OBJECTIVE:To investigate the inhibitory effect of ligustrazine on the formation of macrophage-derived foam cells and its possible mechanism. METHODS:Human acute mononuclear cells (THP-1) were incubated with 160 nmol/L phorbol ester (PMA) for 24 h to differentiate into macrophages;and the macrophages were incubated with oxidized low-density lipoprotein (ox-LDL)culture solution containing 80 mg/L for 24 h to differentiate into macrophage-derived foam cells. And then the cells were randomly divided into blank control group(ox-LDL),model(AngⅡ)group,positive control(valsartan)group,and ligustrazine low,medium and high concentration groups(the mass concentration were 0.025,0.05 and 0.1 g/L). After all cells were respective-ly incubated with 80 mg/L ox-LDL culture solution for 48 h,oil red O staining was adopted to observe the transformation rate of foam cells,enzyme chemical method was used to determine the content of cholesterol,and real-time quantitative polymerase chain (RT-PCR)and Western blot were conducted to detect expression levels of Acyl-coenzyme A cholesterol acyltransferase-1(ACAT-1) mRNA and its protein. RESULTS:Compared with blank control group,the transformation rate of foam cells and content of choles-terol in model group were increased,and the expression levels of ACAT-1 mRNA and its protein were obviously strengthened,with significant differences(P<0.01 or P<0.05). Compared with model group,the transformation rate of foam cells and content of cho-lesterol in positive control group(valsartan)and ligustrazine low,medium and high concentration groups were decreased,and the expression levels of ACAT-1 mRNA and its protein were obviously weakened,with significant differences (P<0.01 or P<0.05). CONCLUSIONS:Ligustrazine can inhibit the macrophages differentiating into foam cells,by a mechanism that may be related to inhibiting expression of ACAT-1,and reducing content of cholesterol to reduce formation of foam cells.

OBJECTIVE To discuss the hospital infection management in infectious disease ward,to improve the ward quality of hospital infection,and to prevent and control infection inside hospital.METHODS By the plan,do,check and action(PDCA) circle method to collect information,analyze cause,establish plans,and organize to put into practice and check management.RESULTS Making the control quality of hospital infection improved obviously,various index signs attain the Hospital infection management norm and The disinfection technique norm issued by Ministry of Health and infection managment standard requirement for third class grade A hospitals.CONCLUSIONS Inducting PDCA circle method and step by step to insist on it is a good method to improve the control quality of hospital infection.

OBJECTIVE:To prepare rapid-disintegrating oral tablets of indometacin and establish a method for its quality control.METHODS:The formula of the oral tablets was optimized by orthogonal test with the formula quality of CMS-Na(A),L-HPC(B),MCC(C),manniton(D) as factors,and the disintegrating time as index.The content of indometacin was determined by ultraviolet spectrophotometry.RESULTS:The optimal formula of the tablets was the following,A∶B∶C∶D=5∶5∶50∶5.The preparation was white in color and its quality was up to the related standard specified in Chinese Pharmacopeia (2005 edition) in identification.The linear range of indometacin was 2.08～41.6 ?g?mL-1 (r=0.999 9).The average recovery was 99.3%.The intra-day RSD=0.53% and inter-day RSD=0.74%.CONCLUSION:The preparation is simple and feasible in preparation process,and controllable in quality.

Cell Line, Tumor ; Cell Proliferation ; drug effects ; Flow Cytometry ; methods ; Humans ; Point Mutation ; Stomach Neoplasms ; genetics ; pathology ; Tumor Suppressor Protein p53 ; genetics ; pharmacology

Acupuncture Therapy ; Adult ; Female ; Humans ; Lumbosacral Region ; injuries ; Male ; Middle Aged ; Sprains and Strains ; therapy ; Young Adult

Combined Modality Therapy ; Early Diagnosis ; Humans ; Mouth Neoplasms ; diagnosis ; therapy

Adult ; Biopsy ; Donor Selection ; Humans ; Liver ; pathology ; Liver Transplantation ; adverse effects ; pathology ; Living Donors ; Postoperative Complications

Objective To evaluate curative effect and safety of anticoagulant drugs used in perioperative period to prevent deep vein thrombosis (DVT) after rectal carcinoma surgery. Methods Clinical data of 96 postoperative rectal carcinoma cases from January 2006 to June 2009 were analyzed retrospectively.These cases were divided into two groups:48 cases in nonanticoagulant group and 48 cases in anticoagulant group.After radical operation of rectal carcinoma,low molecular heparin calcium was injected subcutaneously daily for 8 consecutive days beginning the day before surgery in anticoagulant group. Results Incidence of DVP in anticoagulant group (4%) was significantly lower than that in noncoagulant group (19%) (x2 = 5.031,P ＜ 0.05).Postoperative hospitalization time in anticoagulant group [(12 ± 3) d] was significantly shorter than that of nonanticoagulant group [(22 ± 6) d] (t = 10.328,P＜ 0.01).There were no significant differences between two groups in operative time,blood loss and postoperative blood drainage,peripheral blood platelet counts before and after operation,prothombin time and active part thrombin time before and after operation,the time when postoperative DVT developes;secondary adverse reactions; postoperative major complication rate and mortality (all P ＞ 0.05). Conclusion Anticoagulant drugs used in perioperative period is safe and effective in prevention of deep vein thrombosis in lower limbs after radical operation of rectal carcinoma.

Objective To systematically review the effects of air or liquid in a loss of resistance technique for identification of the epidural space. Methods We searched the Cochrane Library, PubMed, EMBASE, Chinese Biomedical Database (CBM), CNKI, VIP and ChinaInfo for studies on the effects of air versus liquid in a loss of resistance technique for identification of the epidural space. The quality of the studies was evaluated by the method recommended by Cochrane Collaboration. Meta-analysis was conducted using the Cochrane Collaboration's RevMan 5.0.1 software. Results Seven prospective randomized controlled trials involving 5183 patients were included in our meta-analysis. The patients were randomly allocated into 2 groups according to the technique by which the epidural space was identified: loss of resistance with air group (group air, n = 2534) and loss of resistance with liquid group (group lidocaine, n = 2649). The results showed that the incidences of difficult catheterization, intravascular catheter insertion, incomplete block and post-operative headache were significantly lower in group liquid than in group air (P ＜ 0.05). There were no significant differences in the incidences of transient abnormal sense and accidental dural puncture between group liquid and air. Conclusion Loss of resistance with liquid can identify the epidural space more correctly than with air, with fewer complications and better analgesic efficacy.

Objective To determine the risk factors for the development of systemic inflammatory response syndrome (SIRS) in the patients after cardiopulmonary bypass has been constructed. Methods Eighty three NYHAⅠor Ⅱ patients, aged 6month-66yr, weighting 7～97 kg, undergoing cardiopulmonary bypass, were enrolled in this study. SIRS score was performed during 24 h after the surgery. The patients were divided into 2 groups: SIRS group(S, SIRS score≥2) and non-SIRS group(U, SIRS score＜2). The risk factors were identified by logistic regression analysis. Results The incidence of SIRS was 83.13% . Logistic analysis indicated that arterial oxygen pressure (PaO2), retention time, and postoperative heart rate were closely related with the development of SIRS in patients on ICU after cardiopulmonary bypass (OR＝0.518,4.334,3.607,P＜0.05). Conclusions The arterial oxygen pressure (PaO2), ICU stay, and heart rate after cardiopulmonary bypass has been constructed can be served as the risk factors for the development of SIRS in patients.