1.Regulation of Human Beta-Defensin 3(hBD-3) in Human Keratinocyte(HaCaT) Cell Lines.
Yu Jin KIM ; Chang Kwun HONG ; Seong Jun SEO
Annals of Dermatology 2003;15(1):1-7
BACKGROUND: The large surfaces of the skin are often initial site of contact between microorganism and human. The skin are coated with epidermis and epithelial cells can recognize microorganism and mount a fast defense through the production of various inducible antibiotic peptides. This leads to chracteristic broad spectrum of antimicrobial activity against bacteria, fungi, and viruses. Recent studies introduce us new peptides with antimicrobial activity such as P,-defensins and cathelicidins. They are expressed on the epithelia and polymorphonuclear leukocytes, which are first lines of defence from various invasive environments. Futhermore, they are considered very interesting and important endogenous antibiotics. Our previous study has shown that the expression of human defensin(hBD-2) mRNA, which is potent antibiotic peptide against Gram-negative bacteria(P. aeruginosa), was upregulated with ultraviolet(UV) irradiation, tumor necrosis factor-α(TNF-α) and lipopolysaccharide(LPS) in HaCaT cells. A novel hBD-3, 5-kDa, nonhemolytic antimicrobial peptide, was demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes in especially, multiresistant S. aureus. We have analyzed the expression patterns of hBD-3 in HaCaT cell lines. OBJECTIVE: This research have done in order to evaluate the expression and regulation of hBD-3 mRNA in human keratinocyte cell lines. METHODS: HaCaT cell lines were used to all culture experiments. Cultured human keratinocytes were stimulated with UV irradiation or TNF-α or LPS to determine whether hBD-3 mRNA production occurred. Reverse transcription-polymerase chain reaction (RT-PCR) was per-formed to amplify hBD-3 cDNA from stimulated keratinocytes in a time dependant manner, and densitometry was used to verify the specificity of RT-PCR amplication products. RESULTS: Expression of hBD-3 was upregulated with UV irradiation, TNF-α and LPS in Ha-CaT cells compared to control CONCLUSIONS: Human keratinocytes are capable to induce hBD-3 mRNA, as well as hBD-2, in response to UV irradiation, TNF-α and LPS. suggesting that these cells could play an important role against the bacterial infection and UV light damage in human skin.
Anti-Bacterial Agents
;
Bacteria
;
Bacterial Infections
;
Cathelicidins
;
Cell Line*
;
Densitometry
;
DNA, Complementary
;
Epidermis
;
Epithelial Cells
;
Fungi
;
Humans*
;
Keratinocytes
;
Necrosis
;
Neutrophils
;
Peptides
;
RNA, Messenger
;
Sensitivity and Specificity
;
Skin
;
Ultraviolet Rays
2.A Case of CHAP Syndrome.
Man Wook SEO ; Ji Sung KIM ; Seong Yong YU ; Byeung Soo SHIN ; Young Hyun KIM
Journal of the Korean Geriatrics Society 2001;5(4):348-353
CHAP syndorme consists of choreoathetosis, orofacial dyskinesia, affective change, hypotonia, and pseudobulbar signs. CHAP syndrome was firstly des- cribed with onset 1 to 7 days after induced profound hypothermia and complete circulatory arrest for con- genital heart lesion repair. Since then, only a few cases have been reported until now. Case We report a patient who demonstrated all of the elements of the CHAP syndrome in severe cachexic state. Brain SPECT(99mTC-ECD) shows decreased perfusion in left fronto-parieto-temporal lobe. Comments The pathogenesis of CHAP syndrome is obscure. However our case led us to suggest that more essential component pre- disposing to the CHAP syndrome is hypoxia rather than hypothermia. Furthermore, it seems to be related with circulatory insufficiency in extrapyramidal nervous systems.
Anoxia
;
Brain
;
Heart
;
Humans
;
Hypothermia
;
Movement Disorders
;
Muscle Hypotonia
;
Nervous System
;
Perfusion
3.Ovarian mature cystic teratoma with histologic features of chronic thyroiditis: Histologic analysis of 4 cases.
Doo Hyun CHUNG ; Weon Seo PARK ; Soo Min KANG ; Eun Sil YU ; Seong Hoe PARK
Korean Journal of Pathology 1992;26(3):209-214
We have reviewed ovarian mature cystic teratomas with features of chronic thyroiditis that were diagnosed at the department of pathology, Seoul National University Hospital during 7 years, 1984-1991. Twenty three case(8%) containing thyroid tissue among 285 ovarian mature teratomas were available for histopathologic examination. Among these, 4 cases(1.4%) showed lymphocytic infiltration with lymphoid follicle formation. These four cases were examined according to the strict histologic criteria of autoimmune thyroiditis and reactive non-autoimmune thyroiditis for the purpose of differentiation of pathogenesis. Two cases were similar to autoimmune in nature and the other two cases simply seemed to reflect reactive features to adjacent stimulating elements. In conclusion, thyroid tissue present in ovarian cystic teratoma may have histologic features of chronic thyroiditis of either autoimmune or non-autoimmune origin.
4.A clinical study of dupuytren contracture.
Moon Sun PARK ; Yu Seong SEO ; Soo Kyoon RAH ; Chang Uk CHOI
The Journal of the Korean Orthopaedic Association 1991;26(1):239-246
No abstract available.
Dupuytren Contracture*
5.A Case of Primary Cutaneous Cryptococcosis Misdiagnosel as Skin Tuberculosis.
Yu Jin KIM ; Seong Jun SEO ; Byung In RO
Korean Journal of Medical Mycology 2003;8(1):16-20
Cryptococcosis is an opportunistic yeast infection caused by Cryptococcosis neoformans. The cutaneous manifestations are usually polymorphic and nonspecific. Lesions may appear as subcutaneous swelling, abscesses, tumor-like masses, papules or large ulcers. Therefore, diagnosis of cutaneous cryptococcosis is quite difficult and often delayed. Clinical suspicion is very important in diagnosis. A 47-year-old man presented with a ulcerative nodule on the right side of the neck. Because initial skin biopsy revealed granulomatous lesion, we misdiagnosed his lesion as skin tuberculosis and treated him with anti tuberculosis medication for 6 months. But his symptom was not improved. After second biopsy specimen had showed some spores with narrow neck budding, we confirmed this case as a cryptococcosis and successfully treated with itraconazole (200 mg/day) for 12 weeks.
Abscess
;
Biopsy
;
Cryptococcosis*
;
Diagnosis
;
Humans
;
Itraconazole
;
Middle Aged
;
Neck
;
Skin*
;
Spores
;
Tuberculosis
;
Tuberculosis, Cutaneous*
;
Ulcer
;
Yeasts
6.A Case of Edematous Striae Distensae by Corticosteroid and Generalized Edema in Nephrotic Syndrome.
Seong Wook CHOE ; Yang Hyun YOON ; Seong Jun SEO ; Chang Kwun HONG ; Suk Hee YU
Korean Journal of Dermatology 2004;42(9):1238-1240
Striae distensae are characterized by linear, smooth bands of atrophic-appearing skin. In histologic findings, the epidermis is thin and flattened. There is a decrease in the thickness of the dermis. Separation of collagen fibers and small, fragmented elastic fibers are seen in the dermis. Striae distensae develop as a result of disruption of the connective tissue framework. Main mechanism of edematous striae distensae is disintegration of collagen, followed by the pressure induced by generalized edema as the secondary mechanism. The patient is a 17-year-old boy who had generalized pitting edema and abdominal distension due to nephrotic syndrome. On the abdomen, he has presented with edematous striae and ulceration with exudate for 1 week. Edematous striae distensae are uncommon but can develop from the combined effects of glucocorticoid and generalized edema. We report a case of edematous striae distensae in nephrotic syndrome.
Abdomen
;
Adolescent
;
Collagen
;
Connective Tissue
;
Dermis
;
Edema*
;
Elastic Tissue
;
Epidermis
;
Exudates and Transudates
;
Humans
;
Male
;
Nephrotic Syndrome*
;
Skin
;
Striae Distensae*
;
Ulcer
7.The Effect of Intraoperative Exophthalmometric Values on Enophthalmos Correction in Inferior Orbital Wall Reconstruction.
Yu Jin CHOI ; Ji Hye KIM ; Hyoun Do HUH ; Seong Jae KIM ; Seong Wook SEO
Journal of the Korean Ophthalmological Society 2017;58(7):769-775
PURPOSE: To measure the enophthalmos corrective effect after inferior orbital wall reconstruction, we compared preoperative and intraoperative exophthalmometric values with postoperative exophthalmometric values. METHODS: From January 2014 to April 2016, 60 eyes of 60 patients who underwent surgery for inferior orbital wall fracture were included. In Group 1, the exophthalmometric value was measured before surgery, during the operation, and 6 months after surgery using the Naugle exophthalmometer. In Group 2, the value was measured before surgery and 6 months after surgery using the Hertel exophthalmometer. The thickness of implants was determined by preoperative exophthalmometric values and overcorrection of 0.5 mm was performed in Group 1 patients with relatively large fractures. RESULTS: The mean age of the patients was 32.4 years in Group 1 and 34.3 years in Group 2. The mean duration between injury and surgery was 4.2 weeks in Group 1 and 2.3 weeks in Group 2. There was no statistically significant difference between preoperative exophthalmometric values in Group 1 (−1.78 ± 0.31 mm) and Group 2 (−1.81 ± 0.26 mm), but postoperative exophthalmometric values between Group 1 (−0.25 ± 0.78 mm) and Group 2 (−0.53 ± 0.46 mm) were statistically different (p = 0.034). CONCLUSIONS: The exophthalmometric values and wall fracture size are important factors for determining implant thickness of inferior orbital wall reconstruction. Intraoperative measurement of exophthalmometric values should be considered in inferior orbital wall reconstruction for enophthalmos correction.
Enophthalmos*
;
Humans
;
Orbit*
8.A case of Verrucous Carcinoma Arising on the Glans Penis with Insertion of Penile Prosthesis.
Yu Jin KIM ; Seong Wook CHOI ; Seong Jun SEO ; Kye Young SONG ; Chang Kwun HONG
Korean Journal of Dermatology 2003;41(3):347-350
Verrucous carcinoma is a variant of well-differentiated squarmous cell carcinoma. Although this tumor is well-documented in the oral cavity, its occurrence on the glans penis is rare. It is a relatively nonaggressive tumor which seldom metastasizes to the resional lymph node or distantly. With proper treatment, the prognosis is excellent. We have experienced a case of verrucous carcinoma arising on the glans penis with the previous insertion of paraffin and penile prosthesis for the augumentation of penis, which was treated with total phallectomy. We report this case with a review of literature.
Carcinoma, Verrucous*
;
Lymph Nodes
;
Male
;
Mouth
;
Paraffin
;
Penile Prosthesis*
;
Penis*
;
Prognosis
9.A Case of Malignant Proliferating Trichilemmal Tumor.
Yu Jin KIM ; Seong Jun SEO ; Kye Yong SONG ; Chang Kwun HONG
Korean Journal of Dermatology 2003;41(3):391-393
Malignant proliferating trichilemmal tumor is a rare neoplasm derived from the outer root sheath of the hair follicle. We report a 64-year-old woman with nodular mass in her scalp of for 10 years. Despite surgical removal of the mass, the residual lesion grew with ulceration. In fine needle aspiration, malignant cells were detected. Histological examination of the skin lesion revealed a lobulated tumor exhibiting keratinization beneath the epidermis. In some area, cord of atypical cells infiltrating surrounding tissue, stromal desmoplasia, mitotic figures were seen. For treatment wide surgical excision and skin graft were done.
Biopsy, Fine-Needle
;
Epidermis
;
Female
;
Hair Follicle
;
Humans
;
Middle Aged
;
Scalp
;
Skin
;
Transplants
;
Ulcer
10.Treatment and retention of relapsed anterior open-bite with low tongue posture and tongue-tie: A 10-year follow-up.
Yu Jin SEO ; Su Jung KIM ; Janchivdorj MUNKHSHUR ; Kyu Rhim CHUNG ; Peter NGAN ; Seong Hun KIM
The Korean Journal of Orthodontics 2014;44(4):203-216
The purpose of the current report is to present 6-year long-term stability and 10-year follow-up data for an adult patient who was treated with a tongue elevator for relapsed anterior open-bite. The 19-year-old male patient presented with the chief complaint of difficulty in chewing his food. Collectively, clinical and radiographic examinations revealed an anterior open-bite, low tongue posture, and tongue-tie. The patient opted for orthodontic treatment alone, without any surgical procedure. A lingual frenectomy was recommended to avoid the risk of relapse, but the patient declined because he was not experiencing tongue discomfort. Initial treatment of the anterior open-bite with molar intrusion and tongue exercises was successful, but relapse occurred during the retention period. A tongue elevator was used for retreatment, because the approach was minimally invasive and suited the patient's requirements regarding discomfort, cost, and time. The appliance changed the tongue posture and generated an altered tongue force, which ultimately resulted in intrusive dentoalveolar effects, and a subsequent counterclockwise rotation of the mandible. The results showed long-term stability and were maintained for six years through continual use of the tongue elevator. The results of this case indicated that a tongue elevator could be used not only as an alternative treatment for open-bite, but also as an active retainer.
Adult
;
Elevators and Escalators
;
Exercise
;
Follow-Up Studies*
;
Humans
;
Male
;
Mandible
;
Mastication
;
Molar
;
Posture*
;
Recurrence
;
Retreatment
;
Tongue*
;
Young Adult