1.The Clinical Observation the Effects of the Re-Therapeutic on the Later Stage of Sudden Deafness
Suoqiang ZHAI ; Ning YU ; Guiliang ZHENG ; Yuhua ZHU ; He QIN
Journal of Audiology and Speech Pathology 2010;18(1):17-18
Objective To observe the effects of the re-therapeutic on later stage of sudden deafness.Methods Patients who met the criteria for sudden deafness and showed poor response tO conventional therapy over two months were recruited and assigned randomly for re-therapy.The pure tone audiometry was conducted before and after re-treatment in 103 patients(112 ears).Sodium bicarbonate and dexamethasone were iniected by intravenous drip for two days and batroxobin 5 BU for 6 days.Statistics were made in the effecency of the re-treatment.Results The effective rate of re-treatment group was 46.43% and the difference was significant before and after retreatment(P<0.01).Conclusion Combined use of sodium bicarbonate dexamethasone and batroxobin is effective in re-treating later sudden deafness.
2.Clinical analysis of minimally invasive surgery and drug treatment for hypertensive cerebral hemorrhage
Yongcheng YU ; Huarong YANG ; Jianghuan ZHENG ; Lijie NING ; Guofeng WU
Chongqing Medicine 2013;(29):3505-3507
Objective To compare the clinical efficacy of minimally invasive removal of intracranial hematomas and medical con-servative treatment for hypertensive cerebral hemorrhage .Methods A total of 75 patients with hypertensive cerebral hemorrhage were collected and randomly divided into two groups according to the treatments :38 patients treated with conservative medicine and 37 patients treated with minimally invasive removal of intracranial hematomas based on standard drug therapy .Neurological impair-ment and recovery of self-care ability of patients in both groups before and after three weeks and six weeks of treatment were scored by National Institute of Health Stroke Scale (NIHSS) .Changes of diffusion tensor imaging before treatment and after two weeks of treatment were evaluated by fractional anisotropy value (FA) .Results There was no significant difference between neurological im-pairments of the patients in two groups before treatment .But the recovery degree of functional impairment and muscle nerve in min-imally traumatic treatment group was more significant than those in the conservative medical treatment group after treatments . There was significant difference between two groups (P< 0 .05) .Conclusion Minimally traumatic of hematomas is an effective method of treating hypertensive intracerebral hemorrhage .
3.Expression and functional role of p38MAPK in the kidney after unilateral ureteral obstruction in rats
Shenglang ZHU ; Xueqing YU ; Ning LOU ; Xunhua ZHENG ; Yongquan LI
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To investigate the expression and functional role of p38MAPK in the kidney after unilateral ureteral obstruction in rats. METHODS: Unilateral ureteral obstruction (UUO) models were induced by ligating the left ureter. Rats were sacrificed at 1 h, 3 h, 6 h, 12 h, 1, 3, 5, 7, 14, 21, and 28 days after UUO was initiated. p38MAPK activity was assayed by immunohistochemical staining and specific substrate phosphorylation with immunoprecipitation and Western blotting. TGF? mRNA and protein expression were analyzed with in situ hybridization and immunohistochemical stainning. RESULTS: A basic p38MAPK activity was detectable in the normal kidney(0.22?0.06). p38MAPK pathway was rapidly activated at 1 hour(0.45?0.14 vs control, P
4.Screening of effective shRNA targeting TNF-alpha and constructing of recombinant plasmid.
Xiao-Yu SONG ; Ning-Ning ZHENG ; Lu-Ning SUN ; Hai-Peng ZHANG
Journal of Experimental Hematology 2009;17(1):180-183
The objective of this study was to screen out the effective shRNA which can inhibit the gene expression of tumour necrosis factor-alpha (TNF-alpha), to construct the recombinant plasmid and to determine its sequence so as to provide the new approach for searching gene therapy of TNF-alpha related diseases. The primary macrophages were added into 15% DMEM, then cells were adjusted as 2 x 10(7) cells/L and were inoculated in 6-well plate with 3 ml/well, and were cultured at 37 degrees C in a fully humidified atmosphere with 5% CO(2). Cells were stimulated with lipopolysaccharide (LPS) and the concentration of TNF-alpha in the supernatant at different time points was determined by enzyme-linked immunosorbent assay (ELISA). The 5 synthesized DNA sequences which can be transcripted into shRNA were transfected into cells with lipofectamine 2000, then the cells were stimulated with LPS for 24 hours. The concentration of TNF-alpha in the supernatant and the expression of TNF-alpha mRNA were determined by ELISA and reverse transcription polymerase chain reaction (RT-PCR) respectively. The most effective shRNA was inserted into plasmid, and the recombinant plasmid was identified by sequence analysis. The results showed that the concentration of TNF-alpha in the supernatant reached peak after the stimulation with LPS for 24 hours. In the RNA interference group, the shRNA 1 was the most effective one, which could inhibit the expression of TNF-alpha by 59.46% and the expression of TNF-alpha mRNA by 61.2%. The recombinant plasmid was cloned and the sequence of interest was obtained. In conclusion, the most effective shRNA targeting TNF-alpha was successfully screened out and the recombinant plasmid was constructed. The recombinant plasmid may be helpful to search new gene therapy for TNF-alpha related disease.
Animals
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Cells, Cultured
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Gene Expression
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Genetic Therapy
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Genetic Vectors
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Macrophages
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cytology
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Male
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Mice
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Mice, Inbred C57BL
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Plasmids
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RNA Interference
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RNA, Messenger
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genetics
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RNA, Small Interfering
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genetics
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Recombination, Genetic
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Transfection
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Tumor Necrosis Factor-alpha
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genetics
5.Practice of SHDC in strengthening disciplines development of public hospitals
Peiyong ZHENG ; Rong ZHAO ; Guangjun YU ; Jiahong YANG ; Li YANG ; Ping HE ; Ning ZHENG ; Jiechun GAO
Chinese Journal of Hospital Administration 2015;(8):593-595
By means of joint research of new advanced technology project,joint development and application of appropriate health technology project,comprehensive prevention and control of chronic diseases between municipal hospitals and community clinics project and research supporting platform project,Shanghai Hospital Development Center has effectively improved the clinical science and technology innovation capability, promoted the development of medical disciplines and talents and improved the discipline influence of such hospitals.
6.Preparation, characterization and improved solubility of ticagrelor salts
Hong-mei YU ; Zheng-yu FANG ; Cheng XING ; Kun HU ; Ning-bo GONG ; Yang LÜ
Acta Pharmaceutica Sinica 2021;56(2):570-576
Four salts of ticagrelor, ticagrelor-3,5-dinitrobenzoic acid, ticagrelor-pyrazinamide, ticagrelor-
7.Effect of hyperbaric oxygen on acute graft-versus-host disease after allogeneic bone marrow transplantation.
Xiao-Yu SONG ; Lu-Ning SUN ; Ning-Ning ZHENG ; Hai-Peng ZHANG
Journal of Experimental Hematology 2008;16(3):623-626
The objective of this study was to investigate the function and mechanism of hyperbaric oxygen (HBO) in antagonizing acute graft-versus-host disease (aGVHD) and improving the rate of survival. The lethally irradiated C57BL/6 recipients were injected with bone marrow and lymphocyte of spleen from BALB/c donors and were treated with HBO, cyclosporine A (CsA) and methotrexate (MTX). T lymphocytes and subsets, adhesion molecules and cytokines were detected by flow cytometry, ELISA and RT-PCR respectively. The results showed that the survival rate in HBO group was much higher than that in allogenetic bone marrow transplantation (allo-BMT) group and CsA + MTX group; the numbers of CD3(+), CD4(+), CD8(+), CD4(+)CD11a(+), CD4(+)CD18(+), CD8(+)CD11a(+), CD8(+)CD18(+) lymphocytes in spleen were decreased markedly by HBO and CsA + MTX (p < 0.05); the levels of IL-2 and TNFalpha mRNA and their serum concentrations in HBO group were much lower than those in allo-BMT group but were higher than those in CsA + MTX group; the levels of IL-4 and IL-10 mRNA in HBO group were much higher than those in allo-BMT group and CsA + MTX group. It is concluded that HBO has more remarkable advantage in improving the rate of survival than CsA + MTX, its mechanism of anti-aGVHD is tightly correlated with the transform of T cell and its subsets and the expression of adhesion molecules and cytokines.
Acute Disease
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Animals
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Bone Marrow Transplantation
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adverse effects
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Cytokines
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biosynthesis
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Female
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Graft vs Host Disease
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etiology
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therapy
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Hyperbaric Oxygenation
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Lymphocyte Transfusion
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adverse effects
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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T-Lymphocytes
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immunology
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Whole-Body Irradiation
8.Effect of hyperbaric oxygen preconditioning on spleen lymphocytes and cell adhesion molecules after skin transplantation in mice.
Xiao-Yu SONG ; Lu-Ning SUN ; Ning-Ning ZHENG ; Hai-Peng ZHANG
Journal of Experimental Hematology 2010;18(5):1275-1277
The aim of this study was to explore the effect of hyperbaric oxygen (HBO) preconditioning on the rejection of skin allograft in mice and its molecular mechanism. BALB/c donor mice and C57BL/6 recipients received hyperbaric oxygen preconditioning once a day for 7 days. After skin transplantation, the recipients were treated with cyclosporine A (CsA) intraperitoneally. Immunofluorescent staining technique and flow cytometry were used to observe the influence HBO on percentage of spleen lymphocytes CD3+, CD4+, CD8+ and cell adhesion molecule LFA-1 (CD11a/CD18). The results showed that as compared with control, the numbers of CD3+, CD4+, CD8+, CD4+CD11a+, CD4+ CD18+, CD8+CD11a+, CD8+CD18+ lymphocytes of spleen decreased in HBO preconditioning groups and CsA group, and decreased markedly in HBO preconditioning combined with CsA group (p<0.05); the general state of recipient mice in HBO preconditioning combined with CsA group was better than that of recipient mice received HBO preconditioning or CsA only. It is concluded that the method of HBO preconditioning combined with traditional immunosuppressive agent CsA has remarkable advantage in inhibiting the rejection of skin graft. Its molecular protective mechanism is correlated with the expression of adhesive molecules on T cell subsets.
Animals
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Cell Adhesion
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Cell Adhesion Molecules
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pharmacology
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Cyclosporine
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pharmacology
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Female
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Graft Rejection
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prevention & control
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Graft Survival
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Hyperbaric Oxygenation
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Lymphocyte Count
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Lymphocytes
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cytology
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metabolism
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Skin Transplantation
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immunology
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Spleen
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cytology
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Transplantation Conditioning
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methods
9.Placement of a Long Intestinal Tube in Patients with Early Postoperative Small Bowel Obstruction under Fluoroscopic Guidance.
Zhi-wei WANG ; Xiao-guang LI ; Jie PAN ; Ning YANG ; Hai-feng SHI ; Zheng-yu JIN
Chinese Medical Sciences Journal 2015;30(3):156-161
OBJECTIVETo investigate the placement of a long tube into the small intestine under fluoroscopic guidance and to evaluate its decompression effect on early postoperative small bowel obstruction (EPSBO).
METHODSFifty-four patients with EPSBO requiring decompression between April 2010 and July 2014 were enrolled in the study. Insertion of a long tube was guided by fluoroscopy. We first used the guide wire to pass the pylorus and then used the 10 Fr feeding tube as an exchangeable tube to put the superstiff wire into the duodenum. Finally the long tube could be passed over the guide wire through the pylorus into the intestine. The total procedure time, the radiation exposure time, and the incidence of complications were evaluated.
RESULTSThe long tubes passed into the jejunum on initial insertion for all patients, so the success rate of this technique was 100%. The long tube was inserted into ileum in 18 patients. The mean total procedure time was 34.4 ± 8.6 minutes, and the mean radiation exposure time 18.9 ± 6.8 minutes. A total of 47 patients (87%) experienced full recovery following long-tube decompression and without the need for surgical intervention.
CONCLUSIONSUsing the wire-exchange technique, it is easy to place a long tube into the small bowel under fluoroscopic guidance. This decompression method is safe and effective for management of EPSBO.
Adult ; Aged ; Decompression, Surgical ; methods ; Female ; Fluoroscopy ; Humans ; Intestinal Obstruction ; surgery ; Male ; Middle Aged ; Postoperative Complications ; surgery ; Retrospective Studies
10.New dicyclopeptides from Dianthus chinensis.
Jing HAN ; Zhe WANG ; Yu-Qing ZHENG ; Guang-Zhi ZENG ; Wen-Jun HE ; Ning-Hua TAN
Acta Pharmaceutica Sinica 2014;49(5):656-660
One new dicyclopeptide cyclo-(L-N-methyl Glu-L-N-methyl Glu) (1), together with one new natural dicyclopeptide cyclo-(L-methyl Glu ester-L-methyl Glu ester) (2), and two known dicyclopeptides cyclo-(L-methyl Glu ester-L-Glu) (3), and cyclo-(L-Glu-L-Glu) (4), were isolated from the aerial parts of Dianthus chinensis L. Their structures were determined by spectroscopic analyses and chemical methods.
Dianthus
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Components, Aerial
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chemistry
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Plants, Medicinal
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chemistry