Objective To investigate the clinical features, BCL-2,CMYC protein expression and prognosis of patients with hepatitis B virus-associated diffuse large B-cell lymphoma (DLBCL).Methods A retrospective study was used to analyse 94 diagnosed DLBCL patients clinical data.Immunohistochemistry method was used to detect the expression of BCL-2 and CMYC protein in paraffin sections of tumor tissues, and to analyze the clinical features, protein expression and prognosis of patients with hepatitis B virus-associated DLBCL.Results ① The rate of HBV infection in the 94 DLBCL patients was 27.66%, significantly higher than the general population(7.18%).Compared with HbsAg-negative DLBCL, HbsAg-positive DLBCL displayed more advanced disease(P=0.032), higher international prognostic index (IPI)(P=0.047) and more frequent involvement of spleen(P=0.02).There were no significant differences in gender distribution, age, immunological subtype and treatment between the two groups.② The aspect of BCL-2 and CMYC protein expression between the two groups: HbsAg-positive group BCL-2 protein expression was significantly higher than HbsAg-negative group (84.6% vs 58.8%, P=0.018).There were no significant differences on the CMYC protein expression and CMYC/ BCL-2 double expression.③ Univariate survival analysis showed that hepatitis B virus infection, BCL-2 positive, CMYC positive, CMYC/ BCL-2 double expression, IPI high-risk group were associated with unfavorable prognostic of overall survival time (OS) and progression free survival (PFS).Cox multivariate analysis showed that CMYC/BCL-2 double expression, IPI high-risk group were independent adverse prognostic factors for OS and PFS.Conclusion HBV infection, BCL-2, CMYC, BCL-2/CMYC double expression, IPI are related to the prognosis of DLBCL.HBV infection may affect the development and prognosis of DLBCL by regulating the expression of BCL-2.

Objective To evaluate the utilization status of essential medicines at community health centers,and analyze problems existing in the stocking and application situation of such medicines,hence to provide references and suggestions for the implementation of the Essential Medicine System.Methods The investigation method was used to analyze the actual stocking situation of essential medicines at community health centers,and existing problems in implementing the Essential Medicine System.Results An average of 377 species of essential medicines were stocked by the community health institutions, accounting for 53.9% of Beijing Essential Medicine List,and average 61.6% of the total medicines in these institutions.There were insufficiencies existing both in the list and supplies of essential medicines. First-aid and low-price essential medicines are out of stock.Lack of confidence by some patients on the drug brands and their quality covered in the essential medicines.Conclusions The list and supplies of essential medicines could not meet the demand of patients at community health centers adequately.The method of evidence-based practice should be applied to each area in implementing of the Essential Medicine System.

Adult ; Cryptococcosis ; physiopathology ; Cryptococcus neoformans ; Duodenal Diseases ; microbiology ; physiopathology ; Humans ; Male

Antineoplastic Agents ; adverse effects ; Humans ; Integrative Medicine ; Organoplatinum Compounds ; adverse effects ; Peripheral Nervous System Diseases ; chemically induced

Objective To explore the early diagnosis and treatment of Van Wyk-Grumbach syndrome (VWGS). Methods The clinical data of a child with VWGS were retrospectively analyzed. The related literatures were reviewed. Results Nine-year-old female presented with growth retardation and obesity for 3 years, combined with breast development for 6 months and vaginal bleeding for 3 month. The level of free thyroxine (FT4) was 0.46 ng/dL and thyrotropin (TSH)>150 mIU/L. The GnRH stimulation test showed that the gonad axis was not activated. The serum prolactin and estradiol were significantly increased. Bone age was delayed. Color Doppler ultrasound showed enlarged uterus and enlarged ovary, and ovarian cyst was seen. Pituitary MRI showed hyperplasia of the pituitary gland. The patient received the treatment of Euthyrox, and 2 months later, thyroid function was back to normal, ovaries were significantly reduced by reexamine of color doppler ultrasound, body weight was reduced by 6 kg, and there was no vaginal bleeding. Three months later, both ovaries returned to normal size, and pituitary MRI showed hyperplasia of adenohypophysis was improved. After 6 months, both of uterus and ovary were turn to normal size, ovarian cyst disappeared, and pituitary MRI showed normal. Conclusions VWGS is a rare complication of severe primary hypothyroidism untreated for long time and it mainly occurs in adolescent girls. Thyroid replacement therapy is effective.

Objective:To evaluate the in vitro and in vivo immunostimulatory activity and the safety of ethanol extract of wild Artemisia rupestris L. (EEWAR). Methods:Bone marrow dendritic cells (BMDCs) from C57BL/6 mice were treated with different concentrations of EEWAR in vitro and the expression of CD40 and CD80 on BMDCs was detected by flow cytometry. ICR mice were subcutaneously immunized with different concentrations of EEWAR in combination with ovalbumin (OVA) or OVA alone. Aluminum adjuvant was used as the positive control. OVA-specific IgG antibodies in mouse serum samples were measured by ELISA following immunization. T cell proliferation in spleen tissues was detected by MTT method. Acute toxicity test was conducted in ICR mice to analyze the safety of EEWAR. Results:In vitro experiment showed that EEWAR at the concentrations of 10-20 μg/ml increased the expression of CD40 and CD80 on BMDCs ( P<0.05), and had no significant effect on the morphology of BMDCs; EEWAR at the concentrations of 100-200 μg/ml significantly promoted the expression of CD40 and CD80 on BMDCs ( P<0.01), but had a certain influence on the morphology of BMDCs. In vivo experiment showed that EEWAR enhanced the production of IgG, IgG 1 and IgG 2a antibodies against OVA and the proliferation of splenocytes ( P<0.05). In the acute toxicity test, EEWAR at the concentrations of 50-5 000 μg/ml had no side effects on mouse body weight and was relatively safe. Conclusions:EEWAR could promote the maturation of DCs and enhance the humoral and cellular immune responses when used as an adjuvant to OVA. It was safe in a certain dose range. This study provided reference for further research on EEWAR as a new-generation adjuvant.

3) in control group (χ2=5.42,P=0.02).Conclusions Gemcitabine chemotherapy combined with HIFU treatment was more effective for pancreatic tumor growth control and pain relief without more adverse events,which was effective and safe.

To investigate the chemical constituents of hemostatic extract of Pyrola calliantha H. Andres, the extract was subjected to chromatographic separation and purification. Along with some known compounds, a new naphthaquinone derivative was isolated and identified as 2-(1, 4-dihydro-2, 6-dimethyl-1, 4-dioxo-3-naphthalenyl)-3, 4, 5-trihydroxylbenzoic acid by physicochemical and spectroscopic analysis.

Objective To investigate the properties of hind foot motion and it's changes in ankle or subtalar joint arthrodesis. Methods Eight fresh frozen adult leg-foot specimens were mounted in a coordinated system, a Steinmann pin was perforated across the calcaneus as a marker. The displacements of the marked points in the coordinated system were recorded when subtalar or ankle joint was fixed respectively. Through matrix translation and Euler equation, the angle of the calcaneus rotation were calculated, representing the motion of the subtalar and ankle joints in different condition, Statistical analysis were also taken between different conditions. Results Three dimensional (3-D) motion was observed in hind foot motion. The range of motion with ankle joint fixation was: inversion 15.99??2.81?, eversion 8.62??1.90?, dorsiflexion 3.70??1.00?, plantar flexion 5.87??1.39?, adduction 7.64??2.04?, and abduction 3.99??1.78?. When subtalar joint was fixed, they were: 7.13??1.07?, 5.52??0.85?, 17.09??2.87?, 30.75??5.04?, 10.77??1.56?, and 6.54??1.88? respectively. While they were 22.62??3.21?, 40.88??5.77?, 18.87??3.89?, 9.12??2.05?, 17.48??2.26? and 11.47??2.29?, when no joint was fixed. Arthrodeses of ankle or subtalar joint would affect the hindfoot motion significantly (P

Objective:To assess the quality of life (QOL) and cognition function in patients with severe obstructive sleep apnea (OSAHS), and to study the relationship between the QOL and severity of OSAHS, excessive daytime sleepiness (EDS),cognition disorders. Methods:Sixty-two patients with OSAHS and 32 non-OSAHS patients (control group) which all diagnosed by polysomnography (PSG) were chosen from The First Hospital of Lanzhou University, and they were assessed by QOL using the Short-Form 36 Health Survey questionnaire (SF-36), by EDS using the Epworth sleepiness scale (ESS), and for cognition disorders by mini-mental state examination (MMSE).The associations between the total score on the SF-36 and the baseline characteristics, PSG parameters, such as ESS score, and MMSE score were examined by simple regression analysis and stepwise multiple regression analysis.Results:The scores of SF-36 and MMSE in OSAHS group were significantly lower than the control group (63.9?14.7/78.8?4.5, 24.6?2.0/27.1?1.3,P