1.Effects of Early Enriched Environment on Expressed of Growth Associated Protein 43 in Hippocampus of White Matter Damaged Neonatal Rats
zheng-yu, ZHANG ; bao-li, YUAN ; su-juan, DENG
Journal of Applied Clinical Pediatrics 2006;0(13):-
Objective To study the effects of early enriched environment on expression of growth associated protein 43(GAP-43) in hippocampus of white matter damaged(WMD) neonatal rats.Methods Two days old postnatal SD rats (n=29) were collected and randomly divided into normal control group(n=9),intervention group(n=10) and non-intervention group(n=10).The latter 2 groups were subjected to WMD at first.The right common carotid artery was dissected and ligated,and after 2 hours rest,exposed to 60 mL?L-1 oxygen 940 mL?L-1 nitrogen gas mixture.After that,intervention group received the neonatal handling and was kept in an enriched environment for 27 days.Neonatal handling was applied from the 4th day to the 10th day after WMD.Then intervention group was put into enriched environment from the 11th day to the 30th day after WMD.The other 2 groups were fed in normal environment.The abilities of sensorimotor function (hanging test and inclined plane test) were observed after intervention,and Western blot was used to examine the expression of GAP-43 in hippocampus at the 7th day and the 27th day after intervention.Results The sensorimotor function abilities of intervention group were much higher than those in non-intervention group (P
2.MRI and Pathological Features of Ovarian Endometrioid Adenocarcinoma
Xueping ZHENG ; Mingping MA ; Shun YU ; Leizhi KU ; Qiang BAO
Chinese Journal of Medical Imaging 2015;(9):697-700,706
PurposeTo study the MRI and pathological features of ovarian endometrioid adenocarcinoma (OEC) in order to evaluate the value of MRI in diagnosing OEC. Materials and Methods The MRI imaging features of 8 patients confirmed by surgery and pathology were analyzed retrospectively and were compared with the results of surgery and pathology. The MRI outcome and the related histological findings were further analyzed.Results Six out of the eight patients had unilateral tumor, 3 tumors in the left and the other 3 in the right; 2 patients had masses in the both ovaries. The total number of masses was ten.The diameters of the tumors ranged from 3.5 to 16.5 cm, with the average size of (10.5±4.1) cm. The border of 2 tumors was partially fuzzy and that of the other 6 was clear. The MRI scans showed that 8 tumors were cystic-solid and the other 2 were solid with heterogeneous signals. The solid components mainly presented slightly short T1 signals and long T2 signals; the cystic ones revealed long T1 and T2 signals. The enhanced scanning showed that the solid components of 8 tumors were patchy and obviously enhanced and the other 2 had mild or moderate enhancement. The cystic components were not hyper-intense. The enhanced MRI scans of two cases of primary endometrial carcinoma with metastases to the ovaries showed thickened endometrium and mild hyper-intense.Conclusion MRI can reveal the pathological features of OEC and clearly presents the forms, components and the relationship with its surroundings of tumors. Therefore, MRI is of great importance to the clinical diagnosis of OEC.
3.Controlled clinical studies on drug-induced liver injury in the elderly Chinese patients: a literature review
Ying ZHANG ; Gansheng ZHANG ; Zhijun BAO ; Songbai ZHENG ; Xiaofeng YU
Chinese Journal of General Practitioners 2013;12(11):890-893
Objective To review the clinical features of drug-induced liver injury (DILI) in elderly Chinese patients.Methods A literature search was conducted with the key wordsdrug-induced liver injury ordrug-induced liver disease andelderly; four Chinese medical databases were used:including Chinese Biomedical Literature Database (CBM),Chinese Science and Technology Periodical Database (VIP),Wanfang Database and China National Knowledge Infrastructure (CNKI),from when the bases established to December 2011.Data from retrieved literatures of retrospective controlled studies of DILI were analyzed by SPSS 14.0 software.Results Five studies met the inclusion criteria,including 275 elderly patients (aged group) and 602 non-elderly patients (non-aged group) with DILL There was no significant difference in gender between the two groups (P > 0.05).Clinical manifestations of jaundice,fatigue,anorexia,and skin itching were more commonly seen in the aged group (P < 0.01).Cholestatic hepatic injury occurred more frequently in the aged group than in the non-aged group [24.9% (61/245) vs.18.8% (102/245),P < 0.05],while hepatocellular type occurred more frequently in the non-aged group [64.3% (350/544) vs.55.1% (135/245),P < 0.05].More DILI were induced by Chinese herb medicine and cardiovascular drugs in the aged group than in the non-aged group [47.0% (79/168) vs.35.9% (93/254) and 8.3% (14/168) vs 1.2% (3/259) respectively,P < 0.05].As for the prognosis,there was no significant difference in clinical cure and improvement rate between the two groups [84.4% (141/167) vs.81.5% (291/357),P >0.05].Conclusion The elderly are more sensitive to the drugs and have a high risk of DILI.Prevention and timely diagnosis and treatment of DILI are particularly important for elderly patients.
5.Signet ring cell carcinoma arising from mature cystic teratoma of the ovary.
Hong-fang ZHENG ; Bao-yu JIANG ; Dan-hua SHEN
Chinese Journal of Pathology 2005;34(9):610-611
Adult
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Antineoplastic Agents
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therapeutic use
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Carcinoembryonic Antigen
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metabolism
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Carcinoma, Signet Ring Cell
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drug therapy
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metabolism
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pathology
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surgery
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Cell Transformation, Neoplastic
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pathology
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Cisplatin
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therapeutic use
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Female
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Humans
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Hysterectomy
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Keratin-20
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metabolism
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Ovarian Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
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Teratoma
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drug therapy
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metabolism
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pathology
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surgery
6.Entery of calcium into ECV304 endothelial cell via CaCa~(2+) activated non-selective cation channel
Dejie YU ; Guanghong BAO ; Lin LIN ; Yongfang ZHENG ;
Chinese Pharmacological Bulletin 2003;0(07):-
AIM To investigate the pathways of Ca 2+ entry into ECV304 endothelial cell and the effect of angiotensin Ⅱ(AⅡ) on calcium activated non setective cation channel(CAN). METHODS The cell attachment and whole cell configurations of patch clamp technique were used to record channel activity. RESULTS (1) The single channel conductance is ? o=(12 90?2 11) pS( n =4) for Ca 2+ passing through CAN of ECV304 cell in condition of pipette solution without K + and Na + but composed 120 mmol?L -1 CaCl 2. The channel current amplitude and open time can be enhanced by 1?10 -7 mol?L -1 AⅡ. The enhanced conductance in CAN is ? 1=(22 18?2 29) pS( n =4). The results of whole cell recording are identified with single channel recording. (2) The whole cell configuration was carried out for recording voltage dependent Ca 2+ channel in ECV304 cell. The peak current amplitude was (29 32?3 56) pA( n =4). This current was inhibited to (6 00?3 94) pA( n =4) by nifedipine and activated by BayK8644. CONCLUSIONS (1)Ca 2+ enters ECV304 cell via Ca 2+ activated non selective cation channel and voltage dependent L type calcium channel. (2) AⅡ can significantly enhance the calcium entry via CAN in ECV304 cell.
7.Overexpression of Alcohol DehydrogenaseⅠ in Saccharomyces cerevisiae
Li-Na QIN ; Xian-Zhang JIANG ; Bao-Yu TIAN ; Zheng-Yu SHU ; Jian-Zhong HUANG ;
Microbiology 1992;0(02):-
To improve ethanol production in Saccharomyces cerevisiae,an integration plasmid pUPGKAT with PGK promoter(phosphoglycerate kinase promoter),adh1 gene(the coding sequences of alcohol dehydrogenaseⅠ) and CYC1 terminator(Cytochrome c transcription terminator) was constructed.Firstly,a fusion fragment composed of PGK promoter and adh1 gene was generated by over lap extension PCR and ligated into pUG6 resulting in plasmid pUPGKA.Subsequently,CYC1 termi nator was amplified from pSH65 by PCR and ligated to the SpeⅠand SacⅡrestriction site of pUPGKA.To integrate PGK-adh1-CYC1 into S.cerevisiae genome,pUPGKAT was digested by TthⅢⅠand the lin-earized plasmid was used to transform S.cerevisiae YS2-△adh2(adh2 disrupted strain) by lithium acetate method.The yeast mutant YS2-△adh2-adh1 which had the adh1 gene placed under the PGK promoter and harbored the adh2 deletion was constructed.Anaerobic fermentation showed overexpression of adh1 by PGK promoter resulted in a 8.84% higher ethanol production compared to YS2-△adh2.
8.Cloning and Expression of Acyl Carrier Protein Gene from Schizochytrium
Zhi-Ping LI ; Xian-Zhang JIANG ; Bao-Yu TIAN ; Zheng-Yu SHU ; Jian-Zhong HUANG ;
Microbiology 1992;0(02):-
Acyl carrier protein is an essential component involved in the biosynthesis of DHA(Docosahexaenoic Acid) via PKS(Polyketide synthase) pathway,which takes the growing acyl chain from one enzyme to another.One cDNA clone,with high homology of ACP,was isolated from Schizochytrium sp.FJU-512 cDNA library.The deduced amino acid sequence contained 142 residues with isoelectric point of 5.04 and had the 4'-phosphopantetheine prosthetic(4'-PP) binding site.The target fragment was digested with BamHⅠ/HindⅢand inserted into the expression vector pET-30a resulting in the plasmid pET-30a/acp.The recombinant vector was transformed into E.coli BL21(DE3) and induced by IPTG.SDS-PAGE analysis demonstrated that ACP was effectively expressed.
9.Zeus robot-assisted laparoscopic cholecystectomy in comparison with manual laparoscopic cholecystectomy
Hanxin ZHOU ; Yuehua GUO ; Xiaofang YU ; Shiyun BAO ; Jialin LIU ; Yue ZHANG ; Yonggong REN ; Qun ZHENG
Chinese Journal of General Surgery 1997;0(06):-
Objective To evaluate Zeus robot-assisted laparoscopic cholecystectomy. Methods Forty patients were divided into two groups receiving respectively Zeus robot-assisted laparoscopic cholecystectomy (group A, 20 cases), and laparoscopic cholecystectomy (group B, 20 cases). A variety of clinical parameters were evaluated. Compared between these two groups. Results Camera clearing (1.1?1.0) times and time used for operation field adjustment in group A (2.2?0.7) min were significantly less than those in group B (4.5?1.5,7.5?1.2) min. Dissection actions(337?86)times and operative errors(10%) in group A were less than those in group B(389?94) times,25%. The operation time(104.9?20.5) min and setup time (29.5?9.8) min in group A were longer than those in group B (78.6?17.1) min,(12.6?2.5) min. The blood loss,and postoperative hospital stay were similar. There were no postoperative complications in either groups, and conversion to open surgery was done in one each patients. Conclusions Compared with laparoscopic technique, Zeus robotic surgical system offers greater ability of controlling operation field, precise and stable operative manipulations though it requires longer operation time.
10.Logistic regression discriminant analysis of relative factors for choledocholithiasis
Hengyu TIAN ; Hanxin ZHOU ; Shiyun BAO ; Jinfeng ZHENG ; Zhuo ZHANG ; Xiaofang YU
Chinese Journal of General Surgery 1994;0(05):-
Objective To investigate a detective methods of choledocholithiasis.Methods Using retrospective study methods, a logistic regression descriminant analysis of 16 factors related to choledocholithiasis was made and a relative discriminant model was constructed.Results Logistic regression analyses that had sex, history of jaundice or jaundice, the widest inner diameter of choledochus, AST,ALT and the history of cholecystectomy, cholecystolithiasis and pancreatitis included into discriminant model, gave the best predictive result. A test sample showed the discriminant model had a sensitivity of 89.4%, and a specificity of 80.0%.Conclusions Discriminant analysis of logistic regression with clinical data is helpful for diagnosis and treatment of choledocholithiasis.It can also increase the accuracy of the predicion of cholecystolithiasis and serve as a clinical guide.