Objective To investigate the clinical effect of enzyme linked immunosorbent assay (ELISA) in detecting hepatitis C antibody. Methods 200 cases of patients with hepatitis C was measured by ELISA, the gold standard method for detection of hepatitis C virus antibody, recording the test results, the data input SPSS software to give the corresponding analysis and conclusions. Results 200 cases of patients with hepatitis C were successfully completed the gold standard method and ELISA method for detection of hepatitis C virus antibody, the analysis showed that positive rate of ELISA method for detection of hepatitis C virus antibody was significantly higher than that of the gold standard method, the positive rate of ELISA was 82.50％, the positive rate of the gold standard method for was 50.00％, results suggested that HCV detection rate of ELISA method is significantly better (P<0.05). Conclusion The method of ELISA can be used for the detection of hepatitis C antibody, which has positive significance in improving the diagnostic accuracy of hepatitis C, and is beneficial to protect the efficacy and quality of life of patients.

Female ; Heart Defects, Congenital ; surgery ; Heart Septal Defects, Ventricular ; surgery ; Humans ; Male ; Pulmonary Artery ; abnormalities ; Pulmonary Atresia ; surgery

Humans ; Occupational Exposure ; prevention & control ; Respiratory Protective Devices ; classification ; standards ; utilization ; United States

Objective To summarize the ruptured types and surgical treatment experience for rupture of aortic sinus aneurysm. Methods Nineteen cases with aneurysm of aortic sinus were retrospectively analyzed. There were 10 cases with ventricular septal defect,6 cases with aortic valvular incompetence,6cases with other diseases,2 cases complicated by infective endocarditis. All cases underwent operational treatment. Results There were 15 cases of right aneurysm of aortic sinus,including 12 cases among them ruptured into right ventricle,3 cases rupture was penetrated into right atrium.There were 3 cases of null aneurysm of aortic sinus,including 1 case among them ruptured into right ventricle,2 cases ruptured into right atrium. There was 1 case of left aneurysm of aortic sinus, which ruptured into left ventricle. No operative death occurred in all, 1 case with aortic regurgitation light to moderate,and all cases were recovered.Conclusions The right aneurysm of aortic sinus is the most common types,and always rupture into right ventricle. It is important that aneurysm of aortic sinus should be operated as early as possible in order to avoid losing the chance of operation, particular in the patients complicated by infective endocarditis.

Adult ; Aged ; Antineoplastic Agents ; therapeutic use ; Benzamides ; Chemotherapy, Adjuvant ; Female ; Follow-Up Studies ; Gastrointestinal Neoplasms ; drug therapy ; surgery ; Gastrointestinal Stromal Tumors ; drug therapy ; secondary ; surgery ; Humans ; Imatinib Mesylate ; Liver Neoplasms ; secondary ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Piperazines ; therapeutic use ; Pyrimidines ; therapeutic use

Biomarkers ; analysis ; Genomics ; methods ; trends ; Humans ; Preventive Medicine ; methods ; trends

OBJECTIVE:To investigate the profitability of listed pharmaceutical enterprises in China,and to investigate the ba-sic profitability path. METHODS:With the help of DuPont financial analysis system,the financial data of the listed companies and other manufacturing industry during 2007-2013 were collected and compared in terms of the return on equity(ROE),profit margin (PM),asset turnover (ATO) and equity multiplier (EM),etc. RESULTS & CONCLUSIONS:During 2007-2013,average ROE and PM of listed pharmaceutical companies both were higher than those of other manufacturing industry(10.00% vs. 6.40%, 11.50% vs. 6.49%);while average ATO and EM were lower than other manufacturing industry (0.65 vs. 0.73,1.90 vs. 2.19), with statistical significance(P<0.05). It has advantages in core earnings and product profitability while disadvantage in asset turn-over and financial leverage for the listed pharmaceutical companies. Listed pharmaceutical companies’gross margin was higher (42.87% vs. 22.07%),but was offset by the higher ratio of expenses to sales(18.24% vs. 5.61%)and insufficient utilization ratio of circulating assets. To change this situation,listed pharmaceutical enterprises should focus on circulation cost saving and the im-provement of assets operation ability,especially enhancing the utilization ratio of current assets.

Objective To explore the effects of ulinastatin(UTI) on renal apoptosis and expression of bcl-2 in rats with severe acute pancreatitis(SAP).Methods Sixty rats weighing 250-300 g were randomized divided into 3 groups:pseudo-operation group(SO group,n=20),SAP group(n=20) and UTI treated group(UTI group,n=20).The model of SAP was established by retrograde injection of 5% sodium taurocholate solution into the biliopancreatic duct in the rats.Serum Cr and BUN were determined.The left kidneys were resected for light and electronic microscopic study.Renal cell apoptosis was determined by TUNEL.Expression of bcl-2 was detected by immunohistochemical staining of SABC.Results Serum Cr,BUN,renal cell apoptotic index and bcl-2 expression were markedly increased in SAP group compared with SO group(P

Returning astronauts had experienced decreased immune function and increased vulnerability to infection during spaceflights.In immune system,cell adhesion molecules(CAMs) play an important role in regulating immune response in normal physiological conditions.Studying changes of CAMs under microgravity could not only understand the effects of microgravity and its molecular mechanism on immune function,but also help to study the relative mechanism about cell sensation of microgravity.In this review,we will introduce some downstream signal pathways,gene expression and the effects on cell functions under microgravity.All of them are regulated by cell adhesion molecules related with the immune system.

Gravity is the most fundamental biomechanical stimulus for posture change.Pressure of blood flow is one of primary indicators to evaluate cardiovascular performance.Up to now, the underlying mechanism of effects of posture change on cardiovascular system is still unclear.A 3D FSI mathematical model with posture change was presented.By applying the body force terms to the fluid equation and the vessel wall equation, the model could be used to study posture change and the effects of gravity on the pressure of blood flow quantitively.Under different inlet-outlet pressure difference(IODP) and different postures such as horizontal, upright and upside-down one, the effects of gravity were simulated.In horizontal position, the pressure distributions of blood flow transformed from 2D(two-dimensional) axis-symmetry without gravity to 3D asymmetry with gravity under small IODP.With IODP increasing, gravity had less effects on pressure distribution and extreme value.As IODP reached 10 665.6 Pa(80 mmHg) and 2 666.4 Pa(20 mmHg) respectively, this effect was observed to be constant.Similar results were obtained from 3D fluid-only model.In either upright or upside-down position, 2D axis-symmetric pressure distribution was observed with and without gravity, yet the position, in which extreme pressure appeared, was different in upright position from that in upside-down one.Finally, the effect intensity of gravity in upright or upside-down position was more than twice as much as that in horizontal one.The results indicate that introducing body force term into the fluid and solid equations to present a novel model, which was based on hemodynamics, will provide a new way to study posture change.Effects of gravity on pressure distribution and extreme value changed with different postures and IODP.If IODP is small, ignoring effect of gravity and postures so as to simplify the hemodynamics model to 2D axis-symmetric one, the conclusion should be drawn with caution.