1.STUDIES ON TEH UTILIZING THE PHOTOSYNTHETIC BACTERIUM FERMENTATION FOR BIOTRANSFORMATION ON CORN STRAW
Yu ZENG ; Si-Jun TAN ;
Microbiology 1992;0(06):-
The physiological character,functions and applications of photosynthetic bacterium had been discussed,during biotransformation of teh com straw treated with ammonia in aerobic,anaerobic and natural conditions,Comparing the concentration of teh reductive sugar and teh protein in teh fermented borth,we suggest a good way of biotransformating teh corm straw by photosyntehtic bacterium.In the photosynthetic bacterium fermentation in which the com straw treated substrate,teh concentrations of the reductive sugar and the transformative reductive sugar and teh protein in teh fermented borth were higher thean those without ammonia. Analysis of the results shows theat the transformative reductive sugar and protein were the nourishment of photosynthetic bacterium,so theere may be a way that we harness the corn straw by teh photosynthetic bacterium.
2.EFFECT OF EXOGENOUS GLUTAMATE ON COMPOUND ACTION POTENTIAL (CAP) AND COCHLEAR MICROPHONICS (CM) OF GUINEA PIG′S COCHLEA
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
To investigate the changes in CAP and CM recorded from the cochlear round window when glutamate solution in different concentrations(10mmol/L, 5mmol/L, 1mmol/L) was instilled into whole cochlea perilymph. The results demonstrated that there was a dependent correlation between CAP threshold shift and glutamate concentration. CAP threshold shift and amplitude could be markedly influenced by glutamate instillation. However, amplitude of CM was not obviously affected, and the I/O function of CM amplitude always maintained a nonlinear characteristic. The results suggest that glutamate has selective effects between inner hair cells and outer hair cells. High concentration glutamate might cause an increase in intracellular calcium, even as high as to induce toxicity. It could be induced by glutamate when it acts on IHC′s presynaptic autoreceptor in positive feedback manner, or on the afferent neurons, so that the synapsis is directly damaged by glutamate concentration overload in synaptic cleft.
4.The Reproduction of Nuclear Polyhedrosis Virus in the Midgut Epithelial Cell and the Other Sensitive Cell of Helicoverpa armigera
Yan-jun, ZHANG ; Jun, TAN ; Yu-qin, LIN
Virologica Sinica 2001;16(2):151-154
The reproduction of Helicoverpa armigera nucleopolyhedrovirus in the midgut epithelia cells and the other sensitive tissues was observed by electron microscopy. The reproducing viruses in the midgut epithelia cells were mostly without envelopes, and thte polyhedrons were seldom formed. The reproduciing viruses in the other sensitive cells were with envelopes, and packed in polyhedrons.
5.The efficacy of hypertonic saline treatment in cardiopulmonary resuscitation in animal model with cardiac arrest:a Meta-analysis
Wei LI ; Jun XU ; Dingyu TAN ; Xuezhong YU
Chinese Critical Care Medicine 2015;31(3):197-202
ObjectiveTo evaluate the efficacy of hypertonic saline (HS) treatment in cardiopulmonary resuscitation (CPR) in animal models of cardiac arrest (CA).Methods PubMed and EMBASE data were retrieved from January 1st, 1966 to September 30th, 2014, and Wanfang data and CNKI were searched from January 1st, 1990 to September 30th, 2014 for randomized controlled trials (RCTs) regarding CPR intervention of CA animal models with HS. HS was intravenously infused at the initiation of CPR in HS group, without limiting its dosage or concentration. The same volume of normal saline (NS) was given in NS group. Meta-analysis concerning the rate of restoration of spontaneous circulation (ROSC), the serum sodium concentration before CA and during CPR, and related hemodynamic parameters, including mean arterial pressure (MAP) and coronary perfusion pressure (CPP) at the immediate beginning of CPR and 90 minutes after ROSC was conducted by RevMan 5.3 software.Results A total of 8 RCTs were included. Meta-analysis showed that compared with NS group, the rate of ROSC [relative risk (RR) = 1.23, 95% confidence interval (95%CI) = 1.05-1.43,P = 0.010], serum sodium concentration during CPR [weight mean difference (WMD) =17.44, 95%CI = 12.57-22.31,P< 0.01], and the level of MAP at 90 minutes after ROSC (WMD = 4.81, 95%CI =1.58-8.03,P = 0.003) were significantly improved in HS group. There was no significant statistic difference in other hemodynamic parameters, including serum sodium concentration before CA (WMD = 0.78, 95%CI = -0.26-1.82,P =0.14), MAP (WMD = 5.43, 95%CI = -0.74-11.59,P = 0.08) and CPP at the immediate beginning of CPR (WMD =6.82, 95%CI = -5.54-19.19,P = 0.28), and CPP at 90 minutes after ROSC (WMD = -0.77, 95%CI = -10.33-8.80, P = 0.88) between two groups. It was showed by funnel chart that bias was not significant in the published articles. Conclusion This systematic review indicates that HS infusion is followed by an improved ROSC rate, serum sodium concentration during CPR, and MAP at 90 minutes after ROSC in animal models of CA.
6.Etiology and clinical features of acute pancreatitis in young patients
Wei TAN ; Hesheng LUO ; Jun YAN ; Yu CHEN ; Hong XIA
Chinese Journal of General Practitioners 2011;10(5):320-323
Objective To investigate etiology and clinical features of acute pancreatitis (AP) in young patients. Methods In total, 423 patients with AP admitted to Renmin Hospital of Wuhan University, Wuhan, Hubei province during January 2006 to December 2009 were retrospectively analyzed, 55 cases aged 34 years or less as young group and another 55 cases aged more than 34 years as middle- and elderly-age group. Their etiology, clinical features and illness scores based on some standard criteria were assessed. Results Proportion of varied etiologies of AP in young group was different from that in middle- and elderly-age one (x2 = 17. 107, P = 0. 009) , mainly as biliary and idiopathic pancreatitis in young group and as biliary and alcoholic pancreatitis in middle- and elderly-age one. Incidence of diet-related and idiopathic pancreatitis were higher in young group than that in middle- and elderly-age one ( x2 =4. 853, P = 0. 028 and x2=4. 274, P = 0. 039 ). Scores of the acute physiology and chronic health evaluation II ( APACHE II ) , Ranson criteria and the bedside index for severity in acute pancreatitis ( BISAP) were all lower in young group than those in middle- and elderly-age one (t = 5. 381 , P = 0. 000; t = 4. 388 , P = 0. 000 and t=3. 083, P = 0. 003 ) . There was no statistically significant difference in scores of computed tomography severity index ( CTSI) between young group and middle- and elderly-age one (t = ±0. 750, P > 0. 05 ) . Recurrence rate of AP was lower in young group than that in middle- and elderly-age one ( x2 =4. 251, P = 0. 039). Cholecystitis was more complicated in young group, pregnancy was more complicated in young group (x2 =4. 151 , P =0. 042) , and elevated blood glucose was more complicated in middle- and elderly-age one (x2 = 13. 285 , P = 0. 000 ) . Conclusions Etiology of AP varies in young patients with more dietary factors, occurrence of systemic complications and risks for death are lower in young group than those in middle- and elderly-age one, and there was no statistically significant difference in local complications and severity of illness between the two groups.
7.Relationship between subjective well-being and family care degree of medical college students
Yulan YU ; Jianfeng TAN ; Hezhan LI ; Jun LUO ; Chonghua WAN
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(1):66-68
ObjectiveTo study the relationship between subjective well-being and family care degree of medical college students.Methods2695 medical college students were tested by Family APGAR index and Oxford Happiness Test-Revised.The data was analyzed by independent-sample t test,pearson correlations and linear regression.Results ( 1 ) The subjective well-being and family care degree and its factors of college students were above average.The score of subjective well-being was 43.08 ± 11.16,and the score of family care degree was 6.48 ±2.39.(2)The family care degree( t=3.462,P<0.01) and its adaptation( t=2.718,P<0.01),partnership( t =4.178,P<0.01),and growth( t=4.484,P<0.01 ) of female students were higher than male students.The family care degree ( t =3.939,P < 0.01 )and its adaptation ( t =4.683,P < 0.01 ),partner-ship ( t =3.306,P< 0.01 ),and growth ( t =2.796,P < 0.01 ) and subjective well-being( t =3.508,P< 0.01 ) of the students who came from cities and towns were higher than the students who came from rural.(3)There was a significant relation between subjective well-being and family care degree and its factors( r=0.197 ~ 0.357,all P<0.05 ).The partner-ship,resolve,growth and affection can forecast together the 13.2% variance of subjective well-being.ConclusionEnhancing family care degree is contribute to the subjective well-being and psychological health of medical college students.
8.Effect of Strengthening the Spleen and Tonifying the Kidney on the Function and Apoptosis of Kidney in Rats after Exhaustive Exercise
Xiang-Mei LIU ; Jun TAN ; Yu-Qiang WU ;
Journal of Traditional Chinese Medicine 1992;0(12):-
Objective To study the effect and mechanism of strengthening the spleen and tonifying the kidney on the function and apoptosis of kidney in rats.Methods The 42 male SD rats were randomly divided into quiet control group,exhausted exercise group and strengthening the spleen and tonifying the kidney group.Twenty four hours after exhaustive exercise,the concentrations of blood urea nitrogen(BUN),serum creatinine and urinary protein were determined.The apoptosis in kidneys of rats was detected by TUNEL.The structure of kidney was observed in the HE stain.Results Comparing with the exhausted exercise group,the concen- trations of BUN,serum creatinine and apoptotic index were lower significantly(P
9.Determination of 9 Phenoxy Acid Herbicides in Soil by Solid Phase Extraction and LC/MS/MS
Yanbin YU ; Li LI ; Jun YANG ; Pigong TAN ; Zongxing LIU
Chinese Journal of Analytical Chemistry 2014;(9):1354-1358
A method for the determination of 9 phenoxy acid herbicides in soil by solid phase extraction and LC/MS/MS with matrix correction was developed. The sample was treated by ultrasonic extraction twice with 1. 5% formic acid in acetonitrile. The extract was cleaned up with NH2 solid phase extraction column. A C18 column with formic acid in pure water/methanol mixture as the mobile phase was used under gradient elution. Mass spectral acquisition was done in negative ion mode by multiple reactions monitoring ( MRM ) . Two transitions per compound were used to provide qualitative and quantitative analysis. There were good linear relationships in the range of 2-250 μg/L for 9 phenoxy acid herbicides. The correlation coefficients were in the range of 0 . 9927-0 . 9972 . The method detection limits were in the range of 1 . 66-3 . 82 μg/kg . The average recoveries at three spiked levels ( 10 , 40 , 200 μg/kg ) ranged from 85 . 3% to 110 . 0% with the relative standard deviations (RSD) of 3. 2%-12%.
10.Advances of ventilation during cardiopulmonary resuscitation
Dingyu TAN ; Yangyang FU ; Jun XU ; Xuezhong YU
Chinese Critical Care Medicine 2016;28(7):661-665
As one of the cornerstones of modern cardiopulmonary resuscitation (CPR), ventilation received controversy and challenges in the past two decades. From 2000 to 2015, the changes in CPR guidelines of American Heart Association (AHA) showed that the position of ventilation declined gradually as compared to chest compressions. Chest compressions only CPR has been strongly advocated in recent years, especially in witnessed cardiogenic cardiac arrest (CA). Passive oxygenation and cardiocerebral resuscitation (CCR) also showed good effect in the early stage of cardiogenic CA. However, clinical validation in a larger context is still needed. An impedance threshold device (ITD) transiently blocks air from entering the lungs during recoil, decreases the intrathoracic pressure, facilitates venous return to the chest and increases coronary blood flow. However, the relevant research findings are not consistent, and the guidelines do not recommend routine use of ITD. Positive-pressure ventilation, which can increases intrathoracic pressure, affects the coronary perfusion pressure (CPP) and cerebral perfusion, is thought to be not only useless, but also has adverse effects within the first few minutes of CPR. This view is accepted by many scholars, however, ventilation is essential in late-start CPR, prolonged CPR and non-cardiogenic CA. Mechanical ventilation, especially special ventilation modes for CPR showed some prospects. Positive-pressure ventilation remains the gold standard in CPR in clinical practice at present. It was shown by existing research that hyperventilation significantly reduce the success rate of resuscitation, thus a consensus had been reached about avoiding hyperventilation. Currently, the number of studies on ventilation during CPR is very limited, and many of the conclusions are not consistent among studies. Therefore, more high-quality studies are needed in future to further clarify the application of ventilation during CPR.