1.Effects of healthcare cooperative intervention on patients with psychiatric symptoms secondary to aortic dis-section
Jihong ZHONG ; Xuemin ZHU ; Dan WU ; Xia ZHANG ; Juan YU
Journal of Medical Postgraduates 2015;(11):1167-1170
Objective Aortic dissection is accute in occurrence along with fast change , which is inclined to to induce psy-chotic symptoms and affect the lives of patients .The study was to investigate the effects of healthcare cooperative intervention on pa-tients with psychiatric symptoms secondary to aortic dissection . Methods We established medical cooperation organization structure to clarify the division of responsibility along with a quick assessment form , a process chart and a green channel for emergencey treat-ment.The above method was applied to intervene 32 patients with psychiatric symptoms secondary to aortic dissection and observe the diagnosis time , the time from diagnosis to surgery , the remission time of psychiatric symptoms , the incidence of adverse events and the length of stay. Results 32 patients were diagnosed within 30min followed by emergency surgery within 30min after diagnosis, which was 1-2 hours shorter than the previous diagnosis and surgery preparation time .5-21 d after surgery , psychiatric symptoms have been effectively controlled.Extubation, falling out of bed, self-injury or injury and other adverse events did not occur .No patient died, and the average length of stay was 13.5d, which was 1.75d shorter than the length of stay of previous similar patients . Conclusion The healthcare cooperation method can quickly identify patients with psychiatric symptoms secondary to aortic dissection , prevent adverse events and shorten the length of stay .
2.Effects of combination of ketamine and N-acetylcysteine on brain damage following cerebral ischemia /reperfusion in mice
Xichong YU ; Xianyan CHEN ; Hongyu ZHOU ; Dan LIN ; Tongjun ZHU
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
AIM: To evaluate the effects of the combination of ketamine (KT) and N-acetylcysteine (NAC) on damage following cerebral ischemia/reperfusion in ICR mice. METHODS: Male ICR mice were randomly divided into seven groups: Sham group, NS (saline 0.1 ml?kg -1 ) group, KT (15 mg?kg -1 ) group, NAC (75 mg?kg -1 ) group, NAC+KT (75+15 mg?kg -1 ) group. (1) ICR mice underwent two hours cerebral ischemia by transient right middle cerebral artery occlusion (tMCAO) and followed 6 h and 24 h reperfusion. Then brains were prepared for the determination of the infarction volume. Before the death, neurological deficits were scored. (2) ICR mice subjected to five minutes ischemia by two common carotid arteries occlusion (2-VO) and followed 0.5 , 2 and 6 h reperfusion. Brains were prepared for the determination of the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of MDA. RESULTS: (1) tMCAO produced severe neurological deficits, decreased the average score and brought about large infarction volume. KT, NAC showed the improvement of the average score and reduced infarction volume to some extent, and KT+NAC improved significantly. (2) The content of the MDA, the activities of GSH-Px and SOD in 2-VO mice deteriorated sharply, KT, NAC reduced the content of the MDA, enhanced the activities of GSH-Px and SOD, NAC+KT significantly ameliorated the levels of MDA, increased the activity of SOD and GSH-Px. CONCLUSION: The damage of cerebral ischemia/reperfusion leads to the decrease of neurological score, the increase of infarction volume, the reduction of activities of SOD and GSH-Px and the elevation of MDA. KT and NAC partly relieve the damage, and NAC and KT in combination attenuates the damage more effectively.
3.Electromagnetic Compatibility of Medical Devices on Health-Care Train
Yu CHE ; Hanjun SUN ; Dan HUANG ; Changning SHI ; Lei ZHU
Chinese Medical Equipment Journal 2004;0(09):-
The problems of Electromagnetic Compatibility(EMC) and Electromagnetic Interference(EMI) of medical electric devices are directly related to the medical treatment effect for wounded patients on the health-care train.EMC and EMI problems of medical electric devices in use are studied and analyzed.Suggestions on preventing EMI problems are given to guarantee the normal running of medical electric devices in health-care train.
4.Role of platelet-derived growth factor after hemorrhagic shock in rats
Dan LAN ; Yu ZHU ; Xiaoyong PENG ; Liangming LIU ; Tao LI
Journal of Regional Anatomy and Operative Surgery 2015;(2):166-168,169
Objective To investigate platelet-derived growth factor ( PDGF ) protection on blood flow and mitochondrial function of hemorrhagic shock rats. Methods Ninety-six SD rats were randomly divided into six groups including shock group, lactated ringer's solution (LR) resuscitation group,PDGF treatment groups(1,3. 5,7,15μg/kg). Laster-Doppler and oxygen concentration determination method were applied to observe the protective effect of PDGF treatment on animal survival,blood flow and mitochondrial function in liver and kidney. Re-sults As compared with LR resuscitation group,PDGF treatment increased animal survival rate and also improved blood fiow of liver and kindy,mitochondrial respiration control ration(RCR),of which the group with 3. 5μg/kg had the best result. Conclusion This finding sug-gests that PDGF may be a potential agent to treat acute critical such as hemorrhagic shock.
5.Epidemiological surveillance of Henipavirus in Chongqing
Dan PENG ; Zhilei ZENG ; Dan ZHU ; Xiao CHEN ; Jianping YU ; Mingming XU ; Yingying ZHANG ; Qunling ZHAN ; Hong ZHAI ; Peng XIE
Chinese Journal of Microbiology and Immunology 2008;28(11):1050-1054
Objective To establish nucleic acid testing techniques for detecting Nipah virus (NiV) and Hendra virus (HeV), and to test the NiV and HeV in peripheral blood collected from domestic pigs, cows and goats in Chongqing. Methods Peripheral blood samples of 580 domestic pigs, 250 cows, 180 goats were collected from Chongqing since June 2007 to June 2008. The lymphocytes were separated by density gradient centrifugation and total RNA was extracted using Trizol method for detection of NiV and HeV with one-step real-time RT-PCR. Sequence identification and analysis were performed for positive PCR prod-ucts. Virus isolation and culture were adopted for positive samples, and epidemiologic reports were submit-ted. Results Nucleic acid detections searching for NiV and HeV were successfully performed in animal blood samples collected from Chongqing. "Takeoff points" were not found in fluorescence amplification curves of all samples. Curves kept the same slope, and assays were judged as negative. Conclusion Until now, Neither NiV or HeV infection has been found in domestic animals blood samples collected from Chongqing, which suggest a lower possibility of outbreaks of Nipah disease and Hendra disease in Chongqing in the near future.
6.Screening of Active Fractions from Huanglian Jiedu Decoction against Primary Neuron Injury after Oxygen-Glucose Deprivation.
Zhu-yan HUANG ; Bei-bei PAN ; Chun-yan HUANG ; Yi-lu YE ; Dan-dan LIU ; Yue-ping YU ; Qi ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(8):981-987
OBJECTIVETo observe the protective effect of active fractions of Huanglian Jiedu Decoction (HJD) on primary cortical neuron injury after oxygen-glucose deprivation (OGD)/reperfusion (R) injury. Methods Using macroporous resin method, HJDFE30, HJDFE50, HJDFE75, and HJDFE95 with 30%, 50%, 75%, and 95% alcohol were respectively prepared. Then the content of active components in different HJD fractions was determined with reverse phase high-performance liquid chromatography (RP-HPLC). The OGD/R injury model was induced by sodium dithionite on primary cortical neurons in neonate rats. MTT assay was used to observe the effect of four fractions (HJDFE30, HJDFE50, HJDFE75, and HJDFE95) and seven index components of HJD on the neuron viability.
RESULTSRP-HPLC showed active component(s) contained in HJDFE30 was geniposide; baicalin, palmatine, berberine, and wogonside contained in HJDFE50; baicalin, berberine, baicalein, and wogonin contained in HJDFE75. The neuron viability was decreased after OGD for 20 min and reperfusion for 1 h, (P <0. 01), and significantly increased after administered with HJD, HJDFE30, HJDFE50, and HJDFE75 (P <0. 05, P <0. 01). Geniposide, baicalin, baicalein, palmatine, wogonside, and wogonin could increase the cortical neuron viability (P <0. 05, P <0. 01).
CONCLUSIONSHJDFE30, HJDFE50, and HJDFE75, as active fractions of HJD, had protective effect on primary cortical neuron injury after OGD/R. Furthermore, geniposide, baicalin, and baicalein were main active components of HJD.
Animals ; Berberine ; Berberine Alkaloids ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Flavanones ; Flavonoids ; Glucose ; metabolism ; Iridoids ; Models, Animal ; Neurons ; Oxygen ; metabolism ; Rats ; Reperfusion Injury ; drug therapy
7.Advances in regulation of pregnane X receptor-mediated drug transporter
The Chinese Journal of Clinical Pharmacology 2018;34(20):2469-2472
Pregnane X receptor (PXR) is a ligand-activated transcrip-tion factor that is abundantly expressed in hepatocytes , its ligand drugs can regulate drug transporters expression and activity via PXR -mediated signaling pathways , and thereby affect the drug disposition ,increase the risk of drug -drug interactions and adverse effects when combined with clinical medication.This paper systematically reviewed the current re-search progress and clinical correlation of drug regulatory transporters me-diated by PXR.
8.Magnetic resonance tracking of endothelial progenitor cells labeled with superparamagnetic iron ox-ide homing to the site of hepatoma
Xiaoli MAI ; Haijian FAN ; Dan MU ; Decai YU ; Jun YANG ; Bin ZHU
Chinese Journal of Nuclear Medicine and Molecular Imaging 2016;(1):19-24
Objective To track the migration and incorporation of intravenously injected, magneti?cally labeled endothelial progenitor cells ( EPCs) from mouse bone marrow into the blood vessels in a rapid?ly growing HCC model by microMR (7.0 T). Methods This study was approved by the Institutional Com?mittee on Animal Research. H22 hepatic ascitic cancer cells was directly injected into the left liver lobe of BALB/c nude mice ( n=15) . EPCs derived from bone marrow of C57BL/6 mice were isolated and cultured. The third passage EPCs were collected and labeled with 25 μg/ml superparamagnetic iron oxide ( SPIO) and poly?l?lysine (PLL) complex (SPIO?PLL). MTT assay and flow cytometry were used to evaluate the difference of growth curve and apoptosis between labeled and unlabeled EPCs. EPCs labeled with SPIO?PLL were injected into mice via tail vein in experiment group (on the 3rd day after establishing HCC model) (n=15) and control group (n=6). The signal changes of tumor (the 1st, 3rd and 7th day after transplantation) were observed by microMR. Prussian blue staining and immunohistochemistry staining of CD31 were per?formed. MRI findings were confirmed by histomorphology. Two?sample t test was used to analyze the data. Results Single tumor was showed in the liver of all mice 3 d after establishing models. Labeling with SPIO?PLL at a concentration of 25μg/ml did not alter cell growth curve ( measured by MTT assay;t=0.281, P>0.05) and cell apoptosis (analyzed by flow cytometry). The apoptosis rates of SPIO?PLL labeled and un?labled EPCs were (12.31±1.43)% and (11.57±1.24)% in early stage, and (0.55±0.07)% and (0.49± 0?05)% in late stage. No significant differences were observed between them (t=0.967, 1.060; both P>0?05) . Migration and incorporation of transplanted and labeled cells into tumor were documented with in vivo microMR as low signal intensity at the tumor periphery as early as the 3rd day after EPCs administration in preformed tumors (4/5). Prussian blue staining showed iron?positive cells at the sites corresponding to low signal intensity on MRI. The positive cells expressing CD31 existed in intratumoral and peritumoral vessels. There was no signal change in control group at all time points. Conclusions MRI can demonstrate the in?corporation of magnetic labeled mouse EPCs into the implanted hepatoma. It may be helpful for early diagno?sis and therapy of liver tumor.
9.Study of cognitive and affective theory of mind in adolescents with schizophrenia
Dandan LI ; Chunyan ZHU ; Xiaosi LI ; Kai WANG ; Hui ZHONG ; Fengqiong YU ; Dan LI
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(7):598-600
Objective To explore the theory of mind (TOM) between adolescent with schizophrenia and normal adolescent from hot TOM and cool TOM.Methods 37 adolescent schizophrenias and 25 normal adolescents were interviewed with the Chinese version of YONI task, Reading the Mind in the eyes test and series of tests,then analyzed with adolescent schizophrenias cognitive features.Results ① Compared with the control group (83.16± 10.07),the scores of YONI task in the adolescents with schizophrenia (74.30 ± 11.44) had statistically significant difference (t=-2.983,P<0.05),mainly the statistically significant difference existed in the groups in cool and hot TOM.②The differences between positive subtype (69.85± 12.66) and negative subtype (78.18±8.92) in scores were significant(t=-2.104,P<0.05),especially in the hot TOM.③In Reading the Mind in the eyes test,there was no statistically significant difference in scores(t=0.982,P>0.05) between the control group (17.59±3.13) and the group of adolescents with schizophrenia(18.42±2.98).Conclusion The adolescents with schizophrenia damage in hot TOM and cool TOM.The adolescents with schizophrenia in positive subtype are worse than the negative subtype,especially in the hot TOM.
10.The assessment and comprehensive nursing interference of pain after thoracic surgery
Jihong ZHONG ; Rong HU ; Xuemin ZHU ; Xia ZHANG ; Yulian SUN ; Dan WU ; Juan YU ; Xin ZHOU
Journal of Medical Postgraduates 2015;(2):189-191
Objective Because of the major trauma from thoracotomy , postoperative pain may arouse patients'psychological and physiological stress response and hence affected the treatment outcome and functional recovery seriously .We retrospectively studied the correlation between the staging of pain and nursing interference to investigate the effect of nursing interference on the pain intensity after thoracic surgery . Methods Five hundred and eighty cases of patients surviving thoracotomy between December 2013 and March 2014 in Nanjing Jingling Hospital were reviewed .Correlations between comfortable nursing measures such as effective analgesia , postural care , catheter care , environmental interventions , psychological intervention and the standard assessment of pain were analysed according to postoperative pain stage . Results With comprehensive nursing interference , the highest pain score occurred in the first 24 hour (2.89 ±0.39).The score was reduced gradually to 2.25 ±0.90 in stage Ⅱ, 1.58 ±0.57 in stage Ⅲ and 1.06 ±0.24 in stage Ⅳrespectively . Conclusion Comprehensive nursing interference according to pain staging may relieve pain effectively after thoracotomy .