Objectives:To investigate the prevalence situation and antibiotic resistance of chlamydia trachomatis (CT),ureaplasma urealyticum(UU) and mycoplasma hominis(MH) in infertile female patient,and to analyze the resistant of mycoplasma to common antibiotics. Methods:The cervical secretion samples from 2186 female infertility and 210 pregnancies women were collected. Then chlamydia trachomatis antigen was detected by immunoassay. Mycoplasma (UU and MH) were isolated and tested antimicrobial susceptibility for 12 kinds of antimicrobial drugs. Results:In the trial group 173 were CT-positive at a rate of 7.9%,and 1102 were positive for the mycoplasmae at a rate of 50.4%,of which 987 were UU infections and 115 were MH infections.The number of CT,UU and MH infections totaled 1275 cases,leading to an overall infection rate of 58.3%. The top three antibiotics for drug sensitivity in the UU,MH and UU+MH cultures were josamycin,minocycline and clarithromycin.The three antibiotics,to which the pathogens were most tolerant,included ofloxacin,norofloxacin and sparfloxacin. Conclusion:The infection rate of chlamydia and mycoplasma in infertility women is obviously higher than normal pregnanted,this shows the fact that chlamydia and mycoplasma infection of genitourinary tract may be one reason of infertility.The sensitivity of mycoplasma to common antibiotics especially to quinolones and macrolides has decreased.

Chronic lung disease is a very common complication caused by inhaled hyperoxia, mechanical ventilation and pulmonary infection in preterm infants. It shows early inflammation and late alveoli fusion with mesenchymal fibroblast proliferation. Mitogen activated protein kinase is a very important signal transduction pathway in eukaryotic cells.It plays an important role in the cell inflammation, proliferation, differentiation, survival and apoptosis, which may contributes to the chronic lung disease.

Objective:To search the effects and possible mechanisms of Lianggesan on the acute lung injury(ALI) rat induced by endotoxin. Methods: Endotoxemia was induced by intravenous lipopolysaccharide (LPS,5 mg/kg), then treated with Lianggesan. The levels of TNF-?, IL-1?and IL-10 in serum and wet-to-dry weight ratio of lung were detected. Results:In LPS group the levels of TNF-? began to increase at 1h, which reached the peaks at 2h(P

Objective To evaluate the vascular changes of uterus and ovary in women with recurrent spontaneous abortions(RSA).Methods Fifty women were enrolled into this study: women with RSA(RSA group,30 cases) and normal women with sterility(control group,20 cases).In the mid-luteal phase of a non-pregnancy cycle,transvaginal pulsed Doppler ultrasonography of the uterine artery and ovarian artery were performed.Resistence index(RI),pulsatility index(PI),endometrial thickness,serum estradiol, progesterone were determined.Results ①The endometrial thickness in RSA group was less than that in the control gruop[((8.3)?(1.7))mm and((9.4)?(1.2))mm,respectively,P

Rational use of antibiotics can delay the emergence of antimicrobial resistance, and antimicrobial resistance surveillance provides the basis for standardizing clinical rational use of antibiotics. Therefore, antimicrobial resistance surveillance network is of great importance, and its construction can improve the rational use of antibiotics.The construction of antimicrobial resistance surveillance network should emphasize the capacity building of clinical microbiology laboratory and enhance quality control of resistance data based on the standards put forth by Clinical and Laboratory Standards Institute ( CLSI) .

OBJECTIVE: To summarize the cause and mechanic of inflammation reaction induced by medical biomaterials in the body.DATA SOURCE: A computer-based online search of Pubmed database was undertaken to identify the relevant articles published from January 2004 to December 2008 in English with the key words of "biomaterials, inflammation, mechanism".Meanwhile, with Chinese articles were retrieved from CNKI database between January 2005 and December 2008 with the key words of "biomaterials, inflammation, mechanism".DATA SELECTION: Articles concerning the cause and mechanic of inflammation reaction induced by medical biomaterials were included. Articles addressing physical and chemical features and mechanics of medical biomaterials were excluded.MAIN OUTCOME MEASREUS:①After the material implants in vivo, inflammatory cell and material adhering.②Cell factor expression.RESULTS: After initially examined 70 literatures were obtained. According to inclusion and exclusion criteria, the reasons and the mechanism related to the medical biological material in host in vivo for causing the inflammation were analyzed. Along with the tissue engineering development, various new high-polymer medicine biological materials with good biological compatibility,the biological activity and the biodegradation absorption function are emerging unceasingly. The biomedicine material was used to repair or substitute damage organization and the organ, and to cause function recovery. The infection was still the serious complication following the medical biological material was implanted. The mechanism of biomedicine material-induced infection is: complement in blood adhere the biological material surface, which causes collection and adherence of integrin-mediated leukocytes, release of cytokines and growth factors from adherent monocytes/macrophages, resulting in the occurrence of biomedicine material-related infection. To prevent the medical biological material-related inflammation, we should first embark from the material itself, and seek for some to respond the small medical material to the host inflammation.CONCLUSION: The success of material implantation depends on the medical biological material relevant inflammation. The production of biomaterial-related inflammation is action results of inflammatory cell, inflammation factor, complement as well as enzyme, oxygen free radical. Material surface microstructure, chemistry and dielectric and so on immediately influence inflammatory cells on material response.

The International Society on Thrombosis and Haemostasis has proposed to hold World Thrombosis Day on October 13 annually from 2014 to improve awareness and education of thrombosis. Thrombotic diseases are multidisciplinary disorders involving multiple systems.Thus, multidisciplinary team treatment for thrombotic diseases should be developed in China.A comprehensive study of genetic factors for thrombotic diseases is expected to achieve early diagnosis and risk prediction for this type of disease, and thus precision diagnosis and individualized treatment can be achieved.

Objective To explore the application value of color Doppler ultrasound in screening of fetal con-genital heart disease and its significance.Methods 4 132 cases of the expectant mothers were selected,using color Doppler ultrasonography on the line screening of five section to detect fetal cardiac,to compare with the actual clinical situation and analyze the occurrence of fetal congenital heart disease.Results Among 50 cases(1.21%) of congeni-tal heart disease,28 cases were live births, accounted for 56.00%,13 cases of induced abortion, accounted for 26.00%,9 cases died,accounted for 18.00%.48 cases were detected by ultrasound,relevance ratio was 1.16%,the accuracy rate was 96.00%,the missed diagnosis rate was 4.00%.28 cases of live births undergoing surgical correc-tion,treatment effect was good,48 cases were detected,including 10 cases of ventricular septal defect,6 cases of tetral-ogy of Fallot,6 cases of atrial septal defect,5 cases of endocardial cushion defect,4 cases of single chamber,4 cases of persistent truncus arteriosus,3 cases right ventricular dysplasia,3 cases of tricuspid valve shift malformation,2 cases of persistent left superior vena cava,2 cases left ventricular dysplasia,2 cases of aortic arch interruption,1 case of rhabdomyoma.Conclusion Using color Doppler ultrasound detection for pregnant women can effectively detect severe congenital heart disease in the fetus,stop gravid timely,which can effectively improve the level of prenatal and postnatal care that is worth clinical application.

Objective: To explore the application and clinical effects of humanistic service management in the management of NICU.Method: Humanistic service,management and education are conducted among neonate patients,their family members,nursing staff,and nursing students in NICU,and the outcome clinical effects are investigated.Result: The application of humanistic service dramatically increases the positivity,activeness,creativity,and cohesiveness of nursing staff,improves their overall quality,helps to establish a harmonious relationship between nurses,nursing students,and neonate patients' family members,enhances the satisfaction degree of neonate patients' family members and the nursing standard in NICU,and promotes the general improvement of hospital nursing service.Conclusion: The application of humanistic service in NICU initiates nursing service,improves hospital nursing standard,promotes the general development of NICU nursing,thus deserves to be further extended and applied.

Severe burn is often accompanied by multiple organ damage. Acute lung injury (ALI) is one of the most common complications, and often occurs in the early stage of severe burns. If it is not treated in time, it will progress to acute respiratory distress syndrome (ARDS), which will be a serious threat to the lives of patients. At present, the treatment of ALI in patients with severe burn is still remained in some common ways, such as the liquid resuscitation, the primary wound treatment, ventilation support, and anti-infection. In recently, human umbilical cord mesenchymal stem cells (hUCMSCs) have been found having some good effects on ALI caused by various causes, but few reports on the efficacy of ALI caused by severe burns were reported. By reviewing the mechanism of stem cell therapy for ALI, therapeutic potential of hUCMSCs in the treatment of severe burns with ALI and a new approach for clinical treatment was provided.