stimulated with gonadotrophin.Methods Thirty six female mice were randomly divided into three groups,with 12 mice in each group.In group of fresh ovarian tissue,fresh ovarian tissue was implanted into kidney capsule of mice:in group of cryopreserved ovarian tissue,ovarian tissue was implanted into kidney capsule of mice after cryopreserved by vitrification for two weeks.We investigated the response of ovarian tissue two weeks later after autologous implantation stimulated with gonadotrophin.Immunohistochemistry staining method was used to observe the expression of follicle stimulating hormone receptor.Results Before and after stimularian with gonadotrophin,the mature follicle rate of group of fresh ovarian tissue was 2.3%and 4.2%.that of group of cryopreserved ovarian tissue was 2.3%and 4.0%,and that of group of control was 2.6%and 5.8%.Regarding the percentages of mature follicle.there were significant differences after stimulation with gonadotrophin(P＜0.05).After stimulating with gonadotrophin the percentages of mature follicle were the same in the fresh tissue group,cryopreserved tissue group and control group(P＞0.05).The integrated optical density of follicle stimulating hormone receptor of fresh ovarian tissue in antrofollicle and pre-antrofollicle were 9408±2777 and 4531±1903.that of cryopreserved ovarian tissue were 9175±3093 and 4808±1386.and that of the control ovarian tissue were 8838±2064and 5516±1136 respectively.There was no significant difference between any two groups(P＞0.05).Conclusion The follicle stimulating hormone receptor is preserved by cryopreservation and transplantation,small pieces of ovarian tissue response to gonadotropin stimulation is normal.

AIM To observe the effects of Shangke Huangshui Gauze (Scutellariae Radix,Coptidis Rhizoma,Phellodendri chinensis Cortex,etc.) on postoperative knee joint pain,swelling degree and functional recovery in patients following total knee arthroplasty (TKA).METHODS Eighty patients receiving TKA and routine care in Foshan Hospital of Traditional Chinese Medicine (FHTCM) from 2015.1 to 2016.1 were randomly and equally divided into observation group and control group.The patients in the observation group were given external use of Shangke Huangshui Gauze.The swelling degree,the score of VAS,and the score of HSS in the two groups were compared.RESULTS On the 7th,14th and 30th days after the surgery,the swelling degree in the observation group was lower than that in the control group.On the 7th,14th days,VAS scores in the observation group were significantly lower than those in the control group.In the 1st,3rd and 6th months after the surgery,patients in the two groups got higher HSS scores,but HSS scores in the observation group were significantly higher than those in the control group.All the differences had statistical significances (P ＜ 0.05).CONCLUSION The long-term external use of Shangke Huangshui Gauze can effectively relieve postoperative pain and swelling,and improve knee joint function in patients following TKA.

Objective To explore the application of diffusion tensor imaging in the assessment of ischemic muscle in hind-limb ischemia model of rabbit. Methods Excision of femoral artery in unilateral hind limb was done in 14 New Zealand white rabbits and ischemic model were established in 12 rabbits. Three (12 rabbits),10(10 rabbits) ,28(7 rabbits),56(5 rabbits) days after the model establishment, DTI scan was performed on bilateral hind limbs in each of the models, respectively and, λ_1,λ_2,λ_3, ADC and FA values were measured. Histological analysis was also performed at these time points. Pared t test was used to compare the differences of these indexes in bilateral hind limbs. Results Following femoral artery excision, a rapid ascending of ADC,λ_2,λ_3 values with sharply reduced FA value was observed in ligated hind-limb, which reached maximal on 3 days post-excision (ADC_(ligated)= 1.72±0.16, ADC_(unligated)= 1.53±0.16, t = 6.48, P < 0.01 ; λ_(2 ligated)= 1.70±0.15, λ_(2 unligated)= 1.51±0.06, t=10.87, P < 0.01 ; λ_(3 ligated_ =1.17±0.12, λ_(3 unligated)= 0.88±0.12, t=6.67, P < 0.01 ; FA_(ligated)= 0.24±0.04, FA_(unligated) =0.39±0.03, t = -10.61 ,P <0.01) and histologic analysis revealed the severest muscle damag at that time. Ischemic muscle recovered very slowly during the first 10 day post-excision accompanied with reduction of ADC , λ_2, λ_3 values, however there was also difference of ADC,λ_2, λ_3 values between ligated and nonligated limbs except λ_1 ( ADC_(ligated) = 1.65±0. 16, ADC_(unligated)= 1.50±0.12, t =6.42, P <0.01 ; λ_(2 ligated) = 1.62±0.32, λ_(2 unligated) =1.48±0.31, t=5.09, P < 0.01) ; λ_(3 ligated)= 1.11±0.13, λ_(3 unligated)= 0.85±0.09, t=6.26, P <0. 01;λ_(1 ligated)=2.20±0.21, λ_(1 unligated) =2.18+0.20, t=0.87, P=0.40). After 28 days, ADC and λ_3 returned to normal (ADC_(ligated)= 1.51±0. 16, ADC_(unligated)= 1.55±0.14, t=-1.35, P=0.23 ; λ_(3 ligated) =0.95±0. 10, λ_(3 unligated)= 0.92±0.06, t=1.70, P=0.14), but λ_2 and FA of ligated limb were still different from those of nonligated limb (λ_(2 ligated)= 1.45±0.23, λ_(2 unligated)= 1.52±0. 95, t=-3.56, P=0.012; FA_(ligated)=0.35±0.02, FA_(unligated)=0.40±0.03, t=-3.83, P<0.01). After 56 days, all parameters retuned to normal(ADC_(ligated) =1.57±0.18, ADC_(unligated)=1.58±0.23, t=-0.71, P=0.52; λ_(1 ligated) =2.18±0.18, λ_(1 unligated)=2.24±0.14, t=-0.22, P=0.10; λ_(2 ligated)=1.64±0.13, λ_(2 unligated)=1.59±0.15, t=0.89, P=0.42; λ_(3 ligated)=0.89±0.1,λ_(3 unligated)=0.91±0.07, t=- 1.64,P= 0.18; FA_(ligated)= 0.39±0. 03, FA_(unligated)= 0.41±0.02, t=-0.83, P=0.47). Conclusion DTI is a quantitative and relatively accurate technique to assess time-dependent changes of ischemic muscle in hindlimb ischemia model of rabbit.

Animals ; Humans ; Lymph Nodes ; pathology ; Lymphangiogenesis ; physiology ; Lymphatic Metastasis ; Lymphatic Vessels ; metabolism ; pathology ; Neoplasms ; metabolism ; pathology ; Vascular Endothelial Growth Factor C ; metabolism ; physiology ; Vascular Endothelial Growth Factor D ; metabolism ; physiology

OBJECTIVE To prepare the Graves disease (GD) mouse model through porcine thyroid globulin (PTG) injection and investigate the morbidity and stability of the model. METHODS C57BL6/N mice in model group received multi-point subcutaneous injection of PTG 25μg each week,six times in all. After the end of immunization,their heart rate and oxygen consumption were measured and serum triiodothyronine(T3)level was determined every two weeks. A model was considered successful if serum T3 level was higher than x+3s of the control group. Observation of the model lasted 12 weeks. At the 12th week,spleen and thymus gland indices,serum thyroid globulin antibodies and thyroid peroxidase antibodies were measured,and the thyroid glands were taken for pathological observation. RESULTS After six times of immunization,mice in model group showed increased heart rate(P<0.01),oxygen consumption(P<0.01)and T3 level(P<0.01)compared with control group. The morbidity was 77.7%for male mice and 88.8%for females. In addition,T3 level in model group remained higher than that in control group within 12 weeks after immunization. The T3 level tended to decrease in male mice,but remained at a relatively stable higher level in females. CONCLUSION This method is suitable for GD modeling due to its short model-making time,high morbidity and long durability.

Objective To explore the relationship of acute myeloid leukemia kayotype with chemotherapeutic effect. Methods Conventional cytogenetic technique of G-band was used to analyze in 222 acute myeloid leukemia patients. Results Eighty-six cases (42. 1%) with cytogenetic abnormalities were found in 204 patients. Excepting for M_3 patients,the favorable,intermediate and adverse groups according for MRC criteria were treated with TA or CAG schema respectively. CR rate of M_3 was obviously higher than non-M_3. The favorable and intermediate group were obviously surpassinger than adverse group in TA schema. CR rate of the intermediate group was highter than that of other groups in CAG schema. CR rate of the adverse group in two schema were all low. CAG was surpassinger than TA schema in intermediate group. Conclusion Cytogenetics is an important chemotherapeutic and prognostic factor to acute myeloid leukemia. CAG schema could be the leader selection in the de novo presby or oligoac-crementional AML patients.

Background Researches have demonstrated that dexamethasone (Dex) can induce the changes of the function and structure of trabecular meshwork cells,and latrunculin A (Lat A) can enhance the outflow of aqueous humour and therefore low the intraocular pressure.Objective The aim of the present study is to investigate the effects of Dex and Lat A on the expression of protein in human trabecular meshwork cells.Methods Human trabecular meshwork cells were primarily cultured in DMEM using expand culture method and the fifth generation of cells were used to this experiment.Dex and/or Lat A were added in medium as 10~(-6)mol/L Dex group(Dex treating for 24 hours),Dex+Lat A group(10~(-6)mol/L Dex+2mmol/L Lat A for 24 hours),Lat A group(2mmol/L Lat A for 24 hours) and DMEM culture group.Two dimensional gel electrophoresis(2 DE) was used to compare the protein expressions among these four groups.Subsequently protein spots with different intensity were selected for mass spectrometry analysis.Results Four gel patterns of two dimensional gel electrophoresis of human trabecular meshwork cells from Dex,Dex+Lat A,Lat A and control groups were obtained.A good isolated result for majority of proteins in human trabecular meshwork cells was found in all of the four groups.An obvious expression difference of proteins in human trabecular meshwork cells was seen among the different culture conditions.Twenty four kinds proteins were identified by GDPiMALDI TOF MS,including cytoskeleton related proteins,heat shock proteins,redox related proteins,and proteins participating in carbohydrate metabolism.The expressions of aldehyde dehydrogenase(ADLH)and Rab were increased in Lat A group and decreased in Dex group,but HSP27 and hCRMP2 showed the contrary outcome.Conclusion This study construct the pattern of protein expression in human trabecular meshwork cells by using 2 DE.Dex and Lat A impact the protein expressions in human trabecular meshwork cells.

Objective The purpose was to compare the biomechanical characteristics of new percutaneous guide wire and conventional wire in cadaveric spines,and to evaluate the new percutaneous guide wire's efficacy and safety in a clinical trial.Methods Compared the push-out and penetration forces of the new percutaneous guide wire and conventional wire in fresh cadaveric lumbar spines from L1 to L5.And analyzed the related complications of new percutaneous guide wire by clinical experiment.Results Push-out forces caused the spiral part of the new percutaneous guide wire to bend or spread,so as to resist the anterior migration of the guide wire.The mean push-out forces of the new percutaneous guide wire and conventional wire were (15.5-+ 1.9) N and (5.7 ± 0.8) N respectively (P ＜ 0.01),and the mean penetration forces were (69.1 ±4.2) N and (37.1 ±4.8) N respectively (P ＜0.01).There was no wire breakage or anterior-wall penetration in the clinical trial of 222 new percutaneous guide wire.Conclusion The mean push-out and penetration forces of the new percutaneous guide wire were approximately 2 to 3 times greater than those of conventional wire.The new percutaneous guide wire effectively prevented guide-wire anterior migration and penetration of the anterior vertebral-body wall.The new percutaneous guide wire device could effectively improve the safety of percutaneous pedicle screw insertion procedures for patient with osteoporosis.

Objective The aim of this study was to provide methods for predicting ideal trajectory and position of C1 lateral mass screw (C1 LMS)from plain radiographs.Methods A total of 40 consecutive subjects (with 79 screws)who had undergone C1 LMS placement were enrolled.To evaluate the C1 LMS position,the positions of screw head and tips on anteroposterior radiographs,screw length,and height on lateral radiograph were graded as 0°,Ⅰ°,and Ⅱ°,respectively.On the postoperative computed tomography images,the lateral mass (LM) perforation,screw-thread engagement percent(%),bicortical fixation,extruded screw length,and violation of adjacent joints were analyzed. Results Screws with tip located medial to LM(tip 0)showed LMperforation in all cases.Polyaxial head located within the LM(head 0)or crossing the lateral margin of the LM(head Ⅰ)showed no LMperforation.Screw-thread engagement percent was the highest with head Ⅰ-tip Ⅰ (medial half of LM)position (97.6%),followed by head 0-tip Ⅰ (90.5%)and head Ⅰ-tip Ⅱ (lateral half of LM)(86.4%). Screws longer than the posterior half of C1 anterior arch (AA)showed bicortical fixation in all cases with mean extruded screw length of 1.9 mm.Adjacent joint was not violated in 98%,with the screw height below half of C1 AA.Conclusion On an anteroposterior radiograph, a C1 LMS with the screw head located on the lateral margin of the LMand with the screw tip in the medial half of the LMresulted in the safest and longest trajectory.On lateral radiograph,a screw tip that is placed within the anterior-inferior quadrant of the C1 AA results in safe bicorti-cal fixation without injury to the adjacent structures.These plain radiographic findings may be helpful bothpostoperatively and intraoperatively for assessing the trajectory and length of the screw.

Objective To explore the relationship of acute myeloid leukemia kayotype with chemotherapeutic effect. Methods Conventional cytogenetic technique of G-band was used to analyze in 222 acute myeloid leukemia patients.Results Eighty-six cases(42.1%) with cytogenetic abnormalities were found in 204 patients.Excepting for M3 patients,the favorable,intermediate and adverse groups according for MRC criteria were treated with TA or CAG schema respectively. CR rate of M3 was obviously higher than non-M3.The favorable and intermediate group were obviously surpassinger than adverse group in TA schema.CR rate of the intermediate group was highter than that of other groups in CAG schema. CR rate of the adverse group in two schema were all low. CAG was surpassinger than TA schema in intermediate group.Conclusion Cytogenetics is an important chemotherapeutic and prognostic factor to acute myeloid leukemia. CAG schema could be the leader selection in the de novo presby or oligoaccrementional AML patients.