Objective To investigate the role of DRP1 and OPA1 in the process of apoptotic in hyperoxia induced lung injury of premature rats .Methods 48 premature Wistar rats were divided into control group in which rats were exposed tO 210 ml .L 1 oxy-en ,and hyperoxia group in which Rats were exposed to 950 mL .L 1 oxyen .The lung tissues of 2 groups were gained on the first day ,the second day and the seventh day with which that all mice were killed by cutting neck .Section of lungs were stained with hea-toxylin eosin to observe the pathological changes ,the protein expression in the lung cells which were linked with the change of plas-tosome ,then the premature rat lung tissue apoptosis in each group were evaluated .Results Compored with the contnx group ,the expression level of OPA1 in the hyperoxia group began to decrease on the first day and apparently decreased to the buttom line on the seventh day .On the other hand ,DRP1 in hyperoxia group began to increase on the first day ,while reached the summit on the seventh day .A few of TUNEL positive cells began to increase with time dependence .A lot of TUNEL positive cells could be found in hyperoxia group ,and the apoptotic index reached the peak on the 7 d .There is a significantly positive correlation between the cell apoptosis and the ratio change of DRP1 and OPA1 in high oxygen group(r=0 .725 ,P<0 .01) .Conclusion The classical pathologic characters of lung injury were found out in hyperoxia group ,and it changed obviously with time .

OBJECTIVE To strengthen the hospital supply Division standardized management and prevent the pathogenic microorganism dissemination effectively and safeguard the human health.METHODS Strengthening the vocational study,improving personnel′s knowledge level,formulating control system,strengthening detail and flow management and adopting the effective monitoring method all were the main measures.RESULTS The results were to guarantee the aseptic material quality,and prevent the occurreance of hospital Ainfection.CONCLUSIONS The scientific and effective management measures make our supply division a good quality management institution,with the programming the materiol processing and the standardizing the disinfection and antisepsis.

Biofilms ; Humans ; Sinusitis ; etiology ; microbiology

Adolescent ; Child ; Child, Preschool ; Endoscopy ; Esophageal and Gastric Varices ; surgery ; Female ; Humans ; Infant ; Ligation ; Male

Abortifacient Agents, Nonsteroidal ; pharmacology ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Female ; Pinellia ; chemistry ; Plant Proteins ; pharmacology ; Pregnancy

Objective To observe the clinical characteristics and efficacy of laser photocoagulation of aggressive posterior retinopathy of prematurity (AP-ROP).Methods Twenty-eight eyes of 14 infants with AP-ROP from May 2008 to December 2010 were enrolled in this study.The infants were examined using RetCam photography and indirect ophthalmoscopy.Among the 28 eyes,24 eyes were classified as zone one and 4 eyes zone two.All eyes were treated within 24 hours using binocular indirect ophthalmoscope and laser photocoagulation.The initial energy was 200 mW,using energy was 200 - 500 mW,exposure time was 200 ms.Every two photocoagulation spot was linked together,but no overlap.Follow-up ranged from 3 to 24 months,with a mean of 11.5 months. The retinal bloods,the iris surface vessels,the fiber hyperplasia on retina,retinal detachment or ruffle form were observed.Results Twenty-five of 28 eyes (89.3 ％) recovered or were classified as control; 1 of 28 eyes (3.6 ％) was suffered retinal detachment one month after treatment.The detachment was resolved through vitrectomy Surgery.Two of 28 eyes (7.1 ％)did poorly.The parents gave up treatment resulting in loss of vision.No treatment-related complications were observed during the follow-up period,such as damage to cornea,iris and lens. Conclusion Photocoagulation is a safe and effective way to treat most AP-ROP.

Celiac disease (CD) is a chronic autoimmune gastrointestinal disorder induced by ingestion of gluten-containing foods.In the past CD was thought prevalent only in Western countries,whereas it has been found common throughout the world and increasing numbers of Chinese patients has been reported recently.However,there are still many deficiencies in management of patients with CD in China and either the diagnosis or the treatment of CD is lacking evidence-based clinical pathway.We report a case of a young woman with CD presenting with chronic diarrhea,abdominal distention,and weight loss,whose outcome was improved by following evidence-based guidelines of medical nutrition therapy.Diagnosis and treatment of the patient was analyzed with review of foreign and domestic literature to provide readers reference of the standardized nutritional intervention of CD.

objective: To evaluate the clinical effect of various materials used in the reconstruction of mandibular defects retrospectively. Methods: 467 patients with mandibular defects were treated with various implantation materials during last 30 years and 395 of them were followed up.Results: 217 were satisfied with their mouth opening, occlusion and maxillofacial contour after sugery, 189 of them had bone transplanation and 28 had non biomateria transplantaion; 91 were partly satisfied, 72 of them had bone trasplantation and 19 had non biomateria; 87 were not satisfied, 11 of them had bone trasplantation and 76 had non biomaterial. Conclusion: Bone especially auto bone transplantation is more effective than non biomaterial in the reconstruction of mandibular deffects.

Objective To study the difference between central and peripheral position of contrast gray-scale imaging in rabbit′s liver.Methods Ultrasound contrast agent(UCA) SonoVue TM ( 0.1 ml/kg) was applied respectively in 8 normal rabbits by intravenous bolus injection.Corresponding parameters of the time-intensity curve: time to enhancement(ET),time to peak intensity(PIT),peak signal intensity(PSI),mean transit time(MTT) and area under the curve(AUC) were measured using low mechanical index contrast gray-scale imaging and Wash-in/Wash-out time intensity curve software.Results There was significant difference of parameters of the time-intensity curve between central position and peripheral position of parenchyma ( P

Objective To study the relationship between doses of ultrasound contrast agent(UCA) and contrast-enhancement effects of gray-scale imaging in rabbit liver. Methods Ultrasound contrast agent SonoVue TM in eight different doses ( 0.02 - 0.16 ml/kg) was applied respectively in five normal rabbits in randomized order as a bolus in a marginal ear vein followed by a 1.5 ml saline flush. Corresponding parameters of the time-intensity curve: time to enhancement(ET),time to peak intensity(PIT),peak signal intensity(PSI),mean transit time(MTT) and area under the curve(AUC) were measured using low mechanical index contrast gray-scale imaging and Wash-in/Wash-out time intensity curve software package. Results Along with an increase of the SonoVue TM dose,the MTT and the AUC increased exponentially,and the ET decreased exponentially. The PSI of portal vein and liver parenchyma increased only in the dose range from 0.02 ml/kg to 0.10 ml/kg ( r = 0.89 and 0.92 ,respectively),and decreased with a further increase of the UCA dose. A possible explanation for this effect is the bubble saturation and attenuation. No significant correlation was found between the UCA dose and the PIT( r = -0.35 and 0.22 ,respectively). Conclusions The parameters of time-intensity curve are influenced intensely by different UCA dose. It is important to grasp the relationship between the UCA doses and contrast-enhancement effects for microcirculatory quantification. The 0.10 ml/kg appears to be the optimal dose for tissue perfusion studying because it significantly improved videointensity and duration of enhancement and did not result in the bubble saturation and attenuation.