Objective To study the diagnosis and treatm ent of choledochoduodenal Fistula (CDF).Methods To analysis preoperative and operative examination and postoperative situation of CDF.Results The operation confirmed the preoperative ERCP diagnosis of 19 cases with CDF.9 cases have been taken the choledochojejunostomy with tran sverse cutting the common bile duct and this method has been proved very effecti ve. Conclusions ERCP is an important method for the preoper ative diagnosis of CDF. The choledochojejunostomy with the common bile transvers ed cat duct is a recommended procedure to CDF.

Epstein-Barr virus (EBV) is a biological factor associated with gastric carcinoma. The prognosis of EBV-associated gastric car-cinoma (EBVaGC) is good because of its distinct clinicopathological features. The pathogenesis mechanism of EBVaGC is extensively in-vestigated at present. The development of molecular techniques has stimulated the research on the expression of EBV genes in EB-VaGC, thereby providing a theoretical foundation on the diagnosis, therapy, and prevention of EBVaGC. This article reviewed present research advances in EBV genes in EBVaGC.

Nasopharyngeal carcinoma (NPC) is prevalent in Southeast Asia, especially in the area of southern China. It is acknowl-edged that NPC is absolutely related to EBV infection. Nevertheless, there are just a few researches about the relationship between the level of EBV antibodies and the tumor stages and it hasn't come to conclusion. This review makes a summary on present research progress and would be valuable for the better understanding of the contribution of EBV antibodies to the tumor stages in NPC.

Microbes that produce Docosahexaenoic Acid were isolated from seawater. 160 strains capable of producing lipids were screened out using Sudan Black B dying method from 280 seawater samples. From 60 strains of microorganisms producing bigger lipid particles, 7 strains of them capable of producing lipids more than 8% were obtained with Soxhlet abstracting method in the first screening. In the secondary screening from 10 strains with high lipids yield, strain 7-3 capable of producing 15.9% lipids was obtained, in which the content of DHA(Docosahexaenoic Acid)is 45.2%. Strain 7-3 was identified as Brettanomyces based on its morphological properties, cultural characteristics, physiological and biochemical properties.

Objective To investigate expression and suppressive function of CD39 + regulatory T cells (Treg) in kidney transplant recipients.Method Thirty recipients of first kidney transplants were treated with tacrolimus,mycophenolate mofetil and prednisone.Within 28 days posttransplantation,there were 14 patients subject to acute rejection (AR group),and the rest 16 patients had no episodes of acute rejections (NR group).Twelve healthy volunteers served as healthy controls (HC group).We collected peripheral blood from the three groups and separated PBMC by density gradient centrifugation,and sorted Tresp,CD39-Treg and CD39+ Treg by flow cytometry.We next analyzed the ratio of CD39 + Treg/CD4+ T cells.ELISA was used to determine the suppressive ability of CD39-Treg and CD39+ Treg on secretion of IFN-γ and IL-17 by Tresp.Results The ratio of CD39 + Treg/CD4 + T cells in AR group was significantly reduced as compared with HC group and NR group (P＜0.05).In HC group and NR group,the secretion of IFN-γ and IL-17 by Tresp was suppressed significantly (P＜0.05) by CD39+ Treg.CD39Treg could suppress secretion of IFN-γ but not IL-17 production by Tresp.CD39+ Treg in AR group AR could suppress the secretion of IFN-γ significantly (P＜0.01),but not to IL-17 production.Conclusion CD39+ Treg have important immunoregulation function.The relative amount of CD39+ Treg was reduced and their regulatory function was impaired in patients with acute rejection.

Objective To explore the outcomes of kidney transplant recipients who developed urinary and male genital cancers after transplantation. Methods Data of 31 kidney transplant recipients developed de novo urinary and male genital cancer were compared with data of 31 patients in general population with the same age and same tumor stage. Results Compared with the general population, the overall survival was significantly worse in the transplant recipients (P=0. 02) , 5-year survival rates for each group were 50% vs 68%. Multivariate analyses demonstrated cancer stage to be a negative risk factor for survival for transplant recipients with de novo urinary and male genital cancer, and surgery and functioning graft to be the positive survival predictors. Conclusions Transplant recipients experience worse outcomes than the general population from urinary and male genital cancers. Cancers in transplant recipients are more biologically aggressive at the time of diagnosis.

OBJECTIVETo explore the relationship of plasma endotoxin level with TCM deficiency/excess Syndrome and the grading/staging of pathologic change of liver in patients with chronic hepatitis B (CHB).

METHODSPlasma level of endotoxin (ET) was determined using chromogenic limulus amebocyte lysate (LAL) assay in 75 patients with chronic hepatitis B (30 of excess dominance, 45 of deficiency dominance) and also in 15 healthy subjects for control. Moreover, pathological examination on liver biopsy of 20 patients was conducted.

RESULTS(1) ET level increased in all CHB patients, and the increment was higher in patients of excess dominance than that in patients of deficiency dominance, showing significant difference (P < 0.01). (2) Correlation between ET level and liver pathological grading/staging was shown in CHB patients.

CONCLUSIONPlasma level of ET could be taken as one of the criteria of TCM microcosmic diagnosis in differentiating deficiency/excess syndrome. (2) Endotoxemia plays an important role in pathologic process of liver injury.

Adolescent ; Adult ; Aged ; Diagnosis, Differential ; Endotoxins ; blood ; Female ; Hepatitis B, Chronic ; blood ; etiology ; pathology ; Humans ; Liver ; pathology ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Yang Deficiency ; blood ; pathology ; Yin Deficiency ; blood ; pathology

Animals ; Animals, Newborn ; Bronchoalveolar Lavage Fluid ; chemistry ; cytology ; Female ; Lipopolysaccharides ; toxicity ; Lung ; drug effects ; enzymology ; pathology ; Male ; Neutrophils ; cytology ; Peroxidase ; analysis ; Rats ; Rats, Wistar

Objective To explore the effects of cyclosporin A (CsA) and tacrolimus (Tac) on biological behaviors of lung cancer A549 cells in nude mice.Methods Thirty-six models of transplanted tumor in Balb/c mice were established by using lung cancer A549 cells and divided into three groups:control group,without given any immunosuppressant; CsA group,intraperitoneally given CsA; Tac group,intraperitoneally given Tac.The transplanted tumor growth curve was drawn according to the transplanted tumor volume,and the influencing ratio was calculated according to the final tumor weight.The changes in cell migration ability were observed by using Transwell system.Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) assay was used to examine the apoptosis index of the transplanted tumor.Quantitative RT-PCR was used to detect the expression levels of Bcl-2 mRNA and Bax mRNA.Results The growth of transplanted tumor in CsA and Tac groups was faster than in control group.Final tumor volume and final tumor weight in CsA and Tac groups were greater than those in control group.The influencing ratio in CsA and Tac groups was 19％ (P＜0.05) and 25％ (P＜0.05),respectively.The migration ability was greater in CsA and Tac groups than in control group (P＜0.01).The apoptosis index of the transplanted tumor in CsA and Tac groups was lower than in control group (P＜0.05).The expression level of Bcl-2 mRNA was higher in CsA and Tac groups than in control group (P＜0.05),and that of Bax mRNA was lower in CsA and Tac groups than in control group (P＜0.05).Conclusion Both CsA and Tac can promote the growth of transplanted tumor in nuce mice bearing 549 cells and enhance the invasion forces,which is probably related with the apoptosis induction of tumor cells.

Objective:To remove murine embryonic stem cells(mESC)from the differentiating cell culture and purify the differentiated cells by Magnetic Activated Cell Sorting(MACS).Methods:Neural differentiation of mESC was induced by a 5-stage method.The specific cell surface marker,SSEA-1,was used to identify ES cells in the differentiating cells.The optimal dilutions of mouse anti mouse SSEA-1 IgM primary antibody and FITC conjugated goat anti mouse secondary antibody were determined before the flow cytometry test.The incubation time and incubation temperature of primary antibody were all optimized to make the cytometry test accurate.After the optimization,stage 4 cells were dissociated into single cell suspension,incubated with antibody of SSEA-1 and microbeads conjugated goat anti mouse IgM,and then sorted through the magnetic field.The rate of SSEA-1 positive cells in pre-and post-separation groups was assessed by flow cytometry,and the viability of cells was evaluated by trypan blue staining counting under light microscopy.Results:The proportion of SSEA-1 positive cells in the separated cells can be reduced from(7.19?1.36)% to(1.34?0.80)%.The survival rate of sorted cells was more than 92%,similar to that of pre-separation cells.Conclusions:The MACS system we used can effectively remove mESC from the differentiated cells.The sorted cells will be well provided for the subsequent studies about transplantation therapy.