1.Left ventricular functional changes of stunned myocardium during perioperation of coronary artery bypass graft surgery
Liang-liang, ZHAO ; Hang, L(U) ; Hong-yu, LIU ; Bai-chun, WANG ; Guo-wei, ZHANG ; Li-guo, YANG ; Chao, CHI
Chinese Journal of Endemiology 2012;31(3):283-286
ObjectiveTo evaluate the functional changes of stunned myocardium before and after coronary artery bypass graft(CABG) treatment,and clear the meaning of revascularization which CABG has brought to patients with diffused vascular changes.MethodsA total of 36 patients with 99% diffused coronary artery stenosis in left anterior descending branch underwent non-pump CABG treatment in the Department of Cardiothoracic Surgery the First affiliated hospital of Harbin Medical University.Real-time three-dimensional echocardiography (RT3DE) was repeatedly performed 1 week before operation and 10 days,1 month,and 3 months after CABG.Regional diastolic volumes,systolic volumes,ejection fractions,regional stroke volume to global diastolic volume and the values of abnormal segments before and after CABG were studied.ResultsOne week before operation and 10 days,1 month and 3 months after CABG,the differences of volumes between groups in the last phases of diastole and systolic were statistically significant in anterior wall basement segment,anterior septal basement segment,anterior wall intercalary segment,anterior septal intercalary segment,anterior wall of apex cordis and septation of apex cordis(F =3.51,3.55,4.08,4.05,2.98,3.01,all P < 0.05; F =4.51,4.55,4.08,3.00,2.96,2.99,all P < 0.05).The values of the six segments mentioned above,3 months after operation[(6.74 ± 1.23),(6.64 ± 1.21),(6.02 ± 1.10),(5.95 ± 1.09),(5.82 ± 1.06),(5.10 ± 0.93)ml; (2.74 ± 0.50),(2.69 ± 0.49),(2.51 ± 0.46),(2.32 ± 0.42),(2.36 ± 0.43),(2.03 ± 0.37)ml] were compared with those of 1 week before operation[(8.33 ± 1.52),(8.20 ± 1.50),(7.43 ± 1.36),(7.36 ± 1.34),(7.19 ± 1.31),(6.29 ± 1.15)ml; (4.94 ± 0.90),(4.85 ± 0.88),(4.53 ± 0.83),(4.18 ± 0.76),(4.25 ± 0.78 ),(3.65 ± 0.67)ml],the differences were statistically significant (all P < 0.05); the differences between groups in regional ejection fractions,regional-global ejection fractions were statistically significant(F =4.56,4.88,4.28,3.15,2.93,2.88,P < 0.01 or < 0.05; F =5.56,5.28,4.98,5.15,3.03,2.78,P < 0.01 or < 0.05).Compared with 1 week before the operation, 1 month after the operation in regional ejection fractions,10 days,1 month in regionalglobal ejection fractions after the operation,4 segments of them were significantly improved(all P < 0.05) and 3 months after operation,all the 6 segments had been improved significantly(all P < 0.05).The maximum volume of the sum of group difference of the 6 segments and the 4 segments in the last phase of diastole was statistically significant(F =2.58,5.81,P < 0.05 or < 0.01 ),and the summation began to decrease 10 days after the operation.The values of 3 months after operation[ (36.27 ± 1.10),(25.35 ± 1.16)ml] were compared with that of 1 week before operation[ (44.80 ± 1.36),(31.32 ± 1.43)ml ] the difference was statistically significant (all P< 0.05).The maximum volume summafion comparisons of 6 segments and 4 segments in the last phase of systolic had statistical significance(F =5.77,5.57,all P < 0.01 ),and 10 days after the operation,the summation began to decrease.The values of 1 month[(16.4 0 ± 0.48),(11.58 ±0.51 )ml],and 3 months after operation[ (14.65 ± 0.45),(10.26 ± 0.46)ml],were compared with those of 1 week before operation[ (26.40 ± 0.80),(18.50 ± 0.84)ml],the differences were statistically significant (all P < 0.05).ConclusionsStunned myocardium can be improved through CABG in myocardium systolic,diastole function and ejection fractions of the relevant segments and all of this have proved that patients undergoing CABG revascularization can improve the heart function of the ischemic area.
2.Determination and evaluation of arsenic speciation and glutathione level in lever and blood of mice subchronically exposed to inorganic arsenic
Yu-hong, MU ; Chun-qing, QU ; Yuan, ZHONG ; Xiao-yun, YU ; Ge-xin, LI ; Xiu-qiang, L(U) ; Gui-fan, SUN ; Ya-ping, JIN
Chinese Journal of Endemiology 2008;27(3):260-263
Objective To explore the distribution of arsenic speciafion and to estimate the effect of arsenic on glutathione(GSH)levels in the blood and liver of mice exposed to different concentrations of inorganic AsⅢ through drinking water.Methods Mice drank water containing arsenite at concentrations of iAsⅢ of 0(contr01),25,50,100 ms/L for 6 weeks.Blood and liver were sampled to asses$the levels of inorganic arsenic(iAs),monomethylarsenic acid(MMA),dimethylarsenic acid(DMA)by the method of hydride generation trapping and ultra-hypothermia coupled with atomic absorption spectrometry,and the level of GSH by the method of 5,5'-Dithio-bis (2-Nitrobenzoic acid).Results Leveh of iAs.MMA and DMA in blood and in liver increased along with the increase of iAs concentrations in drinking water.Primary methylated index(PMI)and secondary methylation index (SMI)of liver and blood were significantly higher in exposed groups than those in control group(P<0.05).SMI of liver in 50 mg/L exposed group[(50.45±2.94)%]was significantly higher than those in 25 mg/L and 100 mg/Lgroups[(41.68±7.09)%and(41.19±8.87)%,respectively],the difference being statistically significant(P<0.05).The ratio of iAs.MMA and DMA in blood and liver in exposed group were 2:3:5 and 4:3:3,the percentage of level of organic arsenic(MMA+DMA)were 80%and 60%.GSH in blood and liver in exposed group decreased along with iAs concentrations in drinking water and had significant differences compared with those in control group (P<0.05).However,levels of GSH in liver and blood did not differ significantly between exposed groups and control group(P>0.05).Conclusions Membolism of iAs in liver is maximized when the iAs concentrations in drinking water increases to a certain level.However,the percentage of arsenic speciation in blood is different from that in liver,suggesting that other organs and tissues may be capable of methylation of inorganic arsenic.The level of GSH in liver and blood in mice is a good mark tO reflect the toxicity of arsenic.
3.Clinical effects of carboprost methylate suppositorites in general term vaginal delivery patients with postpartum hemorrhage
Jing-Ying HUANG ; Xuan CHEN ; Yu-Chun L(U)
The Chinese Journal of Clinical Pharmacology 2017;33(6):502-505
Objective To analysis the effect of misopmstol,oxytocin nasal spray and carboprost methylate suppositorites on estrogen,nitric oxide (NO),nitric oxide synthase (NOS) and clinical efficacy in patients with general term vaginal delivery.Methods A total of 600 patients with postpartum hemorrhage were randomly divided into group A,group B and group C,200 cases in each group.Group A was given oxytocin nasal spray,per nostril 0.1 mL,twice a day,group B was given misoprostol,200 μg sublingual,group C received carboprost methylate suppositorites 1 mg,anus.After treatment,the serum levels of estradiol (E2),nitric oxide (NO) and nitric oxide synthase (NOS),bleeding volume,clinical efficacy,adverse drug reactions were compared in three groups.Results After treatment,the total effective rates in group A,B and C were 76.50% (153/200 cases),86.00% (172/200 cases),90.50% (181/200 cases),total effective rate of group C significantly higher than group A (P < 0.05).After treatment,estradiol levels of group A,B,C were (14.75 ±2.09),(17.63 ±2.15),(21.82 ±3.09) nmol · L-1.Levels of NO in group A,B,C were (107.83 ± 11.21),(92.42 ± 10.17),(79.25 ±9.89) nmol · L-1.Levels of NOS in group A,B,C were (25.64 ±3.11),(22.56±3.05),(19.63 ±2.28) nmol · L-1(P <0.05).And 2 h bleeding volume were (712.43 ± 40.85),(632.43 ± 42.77),(580.74 ± 37.64) mL in group A,B,C;2-24 h bleeding volume were (138.92 ± 17.86),(126.94 ± 12.93),(103.85 ± 11.13) mL in group A,B,C(P <0.05).The adverse drug reactions in group A were nasal irritation,nasal bleeding,incidence of adverse drug reactions was 1.50% (3/200 cases).The adverse drug reactions in group B were mild nausea,vomiting,dizziness,incidence of adverse drug reactions was 2.50% (5/200 cases).The adverse drug reactions in group C were diarrhea,nausea and vomiting,incidence of adverse drug reactions was 2.00% (4/200 cases),with no statistical differences (P > 0.05).Conclusions Compared with the treatment of misoprostol and oxytocin nasal spray,the carboprost methylate suppositorites can significantly improve the serum E2 level,reduce serum NO and NOS levels in patients with postpartum hemorrhage and reduce bleeding.
4.Effect of cytochrome P450 2C19 gene polymorphism on clopidogrel antiplatelet therapy
Hui MA ; Hui-Yi L(U) ; Xiao-Jie YU ; Zeng-Chun HU ; Li-Jing SUN ; Li-Chun CHENG ; Ce ZHANG
The Chinese Journal of Clinical Pharmacology 2017;33(19):1909-1911
Objective To investigate the effect of cytochrome P450 2C19 (CYP2C19) gene polymorphism on the antiplatelet therapy of clopidogrel in patients who underwent percutaneous coronary intervention (PCI).Methods Fifty-five patients who underwent PCI,as well as twenty-three healthy subjects,were enrolled in this study.The genotypes of the samples were identified by gene chips hybridization.The contents of soluble P-selectin (CD62p) and platelet glycoprotein GP Ⅱ b/Ⅲ a fibrinogen receptor(PAC-1) in patients who underwent PCI were detected by flow cytometry (FCM).The expressions of platelet activation markers were calculated according to interquartile range method.Results Seven patients with CYP2C19 * 2/* 2 genotypes were poor metabolizers,while twenty-eight patients with CYP2C19 * 1/* 2,CYP2C19 * 1/ * 3 were intermediate metabolizers,twenty patients with CYP2C19 * 1/ * 1 were extensive metabolizers.In intermediate metabolism (* 1/* 2),intermediate metabolism (* 1/* 3) and poor metabolism group,the CD62p expressions were (6.73 ± 5.69) %,(10.94 ± 9.80) %,(14.35 ± 6.24) %,(16.80 ± 13.65) %,respectively,statistically significant differences were found when compared with the control group;The PAC-1 expressions were (1.06 ± 0.69) %,(2.10 ± 4.09) %,(2.37 ± 3.15) %,(2.89 ± 2.75) %,and there were significant differences in CD62p and PAC-1 among the groups(P <0.05).The genotypes of extensive metabolism was high in the response to clopidogrel,while those of intermediate metabolism were moderate and poor metabolism were low.Conclusion Intermediate metabolism genotype patients could be adjusted to the dose of clopidogrel or the use of other antiplatelet drugs,and the poor metabolism genotype patients should be used other antiplatelet drugs.
5.Twenty six cases of human Fasciola gigantica infection in Dali, Yunnan province
Mu-xin, CHEN ; Lin, AI ; Xue-nian, XU ; Shan, L(U) ; Jian-ming, JIAO ; Hui-yong, SU ; Wei, ZANG ; Ting-jun, ZHU ; Yu-chun, CAI ; Jia-jun, LUO ; Jia-xu, CHEN
Chinese Journal of Endemiology 2012;31(6):595-598
Objective The aim of the study was to investigate the epidemic situation,clinical symptom,diagnosis and epidemiological characteristics of human Fasciola gigantica infection in Dali,Yunnan province.It will also provide a scientific basis for fasciolosis control and prevention.Methods Epidemic data were collected and patient's clinical signs and symptoms were studied.Serum soluble antigen of Fasciola gigantica of patients and part of family members and health people in the same village was detected using enzyme-linked immunosorbent assay (ELISA) and the eggs of Fasciola gigantica in stool were observed under microscope.Sequencing and PCR amplification of Fasciola gigantica eggs had been done.Sequencing results were analyzed using basic local alignment search tool (BLAST) program of the U.S.National Center for Biotechnology Information (NCBI) and the similarity of the two in the sequence of nucleic acid was compared.Furthermore,patients were experimentally given orally therapeutic doses of Triclabendazole 10 mg·kg-1·d-1 daily for 2 days,and kept in the hospital for observation for one week.Moreover,host and vector were investigated in the surrounding ditches of Dali prefecture and Limnaea peregra snail samples were collected.All the snails were squashed by glass sheet in order to detect the cercarie.Cow dung and sheep manure was collected in the Limnaea peregra distribution environment,and the eggs in the feces were checked by microscope after washing and precipitation.Results All the 26 patients had a continued hyperpyrexia with distinct alimentary system symptoms of nausea,vomiting,stomachache,abdominal distension as well as hepatomegaly,sensitive to percussion,different levels of liver damage detected by CT.All the patients had an eaten history of raw Herba Houttuyniae and other aquatic plants,and the course of the disease was similar,with the same epidemiological characteristics.ELISA detection was used in the 26 patients,family members and other healthy population,the results of all the 26 patients were positive(100.0%,26/26) ; the positive rates of the 57 family members and other health people of the same village were 31.6% (18/57) and 17.1% (6/35),respectively.The results of sequencing and BLAST program showed that the pathogen was Fasciola gigantica with the similarity between 99%-100%.PCR amplification also confirmed that the eggs were Fasciola gigantica eggs with an approximately 1000 bp band on agarose gel.After treatment with Triclabendazole,body temperature of the patients dropped to normal and symptoms improved markedly.Moreover,329 Limnaea peregra snails were collected including 5 ones with redia and one-tailed cercariae which were preliminary identified as the larva of Fasciola gigantica.There were also eggs of Fasciola gigantica detected in one stool of cattle and one of goat.Conclusions Eating raw food is the leading cause of the onset of the disease.Triclabendazole is the drug of choice to treat Fasciolasis.Health education should be strengthened by government and disease prevention and control departments in order to make the local residents to understand the potential hazard of eating raw aquatic vegetable and drinking unboiled water,which is the key to prevent the occurrence of the disease.
6.A computer aided design approach of all-ceramics abutment for maxilla central incisor
Yu-Chun SUN ; Yi-Jiao ZHAO ; Yong WANG ; Jing-Yun HAN ; Ye LIN ; Pei-Jun L(U)
Chinese Journal of Stomatology 2010;45(10):631-634
Objective To establish the computer aided design (CAD) software platform of individualized abutment for the maxilla central incisor. MethodsThree-dimentional data of the incisor was collected by scanning and geometric transformation. Data mainly included the occlusal part of the healing abutment, the location carinae of the bedpiece, the occlusal 1/3 part of the artificial gingiva's inner surface,and so on. The all-ceramic crown designed in advanced was "virtual cutback" to get the original data of the abutment's supragingival part. The abutment's in-gum part was designed to simulate the individual natural tooth root. The functions such as "data offset", "bi-rail sweep surface" and "loft surface" were used in the process of CAD. ResultsThe CAD route of the individualized all-ceramic abutment was set up. The functions and application methods were decided and the complete CAD process was realized. Conclusions The software platform was basically set up according to the requests of the dental clinic.
7.17-16,17-Dihydroxycyclooctatinyl-hexaketide ester from Streptomyces sp. SR107.
Meng-Yu-Jie LIU ; Shan-Ren LI ; L U CHUN-HUA
Chinese Journal of Natural Medicines (English Ed.) 2016;14(12):931-933
A new hexaketide acid esterified by the 17-hydroxyl group of 16,17-dihydroxycyclooctatin, namely 17-[16,17-dihydroxycyclooctatinyl]-hexaketide ester (1), a member of the group of rare bacterial diterpenes with a fused 5-8-5 ring system was isolated from strain Streptomyces sp. SR107. The structure was determined on the basis of its spectral data (H NMR, C NMR, H-H COSY, HSQC, HMBC, NOESY, IR and HR-ESI-MS). The antibacterial activity was also evaluated in this paper.
Anti-Bacterial Agents
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chemistry
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pharmacology
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Bacteria
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drug effects
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Diterpenes
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chemistry
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metabolism
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pharmacology
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Esters
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chemistry
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pharmacology
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Streptomyces
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chemistry
8.Predictive value of admission amino-terminal pro-B-type natriuretic peptide on in-hospital mortality in patients with decompensated heart failure
Bing-Qi WEI ; Yue-Jin YANG ; Jian ZHANG ; Ke-Fei DOU ; Yu-Hui ZHANG ; Xiao-Hong HUANG ; Lian-Ming KANG ; Chun-Ling ZHANG ; Qing GU ; Xin GAO ; Yan-Min YANG ; Yan DAI ; Li-Tian YU ; Hui-Min ZHANG ; Rong L(U)
Chinese Journal of Cardiology 2009;37(6):481-485
Objective To evaluate the predictive value of admission plasma amino-terminal pro-B type natriuretic peptide(NT-proBNP)on in-hospital mortality in patients with decompensated heart failure.Methods Plasma NT-proBNP levels were measured in patients with decompensated heart failure within 24 hours after admission with ELISA method.The NT-proBNP levels were compared between survivals and dying patients in hospital.ROC analyses were performed to evaluate the predictive value of admission plasma NT-proBNP on in-hospital mortality and to identify the optimal NT-proBNP cut-point for predicting in hospital mortality.A binary logistic regress analyses was used to evaluate if NT-proBNP was an independent predictor for in-hospital mortality.Results A total of 804 patients with decompensated heart failure were enrolled in his study(293 valvular heart diseases,219 ischemic cardiomyopathy,141 dilated cardiomyopathy,14 hypertrophic cardiomyopathy,21 restrictive cardiomyopathy,39 hypertensive heart disease,41 chronic pulmonary heart disease and 36 adult congenital heart disease)and 96 patients were in class Ⅱ,450 in classⅢand 258 in cases Ⅳ according to NYHA Classification.During hospitalization,64 deaths were recorded and the on admission plasma NT-proBNP levels of patients died during hospitalization were significantly higher than those of survivals[4321.1(3063.8,6606.5)pmol/L VS.1921.6(873.9,3739.2)pmol/L,P<0.01].Area under receiver operating characteristic curve(AUC)of NT-proBNP to predict in-hospital death was 0.772(95%CI:0.718-0.825,P<0.01),the optimal plasma NT-proBNP cut-point for predicting in-hospital mortality Was 3500 pmol/L,with a sensitivity of 70.3%,a specificity of 72.0%,an accuracy of 71.9%.a positive predictive value of 17.8%and a negative predictive value of 96.6%.Patients whose NT-proBNP levels were equal or more than 3500 pmol/L had a much higher in hospital mortality(17.8%)compared with those with NT-proBNP levels of less than 3500 pmol/L (3.4%),P<0.01.Binary logistic regress analyses demonstrated that admission plasma NT-proBNP,pneumonia,heart rate and NYHA class were independent predictors for in-hospital mortality in patients with decompensated heart failure(P<0.05 or 0.01)and admission plasma NT-proBNP Was the strongest predictor for in-hospital mortality.Conclusions Admission plasma NT-proBNP level was an independent predictor for in-hospital mortality in patients with decompensated heart failure.The optimal NT-proBNP cut point for predicting in-hospital mortality was 3500 pmol/L in this patient cohort.
9.Initial evolution research for design and process accuracy of one type of domestic computer aided design soft and computer aided manufacture
Yang SONG ; Yi-Jiao ZHAO ; Yu-Chun SUN ; Pei-Jun L(U) ; Yong WANG
Chinese Journal of Stomatology 2013;48(9):550-553
Objective To evaluate the design and manufacture accuracy of a domestic computer aided design(CAD) and computer aided manufacture(CAM) system,and to compare it with similar foreign products.Methods Thirty models of posterior-teeth-single-crown preparations were collected,and STL data of these preparations was collected by Denmark 3Shape scanner.Three copings were made for each preparation,the one designed and manufactured using commercial CAD/CAM system (3Shape CAD software and Wieland T1 CAM equipment) was assigned into control group T0,the one designed and manufactured using domestic CAD software (developed by Peking University School and Hospital of Stomatology and Nanjing University of Aeronautics and Astronautics) and Wieland T1 CAM equipment was assigned into experimental group TCAD for design accuracy evaluation,and the one designed and manufactured using 3Shape CAD software and domestic CAM equipment(developed by Peking University School and Hospital of Stomatology,Tsinghua University and ShanDong XinHua Incorporated Company of medical apparatus and instruments) was assigned into experimental group TCAM for manufacture accuracy evaluation.Finally,the marginal fitness were compared and evaluated by using 3D & Profile measurement microscope laser.Results The marginal fitness of TCAD was 27.98 (19.10,46.57) μm in buccal,32.67 (20.65,50.82) μm in lingual,27.38 (22.53,52.61) μm in mesial,29.50 (22.68,53.65) μm in distal; of TCAM was 21.69(15.87,30.21) μm in buceal,18.51(13.50,22.51) μm in lingual,19.15(15.42,26.89) μm in mesial,22.77(18.58,32.15) μm in distal; and there were no statistical differences compared with T0 [20.16(17.16,48.00) μm in buccal,21.51(17.05,28.31) μm in lingual,23.54(17.89,30.04) μm in mesial and 23.94(17.93,28.19) μm in distal] except lingual data of TCAD.Conclusions The design and machining precision of this domestic CAD/CAM system is at the same level of those comparable foreign products.