Objective: To study the chemical constituents from Mentha haplocalyx. Methods: Compounds were isolated and purified by various chromatographic techniques, and their structures were identified by spectral analysis. Results: Eleven compounds were isolated and identified, including menthalignin (1), 5-hydroxy-6, 7, 8, 4'-tetramethoxyflavone (2), betulinic acid (3), hesperetin 7-O-β-D-glucopyranoside (4), gentisic acid 5-O-β-D-(6'-salicylyl)-glucopyranoside (5), linarin (6), acatin (7), 5-hydroxy-6, 7, 8, 3', 4'-pentamethoxyflavone (8), 5, 6, 4'-trihydroxy-7, 8-dimethoxyflavone (9), tilianin (10), and agastachoside (11). Conclusion: Compound 1 is a new lignan named menthalignan; compound 2 is a new natural product; compound 4 is isolated from the plants in Labiatae for the first time, and compounds 3 and 5 are firstly reported in the plants of Mentha L.

OBJECTIVETo explore the expression of hepatitis B-virus X gene (HBX) in adenoid cystic carcinoma (ACC) and determine its clinical significance.

METHODSBetween June 2008 and October 2012, in-hospital patients with salivary gland tumors who were treated at the Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, were enrolled to this study. HBeAb-positive patients were defined as those exposed to hepatitis B virus (HBV) or harboring persistent HBV infection regardless of being HBeAg positive or negative. According to the pathological results, all patients were divided into ACC group and control group. Immunohistochemical staining and polymerase chain reaction (PCR) were used to detect HBX expression in ACC group and control group.

RESULTSHBX expression was mostly detected in the cytoplasm of ACC cells. Minimal HBX expression was detected in the nucleus. HBX expression was significantly higher in ACC than in Warthin's tumor. A significant difference was observed between the two groups.

CONCLUSIONHBX is expressed in ACC and may be associated with the development of ACC. HBX might serve important functions in the carcinogenesis and development of ACC.

Objective:To study the biologic function of rhPLD2 mutation form.Methods:To adapt the guinea pig chronic asthma model was induced by OVA, the functions of rhPLD2 on PAF was observed through the assay of platelet congregating.Results:rhPLD2 remarkably reduced the lever of PAF in the serum of guinea pig chronic asthma model; compares with the NS group, the P

Objective To present the status of smoking control in the Health and Family Planning System in Hubei Province, and to provide scientific basis for smoking control through secret investigations. Methods During the period from July to August in 2015, 168 sanitary Health and Family Planning institutions were chosen by stratified selection to make questionnaires based on Evaluation Standards for Smoke-free Health and Family Planning Institutions issued by the National Health and Family Planning Commission of the People's Republic of China. Secret investigations were conducted in 165 sampled institutions and comparisons were made between smoke-free environment establishment as well as execution processes according to the fraction after converting into centesimal system (actual score/reserved portion × 100). Results The overall score for smoking control in Health and Family Planning System of Hubei Province has climbed to 79.29 ± 12.40.compared to that of 2011 has increased, but 1.6 lower than the national comprehensive evaluated score of 2014. The score of the city (82.14 ± 10.51) proved to be higher than that of the province (66.51±19.69) and the county, (75.35±13.40);disease control institutions (84.77± 9.45) enjoyed a higher score that the others with lowest scores in medical institutions (72.80 ± 14.95);Xianning won the highest score (88.52±5.12);Jingmen (69.81±5.16) and Xiaogan (67.92±7.98) proved to be the lowest in their scores. Nine regions in 17 embraced a lowest discrepancy of over 30 scores and the highest discrepancy of 50.94 scores between the highest score and the lowest among different institutions;12.73% (21/165) medical institutions had no publicity materials; 5/17 municipal institutions and 3/10 county level institutions were still found selling cigarettes, with only 46.06%(76/165) institutions reaching the standard of banning smoking in indoor places, 21.21%(35/165) institutions still have indoor smoking in non-working places, 1.82%(3/165) institutions have indoor smoking in working places. Only 18 (32.14%) institutions out of 56 medical institutions investigated have established smoking cessation clinics. Conclusions Banning-smoking work is less optimistic in general for Health and Family Planning System in Hubei Province, especially for substratum health and family planning institutions. All kinds of departments should take drastic measures to guaranttee the establishment of smoking-free environment.

Chlorine ; Computer Simulation ; Computers, Analog ; Diffusion ; Software

OBJECTIVE To know the disinfection and sterilization work quality in some hospitals, and to improve nosocomial disinfection and infection control management. METHODS Field selective examination and laboratory examination methods were used to carry out survey. RESULTS The eligible rate of indoor air was the lowest during three years in two provinces. The eligible rate of endoscope disinfection efficacy and ultraviolet radiation intensity was low. The eligible rate of autoclaving quality and oral instruments was more than 80% in the last two years. The eligible rate of sterile medical devices was over 90% in the last three years. CONCLUSIONS The results suggested that nosocomial infection incidence rate be decreased by improving disinfectant environment, adding disinfectant equipments or medical treatment instruments and enhancing doctors′ and nurses′ washing hands awareness.

Objective] To amplify,clone and express of a gene encoding hexose transporter of Plasmodium falcipuram(PfHT1) from Southern China isolate FCC1/HN for studing the immune of recombinant which protective from malaria parasite infection. [Methods] Cultivation of P.falciparum isolate FCC1/HN in vitro; extraction of genomic DNA from FCC1/HN using the alkali specific cleavage method; PCR amplification of PfHT1 and cloning into eukaryotic expression vector, pEGFPN3. The recombinant was introduced into mammalian cells, HEPG2 by using liposome\|mediated transfection. [Results] The gene encoding PfHT1 was specifically amplified from the genomic DNA of P.falciparum isolate FCC1/HN. The size of amplified fragment was 1 516 base pair. The eukaryotic expression recombinant, pN3\|HT1 , was constructed and expressed steadily in the hepatocarcinoma cell lines, HEPG2. [Conclusion] The gene encoding PfHT1 was successfully amplified and cloned. The pN3\|HT1/HEPG2 cell line was built for expressing fusion protein of GFP\|HT1.

To investigate the immunological activities of the recombinant human phosphatase D2 (rhPLD2) in vitro and in vivo, especially its ability to reduce inflammatory reactions, the cDNA fragment encoding rhPLD2 was cloned into prokaryotic expression vector pET30a by RT-PCR and the recombinant protein rhPLD2 expressed in E.coli was purified from the inclusion bodies, while the anti inflammatory activity of rhPLD2 was determined by the amount of eosinophils in bronchoalveolar fluid(BALF) and blood and the expression of IL-5 and MMP-9 in lung tissues of guinea pig model of chronic asthma. It was found that the rhPLD2 recombinant protein was obtained from human Daudi cells by cloning to E.coli, which contained no membrane-binding site and signal peptide. The cDNA sequence encoded 631 amino acid residues (GenBank Accession Number: AY178289). The purity of the rhPLD2 approached up to 76% with a bioactivity of 50.9745 units/L (0.9212 g/L). In addition, the anti inflammatory effect of rhPLD2 protein could be demonstrated in the guinea pig model of chronic asthma after treatment with rhPLD2 protein, such as down regulation in the expression of the inflammatory cytokine IL-5. It is concluded that the anti-inflammator activity of the recombinant human truncated PLD2 protein produced from the E.coli plasmid can be demonstrated both in vitro and in vivo.