To prepare decalcified bone matrix particles(DBMP)with adriamycin(ADM) impregnated bone cement(BC) drug delivery system(DDS), its drug delivery characteristics were studied and analysed in vivo. DBMP were made according to the methods of Urist et al. ADM was putting into DBMP by vacuum absorption and freeze drying techniques. Then the DBMP with ADM were impregnated into BC in 1∶1 to form DDS. The DDS was implanted into the greater trochanter of femur in rabbits and the release test was done in vivo. The DDS could gradually release ADM for over 12 weeks in local bone tissues, maintained stable release for 4 weeks. The maximum ADM in local bone tissues was 29 times higher than that of the same intrarenous dose, while the maximum ADM in plasma was only 1/10 of the same intravenous dose. The DDS has a good sustained release function, and can meet the needs of local chemotherapy for limb salvage treament of bone tumor.

Animals ; Heparin ; pharmacology ; Humans ; Ligands ; Lymphatic Metastasis ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Oligosaccharides ; metabolism ; Selectins ; metabolism ; physiology ; Signal Transduction

Objective To evaluate the effect of calcitonic gene related peptide(CGRP) and endothelin(ET) on acute and delayed cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH).Methods The SAH model in rabbit were established by single injection of autologous arterial blood(0.5 ml/kg) to cisterna magna on percutaneous suboccpital route. Transcranial Dopper(TCD) was performed to detect the spastic of rabbit basilar artery(BA). The CGRP and ET concentrations in cerebrospinal fluid were examinated quntitatively by radioimmunologlc techniques at 30 minutes, 3 days, and 5 days after SAH.Results The results of TCD showed that rabbit basilar artery was spastic at 30 minutes, 3 days and 5 days after SAH. At 30 minutes after SAH, CGRP concentration significantly increased upto about 18 fold( P

Objective To evaluate and compare open and retroperitoneal laparoscopic adrenalectomy.Methods Clinical data of 30 cases undergoing open adrenalectomy and 119 cases undergoing retroperitoneal laparoscopic surgery were retrospectively analyzed. The 2 groups were compared in terms of these aspects: operation duration, intraoperative blood loss, postoperative analgesic use, time to remove drainage tube, time to resume eat, postoperative hospitalization time, complications, and tumor recurrence. In open surgery group, tumor diameter was between 0.5 cm -10.8 cm, 4.57 cm in average. 18 tumors were located on the left side and 12 tumors on the right side. In laparoscopic group, tumor diameter was between 0.8 cm -14.5 cm, 2.78 cm in average. 59tumors were located on the left side and 60 tumors on the right side. Patients in open surgery group were followed up for 6-72 months and retroperitoneal laparoscopic group 4-20 months. Results Open adrenalectomy were successful in all the 30 cases. 12 cases in laparoscopic surgery group converted to open surgery among whom 8 cases were due to poor visibility, 1 case due to renal artery injury, 1 case due to large tumor size, 1 case because of diaphragmatic injury and 1 case because of pleural injury. Laparoscopic surgery was superior to open surgery in terms of operation duration, intraoperative blood loss, postoperative analgesic use, time to start food taking, time to remove drainage tube, and postoperative hospitalization time. The difference had statistical significance (P ＜0.05 ). Conclusion Retroperitoneal laparoscopic adrenalectomy has advantages such as less trauma, less blood loss, and shorter recovery time, which make this procedure the modern golden standard for treatment of benign adrenal neoplasm.

Objective:Comparative study on clinical manifestation of the lumbar disc herniation(LDH) patients with Japanese Orthopedic Association Scale (JOA) before and after treated by massage with Chinese medicine. Methods:Clinic information of 110 LDH patients was selected according to settled criteria. Experiment group: 70 patients were treated by manipulation and Biqi Capsule. Control group: 40 patients were treated by manipulation and Fengshi Gutong Capsule. JOA and SAS 9.0 were used. Results: There were significant changes of JOA score before and after treatment in experiment group (t=20.68767,P

Objective:To evaluate the therapeutic effects of capsule of Biqi Capsule and VitB on lubar intervertebral disc protrusion(LIDP).Methods:78 Patients with LIDP were divided randomly into 2 groups:treatment and control group.39 Patients in treatment group were treated with Biqi Capsule and FENG's bone-setting manipulation,39 patients in control group were treated with vitamine B and FENG's bone-setting manipulation.All patients were followed up for 1 month after treatment for 2 weeks and therapeutic effects were evaluated.Results:In treatment group,24 patients were cured,6 patients had remarkable effects,5 effective,4 ineffective.In control group,the data were 16,8,8,7 correspondly.The therapeutic effects in treatment group were obviously better than those of control group(P

Objective To discuss the teaching outcomes of problem-based learning method in classroom teaching of TCM pharmaceutics.Methods Fifty students majoring in 2009 science of Chinese materia medica were taught by using traditional teaching method in teaching of TCM pharmaceutics, and 56 students majoring in 2009 TCM pharmaceutics by using of problem-based learning method. This study evaluated students’ adaption to the two teaching methods through score analysis and questionnaire of the two classes.Results Students taught by problem-based learning method generally believed that this teaching method can stimulate their learning interest, improve self-study ability, thinking ability, creativity, and language competence, and enhance team spirit.Conclusion Problem-based learning method shows better teaching outcomes than traditional teaching method. It can guide students to look for ways to tackle problems in the course of solving problems, and cultivate all-round ability and comprehensive quality of students.

Objective:To study the effects of smokeless tobacco extract(ST)on the proliferation and the heat shock protein 70 (HSP70)expression of human periodontal ligament cells(hPDLCs).Methods:hPDLCs were cultured in vitro and identified by im-munohistochemistry(IHC).The cells were stimulated with ST at 0.01 6 -50 g/L respectively for 24 h,the proliferation was examine by MTT assay,HSP70 expression was detected by immunehistochemical staining and Western Blot.Results:ST inhibited the prolifer-ation and increased HSP70 expression in cytoplasm and nucleus at 0.4 -50 g/L dose dependantly.Conclusion:ST may inhibit the proliferation and increase HSP70 expression of hPDLCs in a dose depandant manner.

Objective To establish the quality control method for Sanhuang Suppository. Methods Coptidis rhizoma, Scutellariae radix and Phellodendri chinensis cortex in Sanhuang Suppository were identified by TLC. The contents of baicalin and berberine hydrocholride were determined by HPLC which was performed on an Agilent Zorbaxsb C18 column (4.6 mm×250 mm, 0.45 μm) with a mobile phase of methanol-water-phosphoric acid (47∶53∶0.2), with flow rate of 1.0 mL/min. The wavelength of detector was set at 280 nm for baicalin and 365 nm for berberine hydrochloride, with column temperature of 30℃.Results Chromatographic characteristics of qualitative identification were evident. The linear range of baicalin was 0.248-2.48 μg (r=0.999 9) and the average recovery rate was 99.77% (RSD=1.05%). The linear range of berberine hydrocholride was 0.336-1.68 μg (r=0.999 9) and the average recovery rate was 97.74% (RSD=1.48%).Conclusion The method of qualitative and quantitative analysis is accurate, feasible and suitable for effective quality control of Sanhuang Suppository.

Background and purpose: High-dose chemotherapy followed by autologous stem cell transplantation (auto-HSCT) is considered as the ifrst line treatment for patients with relapse/refractory lymphoma after conventional chemotherapy. However, most of these patients still relapse the second time. The purpose of this study was to analyze the efifcacy of the consolidation chemotherapy after autologous stem cell transplantation (HSCT) refractory/relapse lymphoma in high risk. Methods:A total of 38 patients with relapsed/refractory lymphoma including Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL) were included, who were underwent auto-HSCT in our transplan-tation department from Jan. 2010 to Dec. 2013. In treatment group, 19 patients received 2 courses of consolidation che-motherapy after auto-HSCT every 2 to 3 months, with the regimen of mini-BEAM or modiifed mini-CBV. Another 19 patients had no chemotherapy after auto-HSCT as control group. Results:The median follow-up duration was 17.2 and 7.5 months in the treatment and control group respectively. The follow-up data demonstrated prolonged progression-free survival (PFS) in the treatment group than the control group [24.7 months vs 7.8 months, P=0.029 under intend-to-treat analysis ITT;24.7 months vs 5.2 months, P=0.01 under per protocol analysis(pp)]. There is also a trend of improved overall survival (OS) in the treatment group (P=0.055, ITT). Conclusion:Consolidation chemotherapy after auto-HSCT for refractory/relapsed lymphoma patients delay the relapse and tend to improve the overall relapse rate.