Objective To investigate the therapeutic effect of acupoint selection by heavenly stems on eosinophils(EOS) in guinea pigs with asthma.Methods Forty-eight guinea pigs were randomized into four groups: group A(timely electroacupuncture at the period of the day from 3 p.m.to 5 p.m.),group B(un-timely electroacupuncture at the period of the day from 9 a.m.to 11 a.m.),group C(model group) and group D(normal control group).Asthma was induced by chicken ovalbumin in groups A,B and C.The hematoxylin-eosin(HE) staining method and terminal-deoxynucleotidyl transferase-mediated nick end labeling(TUNEL) method were applied in the pathological and quantitative observation of eosinophils(EOS) in lung slices.Results The number of EOS was increased in model group(P

A purified polysaccharide with a galactose backbone(SPR-1,Mw 3,622 Da)was isolated from processed Polygonati Rhizoma with black beans(PRWB)and characterized its chemical properties.The backbone of SPR-1 consisted of[(4)-β-D-Galp-(1]9→ 4,6)-β-D-Galp-(1 → 4)-α-D-GalpA-(1 → 4)-α-D-GalpA-(1 →4)-α-D-Glcp-(1 → 4,6)-α-D-Glcp-(1 → 4)-α/β-D-Glcp,with a branch chain of R1:β-D-Galp-(1 → 3)-β-D-Galp-(1 → connected to the →4,6)-β-D-Galp-(1 → via O-6,and a branch chain of R2:α-D-Glcp-(1 →6)-α-D-Glcp-(1 → connected to the →4,6)-α-D-Glcp-(1 → via O-6.Immunomodulatory assays showed that the SPR-1 significantly activated macrophages,and increased secretion of NO and cytokines(i.e.,IL-1β and TNF-α),as well as promoted the phagocytic activities of cells.Furthermore,isothermal titration calorimetry(ITC)analysis and molecular docking results indicated high-affinity binding between SPR-1 and MD2 with the equilibrium dissociation constant(KD)of 18.8 μM.It was suggested that SPR-1 activated the immune response through Toll-like receptor 4(TLR4)signaling and downstream responses.Our research demon-strated that the SPR-1 has a promising candidate from PRWB for the TLR4 agonist to induce immune response,and also provided an easily accessible way that can be used for PR deep processing.

[摘 要] 目的：探讨circ_0000615在胆管癌细胞中的表达及其对胆管癌细胞增殖、迁移和侵袭能力的影响和可能的调控机制。方法：通过qPCR检测circ_0000615在正常胆管上皮HIBEC细胞和胆管癌CCLP-1、QBC939、TFK-1和RBE细胞中的表达水平。双荧光素酶报告基因实验验证circ_0000615与miR-432-5p的靶向关系。将si-NC、si-circ_0000615（si-circ-1、si-circ-2）、inhibitor-NC和inhibitor-miR-432-5p转染至CCLP-1和QBC939细胞，转染细胞分为si-NC组、si-circ-1组、si-circ-2组、si-NC+inh-NC组、si-NC+inh-miR-432-5p组和si-circ-1+inh-miR-432-5p组，通过CCK-8、EdU和Transwell实验检测各转染组胆管癌细胞的增殖、迁移和侵袭能力。结果：在胆管癌细胞中circ_0000615呈高表达而miR-432-5p呈低表达（P<0.05或P<0.01）；双荧光素酶报告基因实验证实circ_0000615与miR-432-5p之间存在靶向关系（P<0.01）；敲减circ_0000615可明显抑制CCLP-1、QBC939细胞的增殖、侵袭和迁移能力（P<0.05或P<0.01）；下调miR-432-5p可部分逆转敲减circ_0000615对胆管癌CCLP-1和QBC939细胞增殖、迁移和侵袭的抑制作用（P<0.05或P<0.01）。结论：circ_0000615通过靶向miR-432-5p调控胆管癌细胞的增殖、侵袭和迁移。