OBJECTIVETo assess the effect of muscle contraction on bone capillary permeability.

METHODSThirty female New Zealand white rabbits were randomly divided into the experimental and control groups (n=15). The bone chambers were implanted medially into the right tibiae of the rabbit, and in the experimental group, the rabbits were stimulated by transcutaneous electrical nerve stimulator (TENS) at 4 Hz (1 h/day, 6 days/week) starting from week 2. From week 3, intravital microscopic observation was carried out weekly till week 10, and the vasculature in the tibiae was observed under fluorescent microscope with FITC and RITC staining. All the images were analyzed by Metamorph program.

RESULTSThe bone capillary permeability increased significantly in the experimental group.

CONCLUSIONSkeletal muscle contraction can increase the capillary permeability.

Animals ; Capillary Permeability ; physiology ; Female ; Implants, Experimental ; Muscle Contraction ; physiology ; Muscle, Skeletal ; physiology ; Rabbits ; Random Allocation ; Tibia ; blood supply ; surgery ; Transcutaneous Electric Nerve Stimulation

Objective To observe the stability of intraocular lens (IOL) in primary angle-closure glaucoma by ultra-long scan depth spectral domain optical coherence tomography (UL-OCT) after the phacoemulsification.Methods Totally 42 patients (42 eyes) with primary closed-angle glaucoma (PACG) and age-,gender-matched 40 patients (40 eyes) with age-related cataract were included in the study.All of patients underwent phacoemulsification combined with softec HD IOL implantation.Images were obtained by UL-OCT in 1 week,1 month and 3 months after the surgery for the calculation and analysis of the IOL decentration,tilt and the space between IOL and posterior capsule (IOL-PC space).And finally the intergroup differences at the different time-points were compared with the independent sample t test.Results The IOL decentration after 1 week,1 month and 3 months was (0.182 ± 0.054) mm,(0.232 ±0.081)mm,(0.183 ±0.089) mm in the PACG group,and (0.342 ± 0.141)mm,(0.288 ±0.110)mm,(0.249 ± 0.132)mm in the ARC group.There was statistically significant difference in IOL decentration 1 week after surgery between the two groups (P =0.006),but the differences in this variable at the other two time-points between the two groups were not statistically significant (both P ＞ 0.05).The tilt on the vertical axis at 1 week,1 month and 3 months after surgery was (3.670 ± 6.610) °,(-0.940 ± 4.730) °,(0.540±9.510)° in the PACG group,and (-4.090±7.610)°,(-5.100±5.530)°,(-1.430 ± 8.800) ° in the ARC group.The tilt on the vertical axis of the PACG group was less than that of the ARC group at 1 week after surgery,with statistical difference (P =0.025),but there was no significant difference in this variable at the other two time-points (both P ＞ 0.05).Moreover,the tilt on the horizontal axis showed no statistically significant differences (all P ＞ 0.05).The IOL-PC space on horizontal axis at 1 week,1 month and 3 months after surgery was (0.111 ±0.091)mm2,(0.044 ±0.066)mm2,(0.055 ±0.055) mm2 in the PACG group,and (0 176 ±0.213) mm2,(0.255 ±0.303) mm2,(0.059 ± 0.066) mm2 in the ARC group.The IOL-PC space on horizontal axis of the PACG group was less than that of the ARC group at 1 month after surgery,with statistical difference (P =0.045),but there was no significant difference in this variable at the other two time-points (both P ＞ 0.05).Moreover,the IOL-PC space on vertical axis showed no statistically significantdifferences (all P ＞ 0.05).Conclusion The stability of Softec IOL in the PACG group is better than that in ARC group in 3 months after phacoemulsification.

This study is to evaluate the therapeutic effect of fibroblast growth factor 21 (FGF21) on type 2 diabetic mice model and to provide mechanistic insights into its therapeutic effect. Type 2 diabetic animal model was established with high calorie fat diet and low dose streptozotocin (STZ) injection. Mice were then randomized into 5 groups: model control, FGF21 0.25 and 0.05 μmol x kg(-1) x d(-1) groups, insulin treatment group. Ten age-matched normal KM mouse administered with saline were used as normal controls. Serum glucose, insulin, lipid products and the change of serum and liver tissue inflammation factor levels between five groups of mouse were determined. The results showed that blood glucose, insulin, free fatty acids (FFAs), triglycerides, and inflammatory factor average FGF-21 of type 2 diabetes model group and normal control group were significantly higher (P < 0.01), while compared with insulin group, no difference was significant. Average blood glucose, insulin, blood lipid and inflammatory factor of FGF-21 treatment group compared with type 2 diabetes group was significantly lower (P < 0.01) and insulin group has no difference with the model control group. The results of OGTT and HOMA-IR showed that insulin resistance state was significantly relieved in a dose-dependent manner. Thus, this study demonstrates that FGF-21 significantly remits type 2 diabetic mice model's insulin resistance state and participates in the regulation of inflammatory factor levels and type 2 diabetes metabolic disorders.
Animals ; Blood Glucose ; Diabetes Mellitus, Experimental ; drug therapy ; Diabetes Mellitus, Type 2 ; drug therapy ; Diet, High-Fat ; Fatty Acids, Nonesterified ; blood ; Fibroblast Growth Factors ; pharmacology ; Insulin ; blood ; Insulin Resistance ; Mice ; Streptozocin ; Triglycerides ; blood

OBJECTIVETo evaluate the clinical outcomes of half intracytoplasmic sperm injection (partial ICSI) treatment in infertile patients with potential fertilization failure.

METHODSA total of 109 partial ICSI cycles of in vitro fertilization-embryo transfer (IVF-ET) were classified into 5 groups, namely group A (infertile patients for unidentified causes, 17 cycles), group B (oligo-asthenozoospermia patients, 28 cycles), group C (teratozoospermia patients, 8 cycles), group D (primary infertile patients without definite causes, 31 cycles), and group E (secondary infertile patients without definite causes, 25 cycles). The fertilization rate and normal fertilization rate after IVF and ICSI were compared between the groups.

RESULTSSignificant differences were found in the fertilization rate following conventional IVF and ICSI in group A (53.1-/+38.8% vs 72.2-/+34.1%) and group D (58.8-/+31.6% vs 82.7-/+21.4%) (P<0.05), but not in groups B, C and E (P>0.05). The normal fertilization rates following IVF and ICSI in groups A, B, D, E were statistically different (P<0.05), but similar in group C (P>0.05).

CONCLUSIONICSI treatment may increase the fertilization rate of IVF-ET in patients with unexplained infertility and primary infertility, but not in patients with oligo-asthenozoospermia, teratozoospermia or secondary infertility.

Adult ; Asthenozoospermia ; Embryo Transfer ; Female ; Humans ; Infertility, Male ; therapy ; Male ; Pregnancy ; Pregnancy Outcome ; Retrospective Studies ; Sperm Injections, Intracytoplasmic ; Spermatozoa ; abnormalities

Objective To investigate the effect of intraoperative renal artery resistance index(RI)in predicting postoperative acute kidney injury(AKI)in patients undergoing cardiac surgery with cardiopul-monary bypass(CPB).Methods Forty-four patients undergoing elective cardiac surgery with CPB,21 males and 23 females,aged ≥18 years,BMI 18.5-30.0 kg/m2,ASA physical status Ⅰ-Ⅲ,were select-ed.Left renal artery peak systolic velocity(PSV)and end-diastolic velocity(EDV)were measured by transesophageal echocardiography(TEE)20 minutes after general anesthesia induction(T1)and 30 minutes after CPB cessation(T2).RI was calculated as(PSV-EDV)/PSV.Patients were divided into two groups:the AKI group and the non-AKI group,according to whether occurred AKI by the diagnostic criteria of the kidney disease:improving global outcomes organization(KDIGO).Logistic multivariate regression analysis was performed to identify the risk factors for AKI occurrence.Results Sixteen patients(36％)oc-curred AKI.Compared with the non-AKI group,the CPB duration and aortic cross-clamp duration were sig-nificantly prolonged(P＜0.05),the renal artery RI at T,and T2 were significantly increased in the AKI group(P＜0.05).Multivariate logstic analysis showed that RI at T2 was an independent risk factor for AKI occurrence after cardiac surgery,the AUC was 0.893(95％CI 0.794-0.991,P＜0.010),the sensitivity and specificity were 84.5％and 78.6％,respectively,and the cut-off value was 0.720.Conclusion Intra-operative renal artery RI in patients undergoing cardiac surgery is an independent risk factor for AKI after cardiac surgery,and renal artery RI＞0.720 at 30 minutes after CPB cessation can serve as a predictive in-dicator for AKI after cardiac surgery.

Objective To observe area of coagulation necrosis after microwave ablation(MWA)of rabbit liver showed on contrast-enhanced ultrasound(CEUS)in comparison with pathological findings.Methods Twenty-five healthy male experimental rabbits were equally randomly divided into L0,M0,H0,M7 and M14 groups.MWA was achieved with different power and time,and then CEUS and pathological examinations were performed,respectively.Four target areas of rabbit liver were ablated under 20 W for 1 min in L0 group,under 30 W for 1 min in M0 group and under 30 W for 3 min in H0 group,and the ablated areas were observed on the same day after MWA,while 2 target areas of rabbit liver were ablated under 30 W for 1 min in M7 and M14 groups,and the ablated areas were observed 7 days and 14 days after MWA,respectively.The ablated foci and ablated foci+edema band(inflammatory reaction band,IRB)showed on CEUS and pathology were compared,and their differences and changes with time going were analyzed.Results On the day of ablation,the extent of ablation foci,ablation foci+IRB as well as IRB in L0,M0 and H0 groups showed on CEUS were all larger than pathological findings under light microscopy(all P＜0.05),and the difference value of the length of the ablation foci between 2 methods increased sequentially among group L0,M0 and H0(all adjusted P＜0.05).Seven days after ablation,the area of ablation foci measured with CEUS in M 7 group was slightly larger than that measured with pathology(adjusted P=0.045),but no significant difference of the length nor the short diameter was found(both adjusted P＞0.05).Fourteen days after ablation,no significant difference of the length,the short diameter nor the area of ablation foci was detected between 2 methods in M14 group(all adjusted P＞0.05).The length,short diameter and area of ablation foci in M0 group measured with CEUS or pathology were all smaller than those in M 7 and M14 groups(all adjusted P＜0.05),while no significant difference was found between the latter 2 groups(all adjusted P＞0.05).Conclusion On the day of MWA of rabbit liver with different power and time settings,the areas of ablation foci,ablation foci+IRB and IRB showed on CEUS were larger than pathological findings.The range of ablation foci showed on CEUS 7 days after ablation was basically in line with pathology,indicating that CUES should be reexamined 7 days after ablation to evaluate the actual extent of coagulation and necrosis of ablation foci.

[Abstrca t] Objective BRCA 1 ( breast cancer susceptibility gene 1) and RAP80 ( receptor-associated protein 80) play key roles in predicting chemosensitivity of platinum and taxanes .A randomized trial was carried out to compare non-selected cisplatin-based chemotherapy with therapy customized according to BRCA1 and RAP80 expression.Methods Advanced stage NSCLC patients whose tumor specimen was sufficient for molecular analysis were randomized (1∶3) to the control or experimental arm.Patients in the control arm received docetaxel/cisplatin; in the experimental arm , patients with low RAP 80 expression received gemcitabine/cisplatin ( Arm 1 ) , those with intermediate/high RAP 80 expression and low/intermediate BRCA 1expression received docetaxel/cisplatin ( Arm 2 ) , and those with intermediate/high RAP80 expression and high BRCA1 expression received docetaxel alone (Arm 3).The primary end point was progression-free survival (PFS).Results 226 patients were screened and 124 were randomized in this trial.ORR in the four subgroups was 22.6%, 48.4%, 30.3%and 19.2%, respectively (P=0.08);PFS was 4.74, 5.59, 3.78 and 2.73 months, respectively (P=0.55); and OS was 10.82, 14.44, 10.86 and 10.86 months, respectively (P=0.84).The common adverse effects included neutropenia , nausea, anemia and fatigue.Conclusions No statistically significant difference of ORR , PFS or OS is observed in the experimental arms compared with the control arm .Patients with low RAP 80 mRNA levels have a trend of better survival and higher response rate to gemcitabine /cisplatin chemotherapy .

[Abstrca t] Objective BRCA 1 ( breast cancer susceptibility gene 1) and RAP80 ( receptor-associated protein 80) play key roles in predicting chemosensitivity of platinum and taxanes .A randomized trial was carried out to compare non-selected cisplatin-based chemotherapy with therapy customized according to BRCA1 and RAP80 expression.Methods Advanced stage NSCLC patients whose tumor specimen was sufficient for molecular analysis were randomized (1∶3) to the control or experimental arm.Patients in the control arm received docetaxel/cisplatin; in the experimental arm , patients with low RAP 80 expression received gemcitabine/cisplatin ( Arm 1 ) , those with intermediate/high RAP 80 expression and low/intermediate BRCA 1expression received docetaxel/cisplatin ( Arm 2 ) , and those with intermediate/high RAP80 expression and high BRCA1 expression received docetaxel alone (Arm 3).The primary end point was progression-free survival (PFS).Results 226 patients were screened and 124 were randomized in this trial.ORR in the four subgroups was 22.6%, 48.4%, 30.3%and 19.2%, respectively (P=0.08);PFS was 4.74, 5.59, 3.78 and 2.73 months, respectively (P=0.55); and OS was 10.82, 14.44, 10.86 and 10.86 months, respectively (P=0.84).The common adverse effects included neutropenia , nausea, anemia and fatigue.Conclusions No statistically significant difference of ORR , PFS or OS is observed in the experimental arms compared with the control arm .Patients with low RAP 80 mRNA levels have a trend of better survival and higher response rate to gemcitabine /cisplatin chemotherapy .

OBJECTIVE: To observe the effect of electroacupuncture (EA) at sensitized acupoints on choline acetyltransferase positive (ChAT METHODS: A total of 79 male SD rats were randomized into five groups, i.e. a normal group (20 rats), a normal plus sensitized acupoint group (5 rats), a model group (34 rats), an EA RESULTS: The EB extravasating areas were distributed in the segments from T CONCLUSION The segmental dominance (acupoints) from T
Acupuncture Points ; Animals ; Cholinergic Neurons ; Colon ; Electroacupuncture ; Male ; Rats ; Rats, Sprague-Dawley