Objective To explore clinical effect and safety of amiodarone combined with bisoprolol in patients with congestive heart failure and ventricular arrhythmia. Methods Selected 100 cases with congestive heart failure and ventricular arrhythmia in our hospital from January 2011 to April 2017 as research objectives and divided them into two groups randomly with 50 cases in each group. Provided amiodarone to control group and provided amiodarone combined with bisoprolol to observation group. Compared two groups' arrhythmia and cardiac function, heart rate, ejection fraction, QT time (QTc) corrected for heart rate before and after treatment as well as adverse events. Results Observation group's effective rate of arrhythmia treatment and the effective rate of cardiac function were 94.00％, 96.00％, those were significant higher than control group's 70.00％, 78.00％ (P<0.05). Two groups' heart rate and ejection fraction score after 3 month treatment were significant higher than those before treatment (P<0.05), and observation group's improvement was more significant (P<0.05). Observation group's QTc was significantly prolonged after 3 months of treatment (P<0.05), but control group's QTc did not change significantly. There was no significant difference in the incidence of adverse reactions between the two groups. Results Amiodarone combined with bisoprolol has significant clinical effect on patients with congestive heart failure and ventricular arrhythmia. It is safe and worthy to be promoted clinically.

OBJECTIVETo investigate whether bortezomib can enhance the sensitivity of human prostate cancer (PCa) cells to natural killer (NK) cell-mediated cytotoxicity, and whether it produces the same effect on different PCa cell lines.

METHODSWe treated androgen-dependent PCa LNCaP cells and androgen-independent PCa DU145 cells with bortezomib at the concentrations of 0, 5, 10, 15, 20 and 25 nmol/L for 24, 48 and 72 hours, and then detected the proliferation and apoptosis of the tumor cells by CCK-8 and Annexin V/PI, respectively.

RESULTSThe proliferation rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (82.79 +/-2.04)%, (73.59+/- 2.95)% and (74.16+/- 6. 16)% at 48 hours and (71.24+/- 5.30)%, (51.20+/- 2.91)% and (38.02+/- 2.67)% at 72 hours, and those of the LNCaP cells were (77.04+/- 7.74)% , (42.61 +/- 6.62)% and (23.85 +/-6.04)% at 48 hours and (36.45 +/-7.02)%, (14.94 +/-5.76)% and (11.65 +/-5. 87)% at 72 hours, both significantly inhibited as compared with the control group (P <0.05). At 24 hours, the apoptosis rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (14.41 +/- 1.32)% , (16.13 +/- 1.55)% and (14.48 +/- 1.42)% , and those of the LNCaP cells treated with 20 and 25 nmol/L bortezomib were (12.77 +/- 1.28)% and (14. 84 +/- 1.65)% , significantly higher than those of the control group (P <0.05) , and the DU145 cells showed an even higher sensitivity to bortezomib than the LNCaP cells. Bortezomib failed to sensitize these two cell lines to NK cell-mediated cytotoxicity in short-term assay, while long-term assay manifested that the apoptosis rates of DU145 and LNCaP cells after treated with 20 nmol/L bortezomib + NK cells were (41.83 +/- 5.06)% and (30.31 +/- 3.62)% , respectively, significantly higher

CONCLUSIONBortezomib enhances the sensitivity of than those after treated with either bortezomib or NK cells alone (P <0.05). PCa cells to NK cell-mediated cytotoxicity and adds to the effect of current cancer therapies, and it is more efficacious for androgen-independent prostate cancer.

Apoptosis ; drug effects ; Boronic Acids ; pharmacology ; Bortezomib ; Cell Line, Tumor ; Cytotoxicity, Immunologic ; drug effects ; Humans ; Killer Cells, Natural ; drug effects ; Male ; Prostatic Neoplasms ; pathology ; Pyrazines ; pharmacology

Objective To investigate the relationship between ambulatory arterial stiffness index (AASI) derived from blood pressure monitoring and early signs of renal damage in patients with primary hypertension． Methods 74 primary hypertensive outpatients were divided into two groups according to their AASI values: normal AASI group (AASI≤0.51, n=40) and high AASI group (AASI>0.51, n=32). The urinary micro-albumin, glomerular filtration rates (GFR) were measured and compared. The relationship between AASI and micro-albumin, GFR were tested with Pearson correlation and multiple Logistic regression. Results Compared with those in the normal AASI group, the patients in high AASI group showed a higher level of urinary microalbumin (P<0.05) and a reduction in GFR (P<0.01). AASI was positively correlated with urinary microalbumin （r=0.32, P<0.001）, and negatively correlated with GFR （r=0.44, P<0.001）. After adjusting the potentially confounding variables, the odd ratio (OR) of AASI to renal damage was 2.18 （P=0.008，95%CI：1.76～4.34）. Conclusion The increase of AASI is associated with early signs of renal damage in patients with primary hypertension.

Diagnostic Techniques and Procedures ; standards ; Humans ; Infant, Newborn ; Jaundice, Neonatal ; diagnosis ; Reference Standards

Objective To evaluate the effectiveness of arthroscopically assisted percutaneous screw fixation for treating patella fractures.Methods A total of 14 cases of patella fractures were treated by percutaneous reduction and internal fixation with screws under arthroscope from November 2001 to June 2004 in this hospital.The fracture was reduced by closed manipulation or percutaneous leverage force using the Kirschner wire.Then the patella was temporarily fixed by using a large-sized towel clamp or Kirschner wires.Under the guidance of knee arthroscopy or C-arm fluoroscopy,a micro-incision was made at the site of screw placement,the pilot holes were drilled at a proper depth,and the thread was configured.Two titanium screws or absorbable screws were inserted parallelly.Results Follow-up checkups for 6~18 months(mean,10 months) in 13 cases showed a satisfactory recovery of knee functions.According to the Xu Shaoding's standard,excellent effects were obtained in 11 cases(84.6%) and good effects,2 cases(15.4%).Conclusions Treatment of patellar fractures by percutaneous screw fixation under arthroscope offers advantages of minimal invasion,accurate reduction,reliable fixation,and excellent recovery of joint functions.

Objective To explore the pre-requisite "highlight" of information system in a new outpatient building and its significance on outpatient information construction.Methods The importance of new outpatient information system for improving the treatment of the environment and optimizing the outpatient treatment processes were analyzed from information dissemination system,self-service system and intelligent queuing system.Results The systemic design plan and actual role of the three systems were given by combining actual situation of the new outpatient building construction.Conclusion Having greatly improving for the outpatient information construction and the new outpatient building will have a positive impact on hospital management model,medical model and informationation construction.

Objective To investigate the function and possible mechanism of acupuncturing “Zusanli” point on the liver cell membrane of rat with chronic alcohol liver injury. Methods Chronic alcoholic liver damage rat model was established by 60% ethanal intragastric infusion for 60 d. Total phosphatide, total cholesterol and phosphatide component were determined. Result Compare with the normal recovery group, acupuncture “Zusanli” point can increase the content of PC and PE, decrease the content of Chol and SM of liver cell, and reduce the membrane aging index C/P, SM /PC. Conclusion Acupuncture “Zusanli” point has the function of protecting the liver cell membranes and improving the liver cell membrane’s fluidity of chronic alcohol liver injury rat.

Objective To research the relationship among the disc protrusion on MRI, sex, age, course and the clinical types. Methods MRI was performed for 102 patients. The disc protrusion was divided into three types. According to clinical types, the patients were divided into four types. Statistically, age, course, clinical type and protrusion on MRI were analyzed by SPSS13.0. Results The age was not related with the clinical types and disc protrusion on MRI. There were obvious relations between clinical types and course. There were obvious relations between clinical types and disc protrusion. Conclusion The clinical types are very important on diagnosis of disease stages and choice of therapy in treating lumbar disc protrusion.