As a clinical diagnosis and treatment of low back pain,straight leg raising test has been used most commonly more than 100 years.It is simple and practical,and has a high clinical value.As a very mature orthopedic special examination,straight leg raising test progress in non-orthopedic applications more often than orthopedic aspects.Of course,the most important thing is the diagnosis of low back pain,followed by the treatment of low back pain,and then can be used as a respiratory and circulatory aspect of diagnosis and treatment options.This article elaborated the principle of straight leg raising test and the clinical research progress in order to give the clinician some guidance.

Objective To assess the safety of living environment by the elderly, further clarify the relevant factors of the elderly fall in the living environment. Methods The self- made assessment form of fall-related factors of living environment for elderly patients was used to survey 1 076 elderly. Likert five grades score was used for each item, and the higher score demonstrated the safer living environment. Results In the assessment of the safety of living environment, the indoor physical environment (3.94 points), home furniture safety (3.66 points) score was better;home ground and the channel (3.32 points) and outdoor activities places (3.57 points) score was lower. Scores of home′s ground and channel, furniture safety, indoor physical environment and living environment of urban elderly were (23.56 ± 6.49), (44.52 ± 7.72), (11.93 ± 2.49), (105.03 ± 17.82) points, which were better than (22.46 ± 6.39), (42.73 ± 7.95), (11.53 ± 2.39), (101.55 ± 18.15) points of rural elderly (t=2.471-3.495, P <0.05). Conclusions The indoor physical environment, home furniture safety got higher scores, and the scores of ground-channel environment and outdoor activities places were relatively lower. Scores of the safety of living environment in urban are better than in rural. It suggests the elderly living environment need to reduce the incidence of falls.

Objective To analyze the effect of modified Yulin Decoction on hormones and ovarian function in patients with premature ovarian failure (POF).Methods 142 cases of patients with POF and kidney-yang deficiency syndrome were selected and randomLy divided into the control group (n=64) and the observation group (n=78).Levels of serum hormones and ovarian function,the traditional Chinese medicine (TCM) symptom score in the two groups were observed before and after treatment.Results Before treatment, there were no significant differences between the two groups in the scores and levels of hormones.After treatment, levels of serum FSH, LH and E2 were significantly decreased in the two groups, and the changes were more obvious in the observation group than the control group (P<0.05).After treatment, TCM symptom scores of two groups were significantly decreased, and the observation group were significantly lower than those in the control group (P<0.05).Conclusion Modified Yulin Decoction is significantly effective in the treatment of POF of spleen and kidney-yang deficiency syndrome.It can significantly improve the level of estrogen, adjust endocrine and relieve clinical symptoms.

OBJECTIVETo investigate the effect of 8-aminolevulinic acid (ALA) photodynamic therapy (PDT) on the cell proliferation, apoptosis and collagen secretion in keloid fibroblasts and to provide the theoretical base for ALA-PDT treatment of keloids.

METHODSFibroblasts from keloid patients were cultured to the third generation in vitro and incubated in 0, 1, 3, 6, 9 mmol/L of δ-aminolevulinic acid for 3 h in the darkness. Then they were exposed to 635 nm wavelength red light ( 30 J/cm2 ) and continued incubation 24 h after irradiation. CCK-8 assay was used to detect proliferation inhibition rate of fibroblasts. The content of hydroxyproline was measured by colorimetric method. The expression of p-Akt and programmed cell death 4 ( PDCD4) were detected by Western blot.

RESULTSThe inhibition rate of keloid fibroblasts were respectively 0, (8.30 ± 1.01)%, (29.48 ± 3.27)%, (52.01 ± 5.34)%, (79.99 ± 5.85)% with the presence of difference concentrations (0, 1, 3, 6, 9 mmol/L) of ALA. The content of hydroxyproline were respectively (9.540 0 ± 0.352 42), (6.242 5 ± 0.224 85 ), (5.107 5 ± 0.534 88), (3.490 0 ± 0.623 48), (2.945 0 ± 0.514 10) μg/mg. The relative expression of p-Akt were respectively 1, 0.75 ± 0.12, 0.52 ± 0.14, 0.41 ± 0.18, 0.32 ± 0.09. The relative expression of PDCD4 were respectively 1, 1.18 ± 0.19, 1.51 ± 0.22, 0.15 ± 0.30, 2.44 ± 0.22. The difference was statistically significant when compared the group of 1, 3, 6, 9 mmol/L with 0 mmol/L (P < 0.05).

CONCLUSIONSIn concentration within the range of 1-9 mmol/L, ALA could inhibit the proliferation of fibroblasts significantly, promote fibroblasts apoptosis and reduce the content of hydroxyproline in a dose-dependent manner, indicating that 8-aminolevulinic acid photodynamic therapy may be a potential treatment for keloid.

Aminolevulinic Acid ; pharmacology ; Apoptosis ; drug effects ; Cell Culture Techniques ; Cell Proliferation ; drug effects ; Collagen ; secretion ; Fibroblasts ; cytology ; drug effects ; secretion ; Humans ; Keloid ; drug therapy ; pathology ; Light ; Photochemotherapy ; methods ; Photosensitizing Agents ; pharmacology

OBJECTIVE: To prepare indapamide sustained release capsules and investigate its release in vitro. METHODS: Blank pellets, hydrated magnesium silicate, hypromellose cellulose, triethyl citrate, Eudragit RL 100, Eudragit RS 100, Eudragit LS 55, etc, were used as materials to prepare indapamide sustained release capsules The content was determined by HPLC, and the effects of different solid weight gains of sustained release material on release of indapamide from capsules were evaluated by in vitro release test. RESULTS: The release in vitro of the sustained release pills met the requirements when the solid weight gain of the materials was 5.0% to 5.3%. CONCLUSION: The optimum preparation technique of indapamide sustained release capsules is established, which has guiding significance for the practical manufacture.

Autophagy is an important homeostatic cellular recycling mechanism responsible for degrading injured or dysfunctional cellular organelles and proteins in all living cells. Aging is a universal phenomenon characterized by progressive deterioration of cells and organs due to accumulation of macromolecular and organelle damage. Growing evidences indicate that the rate of autophagosome formation and maturation and the efficiency of autophagosome/lysosome fusion decline with age. Dysfunctional autophagy has also been observed in age-related diseases. Autophagy disruption resulted accumulation of mutated or misfolded proteins is the essential feature of neurodegenerative disorders. However, in cancers, fibroproliferative diseases or cardiovascular diseases, autophagy can play either a protective or destructive role in different types of disease, and even in different stages of the same disease. The review will discuss the cellular and molecular mechanisms of autophagy and its important role in the pathogenesis of aging and age-related diseases, and the ongoing drug discovery strategies for therapeutic intervention.
Aging ; Autophagy ; Drug Discovery ; Humans ; Lysosomes ; metabolism ; Neurodegenerative Diseases ; Phagosomes ; metabolism ; Protein Folding

OBJECTIVE: To investigate the influence of ultraviolet B (UV-B) induction on the content of two kinds of secondary metabolites in mulberry leaves and differential proteomics under UV-B induction. METHODS: The HPLC chromatograms of secondary metabolites in leaves before and after UV-B induction were established and quantitative analysis of two kinds of secondary metabolites was performed. The subcellular structure of mulberry leaves under UV-B induction was observed using transmission electron microscopy. Proteomic analysis of mulberry leaves was performed using two-dimensonal electrophoresis (2-DE). RESULTS: The subcellular structure of mulberry leaves was destroyed under UV-B induction. The contents of moracin N and chalcomoracin in mulberry leaves were increased with the dark incubation time and they maximized at 36 and 40 h, respectively. The result of proteomics showed that the abundance of chalcone synthase (CHS) which is the key enzyme of flavonoid biosynthesis pathway was increased significantly. CONCLUSION: The secondary metabolism especially for flavonoids biosynthesis in mulberry leaves is changed under UV-B induction and dark incubation, which result in the increased moracin N and chalcomarcin.

Objective To investigate the influence of all-round intervention on the quality of life and the emotion of cancer patients who have accepted the gamma knife therapy. Method Using health education, psychotherapy and the behavior treatment for 260 patients with malignant tumor who had accepted the gamma knife therapy. Evaluated the quality of life and the emotion of these patients before and after the intervention respectively by using questionnaire of QOL, SCL-90, SDS and SAS. Results The values of indicated the QOL, SCL-90, SDS and SAS before the all-round intervention were 23.12?4.68, 68.12?33.26, 40.12?10.28 and 42.48?12.16 respectively. The values which had obtained after the all-round intervention were 46.26?3.28, 36.12?26.23, 28.16?5.23 and 26.12 ?10.66 respectively, there were significant differences between them,P

Objective To investigate the clinical efficacy of endovascular treatment for iliac vein compression syndrome (IVCS) in 112 cases. Methods From September 2011 to June 2015, 112 cases of IVCS (Cockett syndrome) were treated in our hospital with endovascular methods .The case numbers of different CEAP ( clinical etiological anatomical pathophysical ) classifications were C2 (45 cases), C3 (23 cases), C4 (19 cases), C5 (11 cases) and C6 (14 cases).All the cases were diagnosed by venography .The operations started from puncturing the ipsilateral veins .The stenotic degree and compressed intensity of iliac vein were evaluated by the minimum diameter with low pressure balloon .And then a stent was placed into after percutaneous transluminal angioplasty ( PTA) when residual stenosis was more than 50%.The occlusive segment was dilated by 8 mm diameter balloon firstly , and catheter directed thrombolysis treatment was applied if thrombus was detected .The PTA and stenting were performed when the thrombus was completely removed .The improvement of clinical symptoms was observed after operation and the patients were followed up by ultrasound and venograghy . Results Nine cases were given PTA only , and the other 103 cases underwent stent placement . Postoperative venography showed collateral vessels were disappeared in 22 cases, obviously decreased in 56 cases and mildly decreased in 34 cases.The average difference of swelling leg ’s circle was diminished from (2.56 ±0.88) cm pre-operation to (0.93 ±0.71) cm post-operation, while the average area of skin ulcer was diminished from (6.34 ±3.78) cm2 pre-operation to (2.13 ±1.88) cm2 post-operation, all of which were statistically significant (t =20.24 and 7.19,P=0.000).A total of 86 cases were successfully followed up during a period from 2 months to 45 months (average, 19.2 months).The patency rate of treated iliac vein 12 months after operation was 76.2%in the PTA group, and in the PTA with stenting group was 96.7%, 88.6%, and 86.1%, respectively, at 12, 24, and 36 months after operation, in which a statistical significance was detected (log-rank χ2 =30.32, P=0.000).The patency rate in diameter ≥16 mm group was 95.2%and 95.2%, respectively, at 12 and 24 months after operation, while in diameter <16 mm group was 97.0%and 93.3%, respectively, in which no statistical significance was detected (log-rankχ2 =0.39, P=0.532).Thrombotic complication was found in 2 cases during the follow-up period , including 1 case of iliac vein thrombosis at 1 month and 1 case of thrombus in contralateral leg 8 months later .According to the results of venography in 78 cases at 12 months after operation , restenosis was found in 5 out of 6 cases in the PTA group, and the patency rate in the stent placement group was 95.8%(69/72).No stent migration was found . Conclusion Comfortable clinical results were obtained after endovascular therapy for iliac vein compression syndrome , and the application of stenting could increase the middle and long term patency rate of treated vessels .

Humans ; Infant, Newborn ; Sepsis ; diagnosis