1.Research updates on vesicle-associated membrane protein-associated protein 33.
Chinese Journal of Pathology 2011;40(11):790-792
Amyotrophic Lateral Sclerosis
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genetics
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Animals
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Biological Transport, Active
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Bipolar Disorder
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genetics
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Glucose Transporter Type 4
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metabolism
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Hepacivirus
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physiology
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Humans
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Neoplasm Metastasis
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Neoplasms
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metabolism
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Point Mutation
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Polymorphism, Single Nucleotide
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R-SNARE Proteins
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metabolism
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Tissue Distribution
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Transport Vesicles
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physiology
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Vesicular Transport Proteins
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chemistry
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genetics
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metabolism
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physiology
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Virus Replication
2.Effect of preoperative regional artery chemotherapy on gastric carcinoma
Wen HUANG ; Yu ZHAO ; Ling LUO
Journal of Third Military Medical University 2003;0(18):-
Objective To evaluate the effects of preoperative regional artery chemotherapy (PRACT) on gastric carcinoma. Methods Celiac arteriography was performed by transfemoral artery catheter according to Seldinger's method and superselective catheterization proceeded to the supplying artery of focus of lesion. Antineoplastic agents of Fluorodeoxyuridine (FUDR)(0.8 g/m 2), epirubicin(40 mg/m 2), oxaliplatin(80 mg/m 2) were infused into regional artery by a single administration. The specimens from 14 cases of gastric carcinoma(GC) who received PRACT were separated into pre operation group and post operation group. Both microscopic study to observe the necrosis of cancer cell and TUNEL(TdT mediated dUTP nick end labeling) method to detect the state of apoptosis were performed respectively in the two groups. Correlation of apoptosis index (AI) between the two groups, as well as correlation between the situation of necrotic tumor cell and the blood vessel, was analyzed. Results The severity of the necrosis of tumor cell was significantly higher in the PRACT group than that in the untreated group ( P
4.Whether amnion membrane preserved in Honghua injection can prevent tendon adhesion following transplanting into foot flexor tendon?
Wenhai ZHAO ; Dong YU ; Hui WEN ; Dongming HAO ; Changwei ZHAO
Chinese Journal of Tissue Engineering Research 2010;14(5):937-940
BACKGROUND: Recent studies have reported that more and more methods were used to prevent and cure tendon adhesion following tendon rupture by repairing tendinous sheath. Especially, amnion membrane is commonly used to effective prevent and cure adhesion and promote healing of biomembrane; however, the effect on tendon adhesion needs to be further studied. OBJECTIVE: To verify the efficacy of amnion membrane preserved in Honghua injection on preventing and curing tendon adhesion following transplanting into foot flexor tendon. METHODS: Bilateral foot flexor tendons of 32 healthy mature chickens were cut off. By anastomosis, amnion membrane preserved in Honghua injection was transplanted into left foot flexor tendon, considering as experimental group. Right foot flexor tendons were randomly divided into two groups: blank control group, anastomosis was performed alone; positive control group, amnion membrane not preserved in any injections was transplanted. At 4 weeks after fixation by plaster cast, sliding function of tendon was detected using biomechanics, and local samples were obtained for histopathological observation. RESULTS AND CONCLUSION: In the experimental group, broken end of left tendon was well healed; fiber tissues were formed surrounding tendon; tissue adhesion was not observed surrounding tendon. Proliferative quantity and adhesion of fiber tissues, as well as content of hydroxyproline in the experimental group were significantly less than in the blank control and positive control groups (P < 0.05, P < 0.01); total inflexion angle of articulationes digitorum pedis and slipping distance of flexor digitorum profundus tendon in the experimental group were significantly greater than in the blank control and positive control groups (P < 0.05, P < 0.01). The results indicated that amnion membrane preserved in Honghua injection might prevent tendon adhesion and effectively promote tendon healing.
5.Microsatellite instability and loss of heterozygosity on chromosome 3p,9p and 14q in renal cell carcinoma
Hai-Tang CHEN ; Wen-Jun CHANG ; Hong-Yu YU ; Jin-Feng ZHAO ; Guang-Wen CAO ;
Academic Journal of Second Military Medical University 1985;0(06):-
Objective:To investigate frequencies of microsatellite instability(MSI)and loss of heterozygosity(LOH)in renal ceil carcinoma(RCC),and to discuss the relationship of clinicopathological characteristics of RCC with MSI and LOH. Methods:Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations(MSI and LOH)in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction- polyacrylamide gel elect rophoresis-ethylene dibromide(PCR-PAGE-EB)staining and sequencing.Results:The frequency of MSI could reached 61.3% and that of LOH could reach 54.8%.The highest frequency of MSI was at locus of D9S168(32.3%);the highest frequency of LOH was at locus of D3S1289(21.4%).No correlation was found between MSI or LOH and the patients' age,sex,pathology type and metastastis,except that MSI was correlated with TNM stage of RCC(P
6.A review on the research progress of the computer-aided detection of pulmonary nodule.
Yu ZHAO ; Wen LU ; Yuanjun WANG ; Shengdong NIE
Journal of Biomedical Engineering 2014;31(5):1172-1177
Computer-aided detection (CAD) of pulmonary nodule technology can effectively assist the radiologist to enhance lung nodule detection efficiency and accuracy rate, so it can lay the foundation for the early diagnosis of lung cancer. In order to provide reference for the scholars and to develop the CAD technology, we in this paper review the technology research and development of CAD of the pulmonary nodules which is based on CT image in recent years both home and abroad. At the same time, we also analyse the advantages and shortcomings of different methods. Then we present the improvement direction for reference. According to the literature in recent years, there still has been large development space in CAD technology for pulmonary nodules. The establishment and improvement of the CAD system in each step would be of great scientific value.
Computer Systems
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Diagnosis, Computer-Assisted
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Humans
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Lung
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pathology
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Lung Neoplasms
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diagnosis
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Software
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Tomography, X-Ray Computed
7.Left atrial volume and function evaluation by dual source CT:a comparison with real-time three-dimensional echocardiography(RT-3DE)
Zhaoying WEN ; Ying ZHAO ; Xingmei ZHANG ; Wei YU ; Zhaoqi ZHANG
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
0.05). The interobserver variability in DSCT measurements was lower than in RT-3DE measurements in indexed left atrial volume and function. Conclusion DSCT can provide not only the information of coronary artery,but also highly reliable measurements of left atrial phasic volume and function at the same time.
8.Cost-minimization Analysis on 2 Drug Therapeutic Regimens for Children Pneumonia
Xuqiang HUANG ; Li DENG ; Huihong WEN ; Jialu YU ; Chang'An ZHAO ;
China Pharmacy 2005;0(20):-
0.05),but the total expenses and antibiotic cost of the medical treatment in group B were obviously lower than those in group A(P
9.Effects of endogenous NO on sensitivity to chemotherapy in human breast cancer cell line
Wei TU ; Jian WEN ; Wanqing QIAO ; Man ZHAO ; Zuofu YU
International Journal of Surgery 2010;37(1):32-35
Objective To evaluate the effects of endogenous NO on the chemosensitivity of human breast cancer cell. Methods MCF-7 cells were cultured as monolayer, incubated with cytokine IL-1β. The pro-duction of NO was detected by NO assay. The expression of iNOS protein was measured by Western blotting. Establishing control group and experimental group, the chemosensitivity of MCF-7 cells incubated by L-NMMA and L-Arg to ADM and 5-Fu was studied by MTT assay. Results There was a positive correlation of dose-dependence between NO production and IL-1β concentration. MCF-7 cells expressed plenty of iNOS by induetion of IL-1β. There was no significant difference on iNOS whether L-NMMA and L-Arg existed or not. Incubating MCF-7 cells with 0. 5 μmol/L or 1 μmol/L ADM, the survival rate of experiment group was remarkablely decreased(P < 0.05) ; L-NMMA significantly increased survival rate of experiment group(P < O. 05) ; L-Arg decreased survival rate of experiment group(P < 0.05). Conclusion The induction of IL-113 in MCF-7 cells can increase the production of endogenous NO, which increases MCF-7 cells' sensitivity to chemotherapy.
10.Analysis of long tubular bone fracture healing in 37 patients with osteofluorosis
Wen-zhe, YIN ; Jia-min, WANG ; Yu-ge, ZHAO
Chinese Journal of Endemiology 2008;27(4):455-457
Objective To study the correlations between bone fracture types and healing time in patients with osteofluorosis. Methods Thirty-seven patients with osteefluorosis and long tubular bone fracture were diagnosed in accordance with radiogram retrospectively. The fractures were divided into two groups: sclerotic and osteoporotic. Twenty four fractured patients with non osteofluorosis were included in the study as controls. All of the patients had operation(open reduction and nickelclad internal fixation). Fracture healing in patients with sclerotic and osteoporotic groups was compared with the control group after operation. Results There were notable differenees(F=4.30,P< 0.05) in term of fracture healing time among the three groups [sclerotic group:(18.4±5.3)weeks; osteoporotic group: (24.5±5.1)weeks; control group: (17.6±3.8)weeks]. Notably, there were significant differences between the osteoporotic and control groups(q=2.34,P<0.05), and between sclerotic and osteoporotic gronps(q=2.51, P<0.05). The healing time of the osteoporotic group was longer than that of sclerotic group. The constituent ratios of fracture healing in sclerotic, osteoporotic and control groups were 73.1% (19/26) ,54.5% (6/11),75.0% (18/24) respectively, and the differences among the three groups were statistically significant(X2=3.67,P<0.05). The healing rate of the osteoporotic group was lower than that of sclerotic and control groups(X2=3.12, 3.36, all P< 0.05). The constituent ratios of healing in the sclerotic, osteoporotic and control groups were 26.9% (7/26),45.5% (5/11),25.0%(6/24), respectively, and there differences among the three groups were statistically significant (X2=4.07 ,P<0.05). The delayed healing rate of the osteoperotic group was higher than those of the sclerotic and control groups(X2= 3.87,3.95, all P<0.05). Conclusions Fracture healing time of osteoporotic osteofluorosis after fracture is longer than normal, and the cause might be the loss of bone mass.