1.Expression of Interleukin-1β in Synovia and Synovium of Rats after High Intensity Exercise
Jingxin ZHAO ; Yu WANG ; Man HE ; Yu JIN ; Lunhao BAI
Chinese Journal of Rehabilitation Theory and Practice 2013;19(5):448-451
Objective To study the expression of interleukin-1β (IL-1β) in synovia and synovium of rats after high intensity exercise.Methods 24 healthy male Sprague-Dawley rats were randomly divided into control group and high intensity group, and the latter were forced to run 60 min at the speed of 28 m/min for 6 weeks. The histological changes of cartilage from the lesion areas of medial femoral condyle and synovium of knee joint were examined with HE stain and the expression of IL-1β in synovium with immunohistochemistry, in synovial fluid with the enzyme-linked immunosorbent assay (ELISA). Results The articular cartilage of knee were injured and synovitis were found in high intensity group. The expression of IL-1β both in synovial fluid and in synovium of the knee were more in high intensity group than in control group (P<0.05). Conclusion High intensity exercise could cause aticular cartilage injury, synovitis and over expression of IL-1β in synovia and synovium, which may associated with post-traumatic osteoarthritis.
2.Effects of intra-articular hyaluronan injection on the expression of cartilage oligomeric matrix protein in synovium of strenuous running rats
Lunhao BAI ; Yu WANG ; Man HE ; Yangyang LIN
Journal of Chinese Physician 2011;13(3):331-333,336
Objective To investigate the effects of intra-articular hyaluronan(HA)injection on the expression of cartilage oligomeric matrix protein(COMP)in synovium of strenuous running rats,and investigate the possibility of predicting the effectiveness of HA based on COMP in synovium.Methods 36 healthy male Wistar rats were randomly divided into control group,strenuous running group and strenuous running group and HA injection group.Strenuous running group and HA injection group were intra- articularly injected with HA once a week for 5 consecutive weeks.The histological changes of synovium of knee joint was examined by H.E.staining and immunohistochemical expression of COMP in three groups after 6 weeks' strenuous running.Results Synovial inflammation was less severe in strenuous running and HA injection group than strenuous running group(t =7.15,P <0.01).The immunohistochemical expression of COMP in rats'synovium of knee joint in strenuous running and HA injection group was significantly lower than that in rats'synovium in strenuous running group(t = 6.30,P < 0.01).Conclusions Intra- articular HA injection suppressed synovitis,and the expression of COMP in synovium could be used to predict the effectiveness of HA.
3.The Expression and Significance of Myeloperoxidase in Acute Lungs Injury of Severe Acute Pancreatitis Associated Ascetic Fluid
Man HE ; Yongyong LIU ; Nong CAO ; Chen CHAI ; Binsheng WANG ; Yongjiang YU
Journal of Medical Research 2006;0(05):-
Objective To investigate the expression and significance of myeloperoxidase(MPO) in acute lungs injury of severe acute pancreatitis associated ascetic fluid. Methods Forty-five adult wistar rats were randomly assigned into the group of negative control (group C,n=15),the group of severe acute pancreatitis (group S,n=15) and the group of peritoneal injection (group E,n=15). The group C was cut peritoneum and flipped pancreases softly. In group S,3.5% sodium taurocholate was injected retrograde in pancreatic and bile duct to establish the model of severe acute pancreatitis,and the pancreatic homogenate and ascites of the group S was injected into abdominal cavity of group E rats. After animal model established,rats were killed at 3h,6h and 12h point. The blood of inferior vena cava was sucked for determination of amylase.The inferior lobe of left lung was cut for myeloperoxidase detection.And pathology was regularly done about pancreas and lungs. Results Interstitial edema,hemorrhage and infiltration of neutrophilic granulocyte and macrophage were observed in group S and E. At different time point,the amylase levels of blood and myeloperoxidase of lungs in group S and E were significantly higher than those of group C,and the increasing degree of group E was smaller than group S. Conclusion Acute lung injury can be induced by the severe acute pancreatitis associated ascetic fluid. The expression of myeloperoxidase of lungs was increased to induce acute lungs injury.The reason may be concerned with activation of granulocyte by severe acute pancreatitis associated ascetic fluid.
4.Construction and anti-tumor efficiency evaluation of redox-responsive micelles for the co-delivery of IR-780 and 18β -glycyrrhetinic acid
Wen-jing BAI ; Chun-yu XIA ; Man LI ; Zheng-ze LU ; Qin HE
Acta Pharmaceutica Sinica 2022;57(1):211-221
Photothermal therapy (PTT) is a highly effective anti-tumor method. However, when laser radiation was used to ablate tumors, it usually triggers a series of inflammatory reactions, promoting the further development of tumors and affecting the effect of anti-tumor therapy. Therefore, it is an effective method to improve the anti-tumor effect by suppressing the inflammatory response through the precise targeted delivery of anti-inflammatory drug while realizing the photothermal treatment of tumors. To this end, the redox-responsive linker 3,3'-dithiodipropionic acid was used to bond the classic hydrophobic anti-inflammatory drug 18
5.Application of traditional Chinese medicine in the prevention and treatment of new sudden infectious disease.
Ning ZHANG ; Yu-ming GUO ; Li-fu WANG ; Man GONG ; Ning DU ; Xiao-he XIAO
Acta Pharmaceutica Sinica 2015;50(12):1534-1537
There has been thousands of years' history that traditional Chinese medicines were used in the prevention and treatment of infectious disease. In recent years, traditional Chinese medicine plays a unique role in the control of variety of new infectious diseases. This article provides a summary on our knowledge of the traditional Chinese medicine theory in the explanation of infectious disease, application of Chinese medicines and the pharmacological mechanism in the successful management on the Ebola virus disease.
Drugs, Chinese Herbal
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therapeutic use
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Hemorrhagic Fever, Ebola
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therapy
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Humans
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Medicine, Chinese Traditional
6.Study on Rapid Methods for Quantitative Analysis Rhamnolipid and Its Influence Factors
Guo-Man LU ; Hong-Yu LIU ; Guang-Ming ZENG ; Guo-He HUANG ; Hui ZHANG ;
Microbiology 1992;0(04):-
In order to find an easy and rapid quantitative analytical method to detect rhamnolipid produced by Pseudomonas aeruginosa, three methods, H_ 2 SO_ 4 -anthrone analysis method, L-cysteine-H_ 2 SO_ 4 method and phenol-H_ 2 SO_ 4 method, were compared in the present paper, and the influence factors were also considered.The results showed that H_ 2 SO_ 4 -Anthrone analysis method was better than the others and its optimal reaction condition was obtained.The influence to the quantitative analysis of rhamnolipid from the residual glucose and the top clean liquid layer in the ferment solution could be ignored.But the influence from the bacterial body and the middle layer of the ferment solution reached a certain degree.Thus, the bacterial body should be removed before measuring.However, the influence from the middle layer of the ferment solution could be avoided by making a standard curve which was made by using a rhamnose mixed with the middle layer ferment solution.
7.Dynamic protein-protein interaction subnetworks of lung cancer in cases with smoking history.
Wei YU ; Li-Ran HE ; Yan-Chao ZHAO ; Man-Him CHAN ; Meng ZHANG ; Miao HE
Chinese Journal of Cancer 2013;32(2):84-90
Smoking is the primary cause of lung cancer and is linked to 85% of lung cancer cases. However, how lung cancer develops in patients with smoking history remains unclear. Systems approaches that combine human protein-protein interaction (PPI) networks and gene expression data are superior to traditional methods. We performed these systems to determine the role that smoking plays in lung cancer development and used the support vector machine (SVM) model to predict PPIs. By defining expression variance (EV), we found 520 dynamic proteins (EV>0.4) using data from the Human Protein Reference Database and Gene Expression Omnibus Database, and built 7 dynamic PPI subnetworks of lung cancer in patients with smoking history. We also determined the primary functions of each subnetwork: signal transduction, apoptosis, and cell migration and adhesion for subnetwork A; cell-sustained angiogenesis for subnetwork B; apoptosis for subnetwork C; and, finally, signal transduction and cell replication and proliferation for subnetworks D-G. The probability distribution of the degree of dynamic protein and static protein differed, clearly showing that the dynamic proteins were not the core proteins which widely connected with their neighbor proteins. There were high correlations among the dynamic proteins, suggesting that the dynamic proteins tend to form specific dynamic modules. We also found that the dynamic proteins were only correlated with the expression of selected proteins but not all neighbor proteins when cancer occurred.
Databases, Genetic
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Databases, Protein
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Humans
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Lung Neoplasms
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etiology
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metabolism
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pathology
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Protein Interaction Mapping
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Protein Interaction Maps
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Smoking
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adverse effects
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Support Vector Machine
8.Correlation between anxiety among public hospital medical personnel and social support, perceived social support and coping styles
Yun-juan HE ; Man-man LU ; Gui-mei CHEN ; Meng-jie WANG ; Ming-zhuang CHEN ; Yu-ting SHE ; An-qi YAO
Chinese Journal of Disease Control & Prevention 2019;23(3):328-331
Objective This research aimed to explore the mental status of public hospital medical personnel and social support for them, social support they perceive, and their coping styles. This study was designed to investigate on the relationship between their anxiety and social support, perceived social support and their coping styles. Methods A questionnaire was adopted to collect data, including participants’ background, measurement of anxiety, as well as social support, perceived social support and their coping styles. Results(1)The anxiety level of medical personnel was significantly higher than that of domestic norm 37.23±12.59(t=14.370, P<0.001);(2)Anxiety levels appeared to be significantly different in occupation, educational background and working years;(3)Results shown in the three scales were different among participants with different levels of anxiety in perspectives of every dimension in the scales; (4) With the help of Spearman correlation analysis, it showed that anxiety levels of medical personnel was negatively correlated with objective support (r=-0.098, P=0.051), subjective support (r=-0.189, P<0.001) ,utilization of social support (r=-0.164, P=0.001), friend support (r=-0.356, P<0.001),family support (r=-0.330, P<0.001),other support (r=-0.238, P<0.001) and positive response (r=-0.282, P<0.001), and positively correlated with negative response (r=0.385, P<0.001). Conclusions Mental health of medical personnel should be noticed and enhanced, and social support for public hospital medical personnel should be strengthened. Thus, the performance of the medical team can be developed in order to support further public medical service construction.
9.Antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin against herpes simplex virus 2 in vitro.
Li-li HE ; Jiang-man DUAN ; Jia-yin QIU ; Fei YU ; Shu-wen LIU ; Lin LI
Journal of Southern Medical University 2011;31(7):1175-1178
OBJECTIVETo investigate the antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin (HP-OVA) against herpes simplex virus 2 (HSV-2) in vitro.
METHODSBy chemical modification, ovalbumin (OVA) was treated with 3-hydroxyphthalic anhydride (HP) to prepare HP-OVA. The anti-HSV-2 activity against HSV-2 333 virus in vitro and the cytotoxicity of HP-OVA in African green monkey kidney cells (Vero cells) were detected by MTT colorimetric assay. The inhibitory effects of HP-OVA on 17 strains of vaginal lactobacilli were observed by microscopy.
RESULTSAnhydride-modified ovalbumin significantly inhibited the infection by HSV-2 with an IC(50) of 23.56±8.33 µg/ml. HP-OVA showed only low cytotoxicity to the host cells with a CC(50) over 1 mg/ml. HP-OVA did not produce significant inhibitory effect on the 17 strains of vaginal lactobacilli (MIC>1 mg/ml).
CONCLUSIONAnhydride-modified protein HP-OVA exhibits potent anti-HSV-2 activity in vitro and can be a good microbicide candidate for prevention of sexually transmitted diseases.
Animals ; Antiviral Agents ; pharmacology ; Cercopithecus aethiops ; Herpesvirus 2, Human ; drug effects ; Ovalbumin ; chemistry ; pharmacology ; Phthalic Anhydrides ; chemistry ; pharmacology ; Vero Cells
10.Advances in the study of derivatization of ginsenosides and their anti-tumor structure-activity relationship.
Man CAO ; He-Shui YU ; Xin-Bo SONG ; Bai-Ping MA
Acta Pharmaceutica Sinica 2012;47(7):836-843
Ginsenosides, belonging to a group of saponins with triterpenoid dammarane skeleton, show a variety of pharmacological effects. Among them, some ginsenoside derivatives, which can be produced by acidic and alkaline hydrolysis, biotransformation and steamed process from the major ginsenosides in ginseng plant, perform stronger activities than the major primeval ginsenosides on inhibiting growth or metastasis of tumor, inducing apoptosis and differentiation of tumor and reversing multidrug resistance of tumor. Therefore ginsenoside derivatives are promising as antitumor active compounds and drugs. In this review, the derivatization methods, ginsenoside derivatives and their anti-tumor structure-activity relationship have been summarized for providing useful information for the research and development of novel antitumor drugs.
Antineoplastic Agents, Phytogenic
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chemistry
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isolation & purification
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metabolism
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pharmacology
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Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Ginsenosides
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chemistry
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isolation & purification
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metabolism
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pharmacology
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Humans
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Hydrolysis
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Neoplasms
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pathology
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Panax
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chemistry
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classification
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Plants, Medicinal
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chemistry
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Structure-Activity Relationship