Objective To evaluate the toxicological safety of Shanze weight-reducing food in order to provide some toxicological data for its application. Methods According to the national standard of safety evaluation, the following experiments of acute toxicity test through mouth in rats, the genetic toxicity test, thirty days feeding test and teratogenicity test in mice were performed. Results LD 50 was more than 20.0 g?kg -1, equivalent to 467 times of the recommended dosage, in acute toxicity test through mouth in both male and female rats, which fell into the scale of non-toxicity. Under the dosage range of 1.05 g?kg -1 to 4.20 g?kg -1, equivalent to 100 times of the recommended, the negative results were observed in the genetic toxicity test of micronucleus test of bone marrow cells, sperm shape abnormality test in mice and the Ames test. The negative impacts on the growth, development, hematology, biochemistry, histology and the ratio of organ to whole body were not found on the experimental rats of thirty days feeding. Under the same dosage range, no teratogenic effects were found in the experiments of teratogenicity test. Conclusions Shanze can be used as a kind of health weight-reducing food with toxicological safety under the experimental dosage range of 1.05 g?kg -1 to 4.20 g?kg -1.

0. 05). The treatment group in the improvement of most clinical symptoms were superior to the control group (P

Objective To study the incidences of falls in patients with Parkinson's disease and analyze the related factors.Methods The clinic data of 100 Parkinson's patients were retrospectively analyzed to investigate the fall incidence and the related factors.Results The fall incidence was 46.0%during hospitalization in the patients with Parkinson's disease.The single factor analysis showed that the falls incidence were higher in male patients,dizziness from drug factors,a history of falls and non-presence of their families during hospitalization than those in the non-fall group(P<0.05).Logistic regression analysis showed that male patients,dizziness from drug factors,a history of falls and non-presence of their families during hospitalization were risk factors for Parkinson's patients during hospitalization.(all P<0.05). Conclusions The high risk factors for the falls in Parkinson's patients include male patients,dizziness from drug factors,a history of falls and non-presence of their families during hospitalization.Therefore,the assessment of the risk factors for falls should be done so as to carry out fall factors control and fall risks classification management for the purpose of reducing falls.

Transcatheter arterial chemoembolization (TACE) is the first choice of patients with hepatocellular carcinoma (HCC) missed opportunity of exairesis.Angiogenesis is one of the key factors that affect prospective efficacy.Counter-measures against angiogenesis mainly contain complete embolization of tumor blood supply and integration with angiogenesis inhibitors.The angiogenesis after TACE and the corresponding countermeasures were reviewed in this article.

Aiming at the weakness of the medical graduate students' ideological and political education,we developed the new mode via investigation and surveying,allowing for the medical characteristics. We suggested that we should bring into full play the tutors and their groups function and excavate the connotation of groups’ morality education on the basis of the scientific research groups to develop the medical students' ideological and political education.

Background Cardiomyocytes apoptosis was related to oxidative stress which has been shown to play an important role in ventricular remodeling after myocardial infarction(MI).Probucol is a hypolipidemic and antioxidant agents.Several reports demonstrated its cardioprotective effect after MI.However,the exact effect of probucol on the modification of the apoptosis related gene Bcl-2 and Bax is not clear.Objective To investigate the effects of probucol on mRNA and protein expression of Bcl-2,Bax and oxidative stress in myocardial infarcted rats.Methods Forty-one SD rats that survived 24 h after ligating left anterior descending coronary artery were randomly to receive placebo-saline(5 mL/d,n=20)or probucol(probucol 60 mg/kg?d,n=21).Twelve rats underwent sham operation were served as control(n=12).Six weeks after treatment,hemodynamic pararmeters and left ventricular function were measured with catheterization.Cardiomyocytes apoptosis were determined by TUNEL method.Myocardium mRNA of Bcl-2 and Bax expression levels in the non-infarcted myocardium were as- sessed by RT-PCR.The myocardium protein expression of Bcl-2 and Bax in the non-infarcted myocardium were de- termined by Western blot.Colorimetry was used to determine oxidative metabolism index in myocardium.Results 1)Compared with the sham rats,all MI rats showed marked decrease in Bcl-2 mRNA and protein expression in myo- cardium with increase of Bax mRNA and protein expression and apoptosis index(P

Objective To evaluate the effect of penehyclidine hydrochloride (PHC) on the level of angiopoietin-1 (Ang-1) and tyrosine kinase receptor-2 (Tie-2) during endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 180-220 g,were randomly divided into 4 groups using a random number table (n =10 each):control group (group C),ALI group,low-dose PHC group (group L-PHC) and high-dose PHC group (group H-PHC).ALI was induced with iv injection of lipopolysaccharide 5.0 mg/kg via the tail vein.In L-PHC and H-PHC groups,PHC 0.6 and 2 mg/kg were injected,respectively,via the tail vein at 1 and 24 h after lipopolysaccharide injection.The rats were sacrificed at 48 h after the initial injection of PHC to measure the lung water content,protein concentration in bronchoalveolar lavage fluid (BALF),and the expression of Ang-1,Tie-2 and phosphorylated Tie-2 in lung tissues.The morphological changes of lung tissues were observed under light microscope and the ultrastructural changes of alveolar epithelial barrier under transmission electron microscope.Results Compared with group C,the lung water content and protein concentrations in BALF were significantly increased,and the expression of Ang-1 and phosphorylated Tie-2 was down-regulated in the other three groups (P ＜ 0.05).Compared with group ALI,the lung water content and protein concentrations in BALF were significantly decreased,and the expression of Ang-1 and phosphorylated Tie-2 was up-regulated in H-PHC group (P ＜ 0.05),and no significant changes were found in the parameters mentioned above in group L-PHC (P ＞0.05).The damage to lung tissues was significantly reduced in group H-PHC as compared with group ALI.Conclusion PHC can improve the permeability of pulmonary microvascular and reduce injury to alveolar epithelial barrier,thus ameliorating endotoxin-induced ALI in rats,and the effect is dose-related and up-regulation of Ang-1 expression and inhancement of Tie-2 activity are involved in the mechanism.

AIM: To compare the renoprotective effects of angiotensinⅡtypeⅠreceptor antagonist (AT 1RA) with that of angiotensin converting enzyme inhibitor (ACEI) and to investigate their influences on intrarenal renin-angiotensin system. METHODS: Experimental nephrotic syndrome model was induced in SD rats with repeated peritoneal injections of puromycin. Twenty-eight rats were randomly divided into four groups: normal control, nephrotic control, ACEI-treated and AT 1RA-treated group. Serum, urine and renal tissue were collected for study 12 weeks later. RESULTS: The urine protein was less and renal function was better in both treated groups. The glomerular and interstitial injury indexes of both ACEI and AT 1RA treated rats were lower than that of nephrotic control rats and had no significant difference between the two treated groups. The renal local ACE activity and angiotensinⅡ of nephrotic control group were significantly higher than that of normal control group and the two treated group(P

This paper describes the inhibitory effects of antineoplaston A10 with using different dosages against the mouse S180, rats W256 and Bca - P6 and MGC cell (human) in nude mouse. When the administered dosage of oral formulation antineoplaston A10 was 4000mg ? kg-1 ? 24h-1 against the S180 for 10d . inhibitory rate was 32. 5%( P

Objective: The regeneration and repair of peripheral nerves are focuses of neurological studies.The(bFGF) may play an important role in the development of the neural system.Dexamethasone is usually employed in the management of neural injuries.The aim of this study is to establish a rat model,and investigate the effect of Dexamethasone on the expression of bFGF in the neurons of the spinal cord following sciatic nerve injury.Methods: Fifty-six male Wistar rats were randomly divided into 4 groups: a nerve injury group,with sciatic nerve injury induced by clamping the nerve in the right;a dexamethasone group,immediately injected with dexamethasone [0.5 mg/(kg/d)] in the injury side;a saline group,given saline only;and a control group,rats without sciatic nerve injury.Changes of bFGF in the motor neurons of the L4-L6 spinal cords were observed by immunohistochemistry.Results: Dexamethasone obviously upregulated the expression of bFGF in the motor neurons of the spinal cord.Conclusion: Dexamethasone can improve the expression of bFGF in the motor neurons of the spinal cord after sciatic nerve injury,and contribute to the recovery of the function of the injured neurons.