ObjectiveTo investigate the clinical value of routine para - aortic lymphadenectomy in patients with stage Ⅰ B1 and Ⅱ A2 cervical cancers treated by surgical intent.Methods240 patients with stage Ⅰ B1 and Ⅱ A2 squamous cervical cancer were randomly divided into tow groups( group A and B).120 patients (group A) underwent radical hysterectomy with systematic pelvic lymphadenectomy and paraaortic lymphadenectomy.Other 120 patients (group B) underwent radical hysterectomy with systematic pelvic lymphadenectomy,additional para-aortic lymphadenectomy were carried out when para-aortic lymph nodes were identified as suspicious by visualization and palpation.The operation time,amount of surgical bleeding,length of stay,complications,and metastasis rates of pelvic lymph node,metastasis rates of common iliac lymph node and metastasis rates of para-aortic lymph node were analyzed between two groups.ResultsThe clinical characteristics of the patients had no statistically significant difference between the two groups.The length of stay and the complications of group A were significantly higher than group B ( P ＜0.05 ).During the 240 patients,the metastasis rates of pelvic lymph node,common iliac lymph node and para-aortic lymph node were 27.9％,10.8％ and 5.4％.The metastasis rate of para-aortic lymph node in group A was statistically higher than that in group B( 8.3％ vs 2.5％,P ＜0.05),whereas there had no significant difference of the metastasis rates of pelvic lymph node and common iliac lymph node between the two groups.ConclusionsRoutine para-aortic lymphadenectomy can be helpful to accurately predict the status of the para-aortic lymph node in patients with stage Ⅰ B1 and Ⅱ A2 cervical cancer,but the length of stay and the complications of operation will increase.

Objective To explore the early diagnosis and treatment of delayed intraeranial haematoma.Methods To analyze the patients that were detected haematoma by first CT examine m Hospital, but were diagnosis of intracranial haematome consequently by CT reexamine and ICP monitor. Results 66 patients of delayed intracranial haematoma were evacuated and 30 patients died. The operative mortality was 45.5 %. Conclusion To burr bole in time and repeat CT scan will improve the prognosis of acute eneephalocele and deteriorating patients.

Influenza viruses are global epidemic and diversely difficult to distinguish,which threaten human’s survival and development very much.In recentyears,the frequent outbreaks of influenza prompt the rapid development of Influenza virus detection.Compare with the traditional isolated culture and immunological detection,molecular diagnostic technology is of high detection speed,high sensitivity and specificity,that gradually play an important role in the current Influenza virus de-tection.In order to provide a theoretical basis for the rapid diagnosis of Influenza virus in the clinic,the article summarize the update progress of molecular biology and diagnostic techniques of Influenza viruses.

Objective To study the clinical features, efficacy and complications of the radiotherapy for cervical cancers in aged women. Methods Basing on the clinical stages, histologic types, differentiation of neoplasms, macroscopic types, sizes of cervical masses and vagina,542 female patients ≥ 60 years of age with cervical cancers were analyzed. The survival rates and sequelae were observed. Results The clinical stages, histologic types and differentiation of tumors in both ≥ 60 and

15 0 mU/L treatment with 20 mg levothyroxine a day was needed. Conclusions The thyroid function of elderly patients, taking low oral dose of aminodarone for cardiac arrhythmias, especially those with cardiac pacemaker was affected early (on the 6th day) and significantly. However, it recovered spontaneously but gradually in most of the cases even with abnormal thyroid hormone levels throughout the study. Therefore, oral low dose amiodarone (in one year) is safe for the elderly patients, but they must be monitored closely in the first 6 months.

OBJECTIVETo compare clinical efficacy of individual osteotomy instrument and total knee arthrolplasty (TKA) in treating patients with knee osteoarthritis.

METHODSFrom June 2014 to December 2014, 40 patients with unilateral knee osteoarthritis were randomly divided into two groups and 20 cases were in each group. One group (individual group) were treated with TKA with individual osteotomy instrument,including 5 males and 15 females with an average age of (67.3 ± 6.5) years old; 8 cases on the left side and 12 cases on the right side. Another group (conventional group) were treated with conventional TKA , including 6 males and 14 females with an average age of (66.8 ± 7.3) years old; 9 cases on the left side and 11 cases on the right side. Operative time, blood loss, postoperative HSS score at 6 months, and changes of mechanical alignment before and after operation were analyzed.

RESULTSOperative time in individual group was (79.3 ± 4.7) min, and (83.5 ± 3.2) min in conventional group; blood loss in individual group was (287.1 ± 24.9) ml and (363.4 ± 47.2) ml in conventional group, there were statistical differences between two groups in these two items. There was no significant difference in postoperative HSS score at 6 months between individual group (84.8 ± 3.2) and conventional group (84.2 ± 2.5). Postoperative limb alignment in individual group was (2.8 ± 0.6)°, and (2.8 ± 0.6)° in conventional group, with no significant difference between two groups.

CONCLUSIONTKA with individual osteotomy instrument could reduce operative time and blood loss, but there was no differences in knee function, changes of mechanical alignment compared with TKA.

Adult ; Aged ; Arthroplasty, Replacement, Knee ; methods ; Case-Control Studies ; Female ; Humans ; Knee Joint ; surgery ; Male ; Operative Time ; Osteoarthritis, Knee ; surgery ; Osteotomy ; instrumentation ; methods ; Treatment Outcome

Chronic Disease ; prevention & control ; Humans ; Population Surveillance ; methods

Background and purpose:The cinobufacini injection is a traditional antitumor drug.However,its mechanism iS still unclear.The purpose of this study was to observe the effect of cinobufacini injections in DNA TOPO Ⅰ of human hepatocellular carcinoma HcpG-2 cells.Methods:The cells that were proliferated were assessed by MTT assay.Cell cycles were shown through FCM.TOPO Ⅰ mRNA expression was analyzed through RT-PCR.The activity of TOPO Ⅰ was measured by TOPO Ⅰ mediated super coiled PHR322 relaxation.Supercoiled PBR322 was also used to determine the direct DNA breakages.Results:Cinobufacini injections significantly inhibited HepG-2 cells proliferation in ways that were dependent on dosages and time.Induced tumor cells arrest at the S-phase.TOPO ⅠmRNA expression decreased in a manner that was dependent on dosages which inhibited the TOPO Ⅰ mediated DNA relaxations.However,the cinobufacini injections could not directly induce DNA breakage at any concentration.Conclusion:Cinobufacini injections can inhibit human hepatocellular carcinoma HepG-2 cells proliferation.The regulation of topoisomerase Ⅰ activity and mRNA expression may be one of the mechanisms that causes the cinobufacini injection to contribute against tumor.

Adult ; Arrhythmogenic Right Ventricular Dysplasia ; physiopathology ; Electrocardiography ; Humans ; Male ; Middle Aged

Objective: To establish the fingerprint of Althaeae Roseae Flos by HPLC and the molecular identification method of DNA barcode of rbcL sequence. Methods: The fingerprint establishment of Althaeae Roseae Flos was performed on Welchrom Column C18 (300 mm × 4.6 mm, 5 μm) with acetonitrile - 0.1% formic acid solution as mobile phase for gradient elution, with flow rate of 1.0 mL/min, column temperature of 35 ℃, detection wavelength of 365 nm, injection volume of 10 μL. DNA barcode molecular identification method was used for PCR amplification and determination of rbcL sequence. Results: The fingerprints of 11 samples were established, 21 common peaks were obtained, their similarities were calculated, and four components (hyperoside, quercetin, apigenin and kaempferide) were determined. The total length of rbcL sequence of 11 samples was measured, and the G + C content was 44.10%-44.40% and genetic distance (K2P) was 0.001 4. There were 10 ectopic points and the similarity was 99.00%. Conclusion: The two methods are stable and reliable, which can provide basis for the identification and quality control of A. rosea.