1.The evaluation of educational measurement indexes from the Undergraduates Clinical Skills Competition in a medical university
He YANG ; Xiaosong YU ; Hua WEN
Chinese Journal of Medical Education Research 2015;14(4):345-348
Objective The purpose of this study was to analyze and evaluate the educational measurement indexes from the undergraduates clinical skills competition in a medical university.The rationality of the competition was discussed in the view of education survey.Methods 84 students' results which selected from the China Medical University 2nd Clinical Skills Competition were the research objects in this study.The indexes of the difficulty,discrimination,reliability and criterion-reated validity of the competition were evaluated based on the Education surveying principle.The difficulty was calculated by subjective topic difficulty computing formula,and other indexes were carried out with SPSS 18.0 except the difficulty index.Results The average difficulty of the questions of this university was 0.61,the average discrimination was 0.77 and the overall reliability was 0.926.The OSCE scores of the participating students were taken as the criterion in this study.The calculated criterion-related validity was 0.416.Conclusion The college students' clinical skills competition has good reliability,discrimination and effective criterion-related validity.For the selective competition,the difficulty can be further improved while considering the scope of the topic.
3.Rapid Determination of Long-chain Aliphatic Aldehyde in Gutter Oil by Fluorescent Derivatization-High Performance Liquid Chromatography
Wan WANG ; Qingfeng YU ; Ying XIAO ; Hua HE
Chinese Journal of Analytical Chemistry 2017;45(5):770-776
Reuse of waste oil in cooking has been the social concern for long time, but until now there is no reliable method to identify the gutter oil.In this work, based on the generation of long-chain aliphatic aldehydes in the refinement of gutter oil, gutter oil and adulterate cooking oil were identified by determining hexanal, heptanal, octanal, nonanal and decanal in oil sample with high perfomance liquid chromatography (HPLC) method using O-(3-(9H-carbazol-9-yl)propyl)hydroxylamine as the fluorescent derivative reagent.At first, 10 μL of oil sample was dissolved in 200 μL of isopropanol, then reacted with O-(3-(9H-carbazol-9-yl)propyl)hydroxylamine to form the stable derivatives, extracted with acetonitrile and injected into HPLC for analysis.The derivatives were separated on a C18 column within 15 min with ACN-H2O (90∶10, V/V) as mobile phase.The fluorescence detector was set at λex/λem=292 nm/348 nm.The linear range of 5 kinds of long-chain aliphatic aldehydes was 0.01-1.00 μmol/L with limit of detection (LOD, S/N=3) of 0.05-0.10 nmol/L, and the spiked recoveries were 95.6%-101.4%.The results showed that the edible oils obtained from supermarket almost had no hexanal, heptanal, octanal, nonanal, decanal, but these aldehydes increased significantly in gutter oil.Thus the 5 kinds of long-chain aldehydes could be used as the indictor of gutter oil.Taking the advantage of good specificity, higher sensitivity, good accuracy and simplicity, the method was suitable for the rapid identification of gutter oil.
4.Adaptive Reaction of Actin Cytoskeleton in Podocyte Structure of Glomerulus
yu-lin, KANG ; guang-hua, ZHU ; wei-xun, HE
Journal of Applied Clinical Pediatrics 1992;0(05):-
Actin cytoskeleton in podocyte is a complicated network structure,and the stability of this structure depend on many proteins which located in slit diaphragm,the apical membrane domain and the basal membrane domain with the stimulus of mechanical stress,the actin cytoskeleton can be adaptive regulated to maintain the normal function of glomerulus,and several signal pathways involve in the process,such as RhoA/Rho kinase signal pathway and TRPC6.
5.Practice and experience of postoperative complications reporting system
Shengdong PAN ; Hua YU ; Xiaohua HE ; Jianshan MAO ; Shaowen XU
Chinese Journal of Hospital Administration 2010;26(6):432-434
The paper described the specific measures and results of postoperative complications reporting system, including reporting coverage, methods, deadline, data management, supervision and feedback. In addition, it introduced results of the practice and the positive impact on quality of care and safety management. Based on these, the authors proposed further improvements of the system for the purposes of constant improvement of care and higher medical safety.
6.A preliminary study on the toxin of earthworm and the methods to take it off
Zuxun ZHANG ; Liming HE ; Peilan YU ; Dianer YANG ; Hua LIU
Chinese Journal of Biochemical Pharmaceutics 2001;22(3):112-113
Purpose The source of earthworm toxin, its ingredients and the methods to take it off were studied.Methods The irritable secretor was dialyzed, and then examined by UV-Vis spectrum instrument.The proteins was analyzed with electrophoresis. The toxicity was tested in vivo.Results The proteins of irritable secretor 0.242mg/kg iv cause a mouse to death.Many bands were separated from the proteins with SDS-PAGE, among which two kinds were the principal. Even with different stimulus the secretor of earthworm was identical in ingredients.Conclusion The results demonstrated that the earthworm proteins of irritable secretion had toxicity and the toxin could be removed from earthworm by adding NaCl or 40V electricity.
7.Tuina massage for 120 cases of infants eczema.
Chinese Acupuncture & Moxibustion 2012;32(12):1103-1104
Child
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Child, Preschool
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Dermatitis, Atopic
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therapy
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Female
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Humans
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Infant
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Male
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Massage
9.~(99m)Tc-octreotide scintigraphy and planar X-ray mammography diagnosis of breast cancer
Xiaojiang HE ; Jinxiong HUANG ; Guibing CHEN ; Hao YU ; Hua WU
Chinese Journal of Medical Imaging Technology 2009;25(10):1892-1895
Objective To compare the value of (~99m) Tc-octreotide scintigraphy and planar X-ray mammography in the diagnosis of breast cancer. Methods A total of 32 patients with suspected breast cancer were enrolled into this study. All patients underwent (~99m) Tc-octreotide scintigraphy and planar X-ray mammography. The diagnostic efficiency of (~99m) Tc-octreotide scintigraphy and planar X-ray mammography were compared based on the post-surgery pathology. Results The sensitivity of (~99m) Tc-octreotide scintigraphy and planar X-ray mammography in the diagnosis of breast cancer was 91.67% and 66.67%, the specificity was 100% and 37.50%, the accuracy was 93.75% and 59.38%, respectively. Combining (~99m) Tc-octreotide scintigraphy with planar X-ray mammography, the sensitivity, specificity, accuracy, positive predicting values and negative predicting values for breast cancer was 95.83%, 100%, 96.88%, 82.14% and 47.06%, respectively. Conclusion The diagnostic efficiency of (~99m) Tc-octreotide scintigraphy for breast cancer is superior to planar X-ray mammography. (~99m) Tc-octreotide scintigraphy combined with planar X-ray mammography, the diagnostic efficiency for breast cancer will be further improved.