ObjectiveTo develop an experimental model of orthostatic hypotension (OH) after spinal cord injury (SCI) in rabbits and investigate the effect of tilting table exercise and Shengmaiyin on it. MethodsRabbit model of OH after SCI were induced by complete transection of spinal cord at the T5 spinal segment and raised to 60° head up tilt (HUT) on a tilting table. Tilting table exercise and Shengmaiyin were applied to different groups for 28 d from 4 days after operation. Systolic blood pressure (SBP) and pulse rate (PR) were observed with non-invasive sphygmobolometer of rabbit 3, 10, 17, 24, 31 d after the operation. Liquid chromatography with electrochemical detection(HPLC-ECD) was used to detect the concentrations of plasma norepinephrine (NE) and epinephrine(E) with horizontal position and HUT 60° on the 31st day. ResultsSBP with HUT 60° in rabbits of combined treatment group was significantly higher than that in rabbits of SCI group on the 24th day after operation,and the drop in SBP from horizontal position to HUT60° was significantly lower than that in rabbits of SCI group. SBP with HUT 60° in rabbits of exercise group and combined treatment group was significantly higher than that in rabbits of SCI group on the 31st day after operation. The drop in SBP from horizontal position to HUT 60° was significantly lower in rabbits of Shengmaiyin group, exercise group and combined treatment group than that in rabbits of SCI group on the 31st day after operation. The concentration of E and NE of SCI group, Shengmaiyin group, exercise group and combined treatment group was lower than that of sham group, but there was no significant difference among these groups. ConclusionThe treatment with tilting table exercise and Shengmaiyin can improve OH after SCI partially in rabbits without involving norepinephrine and epinephrine significantly. The period of treatment can be shorten when both therapies used together.

Objective To observe the effect of tilting table exercise and shengmaiyin on alpha 1 adrenogenic receptor (α1AR) in the superior mesenteric artery after spinal cord injury (SCI) in rabbits.Methods 46 rabbits were randomly divided into the sham group (n=6), SCI group (n=10), shenmaiyin group (n=10), exercise group (n=10) and combined treatment (shenmaiyin plus exercise) group (n=10). The rabbit model of SCI was established by complete transection of spinal cord at the T5 spinal segment. Tilting table exercise and shengmaiyin were applied to different groups for 28 days from 4 days after operation. The expression of α1aAR,α1bAR,α1dAR mRNA in the superior mesenteric artery was detected by RT-PCR at 31d after SCI.Results The mRNA expressions of α1aAR, α1bAR, α1dAR subtypes increased to different extent in rabbits of the shengmaiyin group, exercise group and combined treatment group compared with those of SCI group.Conclusion The role of tilting table exercise and shengmaiyin improving orthostatic hypotension after SCI in rabbits may be related to up-regulation of α1aAR, α1bAR, α1dAR mRNA in the superior mesenteric artery.

Breast-contour preservation(BCP)encompasses all strategies to preserve the contour of the breast following breast-conserving surgery (BCS) and immediate postmastectomy breast reconstruction (IBR). With the continuous advancement of breast surgery, postoperative outcomes and quality of life of breast cancer patients have been significantly improved. Previous studies reported on BCP as a new comprehensive parameter for evaluating outcomes of breast cancer treatment. Based on preoperative comprehensive evaluation of patients′ general condition, characteristic of breast tumor, and breast morphology, individualized surgical strategy is formulated to improve BCP for early-stage breast cancer and improve postoperative outcomes of patients. Combined with the morphological characteristics of Chinese women′s breasts, breast contour can be preserved not only by BCS and IBR, but also by nipple-areola complex-sparing mastectomy in some breast cancer patients, thereby reducing psychosomatic impact as a result of losing breast mound.

Background and purpose:This study aimed to explore the inlfuences on quality of life of patients with breast cancer in different stages undergone different styles of operations. Methods:During Apr. 2012 and Apr. 2013, a total number of 207 patients with breast cancer, including 61 patients undergone breast conserving surgery (BCS), 60 patients undergone immediate breast reconstruction (IBR) and 86 patients undergone modiifed radical mastectomy (MRM) reviewed or admitted in Beijing Tongren Hospital Afifliated to Capital Medical University were included in the research, in which FACT-B, SAS and SDS were used to assess the quality of life and negative emotions performing as the paired design. Results:The physical well-being, emotional well-being and the level of negative emotions such as anxiety and depression revealed signiifcant differences compared the patients who undergone BCS or IBR with those who undergone MRM in preoperative period (P<0.05), and there was no statistical difference in terms of other quality of life (P>0.05). At two and ifve years postoperatively, there was no signiifcant in the physical well-being, emotional well-being and negative emotions (P>0.05), whereas there was signiifcant differences in the other quality of life (P<0.05). Conclusion:BCS and IBR compared with MRM can obviously reduce the levels of preoperative negative emotions such as anxiety and depression, and improve the quality of life in long-term (two and ifve years postoperatively) to some degree.

The present article deals with the result of early repair of 105 cases of cleft palate within the first two years of life.The result is excellent without fistulae,ruptures or deaths.The early repair of the cleft palate has the advantages of small blood loss during operation,little postoperative reaction and better healing.The key points of surgical procedure and postoperative management are introduced in details.The safety of the early operation and its influence on the development of maxillofacial growth are also discussed.The deficiency of maxillofacial growth is related to surgical procedures of cleft lip and palate besides the congenital factors.Early repair of cleft palate enables the child to develop normal speech and is advantageous to psychological development of the child.

AIM: To study the antioxidative effect of three kinds of xanthone:methylswertianin(MET), bellidifodin(BEL) and decussatin (DEC) extracted from Swertia macrosperma C.B. clark. METHODS: Hydroxy radical was generated from H_2O_2 and VC-Fe~ 2+ system; MDA contents in liver homogenate and mitochondria were measured by thiobabituric acid assay; the hemolysis of RBC and the swelling extent of mitochondria were detected by spectrophotometric methods. RESULTS: Xanthones could inhibit the generation of MDA, the hemolysis of RBC, and the swelling of mitochondria. The inhibitory effect of BEL was stronger than that of MET and DEC. CONCLUSION: Xanthones possess a good antioxidant activity.

Objective:To study the influence of the serum cytokine levels of patients with Graves′ disease(GD) before and After Treated With ~ 131 I.Methods:Using the method of radioimmunoassay, the serum levels of interleukin-1(IL-1), interleukin-2(IL-2), interleukin-6(IL-6), interleukin-8(IL-8) and tumor necrosis factor(TNF-?) of GD 52 patients were measured before and after ~ 131 I, 30 age-and sex-matched healthy subjects served as controls, and the results were compared with that of the control group.Results:The levels of IL-1, IL-6, IL-8, TNF-? of GD patients were significantly higher than that of the control group(P

ObjectiveTo study infection status of voluntary blood donors and epidemic trends of related infectious diseases in Bengbu area.MethodsBlood infection markers(ALT, HBsAg,Anti-HCV,TP and Anti-HIV) of 130 118 voluntary blood donors were detected.Voluntary blood donors were divided into college students group,reservation group and street group.Then, the positive rates of blood infection markers of three groups were compared.ResultsAccounting for 4.41％ ,5 748 voluntary donors of 130 118 volunteers were unqualified.The positive infection rates of students group, reservation group and street group were 2.42％, 4.08％ and 4.85％.The statistical differences of ALT and anti-HCV among these groups were significant (x2 = 35.07,12.31,12.45,116.49, all P ＜0.01),and no statistical significance was found in ALT difference among these groups(x2 = 3.82,0.59, all P ＞0.05).ConclusionThe infection rates of these blood donation related diseases of voluntary donors in bengbu aera showed increasing tendency, and that of college students in bengbu area was relatively lower.

BACKGROUND: When the dynamic equilibrium between oxidation system and anti-oxidation system in the body is upset, the overly produced active oxygen-derived free radicals will attack the target organs, thus resulting in the damage to organs and big molecules, and then diseases. Total anti-oxidation capacity (T-AOC), superoxide dismutase (SOD), and nitric oxide synthase (NOS) activity are the majorindexes for the defense system of the body, and their status is closely related to diseases. OBJECTIVE:To explore the level of T-AOC, SOD and NOS in serum,and find out its relationship with lifestyle. DESIGN:Single sample investigation. SETTING:Department of Health Laboratory Technology, College of Public Health of Harbin Medical University. PARTICIPANTS:Totally 531 residents of Bin County aged 20 to 70years were recruited between September 2000 and May2001. MATERIALS:The reagents kits of T-AOC, SOD and NOS were manufactured by Nanjing Jiancheng Bio-engineering Institute. METHODS:The investigators surveyed the residents with the same questionnaire. Questionnaire was made and the investigators were trained in advance. The questionnaire was filled in item by item as required. Questionnaire items consisted of the general situation, financial status, dietary habit, hobby, status of health care, and mental factor. Finally 95 investigation indexes were completed. The activity of serum T-AOC, SOD and NOS of the 531 residents in Bin County was determined, respectively, with reagent kits produced by Nanjing Jiancheng Bio-engineering Institute. The lifestyle factors were analyzed by multivariate stepwise regression analysis,and informed consent was obtained from the participants. MAIN OUTCOME MEASURFS:① Serum T-AOC, SOD and NOS in the participants; ② lifestyle factors affecting T-AOC, SOD and NOS.RFSULTS:According to actual analysis, the 531 participants entered the results analysis. T-AOC was measured in 489 participants, SOD in 525participants, and NOS in 531 participants. ① Indexes of the participants:T-AOC of the males was obviously higher than that of the females [(20.01±7.21), (15.25±6.22) kU/L, P ＜ 0.05]. SOD of the males was slightly lower than that of the females while NOS of the males was slightly higher than that of the females, but there was no significant difference. ②Multivariate stepwise regression analysis showed that risk factors related to T-AOC were gender difference (OR=2.188), educational level (OR=1.859),and the presence of rheumatism, respectively (OR =1.142). SOD-related risk factors were educational level (OR=1.584), years of spirit drinking (OR =1.048), presence of nephritis (OR=1.093), and irradiation (OR=1.770);frequency of tea drinking was a beneficial factor (OR=0.800). NOS-related risk factors were the average amount of cigarette smoking (OR=1.194) and the times of weekly spirit drinking (OR=1.368). However, the beneficial factor that affected serum NOS was the frequency of weekly mutton eating (OR=0.458) CONCLUSION:This experiment revealed that the ability to clean free radical in the 531 subjects was good and that it was better in males than in females. The increased educational level and presence of rheumatism can decrease T-AOC. Drinking, smoking and irradiation decrease SOD activity while the frequency of tea drinking and mutton eating are beneficial factors.

Diabetic nephropathy presents an increasing trend worldwide. It has been an attractive area to find novel targets for the treatment of diabetic nephropathy. SIRT1 (Sirtuin 1), a member of deacetylation enzymes, regulates cell senescence, metabolism, and apoptosis. In last ten years, lots of studies showed that SIRT1 exerts a protective effect in the progression of the diabetic nephropathy by promoting reconstruction of energy homeostasis, modulating cell redox state, resisting cell apoptosis, inhibiting inflammation and ameliorating renal fibrosis. SIRT1 has become a potential new target for the treatment of diabetic nephropathy.
Apoptosis ; Cellular Senescence ; Diabetic Nephropathies ; pathology ; Humans ; Oxidation-Reduction ; Sirtuin 1 ; physiology