1.Diagnosis of spinal nerves location combined with acupoints selection according to Hand-Sanyang meridian for 60 cases of cervical spondylosis of nerve root type.
Jin-niu LI ; Yu-ling LENG ; Ru SUN
Chinese Acupuncture & Moxibustion 2014;34(9):887-888
Acupuncture Points
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Adult
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Female
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Humans
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Male
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Meridians
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Radiculopathy
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diagnosis
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therapy
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Spondylosis
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diagnosis
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therapy
2.Effect of lacrimal duct laser with lacrimal drainage tubes and stents for complexity dacryagogatresia
Yu-Ru, LI ; Xiang-Fu, CHENG ; Xiao-Rui, LIU
International Eye Science 2016;16(7):1378-1379
AIM: To explore the effect of lacrimal duct laser with lacrimal drainage tubes and stents implantation for complexity dacryagogatresia.
METHODS: There were 65 patients ( 82 eyes ) with compound tears nasolacrimal duct obstruction who received lacrimal drainage tubes and stents implantation after laser. The lacrimal duct stents were removed through nasal cavity after 1mo. Lacrimal drainage tubes were removed after 3-6mo. Follow-up periods were 6mo to 1a.
RESULTS: In the 65 patients (82 eyes), 71 eyes were cured, 5 eyes improved, the efficient rate was 93%; there were 6 eyes (7%) with epiphora.
CONCLUSION: Lacrimal duct laser with lacrimal drainage tubes and stents implantation was efficient for complexity dacryagogatresia.
3.Changes of Amplitude of Ion Channels Currents in Developing Hippocampal Neurons
zhen-biao, LI ; xi-ru, WU ; yu-wu, JIANG
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To study the changes of the amplitude of ion channels currents in developing hippocampal neurons.Methods Using whole-cell patch-clamp techniques in cultured hippocampal neurons whose cultured day were 6 d and 16 d,respectively,changes of the amplitude of ion channels currents in developing hippocampal neurons were explored.Results Compared with the hippocampal neuron whose cultured day was 6 d,there were no statistical differences of the amplitude of voltage dependent sodium currents of hippocampal neuron whose cultured day was 16 d.The amplitude of voltage dependent potassium currents of hippocampal neuron whose cultured day was 16 d were significantly increased(P
4.Changes of N-Methyl-D-Aspartate-Receptor-Channels Current in Developing Hippocampal Neurons after Hypoxia and Effect of Adenosine Intervention
zhen-biao, LI ; xi-ru, WU ; yu-wu, JIANG
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To study the changes of N-methyl-D-aspartate(NMDA)-receptor-channels current in developing hippocampal neurones during hypoxia and effect of adenosine intervention.Methods Whole-cell patch-clamp techniques cultured hippocampal neurons whose cultured day were 6 days and 16 days respectively,the amplitude of the NMDA-receptor-channels currents of hippocampal neuron were determined.And the effect of hypoxia on the NMDA-receptor-channels current,and adenosine regulatory mechanisms in cultured hippocampal neurons were explored.Results During hypoxia,compared with control group,the amplitude of the NMDA-receptorchannels currents of hippocampal neuron whose cultured day was 6 days were significantly increased(P
6.Progress on Hypoxic-ischemic Brain Damage Associated with CCR2 and CCL2.
Yu-jia LUO ; Ru-bo LI ; Shi-yu MA ; Meng-yan LÜ
Journal of Forensic Medicine 2016;32(1):54-57
Hypoxic-ischemic brain damage (HIBD) is referred to a common type of cerebral damage, which is caused by injury, leading to shallow bleeding in the cortex with intact cerebral pia mater. In recent years, studies show that a various kinds of immune cells and immune cellular factors are involved in the occurrence of HIBD. CC chemokine receptor 2 (CCR2) is a representative of CC chemokine receptor, and is widely distributed in cerebral neuron, astrocyte, and microglial cells, and is the main chemo-tactic factor receptor in brain tissue. CC chemokine ligand 2 (CCL2) is a kind of basophilic protein and the ligand of CCR2, and plays an important role in inflammation. In order to provide evidence for correlational studies in HIBD, this review will introduce the biological characteristics of CCR2 and CCL2, and illustrate the relationship between the immunoreactivity and HIBD.
Animals
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Brain Injuries/pathology*
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Cerebral Cortex/physiopathology*
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Chemokine CCL2/metabolism*
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Chemokines, CC/metabolism*
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Hypoxia-Ischemia, Brain/metabolism*
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Macrophage Inflammatory Proteins/metabolism*
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RNA, Messenger/metabolism*
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Rats
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Rats, Sprague-Dawley
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Receptors, CCR2/metabolism*
7.The expression and the function of miR-155 on rheumatoid arthritis synovial fibroblasts
Ping YU ; Li LONG ; Shiyao WANG ; Ru LI ; Xiaoping ZHANG ; Yanying LIU ; Liufu CUI ; Zhanguo LI
Chinese Journal of Rheumatology 2010;14(7):460-463
Objective To screen for the miR-155 expression in synovial fibroblasts of rheumatoid arthritis (RASFs) and osteoarthritis (OASFs) and to evaluate the function of miR-155 on RASFs and its possible target mRNAs. Methods The expression levels of miR-155 in RASFs and OASFs were detected by real-time PCR. MiR-155 mimic and miR-155 inhibitor, as well as scrambled control were transfected into cultured RASFs by Lipofectamine 2000. Forty-eight hours later, MMP-3 levels in the cell culture supernatant were detected by ELISA and fibroblast proliferation was assayed by 3H -TdR incorporation test. Fibroblast invasive ability was tested by transwell system. IKBKE which previously identified as actual target of miR-155 was examined by real-time PCR. Comparisons between groups were performed with t test or one-way ANOVA analysis. Results It was shown that miR-155 was up-regulated in RASFs (1.79 ±1.94) and it was higher than that in OASFst (0.11±0.17), P<0.05]. Up-regulation of miR-155 could decrease MMP-3 levels (P<0.05). The proliferation and invasion of RASFs transfected with miR-155 were both evidently suppressed (P<0.05), while reducing the endogenous miR-155 could significantly enhance RASF proliferation (P<0.05). The expression of IKBKE of RASFs transfected with miR-155 was obviously down-regulated compared to those transfected with the scrambled control (P<0.05). Conclusion miR-155 is up-regulated in RASFs which may be a protective factor against the inflammatory effect, at least partially by attenuating the expression of IKBKK.
8.Post-transplant lymphoproliferative disorders: a report of two cases.
Zhao-ming WANG ; Li-jun WANG ; Xin-ru YU ; Li-ming XU
Chinese Journal of Pathology 2006;35(10):639-640
Antigens, CD20
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analysis
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Bone Marrow Transplantation
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adverse effects
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CD79 Antigens
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analysis
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Female
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Humans
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Immunohistochemistry
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Liver Transplantation
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adverse effects
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Lymphoma, Large B-Cell, Diffuse
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etiology
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metabolism
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pathology
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Lymphoproliferative Disorders
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etiology
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metabolism
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pathology
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Male
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Middle Aged
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Postoperative Complications
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etiology
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metabolism
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pathology
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Young Adult
9.Altered expression of miR-155 in peripheral blood mononuclear cells of rheumatoid arthritis patients
Ping YU ; Li LONG ; Jinxia SHI ; Ru LI ; Xu LIU ; Liufu CUI ; Xuewu ZHANG
Chinese Journal of Rheumatology 2015;19(3):148-151
Objective ① To Screen for the miRNAs differently expressed in the peripheral blood mono-nuclear cells (PBMCs) of rheumatoid arthritis (RA) by microarray experiments.② To further evaluate the expression of miR-155 in PBMCs of RA.③ To determine the relevance between the expression of miR-155 and clinical as well as laboratory features.④ To test whether inflammatory mediators can induce miR-155 expression in PBMCs of RA.Methods ① Total RNA was isolated from peripheral blood mononuclear cells obtained from 5 patients of RA and 5 normal controls.Expression profiling of miRNAs was performed in a microarray analysis.② MiR-155 was identified for further study by stem-loop real-time RT-PCR based on SYBR-Green.PBMC from 26 patients of RA and 23 normal controls were collected.③ Association between miR-155 and the clinical and laboratory features of RA was evaluated.④Induction of miR-155 following stimulation with TNF-α, IFN-γ and LPS of cultures of RA PBMCs was examined by real-time RT-PCR.Statistical analysis was done with student's t test, paired t test, and ANOVA, Spearman correlation.Results ① Expression profiling of miRNAs revealed significant differential expression of 14 miRNAs, of which signal intensity changed over two times.MiR-155 was up-regulated in PBMCs of RA than in normal controls (t=9.218, P=0.001).② The expression level of miR-155 had a positive correlation with serum CRP level (r=0.57, P=0.002).③ Expression of miR-155 was markedly up-regulated in PBMCs of RA after stimulated with TNF-α,IFN-γ and LPS, especially with TNF-α.Conclusions The expression of miR-155 is induced by stimulating with TNF-α, IFN-γ and LPS.MiR-155 may be a regulator in RA pathogenesis.Further studies are required to elucidate the function of miR-155.
10.Association studies of G352A polymorphism of dopamine transporter gene in Han Chinese attention deficit hyperactivity disorder patients
Qiu-Jin QIAN ; Yu-Feng WANG ; Ru-Lun ZHOU ; Li YANG ; Jun LI ;
Journal of Peking University(Health Sciences) 2003;0(06):-
Objective:To investigate association of the new polymorphism G352A in the dopaminetransporter gene(DAT1)exon 15 with attention deficit hyperactivity disorder(ADHD)in Han Chinesechildren.Methods:The new mutant polymorphism G352A in the dopamine transporter gene(DAT1)ex-on 15 was found by the fluorescently-labeled dye-terminators assay.The study samples were comprised of337 ADHD children,207 unrelated controls and 201 integrated ADHD trios(included proband and bio-logical parents).Associations of polymorphisms with ADHD and its subtypes were examined by:(i)comparing cases and controls;and(ii)using family-based association study in transmission-disequilibri-um test(TDT).Results:The allele frequencies at the DAT1 G352A locus in the control samples were79.5% for 352G and 20.5% for 352A respectively.Association studies revealed no association betweenG352A in exon 15 of DAT1 and ADHD.But after a stratification by gender,there was possible associationbetween G352A and ADHD girls:the 352G allele had a tendency to be preferentially transmitted toADHD girls.Conclusion:There is no association between G352A,the new polymorphism,in exon 15 ofDAT1 and ADHD.The 352G allele has a tendency to be preferentially transmitted to ADHD girls,but thefindings require replication before drawing a definitive conclusion.