2.The effects of electromyographic biofeedback in the treatment of dysphagia after stroke
Yu MIN ; Haixia YAN ; Zhirui HUANG ; Yan GAO ; Zhen HUANG
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(8):583-586
Objective To investigate the efficacy of electromyographic biofeedback therapy (EMGBFT) in treating dysphagia after stroke.Methods Patients diagnosed with dysphagia after stroke were recruited and randomly divided into a control group (n =22),an electrostimulation group (ES,n =25) and an EMGBFT group (n =23).The control group received conventional treatment,while the patients in the other groups additionally received Vitalstim ES or EMGBFT 5 times per week for 3 weeks.Before and after the trial,deglutition function was evaluated through surface electromyography (sEMG) and using a standardized swallowing assessment (SSA).Results After 3 weeks of treatment,the mean sEMG amplitude,deglutition duration and SSA score and improved significantly in comparison to the baseline in all three groups.All were also significantly better in the two treatment groups than in the control group.Importantly,the mean sEMG amplitude,deglutition duration and SSA score were all significantly better in the EMGBFT group than in the ES group.Conclusion EMGBFT can promote better deglutition among patients with dysphagia after stroke more effectively than ES or conventional treatment.
3.Effects of Systems of Progressive Resistance Exercise on Quadriceps Femoris of Stroke Patients: a Surface Electromyography Analysis
Zhen HUANG ; Fen HUANG ; Taotao LI ; Peishun CHEN ; Yu MIN ; Zhiyong HUANG
Chinese Journal of Rehabilitation Theory and Practice 2009;15(11):1054-1055
Objective To observe the effects of systems of progressive resistance exercise on lower limb extensors (quadriceps femoris) of stroke patients. Methods 89 stroke patients with weak lower limbs were randomly divided into progressive resistance exercise group (n=46) and control group (n=43), both receiving corresponding treatment for 4 weeks. They were assessed with Manual Muscle Test (MMT), integrated electromyogram (iEMG) and root mean square (RMS) of surface electromyography on quadriceps femoris before and after the treatment. Results The incidence of improvement with MMT was 94.3% in progressive resistance exercise group, and 63.8% in control group (P<0.01). The iEMG and RMS of the exercise group was significantly higher than that of the control (P<0.01). Conclusion The systems of progressive resistance exercise could improve the muscle strength of lower limbs extensors of stroke patients more effectively.
4.The public health human resource of CDCs in Zhejiang Provincefrom 2016 to 2020
PANG Mengtao ; HUANG Liang ; LI Na ; HUANG Xuemin ; YU Didi ; YU Min
Journal of Preventive Medicine 2021;33(10):1009-1013
Objective:
To investigate the development of public health human resource of the Centers for Disease Control and Prevention (CDCs) in Zhejiang Province from 2016 to 2020, so as to put forward the strategies for the talent team construction of CDCs.
Methods:
The general information of personnel of CDCs in Zhejiang Province from 2016 to 2020 was collected through China Information System for Disease Control and Prevention. A questionnaire survey and a qualitative interview were conducted to collect the data of staffing, talent training, salary and employee turnover, and to analyze the development of the talent team of CDCs.
Results:
By 2020, there were 105 CDCs, including 1 provincial, 11 prefectural and 93 county-level CDCs, and 5 277 staff on-the-job. From 2016 to 2020, the number of staff per 10 000 permanent residents in the province, prefecture and county levels were 0.062-0.070, 0.170-0.188 and 0.585-0.604, respectively, which did not meet the standard for the personnel establishment in Zhejiang CDCs; the proportion of the staff with bachelor degree or above increased from 90.05% to 94.25%; the proportion of the health professionals decreased from 82.91% to 80.01%; the proportion of the staff with senior professional titles increased from 35.20% to 45.25%. The results of the qualitative interview indicated a lack of health professionals and high-end talents, a gap between the approved staffing and post requirements, as well as demands for a stable team and higher pay.
Conclusion
From 2016 to 2020, the structure of educational background, major and professional title among the on-the-job staff of CDCs in Zhejiang Province has been improving; however, the total allocation of human resource is insufficient, and the stability and treatment of staff need to be further improved.
5.Different active ingredients of medicinal plant based on function differentiation of homologous gene.
Yuan YUAN ; Jun YU ; Lu-qi HUANG ; Xu-min WANG
China Journal of Chinese Materia Medica 2015;40(6):1023-1026
In the research field of quality control in Chinese medicinal materials, variation in active ingredients of medicinal plant is always the key and hot issues. With the development of high-throughput sequencing technologies and reducing cost, a large numbers of genes from medicinal plant were cloning and provide a solid foundation for further research of gene structure and its biological function, and also provides conditions for explore active ingredient variation and its quality control from the perspective of molecular pharmacognosy. This paper introduces the concept of homologous gene, gene duplication and classification. We prospect the function of duplicated genes in the role of molecular mechanism research about variation in active ingredients, aiming at providing a new way for medicinal materials quality control.
Drugs, Chinese Herbal
;
analysis
;
Gene Duplication
;
Plant Proteins
;
genetics
;
Plants, Medicinal
;
chemistry
;
genetics
;
Quality Control
6.Prognostic value of heart rate turbulence in patients with chronic heart failure
Min GAO ; Hua YU ; Ying HUANG ; Weiping XU ; Qun LIANG
Chinese Journal of Geriatrics 2010;29(3):192-194
Objective To investigate the prognostic value of heart rate turbulence (HRT) in patients with chronic heart failure (CHF). Methods From October 2006 to May 2009, a total of 96 elderly CHF inpatients were selected, and the clinic data were recorded. Based on the echocardiogram and dynamic electrocardiogram, the differences of sinus HRT index of patients with different cardiac function classification were analyzed. During 9-28 months medical follow-up, the treatment endpoint was death from heart disease. Based on Logisitc regression, the prognostic values of HRT, age, hypertension, diabetes, myocardial infarction (MI), left ventricular ejection fraction, ACEI and β- adrenergic blocker for death of CHF patients were analyzed.Results There was no significant difference in HRT between grade Ⅱ and grade Ⅲ cardiac function (χ~2 = 1.60, P>0. 05), and between grade Ⅲ and grade Ⅳ cardiac function (χ~2 = 1.43, P>0. 05). But there was significant difference in HRT between grade Ⅱ and grade Ⅳ cardiac function patients χ~2 =9.84, P<0. 05), and HRT was weaken in grade Ⅳ group. The average follow-up time was (18. 0±9.6) months. Of all 96 patients, there were 34 dead of heart disease. There were correlations of death of CHF with HRT, low LVEF (≤ 45%), age (≥65 years), diabetes, MI and classification of heart function. Conclusions The sinus HRT in CHF patients has a favorable prognostic value.
7.Progressive resistance exercise for improving lower limb motor function in hemiplegic stroke patients
Zhen HUANG ; Yu MIN ; Peishun CHEN ; Taotao LI ; Tao ZHONG
Chinese Journal of Physical Medicine and Rehabilitation 2009;31(11):760-762
Objective To investigate the efficacy of progressive resistance exercise for improving the lower limb motor function of hemiplegic stroke patients. Methods Eighty-nine hemiplegic patients were randomly divided into a treatment group (n =46) and a control group ( n = 43 ) . Both groups received routine rehabilitation training, while the treatment group also undertook progressive resistance training. The control group received extra quadriceps femoris training. Before and after the treatment, motor function was evaluated with the Fugl-Meyer lower limb assessment, functional ambulation category (FAC) classification and the modified Barthel index (MBI). Results Before intervention, there were no significant differences between the two groups in any of the assessments. Four weeks later, all the assessment scores obtained with the Fugl-Meyer lower limb assessment, FCA classification and MBI in the treatment group were significantly better than those in the control group. Conclusion Progressive resistance training combined with routine rehabilitation treatment can improve motor function in the lower limbs, ambulation and ability in the activities of daily living of hemiplegic patients after stroke.
8.The effects of pretreatment with low-frequency transcranial magnetic stimulation on rats with pilocarpine-induced seizures
Min HUANG ; Juming YU ; Xiaoming WANG ; Li WANG
Chinese Journal of Physical Medicine and Rehabilitation 2009;31(4):228-231
Objective To study the effects of low-frequency transcranial magnetic stimulation (TMS) on rats with seizures induced by lithium-pilocarpine. Methods Rats were randomly divided into five groups and pre-treated with TMS at 75% of the stimulator's maximum output, but at 0 Hz (the sham group), 0.3 Hz, 0.5 Hz, 0.8 Hz and 1.0 Hz, respectively. After stimulation every day for 2 weeks, a model of acute epilepsy was induced u-sing iithium-pilocarpine. Subsequent behavior and the latencies of seizures were observed, and the severity of the sei- zures was quantitatively evaluated for 90 rain. Results The seizure latencies were significantly prolonged in all the other groups relative to the sham group (P<0.05). The prolongation in the 0.5 Hz and 0.8 Hz groups was signifi-cantly greater than in the 0.3 Hz and 1.0 Hz groups. The severity of seizures in the 0.3 Hz and 1.0 Hz groups was not significantly different from that in the sham group, but most of the rats in the 0.5 Hz group and the 0.8 Hz group experienced much lighter seizures, with seizure severity scores significantly lower than those in the other 3 groups. Conclusions Pretreatment with TMS at 0.3 to 1.0 Hz led to a longer latency of seizure onset. 0.5 Hz and 0.8 Hz gave the longest latencies and showed conspicuous antiepileptie effects.
9.Inhibition Function of Dominant-negative Mutant Gene Survivin-D53A to SPC-A1 Lung Adenocarcinoma Xenograft in Nude Mice Models.
Min YU ; Xingchen PENG ; You LU ; Meijuan HUANG
Journal of Biomedical Engineering 2015;32(3):624-628
Survivin-D53A (SVV-D53A) is a dominant-negative mutant survivin, which represents a potential promising target for cancer gene therapy. The present study was designed to determine whether SVV-D53A plasmid encapsuled by DOTAP: Chol liposome would have the anti-tumor activity against SPC-A1 lung adenocarcinoma, and to detect the possible mechanisms. In our experiment, SPC-A1 cells were transfected in vitro with SVV-D53A plasmid and examined for protein expression by Western blot, then flow cytometric analysis was used to detect apoptosis. SPC-A1 lung adenocarcinoma xenografts were established in vivo in the nude mice, which received the i. v. administrations of SVV-D53A plasmid/liposome complexes. After mice were sacrificed, the paraffin-embedded tumor tissue sections were used for proliferating cell nuclear antigen (PCNA) expression and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Compared with the control group, the mice treated with SVV-D53A plasmid had an obviously reduced tumor volume, with high level of apoptosis and decreased cell proliferation in tumor tissue. The research results proved that the administration of SVV-D53A plasmid resulted in significant inhibition of SPC-A1 cells both in vitro and in vivo. The functional mechanism is that the anti-tumor response causes and induces tumor cell apoptosis.
Adenocarcinoma
;
pathology
;
Animals
;
Apoptosis
;
Apoptosis Regulatory Proteins
;
metabolism
;
Cell Line, Tumor
;
Cell Proliferation
;
Heterografts
;
Humans
;
Inhibitor of Apoptosis Proteins
;
metabolism
;
Liposomes
;
Lung Neoplasms
;
pathology
;
Mice
;
Mice, Nude
;
Neoplasm Proteins
;
metabolism
;
Neoplasm Transplantation
;
Plasmids
;
Transfection
;
Tumor Burden
10. Application of heart rate and rating of perceived exertion scale in monitoring physical fatigue in divers during heliox saturation diving
Academic Journal of Second Military Medical University 2015;36(9):978-982
Objective To study the application of heart rate(HR) and rating of perceived exertion(RPE) scale in monitoring the physical fatigue in the divers during the 100 msw heliox saturation diving. Methods The 100 msw pressure environment was simulated by a 500 msw saturation diving system: pressure was 1.1 MPa; oxygen partial pressure was 35-52 kPa; carbon dioxide partial pressure was <0.5 kPa;the temperature during exposure to high pressure was (31.0±1.7)℃;and the relative humidity was (76.8±3.5)%. Respiratory gas was heliox. The 18 divers' HR and the scores of RPE scale were recorded at 6 time points, including (1)the day before the subjects entered the chamber; (2) the first day that the subjects were exposed to 100 msw pressure environment; (3) the peak of the subject physical exercise load test under 100 msw pressure environment; (4) the second day under 100 msw pressure environment; (5) the first day that the subjects were out of the chamber; and (6) the seventh day that the subjects were out of the chamber. All the data were analyzed with paired-samples t-test. The correlation was analyzed with Pearson correlation. Results From the day before the subjects entered the chamber to the first day that the subjects were exposed to 100 msw pressure environment, the resting HR of subjects was significantly decreased (P<0.05); From the first day that the subjects were out of the chamber to the seventh day that the subjects were out of the chamber, the resting HR was significantly increased (P<0.05). Significant differences were also found in the basic RPE score of the subjectes between before entering the chamber and after entering the chamber(P<0.05). During the 100 msw heliox saturation, with the variable physical work loads, the trend of of heart rate changes was still in accordance with the score of RPE scale, with that measured at the peak of the subject physical exercise load test under 100 msw pressure being significantly different from that measured at the first day the subjects were exposed to 100msw pressure (P<0.05).The RPE score was significantly related with the HR of divers (r=0.734, P<0.01). Conclusion The combined application of HR and RPE scale can monitor the physical fatigue of the divers during 100msw heliox saturation diving.