Aromatic hydrocarbons are capital environmental contaminations. Pseudomonas alcaligenes NCIB 9867 (P25X) is capable of degrading aromatic hydrocarbons via the gentisate pathway. Biochemical characterization indicated that it has isofunctional enzymes for the mono- and dioxygenase- catalysed reactions. One set of the enzymes is constitutive and the other is strictly inducible. To date, only the genes for the constitutively-expressed gentisate dioxygenase and the downstream enzymes have been cloned. A mutant strain of P25X, designated SNZ28, which had the constitutive copy of the gentisate 1, 2-dioxygenase gene interrupted by a streptomycin/spectinomycin resistance gene cassette, was found to still express gentisate dioxygenase when induced by gentisate. The proteome profiles of P25X and mutant SNZ28, grown in the presence and absence of the aromatic inducer gentisate, were compared after 2D-PAGE. Fifteen distinctive protein spots which were observed only in induced cells of P25X and mutant SNZ28 but absent in non-induced cells of both were further analyzed by MALDI-TOF and Q-TOF. Of the 15 proteins, 12 showed significant sequence similarity to proteins with assigned function in other microorganisms . The identification of protein P4 which showed positive identification to a gentisate dioxygenase from Ralstonia species indicated the putative role of this protein to encode gentisate 1, 2-dioxygenase in P.alcaligenes.

BACKGROUND:Studies have shown that overexpression of myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp) is the main reason for early neuronal regeneration failure. OBJECTIVE:To study the effect of human amniotic mesenchymal stem cel s (hAMSCs) transplantation on neural functional recovery of rats with ischemia/reperfusion (I/R) injury. METHODS:Sixty Sprague-Dawley were randomized into sham, I/R, and hAMSCs groups (n=20 per group). Rats in the hAMSCs group were given 1 mL of hAMSCs suspension (1.0×108/L) via the tail vein 24 hours after I/R injury. Rat neurological function recovery was assessed based on behavior changes, as determined by Longa behavioral score, cylinder test, horizontal ladder walking test and limb symmetry test at 1, 3 days, and 1, 2, 3 weeks post transplantation. Cel migration and distribution were observed using immunofluorescence method at 1 day, and 1, 2, 3 weeks post transplantation. MAG and OMgp protein expression was detected by western blot assay at 2 weeks post transplantation. Neuronal apoptosis was detected by TUNEL staining at 3 weeks post transplantation. RESULTS AND CONCLUSION:In the hAMSCs transplantation group, red marker-positive cel s were visible around the injury region at 1 week after transplantation, and over time, these cel s were increased in number. Significant improvement in the neurological function of rats were observed in the hAMSCs group as compared with the I/R group at 3 days and 1, 2, 3 weeks after transplantation (P<0.05), and the expression of MAG and OMgp proteins were also decreased dramatical y in the hAMSCs group (P<0.05). After I/R injury, the number of apoptotic cel s was increased, but hAMSCs transplantation reversed this effect. Overal , hAMSCs transplantation can reduce neuronal apoptosis by reducing MAG and OMgp expression levels, and thereby promote neurological functional recovery from I/R injury in rats.

OBJECTIVE: To summarize the approaches and methods for clinical pharmacists to provide clinical pharmaceutical care.METHODS: By reviewing pertinent literature combining the working experience of the clinical pharmacists,the approaches and methods for clinical pharmacists to provide pharmaceutical care were explored.RESULTS: Pharmacists could provide clinical pharmaceutical care by participating in clinical rounds,consultation and pharmaceutical monitoring,intervening medical order,offering drug-counseling service,handling the adverse clinical pharmacy events and providing medication education etc.CONCLUSIONS: Clinical pharmacists can provide various kinds of clinical pharmaceutical services for the safe,effective and economical use of drugs.

OBJECTIVE:To investigate the status quo and rationality of the perioperative use of drugs in patients with cataracta. METHODS:The drug use data of 60 patients undergoing cataracta surgery in our hospital from Apr. 2007 to Mar. 2008 were analyzed retrospectively and statistically. RESULTS:The drugs used during perioperative period consisted of antibacterials (applied either systemically or locally) and other assistant drugs (blood coagulants,drugs for decreasing intraocular pressure,anti inflammatory drugs and Chinese patent medicines). All the patients were treated with systematic antibacterials for prevention medication. The systemically used antibacterials consisted of ?-lactam antibiotics,clindamycins,aminoglycosides,fluoroquinolones and amphemycins. The problems existed in systemic use of the antibacterials manifested as irrationality in the choice of drug category,dosage,administration frequency,administration time and drug combination,etc. The locally applied antibacterials consisted of aminoglycosides,fluoroquinolones,tetracyclines and lincomycins. The problems existed in the local application of the antibacterials manifested as irrationality in administration time,drug change and drug combination,etc. CONCLUSION:The rationality of the perioperative drug use in patients with cataracta in our hospital remains to be improved.

OBJECTIVE:To analyze the problems existing in the use of thiamphenicol for outpatients. METHODS:In this retrospective study,a total of 743 outpatient prescriptions of Thiamphenicol enteric-coated tablets in one hospital during Mar. 2008 were analyzed in respect of the prescription writing,medication suitability and amount of money of prescriptions,etc. RESULTS:The problems in the use of Thiamphenicol manifested as nonstandard in prescription writing,irrational in clinical drug choice,drug combination,administration and dosage,etc. CONCLUSION:The use of Thiamphenicol in the hospital is somewhat irrational thus remains to be standardized further.

Cooperative Behavior ; Humans ; Multicenter Studies as Topic ; Pancreatic Neoplasms ; therapy ; Quality Control ; Research

Objective To detect the methylation status of CDH1 3 gene promoter in colon cancer by using methylation-specific polymerase chain reaction(MSP)technique.Method The tissue specimens from 32 cases of colon cancer(observation group),and 12 cases of normal colon tissues(control group)were examined,the methylation of CDH13 gene promoter was detected by MSP.Result The methylation of CDH13 gene promoter was detected in 19 cases(59.4％,19/32)in observation group,1 case(8.3 ％,1 / 12)in control group,there was statistical significance between two groups(P =0.002).Conclusion Frequency of the methylation of CDH13 gene promoter is apparently higher in colon cancer tissues than that in normal colon tissues,it reveals that CDH13 gene promoter may contribute significantly to the development of colon cancer.

Using registration information on candidates for the written examination in medical synthesis, a part of the doctor qualification examination, from 1999 to 2001 as processing data, the authors made a comparative study on the composition of the candidates in the eastern, middle and western regions of the country from 7 aspects, viz. total number, category of registration for the examination, educational background, time of graduation, age, organization and rank of organization. It was found that the educational background of the candidates was on the whole very low and the number and quality of health personnel in the western region and at the grass roots level was in dire need of enhancement.

Objective To study the effect of short-term inte ns ive insulin treatment on the pancreatic B-cell function in type 2 diabetic mell itus (T2DM).Methods Insulin and C-peptide serum levels were measured befor e and after intensive insulin treatment in T2DM by chemical luminescence immunoa ssay.Results The levels of insulin and C-peptide were increased at 0、30、60、120、180min in OGTT in T2DM after insulin intensive treatment.Conclusion Short-term intensive insulin treatment can partiall y improve the B-cell function of T2DM with lower function and made it rest.