Depression ; classification ; diagnosis ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

Objective To report the technique and outcome of combined transplantation of bilateral vascularized fibulas to repair the segmental femoral defect Method In the period from October 1984 to May 1992, bilateral vascularized fibula transfer was done to treat femoral defects in 9 cases, in which there were 5 cases of bone tumor, 3 cases of pathological fractures following chronic osteomyelitis and 1 case of posttraumatic bone defect The length of the transferred fibulas ranged from 12 cm to 22 cm and averaged 16.25 cm Result Except 1 case in which thigh amputation was done 6 months after operation because of local recurrence of the chondrosarcoma, all the transferred fibulas united solidly with the host bones 3-6 months after operation The repaired limbs started weight-bearing at the third to seventh postoperative month Conclusion To repair a femoral defect over 10 cm in length, combined transfer of bilateral vascularized fibulas is the treatment of choice with short therapeutic process and good results.

Hemodynamic effects of Laigechongji (LGCJ)were studied in anesthetized open-chest dogs. LGCJ(in doseof 8. 5 and 17.0g/kg,id. )decreased significantly SBP, DBP, MAP, HR, LVSP, +dp/dt max and TPVR, 30~45 mins after medication,and continued for about 90~ 150mins. However,they had no significant effects ondp/dt max,LVEDP,CO,CI,SV and SI. These results suggest that LGCJ may cause hypotension,and its hypotensive effects may be related to its bradycardiac,negative inotropic and vascular resistance reducing activities.

Objective To investigate the influence of tooth preparation surface roughness and different dental ce-ments on adhesive strength, and provide some reference information on the selection of dental rotary instruments and dental cements. Methods Sixty dentin samples were prepared and randomly divided into 3 groups. Samples from group A, B and C were grinded by diamond burs with grit-sizes of 125μm, 60μm and 28μm respectively. Two samples selected randomly from each group were observed under scanning electron microscope (SEM) for the surface microprofile. The remaining 18 samples from each group were evenly divided into 3 subgroups. Each subgroup was teamed with one of 3 dental cements:zinc polycarboxylate cement (ZP), glass ionomer cement (GI) and resin-modified glass ionomer cement (RMGI). Co-Cr alloy casts were cemented onto the dentin samples, and the adhesive strength was tested. Meanwhile, the types of failure were re-corded for each sample. Results The surface roughness of tooth preparation samples showed a downward trend in group A, group B and group C. Among the involved 3 dental cements, RMGI exhibited the highest adhesive strength, and there was no significant difference in the adhesive strength between ZP and GI. Among different combination subgroups, A-RMGI had the highest adhesive strength, B-RMGI run the second place, while C-ZP and C-GI were proved the lowest. However, there was no interaction between the two factors. Furthermore, group A mainly showed failure typesⅠandⅡ, group B evenly showed all the failure types and group C mainly showed failure typesⅡandⅢ. Conclusion The adhesive strength is related to the surface roughness. RMGI exhibits the highest adhesive efficiency. There is no obvious compatibility in particular surface roughness and dental cements.

Objective:To observe the effects of real-time and retrospective analyze of cardiac remote monitoring based on differential threshold method.Methods: Xin An Bao XAB - M3AG ECG remote monitoring system which based on the differential threshold method of improvement being used to determine the parameters of electro cardio signal was installed in 8957 patients.Results: We had collected all 46967 ECG real-time data, including 9564 manual transmission, 25830 timing transmitting and 11573 automatically transmission. 5728 of automatically transmission was with arrhythmia (49.5%) and the others of automatically transmission was attribute to inference.Conclusion: The results showed that algorithm complexity based on the improved differential threshold method is low, the precision is high and it has a good real-time performance. It realized the real-time monitoring of ECG.

The effect of Hippophae rhamnoides L. juice (HRJ) on the immunologic function and the inhibition of tumor growth in mice were reported. Kunming mice were administered orally with HRJ for 7 days. The IL-2 produced by splenocytes, the reactivity of splenocytes to IL-2 were markedly stimulated (P

Objective To investigate the content changes of two alkaloid before and after the processing of Aconitumvilmorinianum and its effect on cardiotoxicity. MethodsThe UPLC method was used to determine the content of yunaconitine and 8-acetylacetonarine before and after the processing of A.vilmorinianum. The half lethal dose (LD50) of A.vilmorinianum to rats was determined by Bliss method. Rats wereig administrated A.vilmoriniauum and its processed products for 14 d. The chages of II lead electrocardiogram of anesthetized rats and myocardial morphology were observed,and the serum levels of lactate kinase (CK) and lactate dehydrogenase (LDH)were detected. Results The content of yunaconitine before and after the preparation of A.vilmorinianum was 263.96 μg/g and not detected respectively. The content of 8-acetylacetonarine was not detected and 568.47 μg/g respectively. The LD50 of the raw product was crude drug4.2 g/kg, and the maximum dose of the processed product was crude drug18.0 g/kg; In the anesthetized rats of the raw group, ventricular premature beats, ventricular bipolar law, and ventricular fibrillation occurred successively. The processed product group showed bradycardia in the early stage and returned to normal in 60 min; Compared with the blank group, the LDH and CK levels in the raw group were significantly increased (P< 0.05), and only the LDH level was significantly increased in the processed product group (P< 0.05); The gap between myocardial fibers in the raw group was significantly enlarged, and the infiltration of inflammatory cells was obvious. The myocardial fibers of the product group are arranged neatly, and individual inflammatory cells infiltrated in the interstitial. Conclusion At the given dose, A.vilmorinianum has greater cardiotoxicity, and the toxicity of A.vilmorinianum is obviously reduced after processing.

OBJECTIVE: Todevelop a rapid and sensitive UHPLC-Q-orbitrap method for the identification of chemical constituents in Nelumbinis Folium. METHODS: Thermo Scientific Hypersil GOLD aQ(2.1 mm×100 mm,1.9 μm) was used with 0.1% formic acid-acetonitrile solution and 0.1% formic acid-water solution as the mobile phase in gradient elution. The extract was detected by Q-orbitrapmass spectrometry equipped with an ESI ion source in both positiveand negative mode. RESULTS: Based on the accurate mass-to-charge measurements, retention time, mass fragmentation patterns, compound discoverer software which can link to local compound database of Traditional Chinese Medicine from Thermo Scientific and online compound database from mzCloud, using Mass Frontier software to explain the fragmentations, a total of 53 compounds were screened and characterized, including 21 flavonoids,5 terpenes,13 alkaloids, 3 coumarins, 3 amino acids, 2 lignans and 6 other compounds. CONCLUSION: These results are helpful for the comprehensive quality control, better comprehension of the metabolism, and further study of the pharmacodynamic substances from Nelumbinis Folium.

OBJECTIVE: To prepare starch microspheres of diclofenac sodium by inverse crosslink emulsification method. METHODS: Mean particle size and encapsulation efficiency of starch microspheres were used as indicators, single factor and orthogonal design methods were performed for optimizing preparation process and formulations of starch microspheres. The appearance and structure of the microspheres were researched by optical microscope, transmission electron microscope, infrared spectroscopy and differential thermal analyzer. In vitro drug release behavior was investigated by dialysis method. RESULTS The preparation conditions optimized by orthogonal design; the concentration of starch 10%, crosslinking temperature 55℃, dosage of cross-linking agent 0.2 g, the volume ratio of oil phase and water 5:1, amount of emulsifier 5 mL, cross-linking time 60 min. Starch microsphere obtained by above condition has an average particle size of 9 μm, appearance is irregular spherical shape, and encapsulation efficiency was 67.52%. Results of IR spectra and DTA curves showed that starch cross-links have happened. CONCLUSION In vitro releasing curve showed that the diclofenac sodium starch microspheres have good sustain release effect, drug release complies with the Weibull equation.

OBJECTIVE: To prepare coenzyme Q10-γ-cyclodextrin nano-crystal suspension using high pressure homogeneous combining freeze-drying method, optimize the preparation technology, and evaluate the quality of the product. METHODS: Encapsulation efficiency and mean particle size of inclusion complex were used as indicators, and response surface method was performed for optimizing preparation process and formulations. The stability and transdermal performance in vitro of the inclusion complexes were investigated. RESULTS: The optimal technical parameters were as follows; the mass ratio of γ-cyclodextrin and drug, 7.76:1; ultrasonic temperature, 40℃; ultrasonic time, 28.33 min; homogeneous pressure, 40 MPa. Under such conditons, an average encapsulation efficiency of 48.39% and average particle size of 324 nm were obtained for the nano-crystal suspension. The transdermal experiment in vitro showed that the infiltration rate of inclusion was 5.68 μg·cm-2·h-1. CONCLUSION: The preparation method is simple and feasible. The product quality is stable. Moreover, it is easy to manufacture.