Objective To investigate the correlation between TNF-β NcoⅠpolymorphism and PDR.Methods PCR-RFLP analysis of frequencies of allele in each group.Results The frequency of allele TNF-β2 was significantly higher in patients with PDR than that without PDR（P<0.05）.Conclusion This polymorphism of TNF-β can be served as a susceptible genetic marker of PDR.

Objective To investigate the correlation between TNF-? NcoⅠpolymorphism and PDR.Methods PCR-RFLP analysis of frequencies of allele in each group.Results The frequency of allele TNF-?_2 was significantly higher in patients with PDR than that without PDR(P

Objective To document MRI findings of soft tissue damages involwing the temporomandibular joint (TMJ) after dislocated condylar fracture. Methods Seventy-seven subjects (103 TMJs), who suffered from the dislocated condylar fractures, underwent both sagittal and coronal MR imaging including proton density-weighted image ( PDWI ) and TzWI.Results On MR images, the TMJ structural changes after dislocated condylar fractures included : ( 1 ) displacement of the dislocated fragment in the anterior-inferior direction ( 102 TMJs, 99.0% ) and in the anterior direction ( 1 TMJ, 1.0% ) ; (2)disc displacement (100 TMJs, 97.1% ) in the anterior-inferior direction (99 TMJs) and in the anterior direction (1 TMJ) along with the dislocated condylar fragments on sagittal MR images; (3) joint effusion (103 TMJs, 100%);(4) abnormal signal intensity in the retrodiskal tissues of the TMJ ( 91 TMJs ,88.3% ) ; (5) abnormal changes of inferior posterior attachments of disc ( 89 TMJs, 86.4% ) ; ( 6 )abnormal changes of joint capsule ( 89 TMJs, 86.4% ) ; ( 7 ) abnormal changes of superior posterior attachments of disc ( 37 TMJs, 35.9% ) ; ( 8 ) disc deformation ( 8 TMJs, 7.8% ) ; ( 9 ) disc avulsion (8 TMJs, 7.8% ) and ( 10 ) glenoid fossa fractures (4 TMJs, 3.9% ). Conclusion On sagittal MR images, the TMJ soft tissue changes after dislocated condylar fracture were mainly presented as the disc displacement in the same direction (anterior-inferior direction) as the dislocation of the fractured fragments,usually in association with joint effusion.

Objective:To assesss the effectiveness of dapoxetine in the treatment of premature ejaculation.Methods:Both English and Chinese studies involving men with prematrue ejaculation who were treated with dapoxetine from the Cochrane Library,MEDLINE,EMBASE and CNKI,CBM,VIP between 1979 and 2009.were included in the randomized controlled trials(RCTs) and the data processed by RevMan.Results:Five RCTs involving 4433 patients were included in the Meta analysis,of which 3 were of grade A and 2 were of grade B according to the quality evaluation of methodology.Intravaginal ejaculatory latency time(IELT),patient-reported global impression of change(PGI),satisfaction with sexual intercourse(SWSI),perceived control over ejaculation(PCOE),personal distress related to ejaculation(PDRE) were used for assessment.Meta analysis based on included studies of patients having been treated with dapoxetine for 9-24 weeks showed that:(1) the difference of the patients' IELT between treatment group and control group was statistically significan [P

Objective To analyze the clinical characteristics of children infecting with Epstein -Barr virus. And in purpose to improve the knowledge of children infecting with Epstein -Barr virus.Methods We analyzed retrospectively to 97 cases of children which serodiagnosis of Epstein -Barr virus infectius in the aspects of the onset season,the age,clinical characteristics.And compared with complication group and no complication group in the aspects of the white cell count,neutrophils to white cell count ratio,lymphocyte to white cell count ratio and C -reactive protein.And contrasted the presence of statistical difference.Results The peak months were spring and summer(58 cases);Pre -school age was the peak age of EBV infectious(41 cases),and the rest respectively were toddlers age(33 cases),school age and youth(20 cases)and infant period(3 cases).The most frequently clinical manifestation respectively was pharyngitis,and other such as fever,lymphadenopathy,hepatosplenomegaly.There had no statistical difference in the two groups of complication group and no complication group in the aspects of the white cell count,neutrophils to white cell count ratio,lymphocyte to white cell count ratio and C -reactive protein(P >0.05).Conclusion The infant can suffer from infectious mononucleosis infect with EBV.Infectious mononucleosis have a variety of clinical manifestation,which always have some complications.The diognosis should combinate clini-cal manifestation with laberatory test.

Objective To review the clinical operation methods of abdominal incisional hernia. Methods Classification, operation method and fellow-up of 78 patients with abdominal incisional hernia were retrospectively analyzed. Results The average time of fellow-up was 26 months. Nineteen cases were repaired with simple suture with 3 cases (15.8%) recurrence, 57 cases were repaired with man-made material with 2 case (3.4%) recurrence. Conclusions Individual operation method should be chosen according to body condition, classification of the size of abdominal loss and abdominal hypertension. It is an effective method to repair the hernia of abdominal incision with man-made material.

Objective To explore the cause and treatment of chronic pain after tension-free repair of inguinal hernia.MethodsThe clinical data of 426 cases with inguinal hernia underwent the tension-free hernioplasty during February 2002 to September 2007 were retrospectively analyzed.ResultsTension-free hernioplasty was performed to all patients.According to operative methods,they were divided into two groups:polypropylene filling group(n=210)and expanded polytetrafluoroethylene(e-PTFE)mycromesh group(n=216).The chronic pain rate after operation,polypropylene filling group(9.0%,19/210)was significantly higher than e-PTFE mycromesh group(4.2%,9/216),P

Objective: To establish a method for determination of isofraxidin in Xiaoerkaiwei Oral Liquid (Radix Acanthopanacis Senticosi, Endothelium Corneum Gigeriae Galli, Semen Alpiniae Katsumadai, Semen Pharbitidis, etc.) by RP HPLC. Methods: The HPLC system consisted of Symmetry C 18 column(3.9m?150mm, 5?m), methanol water (0.1% formic acid) (30∶70) mixture as a mobile phase, detection wavelength at 342nm, 0.8mL?min -1 of flow rate and column temperature at 30℃.Results: The calibration curves of the isofraxidin were linear ( r =0.9993). The precision were perfect ( RSD =1.87). The samples were stable in 6 h. The average recovery of Xiaoerkaiwei Oral Liquid was 102.4% with RSD of 1.76%.Conclusion: This method is simple, quick and specific, suitable for the quality control.

Objective To explore the associated factors and clinical significance of acute renal injures in cerebral stroke.Methods The renal function,BUN,CR,UA patients with acute stroke in 7 days were estimated their neurologic impairment by the scardinanvian stroke scare(SSS) were assessed at the same time,then were compared with control group.Results The incidence of acute renal injure of intracerebral hemorrhage(ICH) group and cerebral infarction(CI) group were both higher than that of control group(P

Objective To explore the effect and mechanism of Gli2 on EMT and invasion in the hepatocellular car-cinoma cell line SMMC-7721.Methods shRNA of Gli2 and Negative control (NC) shRNA were constructed and transfected into SMMC-7721 cells.shRNA-Gli2 group,shRNA-NC group and blank group were set up .Transwell chambers assay , adhesion experiments were used to detect the ability of invasion ,homogeneous and heterogeneous cells intercellular adhesion of each group .Meanwhile, the qRT-PCR, Western blot were used to examine Gli2, E-cadherin ,N-cadherin and vimentin mRNA and protein expression .Results In different hepatocellular carcinoma cell lines and be along with the increasing ability of the invasion in hepatocellular carcinoma cell lines , Gli2 expres-sion was higher .Compared with the shRNA-NC group and blank control group ,the interfered group extensive cells invasion ability was inhibited ( P <0.05 ) , homogeneous cells intercellular adhesion increased and heterogeneous cells intercellular was opposite ( P <0.05 ) , meanwhile , the expression of E-cadherin was declined significantly ( P<0.05 ) ,the expression of N-cadherin , vimentin raised significantly ( P<0.05 ) .Conclusions Silencing Gli2 gene can reverse the EMT of the hepatocellular carcinoma cell line SMMC-7721 and inhibit cell invasion ,its mecha-
nism may be related to the up-regulation of E-cadherin and the dow N-regulation of N-cadherin, vimentin.