Humans ; Occupational Diseases ; diagnosis

OBJECTIVE: To discuss the biosafety and biocompatibility of biomaterials. METHODS: A computer-based online search of CNKI, CBM and VIP as well as manual search were performed to collect articles about random cell control experiments and animal experiments of biological material biocompatibility published between 1990 and 2008. A total of 28 Chinese literatures were collected, and 7 were included mainly involving the cytotoxicity test methods and blood compatibility of the experimental medium, experimental grouping, experimental materials, methods of observation, experimental results, and experimental conclusion. In addition, biocompatibility of the biological material was analyzed to summarize the biocompatibility of the biological safety. RESULTS: Based on experiments of biosafety and biocompatibility of biomaterials, the cytotoxicity test and blood compatibility of various biomaterials, such as collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, are essential for the biological safety. The experimental results have shown that the biomaterials have good biocompatibility. CONCLUSION: A variety of biological materials present good biocompatibility, including collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, based on the evaluation criteria.

Objective To evaluate the value of DSA image mosaic in arteriography in lower extremities. Methods 22 suspected cases were examined by bolus chase, and then the images were mosaiced to a full picture after processing by Paste to judge the image effects of vessels. Results This method could display the whole image by one injection of contrast media. Vary lesions were found in the 22 cases. Conclusion This method can display the lower extremities lesions directly, which is better than traditional DSA.

Objective To compare the safety and efficiency of non -ionic monomer diagnostic opaque, iodixanol (Visipaque) and that of non -ionic disomer diagnostic opaque, iohexol (Omnipaque). Methods By comparing retrospectively the DSA opacification of 60 patients from 2006 to 2007, patients′ indispositions, adverse reactions and pictorial efficiency were recorded respectively. Results In the control group, iodixanol showed less indisposition caused by injection than iohexol and there′ s no obvious difference of indisposition. Both images met diagnostic requirements. Conclusion Both iodixanol and iohexol are safe, efficient and well -tolerant diagnostic opaque. Iodixanol is better than iohexol in indisposition caused by injection.

The development of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR McAbs) marked a significant milestone in metastatic colorectal cancer (mCRC) treatment. The addition of anti-EGFR McAb can greatly improve quality of life of mCRC patients and mCRC prognosis and markedly increases the overall survival rate from 6 months to nearly 30 months. KRAS and NRAS mutations contribute to the primary resistance to anti-EGFR therapy and can serve as well-established predictive markers for pa-tient selection. Apart from the RAS family, other molecular alteration in EGFR signaling pathway may also compromise the efficacy of anti-EGFR treatment. In addition, patients who responded to anti-EGFR treatment eventually develop acquired drug resistance within 13 and 18 months. In this review, the mechanisms underlying the primary and secondary resistance to anti-EGFR therapy are summa-rized, and a possible strategy to circumvent drug resistance is proposed. We hope this review can provide compelling evidence, deeper insights, and reasonable guidance to facilitate the precise molecular targeted therapy of mCRC.

Objective To explore the value on application of combined C-reactive protein(CRP), serum albumin(PA)with blood routine inspection in diagnosis of bacterial infectious diseases in children.Methods 98 cases of patients confirmed infectious diseases in our hospital were selected as the study objects, and 68 cases with bacterial infectious diseases were as the bacterial infection group, while 30 cases with non-bacterial infection were as the non-bacterial infection group.Another 50 cases of healthy children were as the control group.All the children and children in the control group were given CRP, PA and blood routine detection, The diagnostic coincidence rates were evakuated according to the examination Results .ResultsCRP and WBC levels in the bacterial infection group were significantly higher than those in the non-bacterial infection group and the control group (P<0.05), PA level in the bacterial infection group were significantly higher than that in the non-bacterial infection group and the control group (P<0.05), The children's urine and cerebrospinal fluid or sputum bacterial culture results as the gold standard, 68 cases were true positive, and 30 were negative.CRP diagnosis showed 40 cases were positive, 58 cases negative, 13 cases false positive, and 41 cases false negative, the detection sensitivity was 39.71%, specificity was 56.67%, and coincidence rate was 44.90%.PA diagnosis showed 43 cases were positive, 55 cases negative, 12 cases false positive, and 37 cases false negative, the detection sensitivity was 45.59%, specificity was 60.00%, and coincidence rate was 50.00%.WBC diagnosis showed 47 cases were positive, 51 cases negative, 14 cases false positive, and 35 cases false negative, the detection sensitivity was 48.53%, specificity was 53.33%, and coincidence rate was 52.04%.CRP, PA, WBC combined diagnosis showed 62 cases were positive, 36 cases negative, 3 cases false positive, and 9 cases false negative, the detection sensitivity was 86.76%, specificity was 90.00%, and coincidence rate was 87.76%.The sensitivity, specificity and coincidence rate of CRP, PA and WBC combined diagnosis were significantly higher than those of CRP, PA and WBC diagnosis results.There were no significant differences in sensitivity, specificity and coincidence rate among single diagnosis of CRP, PA and WBC.Conclusion Implementation of combined CRP, PA with WBC in diagnosis of bacterial infection in children can effectively improve diagnostic coincidence rate.and provide theoretical basis for clinical treatment.

Objective To investigate and analyze the effect of two different doses of iopamidol enhancement on hepatic CT. Methods In Lishui people's hospital from January 2014 to August 201680 patients who accept hepatic enhancement scan were randomly divided into two groups, the study group and the control group, 40 cases in each group. The control group were received liver CT scan with iopamidol as contrast agent, the concentration was 300 g/L, the dosage was 1.5mL/kg, the concentration and dosage in the study group was respectively 300g/L and 1.2mL/kg. The enhancement effect after scanning in the two groups were observed and compared. Results The imaging quality in arterial phase and in portal phase in the study group were better than those in the control group (P<0.05); The adverse reactions in the study group were more than those in the control group, the difference between the two groups was statistically significant(P<0.05). Conclusion In liver CT scanning process, iopamidol as the contrast agent, the enhancement effect of 1.5mL/kg of iopamidol is better than 1.2mL/kg of iopamidol, but the efficiency scanning effect is improved at will also increase allergies and other adverse reactions..

As pattern recognition receptors of innate immunity,Toll-like receptors (TLRs) play an important role in the identification of pathogens,regulation of adaptive immunity and signal transduction.With more exploration of the mechanism of microbial keratitis and the acute or chronic rejection after penetrating keratoplasty,the induction and regulation of TLRs on the corneal immune response is increasingly concerned.Up to now,more and more studies of immune signal pathway of TLRs in mediating inflammatory response of bacterial keratitis,fungal keratitis,viral keratitis and graft rejection have been documented.To regulate the expression of TLRs is undoubtedly available for lightening and arresting the immuno-inflammatory response.Here,the current knowledge about TLRs,their signal transduction pathway and their regulation on corneal immunity are summarized,aiming to discuss their significant effects and the potential therapeutic targets.

Objective Experimental research demonstrated that oxidative damage leads to formation of cataract in rats and its machanism is the decline of activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-PX) and catalase(CAT) . Aaspirin can improve the antioxidative ability of lens. The purpose of this study was to observe the inhibition of aspirin on D-Galactose-induced cataractous lenses of rats. Methods Galactose cataract model was established in 40 cleaning Wistar rals by intraperitoneal injection of 20 mL/kg 80% D-Galactose for 10 days. The models were divided into model group (20 rats) and aspirin group(20 rats). 150 mg/kg of aspirin was administered immediately by gastrogavaging in aspirin group for 20 days. Other 20 normal Wistar rats were as control group. At day 3, 6, 10, 14, 20, the transparency of rat lenses was observed under the slit lamp microscopy. At day 5 after experiment, the ultrastructure of the lenses was examined and evaluated under the scanning electron microscopy. The activities of SOD, GSH-PX and CAT were detected by Coomassie Brilliant Blue color comparator, respectively. The use of experimental animal followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results All lenses were transparent in the rats of control group. The degree of lens opacity was more mild in asprin group compared with model group. 25. 00%, 41. 67%, 58. 33%, 83. 33% of lenses in aspirin group showed swelling at day 6, 10, 14, 20, respectively, but 65% lenses were opacity in model group on day 3 and 100% lenses were nuclear cataracts in 6 days. The structure of lenses was normal in control group, but the process number, fiber thickness and fiber density of lens were significantly increased in model group compared with control group (P ＜0. 05), and only process number was increased in asprin group. The activities of SOD, GSH-PX and CAT in lens of model group were obviously lower than in normal control group(P＜0. 05), but those in asprin group were significantly increased in comparison with model group(P ＜0. 05). Conclusion Aspirin could protect lenses of rats against oxidative damage by elevating activities of SOD, GSH-PX and CAT in lens and inhibiting the generation and development of galactose-induced cataract at early stage of cataract.

Background and purpose: MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many diseases. In this study, we explore the levels of miR-192 and zinc ifnger E-box binding homeobox 2 (ZEB2) in CRC, the clinical signiifcance of miR-192 and the correlation between the expression of miR-192 and ZEB2 protein. Methods: The expression levels of miR-192 and ZEB2 mRNA in 30 colorectal carcinoma samples, 30 corresponding cancer-adjacent tissue samples (from the edge of tumor≥5 cm), 25 colorectal adenoma samples, and 15 normal tissue samples were quantiifed using quantitative real-time polymerase chain reaction (qRT-PCR). ZEB2 protein expression was determined using Western blot. The relationship between the miR-192 and clinicopathological factors, miR-192 and ZEB2 protein expression was analyzed. Results:Signiifcant upregulation of miR-192 expression and reduction of ZEB2 mRNA and protein expression were identiifed in CRC tissues, compared to cancer-adjacent tissues, colorectal adenoma samples, and normal tissues (P<0.05). Low miR-192 levels were signiifcantly associated with lymph node (P=0.021) and distant metastasis (P=0.023). An inverse relationship between miR-192 and ZEB2 protein expression was identified in CRC group (r=-0.365, P<0.05). Conclusion: MiR-192 downexpression was correlated with CRC metastasis. MiR-192 may play a role in the development and progression of CRC through ZEB2.