Stem cells are a lineage with capacities of self-renewal and multipotential differentiation. They are exactly regulated by microenvironment where they live. With the understanding of stem cells biology, researchers noticed that stem cells have many similar biology characters with tumor cells, and thought that tumors were likely derived from stem cells, and gave a concept that there were tumor stem cells in tumor tissues.

The chemical constituents of Vaccinium bracteatum were studied by means of macroporous resin, ODS column chromatography and preparative HPLC. Eleven compounds were isolated from this plant. By using ESI-MS and NMR, the structures of the eleven compounds were determined as 10-O-trans-p-coumaroyl-6alpha-hydroxyl-dihydromonotropein (1), 10-O-cis-p-coumaroyl -6alpha-hydroxyl-dihydromonotropein (2), vaccinoside (3), 10-O-cis-p-coumaroyl monotropein (4), isolariciresinol-9-O-beta-D-xyloside (5), tectoridin (6), vicenin-3 (7), quercetin-3-O-alpha-L-rhamnoside (8), quercetin-3-O-alpha-L-arabinopyranoside (9), quercetin-3-O-beta-D-galactopyranoside (10), and quercetin-3-O-beta-D-glucuronide (11), respectively. Compounds 1 and 2 are new, and compounds 4, 6 and 7 are isolated from the genus Vaccinium for the first time.
Drugs, Chinese Herbal ; chemistry ; Molecular Structure ; Spectrometry, Mass, Electrospray Ionization ; Vaccinium ; chemistry

CD4 Antigens ; immunology ; Humans ; Interleukin-2 Receptor alpha Subunit ; immunology ; T-Lymphocyte Subsets ; immunology ; physiology ; T-Lymphocytes, Regulatory ; physiology

Objective: To analyze the influencing factors in thrombin titration for the determination of anticoagulant activity of Whitmania Pigra Whitman. Methods: The white porcelain plates were used as the titration carriers instead of tubes in the titration ( called white porcelain method for short) . The effect of different carriers, interval time of titration and thrombin concentration on the results of anticoagulant activity test was studied. Results:Under the same conditions, the anticoagulant activity was more accurate and stable using white porcelain method. Using white porcelain method with 20 u·ml-1 or 10 u·ml-1 as the thrombin concentration and titrating 5μl each time, once every minute, the thrombin consumption volume was linear with the sample concentration within the range of 0. 125-0. 333 g·ml-1(r20 =0. 961 and r10 =0. 992), and the anticoagulant activity respectively was (33. 08 ± 2. 64) and (31. 24 ±1.32) u·g-1(RSD20 =8.0% and RSD10 =4.2%). As for a certain sample concentration (0.333 g·ml-1), the theoretical error of determination was not more than 10% and 5%. Conclusion:The improved white porcelain method is more suitable for determining anticoagulant activity of Whitmania Pigra Whitman with more stable results and accurate end point states than tube method. Under the conditions of 10 u·ml-1 thrombin concentration, titrating 5μl each time, once every minute, the linearity, accuracy and precision are all promising.

Objective To assess the effect of QRS duration (QRSd) and cardiac function during right ventricular apex(RVA) pacing,right ventricular outflow tract (RVOT) pacing and right ventricular bifocal (RV-Bi) pacing. Methods Eight patients underwent RVA pacing,RVOT pacing and RV-Bi pacing during pacemaker implantation operation.The ejection fraction (EF),stroke index (SV),cardiac output(CO),QRS QRSd,QRS axis (QRSa) were measured after each pacing at the same pacing frequency. Results Compared with RVA pacing,the EF,SV and CO increased during RVOT pacing and RV-Bi pacing.The cardiac function of RV-Bi pacing was significantly increased (P

Objective To investigate the characteristic of the cisplatin-induced damage of hair cells in cochlear organotypic cultures. Methods Three to four-day-old (P3～4) C57 mice were used for this study. The cochlear basilar membrane was cultured for 6~8 hours and then treated with different doses of cisplatin(0?mol/L, 10?mol/L, 50?mol/L, 100?mol/L, 400?mol/L, 1000?mol/L,7 cochlears for 0-100?mol/L group, 6 cochlears for the rest dosage, control group used the dose of 0?mol/L)for 48 hours.TRITC-phalloidin and DAPI were used for stain. The entire cochlear hair cells were observed and counted under Nicon fluorescent microscope. Data was analyzed by LS for hair cells loss graph and SAS 8.0 for linear regression analysis. Results Hair cells loss had rarely been seen in the control group of cochlear tissue under the culture condition. How ever, in cisplatin treated groups, the hair cells loss increased from 14.5% to 78.4% with the concentration of cisplatin increased in the range of 10?mol/L-100?mol/L, while decreased from 48.8% down to 8.77% with the dose getting further higher to 400?mol/L and 1000?mol/L. Apoptosis was confirmed by the DAPI stain in the hair cells loss area. The hair cell loss shows no difference from the apex to the basal turn of the basilar membrane. Conclusion Cisplatin-induced hair cell death shows characteristic damage that it is dose-dependent in a certain range of dosage, and it decreases when the cisplatin dose higher than the one caus maximal damage under the condition of cochlear organotypic cutures. Apoptosis happens evenly in inner and outer hair cells in all turns of basilar membrane.

Adult ; Aneurysm, Dissecting ; complications ; Aortic Aneurysm ; complications ; Humans ; Lower Extremity ; physiopathology ; Male

BACKGROUND: Cell apoptosis and ventricle reconstitution following myocardial infarction are of mutual cause-effect, and they cause vicious cycle. How to reduce the apoptosis events following myocardial infarction is one of keys to saving heart function.OBJECTIVE: To observe the effect of umbilical cord blood mesenchymal stem cell (UCBSMC) transplantation on remaining myocardial tissue of dogs with acute myocardial infarction.DESIGN: A randomized controlled observation.SETTING: Department of Cardiothoracic Surgery, Xinhua Hospital.MATERIALS: This study was carried out in the Central Laboratory of Xinhua Hospital from October 2005 to May 2007.Thirty-six adult hybrid dogs, male and female in half, were provided by the Animal Experimental Center of Xinhua Hospital.METHODS: Thirty-six dogs were divided into cell transplantation group and control group, with 18 dogs in each according to table of random digit. Mesenchymal stem cells were isolated from the umbilical cord blood of full-term pregnant hybrid dogs, cultured and amplified. Then, they were labeled with Laz gene, in vitro induced with 5-azacytidine, and transplanted into the dogs with acute myocardial infarction in the cell transplantation group. Rats in the control group were injected with the same amount of normal saline. Each dog was euthanized by anesthesia for harvesting myocardial specimen 1,4 and 8 weeks after transplantation.MAIN OUTCOME MEASURES: ① Remaining and apoptosis index detected by TUNEL method. ② Myocardial cell volume and histomorphology detected by confocal microscopy. ③ Histological change of myocardial collagen network detected by haematoxylin-basic fuchsin-picric acid staining.RESULTS: Thirty-six involved experimental dogs all entered the stage of final analysis. ①The apoptosis index in the cell transplantation group was significantly lower than that in the control group 1, 4 and 8 weeks after cell transplantation (P ＜0.05). ② Myocardial cell volume in the cell transplantation group 1, 4 and 8 weeks after cell transplantation was significantly larger than that in the control group (P ＜ 0.05). ③ Collagen fiber in the myocardial tissue of dogs in the cell transplantation group was arranged in order and regularly, and in contrast that in the control group was not, and fibers in the control group fused partially.CONCLUSION: UCBSMC transplantation reduces the apoptosis of myocardial cells, promotes the hypertrophy of remaining myocardial cells, regulates myocardial collagen network and improves heart function.

Objective To identify the typical imaging features of steatocystoma multiplex on mammography.Methods Mammographic findings in 9 patients with clinical and pathological diagnosis of steatocystoma multiplex were analyzed along with a review of the current literature.Results Four of the nine patients with steatocystoma multiplex had a positive family history.Nine patients showed multiple,round,thin-walled fatty,radiolucent nodules with well-defined margin.These nodules are located in the superficial layer of the axilla bilaterally.They are also seen on the skin of breast(7 cases),the anterior chest wall (4 cases) and the upper arm (3 cases).Conclusion Recognition of the characteristic mammographic features of Steatocystoma multiplex is important in the management of these patients.

Objective:To study the change rule of rats'pulse waves after kidney resection.Methods:To collect the pulse waves of femoral artery in each side of rats in Drugged state.Then do one-side kidney resection operation,after fully recover from it,collect the pulse waves again.To average the pulse waves in the same cycle length and then do the LSQ regression by 6 harmonics which are correspond with the cycle lengths,analyze the phase and the amplitude of vibration of fundamental wave and harmonics by statistical method.Results:The amplitude of vibration of the 3rd harmonics of pulse waves of femoral artery in the same side with operation and the amplitude of vibration of the 1st to 5th harmonics of pulse waves of femoral artery in the other side with operation has reinforced, the phase of the 1st and 2nd harmonics also have changed regularly.Conclusion:There're some regular changes in the pulse waves of rats after one-side kidney resection operation,which shows that the pulse waves of artery on the body surface can reflect the splanchnic changes.This research can offer some scientific foundation to the pulse-diagnosis in TCM.