Objective To study the relationship between helicobocter pylori(Hp)infection and iron deficiency anemia(IDA)in children.Methods Children who had upper digestive symptom were had gastroscopy examination and Hp test.According to the examination results,all the patients were divi-ded into 2 groups,the observation group and the control group.All patients were detected blood routine,serum iron(SI),serum ftrritin(SF)and total iron-binding capacity(TIBC).Results The IDA morbidity was 52.27%(23/44 cases)in obsorvation group and 17.39%(8/46 cases)in control group,there was obvious difference between two groups(?2=12.12 P

Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. Results Psoralen from 1 to 20 μmol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration.

Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. ]Results Psoralen from 1 to 20 ?mol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration.

Objective:To study the better extraction method for Qidan decoction.Method:Orthogonal design was used to find befitting conditions.The water quantity,extraction time and frequency were selected as observational conditions.The optimum conditions were repeated three times to study repetition.Results:The optimum extracting procedure is 14 times water, extracting 3 times,the time was 30 min each time.The active ingredient content RSD of three parallel trial is 0.86%.Conclusion: The optimal condition for ultrasonic extraction is 14 times water,extracting 3 times,30 min per time.This method is of good repeatability.

Autosomal dominant polycystic kidney disease (ADPKD) is a most common inherited renal disease, about 50% with a family history, although the exact etiology not yet clear. To date, ADPKD, a multisystem disorder without effective preventive and therapeutic means, has been shown to be detrimental to human health. Recent studies show that severe oligoasthenozoospermia, necrospermia, immotile sperm, azoospermia, epididymal cyst, seminal vesicle cyst, and ejaculatory duct cyst found in male ADPKD patients may lead to male infertility, though the specific mechanisms remain unknown. Structural anomaly of spermatozoa, defect of polycystin, mutation of PKD genes, and micro-deletion of the AZF gene could be the reasons for the higher incidence of abnormal semen quality in male ADPKD patients. Assisted reproductive techniques can increase the chances of pregnancy, whereas the health of the offspring should be taken into consideration. This article presents an overview of reproductive issues concerning infertile male ADPKD patients from the perspective of the morbidity, pathophysiological mechanism, diagnosis, and management of the disease.
Cysts ; pathology ; Ejaculatory Ducts ; pathology ; Female ; Humans ; Infertility, Male ; physiopathology ; Kidney ; pathology ; Male ; Mutation ; Polycystic Kidney, Autosomal Dominant ; physiopathology ; Pregnancy ; Reproductive Techniques, Assisted ; Semen Analysis ; Spermatozoa ; pathology

AIM:To study the rationality and the best dosage of Qidan Decoction (Radix Astragali,Radix et Rhizoma Salviae miltiorrhizae,Fructus Psoraleae,etc.) on inhibiting gastric cancer metastasis. METHODS:The ability of inhibiting gastric cancer metastasis and the activity of NK cell were detected as indexes to appraise the rational and the best dosage. RESULTS:The function of Astragalus membranaceus,Salvia miltiorrhiza.Ege.,psoralea corylifolia and Lycium Barbarum on inhibiting gastric cancer metastasis and rising the activity of NK cell was significant(P

Adult ; Carcinoma, Hepatocellular ; secondary ; Humans ; Liver Neoplasms ; secondary ; Lymphoma, Non-Hodgkin ; pathology ; Male ; Spinal Neoplasms ; pathology

0.05). But typeⅢsodium channel expressed higher than that in control groups in hippocampus, restriction mapping analysis showed thatⅢN increased significantly (P

Objective To assess the effects of p27mt gene transfection on the proliferation and apoptosis of human hepatocellular carcinoma cell (HCC) lines SMMC-7721. Methods A replication deficient adenovirus vector encoding p27mt (Ad-p27mt) was used and p27mt cDNA was transfected into human SMMC-7721 cell lines in vitro. The synthesis of DNA in SMMC-7721 cells was determined by using 3H-thymidine incorporation; the cell apoptosis was determined by flow cytometry, TUNEL method and DNA fragmentation analysis. Results The virus titer was 7.95?1012 cfu/ml, the transduction efficiency was 100 % when multiplicity of infection ≥50, FCM analysis revealed a sub-G1 cell peak in Ad-p27mt transduced hepatocellular carcinoma cell lines. Agarose electrophoresis showed marked ladder .The difference of apoptotic index between the Ad-p27mt group and the control group was statistically significant (58.6?4.3, vs 4.5?1.6, P

Objective:To evaluate effects of different medication forms of dexmedetomidine on perioperative cognitive function in elderly patients undergoing radical operation of gastric carcinoma, and Ramsay sedation score.Methods:A total of 150 patients aged 65 years and 75 years scheduled for elective radical operation of gastric carcinoma from December 2018 to December 2019 in Shanxi Provincial Cancer Hospital were enrolled, and they were divided into 3 groups according to random number table method: different medication forms of dexmedetomidine groups (group A, group B) and the control group (group C), 50 cases in each group. The patients in group A and group B continued to pump dexmedetomidine at a rate of 0.5 μg·kg -1·h -1 and 0.3 μg·kg -1·h -1 respectively for 15 min before induction of anesthesia. And the patients in group C were given intravenous pumping of an equal volume of 0.9% NaCl for 15 min. Subsequently, patients in group A and group B received continuous intravenous infusion of 0.3 μg·kg -1·h -1 for 30 min before the end of operation, the patients in group C received 0.9% NaCl infusion of equal volume until the end of operation. The cognitive function of the patients was measured by using the Monterey cognitive assessment scale (MoCA) the day before surgery and on day 1,3 and 7 after surgery, and the incidence of perioperative neurocognitive disorder (PND) was counted. Ramsay sedation score at 30 min, 24 h and 48 h after surgery was compared among the three groups. Results:There were 3 patients in group A and 3 patients in group C were excluded because they were transferred to ICU due to serious postoperative complications. There were significant differences in MoCA score among the three groups on day 1, 3, 7 after operation (all P < 0.01); MOCA score of group A, B and C on day 7 after operation was (26.9±0.7) scores, (26.6±1.0) scores, (26.3±1.2) scores, respectively, and the difference between group A and group C was statistically significant ( P < 0.01). The incidence of PND among the three groups on day 1, 3, 7 after surgery had statistically significant differences (all P < 0.05), and the incidence of PND in group A was lower than that in group B and C (all P < 0.05). Ramsay sedation score among the three groups at 30 min and 24 h, 48 h after operation showed statistically significant differences (all P < 0.01), and that in group C was lower than that in group A and group B, and the differences were statistically significant (all P < 0.01). Ramsay sedation score at 24 h after operation in group A was high than that in group B and group C (all P < 0.01). Conclusions:Dexmedetomidine assisted with anesthesia can reduce the incidence of PND in elderly patients undergoing radical operation of gastric carcinoma and enhance the sedative effect. What's more, the most obvious effect is the infusion of 0.5 μg·kg -1·h -1 before anesthesia induction.