Objective To investigate the infection of human papilloma virus(HPV) and expression of serum squamous cell antigen (SCCAg) in the follow - up of 120 cervical squamous carcinoma patients who had received operation or operation combined with radiotherapy or only radiotherapy. Methods 120 cases of therapical cervical squamous carcinoma patients were detected HPV - DNA by HC - Ⅱ and serum SCCAg using immunohistochemical method. Results The positive rate of HPV - DNA and serum SCCAg was 49.17% and 17.50% , with a significant difference between them( P 0.05 ). Conclusion HPV - DNA test with HC - Ⅱ for follow - up of cervical squamous carcinoma patients was feasible. It was more sensitive than serum SCCAg. But it suggested that high risk type HPV -DNA test combined with serum SCCAg may be the independent prognostic factors.

Objective: To evaluate the expression of galectin-3(Gal-3) in cervical cancer tissues and its correlation with clinicopathological and survival variables. Methods: One hundred and seventy-four patients with International Federation of Gynaecology and Obstetrics (FIGO) stage I/II cervical carcinoma undergoing radical surgery were selected, and their cervical cancer tissues were collected. The expression of Gal-3 protein in cancer tissues was semi-quantitatively tested by tissue microarray and immunohistochemical analysis. Then the correlations of the expression of Gal-3 with the clinicopathological parameters, treatment outcomes and the survival of these patients were evaluated. Results: The positive expression rate of Gal-3 was 54.0% (94/ 174) in the cervical cancer tissues. In a univariate analysis, the positive expression of Gal-3 was closely correlated with non-squamous cell carcinoma (P=0.006), lymph vascular-space invasion (LVSI, P=0.048), deep-muscle invasion ( P= 0.001) and pelvic lymph-node metastasis (P<0.001). The multivariate analysis revealed that the expression of Gal-3 was one of the independent risk factors for predicting pelvic lymph-node metastasis. The risk factors including FIGO stage II (P<0.001), LVSI ( P=0.013), positive parametrium invasion (P=0.015), pelvic lymph-node metastasis (P<0.001) and the positive expression of Gal-3 (P=0.013) were significantly correlated with poor overall survival in these patients with cervical cancer. The tumor remission rate was lower in patients with up-regulated expression of Gal-3 (39.7%) than that in patients with down-regulated or invariant expression of Gal-3 (70.0%) after receiving preoperative radiotherapy. Conclusion: Positive expression of Gal-3 protein is a predictive factor for pelvic lymph node metastasis and worse overall survival in patients with cervical cancer. Up-regulated expression of Gal-3 protein may be associated with the resistance to radiotherapy.

Objective To investigate the quality assurance (QA) and quality control (QC)of intravenous injection in the radiology department. Methods The data from 1352 cases of intravenous injection in the radiology department were analyzed retrospectively. Results The bulge in region of the intravenous injection have been found in 28 cases, regional cyanochroia in 52 cases, allergic response in 25 cases, hypoglycemia response in 58 cases. Conclusion It is important to strengthen the QA and QC of intravenous injection in the radiology department.

Objective:To compare dexamethasone and granisetron for the prevention of PONV(Postoperative nausea and vomiting) after LC(Laparoscopic cholecystemy).Methods:A total of 80 patients were randomly divided into four groups(n= 20 each).A:dexamethasone 8 mg group;B:granisetron 3 mg,C:combined application group,D were the control group.All patients were given the similiar standardized anesthesia and operative treatment.PONV were assessed during the first 24 h after operations.Results:Group A,B and C got higher scores of PONV than the control group(P

The energy expenditure in the late stage of pregnancy was studied. The workers of three kinds including spinner, sewers and kindergartners were selected, and ten healthy pregnant women were matched to non-pregnant as subjects. Energy consumption was measured by indirect method and calculated from dietary survey. The results showed that the energy expenditure for spinner, sewer and kindergartner in pregnancy was 9.5474, 10.1253 and 8.7287 MJ/day, but for non-pregnant woman was 9.4453 10.1281 and 8.1567 MJ/day respectively. Calorie intake was 10.8910, 11.2424 and 9.9830 MJ/day for pregnant women, but 9.2215, 10.4474 and 8.6734 MJ/ day for the non-pregnant women respectively. The difference of energy consumptions between the pregnant and non-pregnant women was not significant, but the calorie intake for the pregnant women was signigicantly higher (0.8368 MJ/day) than the non-pregnant. According to the energy consumption, we recommend the calorie intakes in the late stage of pregnancy may increase 0.8368 MJ/day.

Objective To observe the clinical effects of a new method:non-optic nerve transecring evisceration combined with first stage hydroxyapatite orbital implantation.Methods One hundred and twenty two eyes (122 cases) were randomly divided into group A and group B,evisceration was first undertaken,the scleral wall was superior-temporally to inferior-nasally dissected and double scleral petals were placed before the implanted hydroxyapatite artificial eyeball.The difference between group A and group B was:in group A,the optic nerve was transected behind the eyeball,but in group B,a 2 mm outside the optic nerve scleral circle cutting was taken.Hemostasis time before implantation,tensile force during conjunctival suture,pain during surgery,palpebral fissure height after eye speculum wearing,operation time consuming,pain after surgery,conjunctival edema and congestion,and ocular prosthesis wearing time were compared between the two groups.Results In groups A and B,the intra-operative hemostasis time were (23.46 ± 6.96)mins and (5.49 ± 1.72)mins,the tensile force score during conjunctiva suture were 3.39±0.74 and 0.45±0.59,the score of pain in the first day were 2.8 ±0.68 and 1.47 ± 0.67,the ocular prosthesis wearing time after surgery were (6.27±2.73) and (3.07 ± 2.11)weeks,respectively.The differences of all the parameters above were with statistical significance between groups A and B (P＜0.01).During 2 years follow-up,no complications such as orbital implant exposure or infection happened.Conclusions Compared to traditional methods,the non-optic nerve transecting evisceration method has merits of less impairment,short time-consuming,less pain,and quicker postoperative recovery.

Objective To investigate the effects of exercise therapy and health education on the hospitalization and exercise endurance of patients with moderate or severe chronic obstructive pulmonary disease (COPD).Methods A total of 86 patients with moderate or severe but stable COPD were divided randomly into an observation group (n=46) and a control group (n=40).The patients in both groups accepted general respiratory medical therapy.The patients in the observation group also accepted additional pulmonary rehabilitation therapy which consisted of exercise and health education while the patients in the control group only accepted health education and rehabilitation training guidance by telephone instead of standardized pulmonary rehabilitation therapy.The average recurrence of hospitalization,length of stay,and total bed time of all of the patients was tracked for a year before and after enrolment.Their 6 minute walking distance (6MWD) and quality of life (QOL) as reflected by using a scale recommended by the Chinese Society of Respiratory Disease before and after entering the study were evaluated.Results There was no significant difference between the groups in the year before entering the study in terms of their average frequency of hospitalization,length of stay or total bed time.In the year after enrollment average frequency of recurrence requiring hospitalization,length of stay and total bed time in the observation group all decreased significantly compared with the previous year,and all were significantly lower than in the control group.There were no significant differences between the groups in average 6MWD or average QOL score at entry,but in the subsequent year both groups' results improved significantly.Conclusion Long term pulmonary rehabilitation can not only enhance exercise endurance,it also improves QOL and relieves the economic burden of COPD patients on society.

Acute Disease ; Child ; Cytokines ; blood ; Humans ; Purpura, Thrombocytopenic, Idiopathic ; blood ; immunology

This study aims to explore the inferior adhesion of the renal fascia (RF), and the inferior connectivity of the perirenal spaces (PS) with multidetector computed tomography (MDCT), and to investigate the diagnostic value of CT for showing this anatomy. From May to July 2012, eighty-two patients with acute pancreatitis presented in our hospital were enrolled into this study and underwent contrast-enhanced CT scans. All the image data were used to perform three dimensional reconstruction to show the inferior attachment of RF and the inferior connectivity of PS. The fusion of anterior renal fascia (ARF) and posterior renal fascia (PRF) next to the plane of iliac fossa were found on the left in 71.95% (59/82) cases, and on the right in 75.61% (62/82). In these cases, bilateral perirenal spaces, and anterior and posterior pararenal spaces were not found to be connected with each other. No fusion of ARF and PRF below the level of bilateral kidneys occurred on the left side in 28.05% (23/82) cases and on the right side in 24.39% (20/82). In these patients, the PS extended to the extraperitoneal space of the pelvic cavity and further to the inguinal region, and bilateral anterior and posterior pararenal spaces were not found to be connected with each other. Three-dimensional reconstruction on contrast-enhanced MDCT could be a valuable procedure for depicting inferior attachment of RF, and the inferior connectivity of PS.
Abdominal Cavity ; anatomy & histology ; Adult ; Contrast Media ; Fascia ; anatomy & histology ; Humans ; Image Processing, Computer-Assisted ; Kidney ; anatomy & histology ; Multidetector Computed Tomography ; Pancreatitis ; pathology ; Pelvis ; anatomy & histology ; Tomography, X-Ray Computed

Objective To investigate the effects of transforming growth factorβ1(TGFβ1)and β3 (TGFβ3)gene transfer on MMP-2,MMP-9 and TIMP-1 expression in hepatic stellate cells(HSC-T6).Methods TGFβ1 and TGFβ3 expression plagmids were constructed.The recombinant expression plasmid pcDNA3.1(+)-=TGFβ1 and pcDNA3.1(+).TGFβ3 were transfected or cotransfected into HSC-T6.At 24,48 and 72 h after transfection,the expression of MMP-2,MMP-9 and TIMP-1 mRNA were detected by real-time quantitative PCR,and the expression of MMP-2,MMP-9 and TIMP-1 protein were detected by Western blot.The recombinant expression plasmid pcDNA3.1(+).TGFβ1 was transfected into HSC-T6,and positive clones were selected by G418.The positive clones were transfected by the recombinant expression plasmid pcDNA3.1(+).TGFβ1,and the expression of MMP-2,MMP-9 and TIMP-1 were detected at 48 h after transfection.Results After transfection with peDNA3.1-TGFβ1,MMP-2 and TIMP-1 increaged remarkably in HSC-T6 cells(P＜0.05),but MMP-9 remained at the sanle level;After transfection with pcDNA3.1-TGFβ3,expression levels of MMP-2,MMP-9 and TIMP-1 mRNA were not changed,but TIMP-1 protein increased remarkably(P＜0.05);in cotransfection group,the expression of MMP-2 was higher than that in the blank and the control groups(P＜0.05),but MMP-9 level was not changed and TIMP-1was decreased compared with that in the TGF-β1 transfection group(P＜0.05).After TGFβ3was transfected into positive clones,the change of MMP-2 wag not significant(P＞0.05).but MMP-9 increaged and TIMP-1 decreased significantly at 48 h after transfection(P＜0.05).Conclusions TGFB3 may inhibit liver fibrosis by increase the activity of MMP-2 and MMP-9,and decrease the activity of TIMP-1.