Brain Diseases ; etiology ; therapy ; Heat Stroke ; complications ; therapy ; Humans ; Hyperbaric Oxygenation ; Male ; Middle Aged ; Occupational Diseases ; complications ; therapy

2 cm). All patients received combination chemotherapy of abdomen and vein with CAP regime and TP regime after sections.Results:Total five year survival rates were 11% in epithelial ovarian carcinoma in stage Ⅲ. The 1-.3-.5-year survival rates of the cases which had less than 4 cycles of chemotherapy were lower than those ≥6 cycles, and the recurrent rates were higher than those ≥6 cycles. The 1-.3-.5-year survival rates of TP regime were higher than those of CAP regime,and the recurrent rates were lower than those CAP regime.Conclusions:There are many relationships between prognosis and residual tumor, nucmber of chemotherapy cycles or regime after resection,which influence results of recurrent ovarian cancer after repeated tumorectomy.

Objective To evaluate the effect on inflammatory mediators and mechanism of dynamic factors on lung injury in a dog model of acute respiratory distress syndrome (ARDS). Method The ARDS dog model was duplicated by instillation hydrochloric acid. The dogs were randomly (random number) divided into six groups: (1) normal control group (N group); (2) ARDS group (M group); (3) low VT (6 mL/kg) at respiratory rate 30, low inspiratory flow 6 mL/(kg·s). (4) large VT (20 mL/kg) at respiratory rate 30, high inspiratory flow 20 mL/kg·s.(5) large VT (20 mL/kg) at respiratory rate 15, high inspiratory flow 17 mL/(kg·s). (6) large VT (20 mL/kg) at respiratory rate 15, low inspiratory flow 10 mL/(kg·s). All the dogs were killed after 4 h ventilation. TNF-α、IL-8, p38 MAPK and NF-κB activity in the lung were measured. Results The expression of IL-8 protein in B and C groups was much higher than that of other groups ( P < 0.01) . There was no significant difference among M, A and D groups (P > 0.05). The gray scale ratio of B group was obviously higher than that of other groups (P < 0.01), except C group (P > 0.05). There was no significant changes among M, A and D groups in TNF-α protein contents. p38 MAPK value of positive staining of B group was the strongest, significantlyhigher than that of D group ( P < 0.01) .The expression of p38 MAPK in B and C groups was much higher than other groups (P <0.01). NF-κB activity in B group (33.56±2.85%) was significantly higher than that in A (10.35±0.6%)、D(7. 11 ± 0.47%)group, but there was no difference between B and C group (30.87 ± 1.16%). Conclusions Ventilation at high tidal volume, high inspiratory flow rate, high respiratory rate could activate p38 MAPK and increase the activity of NF-κB with the result of aggravating the release of inflammatory mediators. p38 MAPK and NF-κB activation are the major mechanisms in the development of VILI.

Objective:To investigate the vasodilatative roles and mechanisms of 17 ? estradiol(E 2) on rat thoracic aortas (TA). Methods:Rings cut from thoracic aortas of female rats were used by in vitro perfusion. The endothelium dependent and endothelium independent vasorelaxing effects of E 2 were measured. Furthermore, it was also observed whether the relaxing effects of E 2 were modulated by tamoxifen, N ? nitro L arginine methyl ester(L NAME),TEA, methylene blue(MB) or Methylene Blue(MB).Results:E 2 caused acute concentration dependent relaxation in TA with endothelium, but not significant without endothelium( P

Objective To assess the safety and efficacy of an olive oil-based lipid emulsion for parenteral nutrition in patients after esophagectomy.Methods In the randomized controlled trial,60 patients undergoing esophagectomy were divided into study group(n=30,received olive oil-based lipid emulsion)and control group [n=30,received medium-chain triglyceride/long-chain triglyceride(MCT/LCT)emulsion].The parenteral nutrition Was provided for 7-10 postoperative days.The nutritional formulas were equivalent in nitrogen,calorie,osmotic pressure,and fluid volume.Peripheral venous blood tests were performed before operation and on the first and eighth postoperative days.All the patients were evaluated by nutritional status(weight,body mass index,nutritional risk screening,etc.),safety profiles[full blood test,electrolytes,aspartate aminotransferase(AST),alanine amiotransferase(ALT),total bilirubin and direct bilirubin,blood urea nitrogen(BUN),creatinine,blood glucose,etc.],and efficacy indicators(hemoglobin,albumin,total protein,etc.).Results The albumin and total protein levels returned to the normal ranges in beth groups 8 days after operation,although both levels were significantly higher in study group(P=0.000).Also,the difference of total protein levels between the eighth and first postoperative days Was significantly higher in the study group(P=0.002).In addition,the AST and BUN readings returned to normal ranges 8 days after operation in the study group, which were significantly lower than those in control group (P = 0.025, P = 0.013).No serious adverse events were reported in both groups.Other nutritional parameters, renal and hepatic safety profiels, vital signs, and hematology showed no significant difference between two groups.Conclusions Olive oil-based lipid emulsion is a safe and efficient lipid emulsion for parenteral nutrition in patients undergoing esophagectomy.Compared with MCT/LCT, it has less effect on AST and BUN.

Epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions, such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu2+. EGCG was incubated with prostate cancer cells, LNCaP, pretreated with or without Cu2+.EGCG in F-12 medium was quantified using HPLC and the viability of cells was assessed by gel electrophoresis, flow cytometry,and electron microscope. The results of HPLC showed that EGCG degraded completely within 12 h in F-12 medium with or without Cu2+. Gel electrophoresis and flow cytometry did not detect apoptosis of LNCaP cells when they were incubated with EGCG. Electron microscopy examination revealed that EGCG-Cu2+ complex led to damage of cytoplasm membrane in LNCaP cells. It was speculated that not EGCG, but its oxide and complex with Cu2+, are the bioactive components responsible for its cytotoxicity to LNCaP prostate cancer cells.

Objective To observe the effects of 75 gram glucose oral tolerance test (75 g OGTT) and standard mixed meal test (SMMT) on insulin secretion function of the islets of Langerhans and plasma free fatty acid (FFA) in patients with type 2 diabetes mellitus.Methods Seventy-six patients with type 2 diabetes without using insulin and with no obvious complications were recruited for 75 g OGTT following overnight fasting on the first day and SMMT (bread 50 g,egg 50 g and milk 250 ml) on the 7th day.Blood specimens were collected from each patients before the tests and 30 min,60 min,120 min and 180 min after glucose or meal load to measure their levels of plasma glucose,serum insulin,C peptide,FFA and lipids (total cholesterol,triglyceride,high-density and low-density lipoprotein cholesterol).Results No difference in fasting plasma glucose,serum insulin,C peptide,FFA and lipids between 75 g OGTT and SMMT was found.Postprandial plasma glucose 30 min,60 min,120 min and 180 min after 75 g OGTT was significantly higher than that after SMMT,with (15.3?3.5) vs (9.9?3.4) mmol/L,(18.2?4.8) vs (12.8?4.0) mmol/L,(16.3?5.8) vs (12.2?4.9) mmol/L and (10.6?5.4) vs (9.5?4.5) mmol/L (F=28.1,P

Objective To investigate effect of comprehensive nutrition management on outcome of patients with celiac disease.Methods We retrospectively reviewed 17 consecutive patients with celiac disease diagnosed from January 2000 to December 2014 in Peking Union Medical College Hospital.Clinical features of these pa-tients were summarized.Nutrition management was implemented, involving gluten-free diet.Of these 17 pa-tients, 8 cases were followed up to assess their compliance and responses to nutrition management.Results The mean age of the recruited patients was (39.65 ±19.14) years.The male/female ratio was 9/8.All the patients were featured with chronic diarrhea of various severity, with 6 (35.29%) patients having steatorrhea ( positive sudan Ⅲstaining) and 7 (41.18%) patients showing positive occult blood in feces.Besides, low D-xylose ab-sorption (<1.2 g/5 h) was observed in 12 (70.59%) patients.The mean body mass index (BMI) at presen-tation was (16.57 ±3.05) kg/m2 .Twelve (70.59%) patients had malnutrition ( BMI<18.5 kg/m2 ) , inclu-ding 6 (35.29%) patients who had severe malnutrition ( BMI<15 kg/m2 ) .Hypoalbuminemia was present in 14 (82.35%) patients.Anemia was the most common extra-intestinal manifestation, found in 11 (64.71%) patients.Deficiency of iron, folate and vitamin B12 were confirmed in 6 ( 35.29%) , 3 ( 17.65%) , and 5 (29.41%) patients, respectively.Comprehensive nutrition management was administered to all the patients. Eight patients were followed up, with mean follow-up duration of (10.38 ±8.28) months (3-25 months) .Of the 8 patients, 7 were well compliant to nutrition management ( Likert scale scores 1-2) and had significant im-provement of diarrhea and abdominal distention, with body weight increasing from ( 44.83 ±9.77 ) kg before management to (47.17 ±9.30 ) kg ( P=0.681 ) and serum albumin elevating from ( 27.50 ±7.92 ) g/L at presentation to (34.20 ±3.27) g/L (P=0.102).Conclusion Patient education and comprehensive nutrition management with application of strict gluten-free diet, close follow-up, disease monitor, and guidance of dietary modification play a significant role in improving outcome and life quality of patients with celiac disease.

Epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions, such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu2+. EGCG was incubated with prostate cancer cells, LNCaP, pretreated with or without Cu2+. EGCG in F-12 medium was quantified using HPLC and the viability of cells was assessed by gel electrophoresis, flow cytometry, and electron microscope. The results of HPLC showed that EGCG degraded completely within 12 h in F-12 medium with or without Cu2+. Gel electrophoresis and flow cytometry did not detect apoptosis of LNCaP cells when they were incubated with EGCG. Electron microscopy examination revealed that EGCG-Cu2+ complex led to damage of cytoplasm membrane in LNCaP cells. It was speculated that not EGCG, but its oxide and complex with Cu2+, are the bioactive components responsible for its cytotoxicity to LNCaP prostate cancer cells.
Anticarcinogenic Agents ; administration & dosage ; Apoptosis ; drug effects ; Catechin ; administration & dosage ; analogs & derivatives ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cell Survival ; drug effects ; Copper ; administration & dosage ; metabolism ; Dose-Response Relationship, Drug ; Drug Combinations ; Humans ; Male ; Prostatic Neoplasms ; drug therapy ; pathology ; physiopathology