Objective To study the relationship of matrix metalloproteinase 7(MMP7)expression to the infiltration and metastasis of malignant melanoma.Methods Various concentrations(10,20,30 μmol/L)of heparanase antisense oligodeoxynucleotide (ASODN)was used to transfect human malignant melanoma cell line A375.Then,the transfected cells were subcutaneously inoculated into the right back of nude mice.Six weeks after the inoculation,transplanted tumors were isolated from the mice,and the levels of MMP7 protein expression were assessed by SP immunohistochemistry.The expression rate of MMP7 was also examined by SP immunohistochemistry in A375 cells and in tissue samples from patients with malignant melanoma(n=30),iunctional nevus(n=30)and normal human controls(n=15).Results The expression rate of MMP-7 was 83.33%(25/30),6.67%(2/30)and 0(0/15)respectively in malignant melanoma,iunctional nevus and normal skin tissue samples;there was a significant difierence in the positivity rate of MMP-7 between the malignant melanoma group and iunctional nevus group (x2=35.62,P=0.000)as well as normal control group(x2=28.12,P=0.000),but not between the junctional nevus group and normal control group(P＞0.05).The expression of MMP-7 in transplanted tumor was decreased to a varying degreee by three levels of ASODN,and the inhibitory effects of 30 mol/L ASODN were the strongest(P＜0.05).MMP-7 protein was also highly expressed in A375 cells.Conclusions The expression of MMP-7 in cutaneous malignant melanoma is higher than that in iunctional nevus and normal skin tissue.Hpa-ASPDN significantly inhibits the expression of MMP-7 in transplanted tumors in nude mice.MMP-7 is highly expressed in A375 cells.

Animals ; Apoptosis ; Female ; Male ; Nasal Mucosa ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Rats ; Rats, Sprague-Dawley ; Rhinitis, Allergic, Perennial ; metabolism ; pathology

ObjectiveTo investigate the expressions ofheparinase,matrix metalloproteinase 2 (MMP2) and tissue inhibitor of metalloproteinase 2(TIMP2) in malignant melanoma lesions and their significance.MethodsSkin specimens were obtained from the lesions of 30 patients with malignant melanoma,30 patients with melanocytic nevus and the normal skin of 15 healthy controls.Immunohistochemical SP method was used to detect the protein expression of heparinase,MMP2 and TIMP2.ResultsThe malignant melanoma tissue specimens significantly differed from the melanocytic nevus and control tissue specimens in the expression rate of heparinase (63.33％ vs.6.67％ and 0.00,x2 =21.172,27.805,both P ＜ 0.01 ),MMP2 (70.00％ vs.13.33％ and 0.00,x2 =19.817,19.866,both P＜ 0.01) and TIMP2(60.00％ vs.6.67％ and 0.00,x2 =19.200,15.000,both P ＜ 0.01 ).ConclusionThe expression of heparinase,MMP2 and TIMP2 is significantly higher in malignant melanoma lesions than in melanocytic nevus lesions and normal skin tissue.

Objective To analyze the citation of papers published in Chinese Journal of Geriatrics from the year 2008 to 2012,and evaluate the quality and academic level of the journal.Methods Based on the information from Wanfang database,bibliometric analysis was conducted to analyze number of cited papers,citation frequency,citation time distribution,authors,funds supports etc,and immediacy index and citation half-life were calculated.Results A total of 1567 papers were published in the journal from the year 2008 to 2012,and 377 articles were cited for 669 times with citation rate of 24.06％.The average number of cited times was 1.77,and the top value per article cited was 18 times.There were 12 authors whose articles were cited 6 and over times,and 11 affiliations whose articles were cited more than 10 times.Among 377 papers,105 papers (27.85％) were supported by funds,among which the majority was provincial fund (36 papers) and national fund (35 papers).Immediacy index from the year 2008 to 2012 were 0.042,0.032,0.019,0.067 and 0.006,respectively,and the citation half-life was 1.71 in the year 2012.Conclusions Articles published in Chinese Journal of Geriatrics have high-quality and can reflect the development and research progress in geriatric medicine.The journal is one of the most important information resources in geriatric academic field in China.

Objective To analyze the citation characteristics of literatures in Chinese Journal of Geriatrics published from 2008 to 2012.Methods According to the information from Wanfang database,articles in Chinese Journal of Geriatrics published from 2008 to 2012 were surveyed.The quantity,type of languages and the self-cited rate,etc were analyzed,and the Price's index,half life of literatures were calculated.Results Of the 1567 papers published in the journal from 2008 to 2012,1479 papers cited 16054 literatures,with a citation rate of 94.4％.The average number of citations in each paper was 10.25.The major language of citation was English (73.33％),followed by Chinese (26.67％),and only one citation was in Japanese.The self cited rates of author and periodical were 1.20％ and 5.23％,respectively.The Price's index was 45.71％ and the half-life of literatures was 4.27 years.Conclusions The authors of Chinese Journal of Geriatrics cite a wide range of literature,and the journal plays an important role in publicizing research achievements of geriatric medicine.However,the average number of citations in each paper,timeliness and novelty of cited literatures,diversity of citation languages need further improving.

Objective To analyze the structural,content and language characteristics of English abstract in domestic versus international geriatrics journals in order to promote international exchange in geriatric medicine research in our country.Methods In this paper,we analyzed the characteristics and contents of original articles published in 5 foreign journals (IF＞3.0) from Pubmed database (n=60) and 5 core (the source of statistics) domestic geriatric journals from Wanfang web during recent 3 years (n=61).Results Our survey showed that in the domestic geriatrics journals,the structured abstracts were used with inflexible four-heading and fixed leading words.In foreign geriatrics journals,however,non-structured abstracts were mostly applied in articles on basic research,and structured abstracts primarily appeared in clinical or survey research papers.Compared with foreign geriatrics journals,the shortages or some problems in English abstracts of the domestic geriatrics journals included a short writing,repetition of objective with title,lack of description of research background,improper description of results data,more passive voice,improper use of modal verbs.Conclusions The domestic geriatrics journals should learn the diverse forms of abstracts and content structure from foreign geriatrics journals,strengthen the elaboration of background and highlights,and use active voice in concordance with the international mainstream.

Objective To explore the distribution and research status of literatures on Alzheimer disease (AD) published recently,to compare the differences in the research papers on AD between China and foreign countries,and to provide bibliometrics data for Alzheimer disease research.Methods Using bibliometrics methods,the characteristics and contents of Alzheimer disease research documents were analyzed in Pubmed database and Wanfang web by analysis tools.Research status of Alzheimer disease including the type of literatures and journal distribution from 2008 to 2012 was analyzed.Currently hot topics or top high frequency words were emphatically analyzed.Results There were 646 matched literatures for Alzheimer disease retrieved in Chinese core periodicals,and 19 423 literatures in Pubmed database.The articles for clinical study of Alzheimer disease were more than experimental and epidemiologic study among the 646 literatures.The disciplines distribution of AD in Chinese core journals was similar to international journals,mainly including nerves,brain,and geriatrics journals.The domestic research papers on Alzheimer disease were less than 5％ in Pubmed database,and the articles from Chinese journals in Chinese or English edition accounted for only a small proportion of Pubmed database.The recent research direction of Alzheimer disease in China was similar to foreign countries such as amyloid proteins,diagnosis and gene,but foreign research paid more attention to the metabolism,image and the aging mechanism in AD,while in China there were quite a lot of reports in the literatures on cholinergic drugs,tau protein,hippocampus and Chinese herbal treatments.Conclusions The AD-related disciplines distribution in China and foreign country is relatively fixed in recent 5 years.The AD researches carried out in China are close to those in foreign country,but researches on some areas still lag behind.The experimental and epidemiologic study on AD should be strengthen in China.

Objective To analyze the literature distribution and research status of osteoporosis in the elderly published in Chinese Medical Association journals.Methods The literatures of elderly osteoporosis published in Chinese Medical Association journals from 2008 to 2013 were collected.And the publication year,distribution,content,institution and region of the literatures were analyzed.Results From 2008 to 2013,there were 76 articles about osteoporosis in the elderly published in 22 kinds of journals of the Chinese Medical Association.Among these journals,the Chinese Journal of Geriatrics-published elderly osteoporosis articles occupied the most number [28 (36.8％)].There were 21 articles published mostly in 2013 (27.6％),secondly in 2009 (17 articles,22.4％) and 2012 (12 articles,15.8％).The province or city that published more than 10 articles was Beijing (18 articles,23.7％),Zhejiang (13 articles,17.1％),Shanghai (10 articles,13.2％) and Guangdong (10 articles,13.2％).The institution that published most articles was Beijing Jishuitan Hospital (4articles,5.3％).The main contents of these literatures included the influencing factors,diagnosis,treatment strategy,bone metabolism and surgery treatment for elderly osteoporosis.Conclusions Chinese Medical Association journals are the important source to acquire osteoporosis literature for the medical workers.The distribution of journal is relatively concentrated,but the distribution of authors,institution is dispersed.Chinese Journal of Geriatrics publishes the most number of articles.The research of osteoporosis has attracted more attention.

Objective To study the basic expression and cellular localization of SOCS-3 in normal rat retina. Methods Neuro-immunocytochemistry techniques were used. Results SOCS-3 positive cells were widely distributed in the ganglion cell layer (GCL) and inner nuclear layer (INL) in the retina. In the GCL, SOCS-3 immunoreactivity was mainly in the neuclei of the ganglion cells.Some of the SOCS-3 positive cells in INL were M?ller cells.Conclusion Basal expression of SOCS-3 is widely present in the neurons and glia in normal adult rat retina and mainly in the form of nuclear protein.