Humans are exposed to the ubiquitous radiofrequency (RF, 100 kHz-300 GHz) electromagnetic fields because of the mushroom development of wireless communications,raising concerns over the possible hazards of RF radiations.Epidemiological investigation has showed that chronic use of cellphones increases the risk of brain tumors.Since genetic damage is closely related to tumors, researchers have been trying to find out whether cellphones and other RF devices are genotoxic.However, the investigations have yielded both negative and positive results.This review summarized the recent in vitro and in vivo researches about genotoxicity of RF radiations and proposed a possible mechanism by which of RF radiations cause genetic damage.

Objective To explore the relationship between some single nucleotide polymorphisms (SNP)loci of interferon regulatory factor 6(IRF6)gene,transforming growth faetor-?(TGFA)gene and nonsyndromic cleft lip with or without cleft palate(NSCL/P)in nuclear families consisting of fathers, mothers and affected offspring with NSCL/P from southeast China.Methods Some SNloci of IRF6 and TGFA were detected by applying microarray technology in nuclear families,and then haplotype relative risk (HRR)and transmission disequilibrium test(TDT)were performed.Results There were no significant difference in genotypes and alleles distribution between patients and their parents.The SNP locus——V274I of IRF6 was associated with NSCL/P(HRR:?~2=4.5816,P

Objective To explore the application of parents' presence induction program (PPIP) during anesthesia induction in children in the operation room and the nursing. Methods 86 children patients (1~11 years old) who would accept the operation were randomly divided into the observation and the control group (n=43 in each group). The observation group was accompanied by their parents when the children accepted the anesthesia induction under the instruction of nurses. But the patients were not present in the control group, and the patients were leaded into the operation room directly by the nurses to accept the anesthesia induction. Blood pressure and pulse changes before and after the anesthesia induction of these children patients were observed. The feeling of their heads of family before the operation and the satisfaction about PPIP were investigated. Results Blood pressure and pulses in the control group were significantly higher than those in the observation group before they were leaded into the operation room (P<0.01). 95% of heads of family hoped to accompany their children when they accepted the anesthesia induction, and 93.7% of heads of family wanted to take part in the PPIP. The satisfaction rate about PPIP was 95% after the completion of PPIP. Conclusions There are many profits about PPIP, such as mitigating the fear and anxiety of children and relaxing the unstable feeling of their heads of families before the anesthesia, and reducing the quantity of pre-anesthetic medications. These are helpful to the anesthesia induction and the cooperation of doctor patients. It is a best anesthesia induction mode worthy to be extended in the operation room.

Objective To investigate the effect of uvulopalatopharyngoplasty (UPPP) to obstructive sleep apnea hypopnea syndrome (OSAHS) in palients with resistant hypertension (RH).Methods UPPP and modified UPPP were performed on 36 moderate and severe OSAHS patients,who also suffered from RH [ who taking more than three kinds of antihypertensive drugs ( including diuretics ) and still not able to control blood pressure at 140/90 mm Hg (1 mm Hg =0.133 kPa)]. Polysomnography monitoring,ambulatory blood pressure monitoring,apnea hypopnea index ( AHI ),lowest saturation of blood oxygen ( SaO2 ) and the changes of antihypertensive medication dosage were performed to the patients before and 6 months after the surgery.Results Six months after surgery,the total efficiency was 61.1％ (22/36).The AHI median [ 25 quantile; 75 quantile] from 37.5 [26.0; 48.3 ]/h to 9.5 [9.0 ; 21.3 ]/h,the lowest the SaO2 average ( x ± s,the same below ) increased from 0.655 ± 0.114 to 0.860 ± 0.037,the differences were statistically significant (P value ＜ 0.05 ).24 h systolic blood pressure,daytime systolic blood pressure,night contraction,diastolic and mean arterial pressure decreased from ( 160.8 ± 6.8 ),( 170.5±2.5 ),(163.6±10.5),(100.8 ±5.6) and (96.8 ±7.5) mm Hg to (142.5 ±7.3),(150.8 ±7.6),(140.1 ±6.4),(81.8 ± 7.4) and (93.7 ± 2.4) mm Hg,the differences were statistically significant ( P ＜ 0.05 ).The degrees of AHI descent and the average SaO2 improvement were concerned with the degree of systolic blood pressure,diastolic blood pressure decent (r ＞0.80 and r2 ＞0.50).The average numbers of antihypertensive drugs decreased form ( 3.6 ± 0.5 ) to ( 2.9 ± 0.5 ) compared preoperatively and postoperatively,the difference was statistically significant (t =5.537,P ＜ 0.01 ). The use of antihypertensive medication reduced in 23 cases (23/36,63.8％ ) compared preoperatively and postoperatively.Conclusions The blood pressure of the patients with OSAHS and RH dropped significantly after UPPP surgery. Recent follow-up shows that the varieties of antihypertensive drugs taken in these patients are reduced significantly after operation.

Objective To investigate and compare the curative effect between delayed percutaneous coronary intervention (PCI) for patients with acute myocardial infarction presenting 12-24 hours from symptom onset and medical therapy on acute myocardial infarction patients presenting with ST-segment elevation (STEMI). Methods Using a prospective,open,parallel,controlled research approach,186 patients with STEMI were divided into delayed PCI group(n=89),which received PCI within 12-24 hours after STEMI and medical therapy group(n=97),which received medical therapy after STEMI. All patients were followed up 1-6 months with average follow-up (5.6 ± 1.4) months. Data of hospitalization period, the cardiac structures detected by echocardiography such as left atrial diameter (LAD), left ventricular diastolic diameter(LVDd),left ventricular ejection fraction LVEF,left ventricular fractional shortening(LVFS),composite end point events and major adverse cardiac events(MACE)were compared between the two groups.Results Compared with medical therapy group, the hospitalization cycle was significantly shorter in delayed PCI group. Data of the LAD and LVDd were significantly decreased,but LVEF and LVFS were increased in delayed PCI group compared with those of medical therapy group at 30 d and 6-month follow-up. The incidence of MACE and composite end point events were significantly less in delayed PCI group than those of medical therapy group (P<0.05). Conclusion Delayed PCI treatment can decrease the time of hospital stay and decrease the incidence rates of MACE and composite end point events,and improve left ventricular function and prognosis of patients.

OBJECTIVETo develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA).

METHODSA commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment. The sera of 55 fulvous fruit bats (Rousettus dasymallus) were tested using the SPA-ELISA.

RESULTSThe test results of the positive and negative controls in the kit and the serum samples from convalescent ;patient were consistent with expectation. Coronavirus antibody was detected in 2 out of the 55 bat serum samples. Serum neutralization test confirmed the validity of the SPA-ELISA method.

CONCLUSIONThis SPA-ELISA method is applicable for detecting coronavirus antibody in bat sera.

Animals ; Antibodies, Viral ; blood ; Chiroptera ; virology ; Coronavirus ; immunology ; isolation & purification ; Enzyme-Linked Immunosorbent Assay ; methods ; Immunoglobulin G ; blood

OBJECTIVETo evaluate the perinatal management and outcome of different types of fetal arrhythmia.

METHODSA retrospective analysis was conducted among the fetuses with arrhythmia identified by M-mode and pulsed Doppler echocardiography in a single institution between October 2003 and December 2010.

RESULTSA total of 130 fetuses were found to have fetal arrhythmia. The most common arrhythmia during pregnancy was extrasystole (n=59), followed by bradycardia (n=23), tachycardia (n=16), atrial flutter (AF, n=3), atrioventricular block (AVB, n=12) and other arrhythmia (n=17). The overall incidence of cardiac anomalies (commonly fetal bradycardia) was 9.2% in these cases. The prognosis of arrhythmia differed significantly between cases of different classifications. The type of fetal arrhythmia (P=0.024), presence of congenital heart defect (CHD, P=0.000) and fetal hydrops (P=0.008) were significant risk factors associated with termination of pregnancy.

CONCLUSIONFetal arrhythmias without CHD or hydrops under close monitoring often have good clinical outcome, while fetal bradycardia is associated with a high mortality rate. CHD and the presence of fetal hydrops are significant risk factors for pregnancy termination.

Adult ; Arrhythmias, Cardiac ; classification ; diagnostic imaging ; Female ; Fetal Diseases ; diagnostic imaging ; Heart Defects, Congenital ; diagnostic imaging ; Humans ; Pregnancy ; Pregnancy Trimester, Second ; Pregnancy Trimester, Third ; Retrospective Studies ; Ultrasonography, Doppler, Color ; Ultrasonography, Prenatal ; Young Adult

Objective To investigate the association between cutaneous adverse drug reactions (CADRs) caused by antiepileptic drugs and HLA-B*1502 allele. Methods In 31 epileptic patients presented to the Epilepsy Clinic of the Second Affiliated Hospital of Guangzhou Medical College between January 2007 and May 2008, 13 had CADR to carbanazepine (CBZ) including 6 with Stevens-Johnson syndrome (SJS) and 7 with mild maculopapular exanthona (MPE);15 were CBZ-tolerant, and 3 had lamotrigine (LTG)-indueed MPE. All the patients underwent examinations using polymerase chain reaction with sequence specific palmers to analyze HLA -B*1502 allele frequencies, with 30 healthy subjects without a history of using CBZ or LTG as the control. Results HLA-B*IS02 allele frequency was 100% (6/6) in patients with CBZ-SJS, 57% (4/7) in patients with CBZ-induced MPE, and 33% (1/3) in patients with LTG-induced MPE. The frequency was 7% (1/15) in CBZ-tolerant patients and 10% (3/30) in the control subjects. Compared with the CBZ-tolerant patients and the control subjects, the patients with CBZ-induced SJS and MPE had significantly increased HLA -B*1502 allele frequency (P<0.05). Conclusions HLA-B*1502 allele is associated with CADRs to CBZ in epileptic patients.

Objective To discuss the influence of intervention based o self-efficacy theory on the fluctuation of self-efficacy level in patients with nasopharyngeal carcinoma.Methods Two hundred and forty patients with nasopharyngeal carcinoma who were treated in radiotherapy department in the First Affiliated Hospital of Guangxi Medical University from Jun 2009 to Sept 2010 were chosen as the objective of this study.120 patients from Jun 2009 to Jan 2010 were the control group while 120 patients from Dec 2010 to Sept 2010 were the observation group.The control group was given conventional nursing while the observation group received intervention based on self-efficacy theory in addition.Patients' self-efficacy level was evaluated before,1-10 days after,11-20 days after and 21 days to the end of the treatment.Results The self-efficacy level was respectively(29.62 ± 3.20),(28.68 ± 2.65) and (29.25 ± 2.73) 1-10 days after,11-20 days after and 21 days to the end of the treatment in the observation group,better than (26.98 ± 3.16),(25.19 ± 3.62) and (24.28 ± 3.31) in the control group,and the differences were statistically significant (t =6.415,8.520,12.680,respectively; P ＜ 0.05).Self-efficacy level showed a descending trend in the control group during the process of tteatment,but first increased and then dropped but increased at last in the observation group.After intervention,patients' self-efficacy level was also different between two groups during different periods,and was better in the observation group than in the control group,with statistically significant differences (F =108.966,30.464,respectively; P ＜ 0.05).Conclusions Intervention based on self-efficacy theory can improve the self-efficacy level in patients with nasopharyngeal carcinoma and plays a positive role on the fluctuation of self-efficacy level in patients with nasopharyngeal carcinoma.

OBJECTIVETo investigate the prevalence rates of snoring disease and obstructive sleep apnea-hypopnea syndrome (OSAHS) and their risk factors among truck drivers in Guangzhou, China.

METHODSA questionnaire survey was conducted in 374 truck drivers who were selected from 5 logistics companies in Guangzhou by cluster sampling. Those who had potential snoring disease or OSAHS underwent polysomnographic monitoring at night. The obtained data were analyzed to calculate the prevalence rates of snoring disease and OSAHS and determine the risk factors for OSAHS.

RESULTSA total of 335 subjects completely questionnaires, with a response rate of 90%. Among the 335 subjects, 125 (37.3%) had habitual snoring, and 42 (12.5%) had OSAHS according to the diagnostic criterion (apnea-hypopnea index ≥ 5 times/h). The multiple stepwise regression analysis showed that the risk factors for OSAHS were age, alcohol use, family history of snoring, body mass index, and upper airway abnormality. Of the subjects with grade ≥ 2 snoring and OSAHS, 65.4% often felt sleepy when driving during daytime, and 42% had suffered or nearly suffered traffic accidents due to sleepiness when driving. Moreover, 95.5% (320) of the 335 truck drivers did not consider snoring a disease, and 98% did not think traffic accident might be related to snoring.

CONCLUSIONThe prevalence rates of snoring disease and OSAHS among truck drivers are 37.3% and 12.5%, respectively. Therefore, prevention measures should be established according to the epidemiological characteristics to help the truck drivers realize the hazards of snoring disease and OSAHS, thus minimizing the prevalence and hazards of the diseases.

Adult ; Automobile Driving ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Prevalence ; Risk Factors ; Sleep Apnea, Obstructive ; epidemiology ; Snoring ; epidemiology ; Surveys and Questionnaires ; Young Adult