1.Study on the Inhibitory Effect of Ligustrazine on the Formation of Macrophage-derived Foam Cells
China Pharmacy 2015;(22):3096-3098
OBJECTIVE:To investigate the inhibitory effect of ligustrazine on the formation of macrophage-derived foam cells and its possible mechanism. METHODS:Human acute mononuclear cells (THP-1) were incubated with 160 nmol/L phorbol ester (PMA) for 24 h to differentiate into macrophages;and the macrophages were incubated with oxidized low-density lipoprotein (ox-LDL)culture solution containing 80 mg/L for 24 h to differentiate into macrophage-derived foam cells. And then the cells were randomly divided into blank control group(ox-LDL),model(AngⅡ)group,positive control(valsartan)group,and ligustrazine low,medium and high concentration groups(the mass concentration were 0.025,0.05 and 0.1 g/L). After all cells were respective-ly incubated with 80 mg/L ox-LDL culture solution for 48 h,oil red O staining was adopted to observe the transformation rate of foam cells,enzyme chemical method was used to determine the content of cholesterol,and real-time quantitative polymerase chain (RT-PCR)and Western blot were conducted to detect expression levels of Acyl-coenzyme A cholesterol acyltransferase-1(ACAT-1) mRNA and its protein. RESULTS:Compared with blank control group,the transformation rate of foam cells and content of choles-terol in model group were increased,and the expression levels of ACAT-1 mRNA and its protein were obviously strengthened,with significant differences(P<0.01 or P<0.05). Compared with model group,the transformation rate of foam cells and content of cho-lesterol in positive control group(valsartan)and ligustrazine low,medium and high concentration groups were decreased,and the expression levels of ACAT-1 mRNA and its protein were obviously weakened,with significant differences (P<0.01 or P<0.05). CONCLUSIONS:Ligustrazine can inhibit the macrophages differentiating into foam cells,by a mechanism that may be related to inhibiting expression of ACAT-1,and reducing content of cholesterol to reduce formation of foam cells.
2.Preparation and Quality Control of Rapid-disintegrating Oral Tablets of Indometacin
China Pharmacy 2007;0(31):-
OBJECTIVE:To prepare rapid-disintegrating oral tablets of indometacin and establish a method for its quality control.METHODS:The formula of the oral tablets was optimized by orthogonal test with the formula quality of CMS-Na(A),L-HPC(B),MCC(C),manniton(D) as factors,and the disintegrating time as index.The content of indometacin was determined by ultraviolet spectrophotometry.RESULTS:The optimal formula of the tablets was the following,A∶B∶C∶D=5∶5∶50∶5.The preparation was white in color and its quality was up to the related standard specified in Chinese Pharmacopeia (2005 edition) in identification.The linear range of indometacin was 2.08~41.6 ?g?mL-1 (r=0.999 9).The average recovery was 99.3%.The intra-day RSD=0.53% and inter-day RSD=0.74%.CONCLUSION:The preparation is simple and feasible in preparation process,and controllable in quality.
3.Hospital Infection in Infectious Disease Ward with PDCA
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To discuss the hospital infection management in infectious disease ward,to improve the ward quality of hospital infection,and to prevent and control infection inside hospital.METHODS By the plan,do,check and action(PDCA) circle method to collect information,analyze cause,establish plans,and organize to put into practice and check management.RESULTS Making the control quality of hospital infection improved obviously,various index signs attain the Hospital infection management norm and The disinfection technique norm issued by Ministry of Health and infection managment standard requirement for third class grade A hospitals.CONCLUSIONS Inducting PDCA circle method and step by step to insist on it is a good method to improve the control quality of hospital infection.
7.An intervention to reduce discrimination against people living with HIV/AIDS among medical students
Chinese Journal of AIDS & STD 2006;0(02):-
Objective To explore the effectiveness of short-term integrated intervention to reduce discrimination against people living with HIV/AIDS(PLWHA)among medical students.Methods A quasi-experimental survey was conducted.Two medical schools in Beijing,one as intervention group and the other as control group were selected by convenience sampling,from which 123 undergraduate students or interns of medical and nursing specialties were recruited,62 in the intervention group and 61 in the control group.The intervention group was provided with 13 hour long antidiscrimination intervention by increasing HIV/AIDS related knowledge,sympathy and skill in dealing with people living with HIV/AIDS,while the control group was deprived of any intervention.The attitude of the students in the 2 groups were assessed by questionnaire survey in 5 time points after the intervention.Results Compared with the control group,at 4 time points after the intervention students in the intervention group had less discrimination against PLWHA in general;and significantly less discrimination in dealing with them as patients(P
8.One case of postoperative nursing care for patient undergoing domino liver transplantation with two living donors
Chinese Journal of Practical Nursing 2011;27(29):49-50
Objective To probe into the methods of postoperative nursing care for the patient undergoing domino liver transplantation.Methods The key of postoperative care included:monitoring of vital signs,the condition of cruor and the state of drainage rigorously; keeping the balance of intake and output; taking the immunosuppressive drugs correctly; proper diet; prevention of complication;giving psychological nursing.Results The patients passed the perioperative period successfully and were all cured.Conclusions To aim at the characteristic of this operation,implement holistic nursing care,is an important link to assure the patients of domino liver transplantation to recover smoothly after the operation.
9.Perioperative anticoagulant therapy in the prevention of deep vein thrombosis after rectal carcinoma surgery
Chinese Journal of General Surgery 2010;25(9):744-747
Objective To evaluate curative effect and safety of anticoagulant drugs used in perioperative period to prevent deep vein thrombosis (DVT) after rectal carcinoma surgery. Methods Clinical data of 96 postoperative rectal carcinoma cases from January 2006 to June 2009 were analyzed retrospectively.These cases were divided into two groups:48 cases in nonanticoagulant group and 48 cases in anticoagulant group.After radical operation of rectal carcinoma,low molecular heparin calcium was injected subcutaneously daily for 8 consecutive days beginning the day before surgery in anticoagulant group. Results Incidence of DVP in anticoagulant group (4%) was significantly lower than that in noncoagulant group (19%) (x2 = 5.031,P < 0.05).Postoperative hospitalization time in anticoagulant group [(12 ± 3) d] was significantly shorter than that of nonanticoagulant group [(22 ± 6) d] (t = 10.328,P< 0.01).There were no significant differences between two groups in operative time,blood loss and postoperative blood drainage,peripheral blood platelet counts before and after operation,prothombin time and active part thrombin time before and after operation,the time when postoperative DVT developes;secondary adverse reactions; postoperative major complication rate and mortality (all P > 0.05). Conclusion Anticoagulant drugs used in perioperative period is safe and effective in prevention of deep vein thrombosis in lower limbs after radical operation of rectal carcinoma.
10.Application value of endoscopy in the diagnosis and treatment of patients with gastrointestinal bleeding
Chinese Journal of Primary Medicine and Pharmacy 2017;24(9):1405-1408
Objective To explore the application value of endoscopy in the diagnosis and treatment of patients with gastrointestinal bleeding.Methods The clinical data of 74 patients with digestive tract hemorrhage underwent gastroscopy and treatment were retrospectively analyzed.The effect of gastroscopy was compared at different time and different treatment methods.Results Endoscopic examination showed 23 cases of duodenal ulcer disease (31.08%),including 15 cases of gastric ulcer(20.27%),10 cases of acute gastric mucosal lesion (13.51%),8 cases of gastric cancer(10.81%),gastrointestinal complex ulcer in 7 cases (9.46%),4 cases of gastritis (5.41%),anastomotic ulcer or erosion in 4 cases (5.41%),no lesions in 3 cases (4.05 %).71 cases were clearly diagnosed by gastroscopy,the detection rate was 95.95%,the hemorrhage in 48h positive rate was 100.00%,which was significantly higher than 87.50% of the hemorrhage 48h detection rate (x2 =13.33,P < 0.05).71 patients received endoscopic treatment,67 patients were successful,the success rate was 94.37%,the injection of hemostatic success in 16 cases (94.12%),spraying hemostasis successfully in 51 cases (94.44%),there was no significant difference (x2 =-0.01,P > 0.05).Conclusion In the patients with gastrointestinal bleeding,gastroscopy has advantages of high diagnostic accuracy,accurate curative effect,can shorten the bleeding time,improve the success rate of hemostasis,improve the prognosis,with high clinical value.