2.A research on bond strength and interface of dentin adhesives
Ling YU ; Xiaoyan WANG ; Fucong TIAN ; Xuejun GAO
Journal of Practical Stomatology 2009;25(4):467-470
Objective: To evaluate bond strength and interface of various dentin adhesives by a modified interface processing method. Methods:Nine extracted human molars were ground to expose fresh dentin surface. Three dentin adhesives (Single Bond, Clearfil SE Bond and Clearfil S3 Bond) were applied respectively and a block of composite resin was built up. The specimens were then stored in distilled water for 24 h at 37 ℃ and sectioned into beams of 0.9 mm×0.9 mm cross-sectional area. The microtensile bond strength was determined with Microtensile Tester(n=15). In addition, the bonding specimens were conditioned with 6 mol/L HCl, 5% NaOCl and 0.08 mg/ml hyaluronidase to observe the adhesive interfaces by SEM. Results: Significant differences in bond strength were observed among the three adhesives(P<0.05): SE Bond (45.06±5.29) MPa> Single Bond (35.50±6.40) MPa> S3 Bond (30.46±3.82) MPa. Single Bond showed resin tags about 9-12 μm long and gaps between hybrid and adhesive layers. No obvious boundary between hybrid layer and adhesive were observed for both SE Bond and S3 Bond, while the length of resin tags were 9-14 μm and 4-7 μm for SE Bond and S3 Bond respectively. Conclusion:The morphology of the bond interface of dentin adhesive has a relationship with the bond strength.
3.Expression of cytokines Th1 and Th2 in patients with esophageal squamous cell carcinoma.
Peng-Cheng CHEN ; Jian-Guo FENG ; Yu-Tian LING
Chinese Journal of Oncology 2007;29(11):850-851
Adult
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Aged
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Aged, 80 and over
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Carcinoma, Squamous Cell
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immunology
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pathology
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Cytokines
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blood
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Esophageal Neoplasms
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immunology
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pathology
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Female
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Humans
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Interferon-gamma
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blood
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Interleukin-10
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blood
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Interleukin-12
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blood
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Interleukin-4
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blood
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Interleukin-5
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blood
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Lymphatic Metastasis
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Male
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Middle Aged
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Th1 Cells
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immunology
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Th2 Cells
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immunology
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Tumor Necrosis Factor-alpha
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blood
4.An analysis of correlation between post-stroke depression and relevant factors
Yali TIAN ; Chengyan LIN ; Ling GONG ; Cheng PANG ; Ping YU
Chinese Journal of General Practitioners 2002;0(02):-
Objective To investigate correlation between post-stroke depression (PSD) and multiple factors during onset of acute cerebral infarction. Methods Depression was measured with Hamilton Depression Rating Scales (HAMD) in 58 patients with acute cerebral infarction, and their neurological function were evaluated by neurological function defect (NFD) score. Their immunoglobulin G (IgG) index was calculated and level of nitric oxide (NO) in cerebrospinal fluid (CSF) was measured. Lesion and nature of cerebral infarction in 58 patients with acute stroke were located by CT. All the data were statistically analyzed with student-t test and ? 2 test, as well as linear regression model. Results Seventeen of 58 patients of stroke appeared PSD with an occurrence rate of 29.3%. Occurrence rate of PSD was significantly higher in patients with cerebral infarction than in those with cerebral hemorrhage 2=4.86, P
5.Research and Development of Health Maintenance Mobile Applications of Traditional Chinese Medicine
Ye TIAN ; Tong YU ; Qi YU ; Xiaofeng SUN ; Lili XU ; Ling ZHU ; Hongjie GAO ; Jinghua LI
Journal of Medical Informatics 2017;38(7):39-42
Based on the investigation and analysis of industry dynamics of health maintenance Applications (APP) of Traditional Chinese Medicine (TCM),the paper designs and develops health maintenance APP of TCM,and describes its architecture,operating principle,functions,innovativeness,applicability scope,etc.
7.Refractory Mycoplasma pneumoniae pneumonia combined with thrombosis:a case report and literature review
Yu CHEN ; Ping HUANG ; Weimin TIAN ; Ling CHEN ; Zhiqing LIN ; Qi CHEN
Journal of Clinical Pediatrics 2015;(2):121-125
Objective To investigate clinical features, diagnosis, and treatment of refractory Mycoplasma pneumoniae pneumonia (MPP) combined with thrombosis. Methods One case with refractory MPP associated with thrombosis was retrospectively analyzed with literature review. Results The patient presented with respiratory symptom at the onset, while melosalgia and decreased respiratory sound in left lung were occurred during anti-infection therapy. Thereafter, thrombosis of lower extremity veins and pulmonary embolism were confirmed by a series of examinations. Serum anti-phospholipid antibody was positive. Finally, the patient was treated with a combination of anticoagulation and immunosuppressive therapies. Conclusions The mechanism of refractory MPP combined with thrombosis may be associated with excessive inflammatory response and endothelial cells injury. The thrombosis complication should be suspected in patient of Mycoplasma pneumonia infection with positive anti-phospholipid antibody and low concentration of protein C and immunosuppressive therapy should be implemented promptly.
8.Doxorubicin induces enrichment of stem-like cells in osteosarcoma by activating Notch signaling
Ling YU ; Tian GAO ; Zhengpei ZHANG ; Chunjie TAO ; Weichun GUO ; Zhiwei FANG ; Zhengfu FAN
Chinese Journal of Clinical Oncology 2017;44(11):527-531
Objective:Cancer stem cells (CSCs) are resistant to chemotherapy. Our study aimed to investigate the stem cell-like proper-ties of doxorubicin-resistant osteosarcoma cell line 143B and its correlation with Notch signaling. Methods:We generated doxorubicin-resistant osteosarcoma cells by treating them with 2μm doxorubicin. Stem cell-like properties such as morphology change, Stro-1/CD117 double positive ratio, stem cell-related gene expression, sphere formation efficiency, and EMT character were assessed on day 5 after doxorubicin withdrawal. Notch receptor and its target genes were examined using qPCR and Western blot analysis. The stem cell-like properties of doxorubicin-resistant osteosarcoma cells were assessed when pretreated with Notch inhibitor or vehicle. The an-ti-tumor effect of Notch inhibitor was tested using a xenograft model. Results:Doxorubicin-resistant osteosarcoma cells were enriched in Stro-1+/CD117+cells, which showed obvious increased expression of stem cell-related genes, and exhibited enhanced spheroid for-mation and evident mesenchymal characteristics unlike doxorubicin-sensitive cells. qPCR and Western blot assays showed that Notch intracellular domain 1 (NICD1) and target genes Hes1 and Hey1 were upregulated in doxorubicin-resistant osteosarcoma stem cells compared with those in vehicle cells. Furthermore, pretreatment with a γ-secretase inhibitor (GSI) to prevent Notch signaling en-hanced chemo-sensitivity and inhibited doxorubicin-enriched osteosarcoma stem cell activity in vitro. Finally, the Notch inhibitor pre-vented tumor growth in mice xenograft models. Conclusion: Doxorubicin induced the enrichment of osteosarcoma stem-like cells through Notch signaling, and inactivation of Notch could be useful for overcoming drug resistance and eliminating osteosarcoma.
9.Research on genetic stability to American ginseng introduced into China for 30 years.
Xiao-Yu WEI ; Yi-Xin TIAN ; Zhi-Ling ZHAO ; Wei WEI
China Journal of Chinese Materia Medica 2014;39(19):3723-3726
To study the genetic stability of Panax quinquefolium after introduced into China for 30 years, the samples of P. quinquefolium from 14 regions of China were studied. RAPD molecular marker technology was applied in this research, and POPGEN32 data analysis and NTSYS2. 10 cluster diagram were used to analyze the data. The results showed that there are abundant genetic diversity in the ginseng samples. There were 81 polymorphic bands based on the 13 random primers. The polymorphism was 83.51%, the effective number of alleles (N(e)) was 1.456 7; Nei's gene diversity index (H) was 0.274 8; Shannon's diversity index (H(o)) was 0.419 4. The clustering analyses indicated that P. quinquefolium and P. ginseng were classified into two obvious groups, especially, it was also found that the P. quinquefolium could be divided into two obvious groups based on whether the P. ginseng was cultivated in the same region or not, but it was thought that there was not genetically a qualitative difference. Thus it suggests that a good breeding field should be established in Jilin Province of China for the germplasm purification.
China
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DNA, Plant
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genetics
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Genetic Variation
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Introduced Species
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Panax
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classification
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genetics
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Phylogeny
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Polymorphism, Genetic
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Random Amplified Polymorphic DNA Technique
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United States
10.The impact of bundles of care on complications and unplanned extubation rate of neonatal in PICC
Tian SHI ; Ling LI ; Xia CAO ; Ying LIU ; Renfeng LI ; Li HUANG ; Youxia YU
Chongqing Medicine 2014;(24):3189-3191
Objective To study the effects of bundles of care on complications and unplanned extubation rate of neonatal in PICC .Methods 116 cases of neonatal which didn′t accept the bundles of care ware group A ,the other 93 cases of neonatal which accepted the bundles of care ware group B .Two groups were observed and compared on the incidence of complications and un-planned extubation rate and so on .Results The complications in A group treatment were 70 cases(60 .3% ) ,while there were 25 ca-ses in group B(26 .9% ) ,the group B was significantly lower than the group A (P<0 .05) .Mechanical phlebitis ,catheter occlusion , catheter ectopic and other aspects of ectopic group B were significantly lower than in group A (P<0 .05) .The rate of unplanned ex-tubation and catheter blockage in group B were significantly lower than A (P<0 .05) .The total rate of ectopic and right basilic vein ectopic rate in group B were all significantly lower in group A (P<0 .05) .Group B unplanned extubation rate and catheter blockage weresignificantlyloweringroupA(P<0.05).Gestationalagedidn′thaveimpactoncomplications(P>0.05),birthweightand catheter days had more impact on neonatal complications (P<0 .05) .Conclusion The cluster management can reduce the incidence of neonatal PICC complications ,reduce unplanned extubation rates ,better play the role of PICC ,try to arrange an experienced staff of medical operations for children of low birth weight newborns .