[Objective] To investigate the role of exenatide on angiogenesis in endometrial cancer Ishikawa xenografts in nude mice.[Method] We used the subcutaneous human endometrial cancer cell Ishikawa xenografts in nude mice model,and divided them into control group and exenatide-treated group.The harvested tumors were preserved for immunohistochemistry staining and western blot analysis.[Results] The micro-vessel density (CD31 positive) in exenatide-treated group was (13.2 ± 1.4)/400 power field,less than that in control group [(25.9 ± 5.8)/400 power field] (P ＜ 0.01).The expression level of VEGF was significantly lower in exenatide-treated group than that in control group (P ＜ 0.05).The mean density of tumor associated macrophages (F4/80 positive) is (31.4 ± 3.4)％ in exenatide-treated group and (72.1 ± 4.2)％ in control group with significant difference (P ＜ 0.01).[Conclusion] Exenatide weaks tumor angiogenesis within endometrial cancer Ishikawa xenografts in nude mice.

Phytoestrogen is a kind of plant element which is similar to animal estrogen. Phytoestrogens can prevent progreator system cancer, cardiovascular disease and osteoporosis. Recently more and more researches are reported on the protection of phytoestrogens on central nerve system.

AIM To investigate the effects of ondansetron, a selective 5 Hydroxytryptamine3 (5 HT 3) receptor antagonist, on morphine physical dependence. METHODS The morphine dependent models in mice and in isolated Guinea pig ileum were used. RESULTS Pretreatment of ondansetron for 12 days significantly reduced morphine withdrawal symptoms in mice ,such as body weight loss(Groups 2～100 ?g?kg -1 ?d -1 ) or reduced both body weight loss and jumping times (Group 100 ?g?kg -1 ?d -1 ). In addition, concomitant treatment with ondansetron(1～20 ?mol?L -1 ) dose dependently suppressed the contraction induced by naloxone in Guinea pig ileum. CONCLUSION The chronic pretreatment of ondansetron can prevent morphine physical dependence to some extent.

Objective To investigate the effects of ultrashortwave (USW) diathermy and electrical stimulation (ES) used in combination with nerve growth factor (NGF) in the treatment of experimental sciatic nerve injury.Methods Sixty adult Wistar rats were randomly divided into a normal control group, a model group, an NGF group,a physiotherapy group and a combined treatment group. A model of sciatic nerve injury was established in the latter four groups. Beginning on the 2nd day after the operation, no treatment was given in the model group, NGF was injected in the NGF group, diathermy and ES were administrated to rats in the physiotherapy group, and the combined treatment group was treated with USW diathermy, EW and NGF. Function, electrophysiology and morphology were evaluated at the 2nd, 7th, 14th and 30th days after the operation Results The average sciatic nerve function index (SFI), nerve conduction velocity (NCV) and nerve regeneration in the NGF, physiotherapy and combined treatment groups were significantly better than in the model group, with those in the combined treatment group improved to the greatest extent. At the 30th day there was no significant difference between the combined treatment group and the normal control group in terms of SFI, NCV, axon regeneration or myelin sheath thickness. The number of myelinated nerve fibers and the average axon diameter in the combined treatment group and normal control group were significantly higher than those in the model, NGF or physiotherapy group. Conclusions With NGF injection, additional application of USW diathermy and ES may significantly enhance the regeneration of the sciatic nerve and aid functional recovery after injury.

97 cases of jaw bone fractures were treated with rigid internal fixation.16 cases had malocclusion postoperation.The etiology was analyzed and the prevention methods were proposed.Intermaxillary elastic traction or fixation and craniomaxillofacial fixation or craniomentum elastic traction were performed to treat the malocclusion.By this way,14 cases were cured.1 case was operated again to resume the normal occlusion,1 case received occlusal adjustment.Incomplete reduction,incorrect operative performance,deficiency of postoperative intermaxillary fixation and incorrect intermaxillary fixation were the main causes to malocclusion.So anatomic in time reduction,correct operative performance,intermaxillary fixation,application of craniomaxillofacial fixation or craniomentum elastic traction instead of intermaxillary fixation for some special cases are effective methods to correct postoperative malocclusion.

Objective:To observe the change of CD4 + T cells of SARS patients in early stage of disease and determine its clinical significance on the progress of disease and therapy selection.Methods:Detection the absolute counts of T cell subset from the peripheral blood samples of 52 SARS patients in initial 10 days' duration by Flow cytometry.Serial frontal chest radiographs had done and the progression of disease was observed during the treatment of all patients.Results:The 34 cases among the 52 patients were in normal range of CD4 + cell absolute count.At the same time,their pulmonary lesions were in limited degree and the states of an illness were stabilization and the pulmonary lesions were absorbed quickly.Therefore they reuired the treatment without corticosteroids.The other 18 patients were with low CD4 + cell absolute count, of which 13 cases showed progressive deterioation of radiographic change and were treated with corticosteroids additional.The other 5 cases with the mild low of absolute count of CD4 + cells were also static during the period of observation,and no treatment with coticosteroids.There was statistically significant relation between two groups(P=0 000).The results of Pearson correlate analysis between absolute count of CD4 + cells and pulmonary lesions were r=-0 737;P=0 000;The result between absolute count of CD4 + cells and corticosteroids treatment were r=-0 573;P=0 000.Conclusion:Peripheral blood CD4 + cell absolute counts of SARS patients on early stage of onset were negatively related to the degree of pulmonary lesions.The patients with remarkable decrease of CD4 + cell were in need of treatment with corticosteroids.

Objective To study the construction of the lentiviral-mediated RNA interference(RNAi) vector targering rat suppressors of cytokine signaling 3(SOCS3) gene.Methods Three target sequences were selected by on-line designer software on Ambion according to rat SOCS3 mRNA sequence(NM053565),the complementary DNA contained both sense and antisense oligonucleotides were designed and synthesized.After annealing,these double strands DNA were cloned to pRNA-Lenti-green fluorescent protein(GFP),which contained U6 promoter and GFP.The resulting Lentiviral vector containing SOCS3 shRNA was named pRNA-Lenti-SOCS3-GFP.After the rat glioma cells(C6)were transduced with the constructed 1entiviral vectors,real-time polymerase chain reaction was used to evaluate the level of SOCS3 expression(including siRNA1 group,siRNA2 group,siRNA3 group,vacuity group and siRNA-Negative group).The pRNA-Lenti-SOCS3-GFP and Lentivector Pakaging plasmid mix were cotransfected into 293T to package Lentivirus particles.Culture supematant was harvested,then the virus titer was determined by serial dilution assay.Results The SOCS3 mRNA sequence was successfully cloned to pRNA-Lenti-GFP,which was proved by PCR and DNA sequence.Compared with control group,the SOCS3 mRNA expressions were obviously suppressed in all 3 experimental groups,especially the expression rate in siRNA1 group was reduced by 80%.The Lentiviral particle titer was determined by serial dilution assay with 1.0?1010 TU?L-1.Conclusion The lentiviral-mediated RNAi vector of rat SOCS3 gene has been constructed successfully,this may provide a potential tool for studying and treating SOCS3-related diseases.

Objective To study the effects of Forkhead box protein M1 (FoxM1) down regulation by small interfering RNA(siRNA) on chemosensitivity and mechanism of human pancreatic cancer cell and its mechanism.Methods Three FoxM1 siRNAs were designed and constructed.All cancer cells were divided into different groups,after transfected with FoxM1 siRNA for different time,the cultured cells were harvested to carry on the next tests.Expression of FoxM1 were determined by red-time PCR and Western blot,and prolifearion and chemosensitivity were evaluated by MTT assay,and the phosphorylation of Akt protein was examined by Western blot.Results FoxM1 siRNA could down-regulate the FoxM1 expression in a dose-and time-dependent manner.The MTF results showed that the inhibit rates was 17.78％,17.56％,35.39％,52.81％,70.98％ indifferentgroups [Con-A + Gemcitabine,Con-B + Gemcitabine,siRNA (3.125nM) + Gemcitabine,siRNA (6.25nM) + Gemcitabine and siRNA(12.5nM) + Gemcitabine,respectively.The phosphorylation of Akt protein was inhibited in a dose-dependent manner.Conclusions FoxM1 siRNA could sensitize human pancreaticr cancer cells chemotherapy sensitivity,it is the one of the important mechanisms through down-regulate Akt phosphorylated levels,but the molecular mechanism need to be explored further.

Aim To investigate the protective effect of zileuton,a 5-lipoxygenase inhibitor,on focal cerebral ischemia-reperfusion injury in rats.Methods The right middle cerebral artery of the rat was occluded by inserting a thread through internal carotid artery for 2 h,and then reperfused for 24 h.Zileuton(10,50 mg?kg~(-1)) was orally administered 2 h before ischemia and 0,5,10 h after reperfusion.After 24 h reperfusion,the content of malondialdehyde(MDA) and nitric oxide(NO),the activities of glutathion peroxidase(GSH-PX),myeloperoxidase(MPO) and nitricoxide synthase(NOS) were measured.Results Compared with vehicle group,the infarct size,content of NO and NOS of the brain were significantly reduced in 10 and 50 mg?kg~(-1) zileuton groups;zileuton 50 mg?kg~(-1) also reduced the content of MDA,increased the activity of GSH-PX,and inhibited the increase of MPO in brain tissue.Conclusion Zileuton possesses the neuroprotective effects on focal cerebral ischemia-reperfusion injury in rats.

The JC virus is a widely infected human polyomavirus. Recent foreign researches showed that the JC virus infection is correlated with tumors of nervous system and digestive system, while, and study on the relationship between JC virus infection and gynecological tumor is seldom reported. In this study, we first establish the nucleic acid detection methods and procedures for JC virus and its highly homologous BK virus. The JC and BK viruses infection was evaluated by detect the viral DNA in samples including biopsy tissues, serum as well as urine of myoma of uterus (98 cases), cervical cancer (84 cases), endometrial cancer (40 cases) and ovarian tumor (72 cases) patients. The BK viral DNA positive rate was significantly higher in urine samples than that of blood and biopsy samples, and there is no significant difference of the BK viral DNA positive rate among all patient groups. The JC viral DNA positive rate is almost 0 in serum samples and biopsy. tissues, however, viral DNA positive rate is more than 50% in urine samples. In fibroids group, the JC viral DNA positive rate is up to 65. 3% which is significantly higher than that in other patients groups and healthy control. Further gynecological tumor associated viruses detection showed that only human papilloma virus infection is associated with cervical cancer, the herpes simplex virus, EB virus and cytomegalovirus infection is extremely low in our patient groups. No synergistic effect on gynecological tumor caused by viruses co-infection was observed. Our study showed that JC virus infection is highly related to the pathogenesis of uterine fibroids.
Adult ; Female ; Genital Neoplasms, Female ; virology ; Humans ; JC Virus ; classification ; genetics ; isolation & purification ; Middle Aged ; Polyomavirus Infections ; virology ; Tumor Virus Infections ; virology ; Young Adult