Animals ; Ascorbic Acid ; metabolism ; Brain Ischemia ; metabolism ; Glutamic Acid ; metabolism ; Hippocampus ; drug effects ; metabolism ; Male ; Propofol ; pharmacology ; Rats ; Rats, Wistar ; Reperfusion Injury ; metabolism

The linear regression method of multiwavelength data and orthogonal functions spectropho-tomtry have been used for the determination of sulfamethoxazole and triemethoprim in co-trimo-xazole tablets without any preliminary separation.The methods are simple and rapid.Good results have been obtained. The average recovery of sulfamethoxazole was 99.72-100.5%, with coefficient of variation below 0.48%, and the average recovery of triemethoprim was 99.26-99.46%, with coefficient of variation below 0.83%. The accuracy and precision meet the requirements of analysis of the pharmaceutical preparations

〔Abstract〕 The paper introduces the connotation of digital learning environment and digital scientific research environment, analyzes the strategies of constructing digital learning environment and digital scientific research environment in hospitals, including hospital librar-ians taking part in scientific research knowledge service, doctors, teachers and researchers participation, creating personalized scientific research information environment, etc.

Animals ; Female ; Hypertension, Pregnancy-Induced ; metabolism ; pathology ; Kidney ; metabolism ; pathology ; Pregnancy ; Protein Kinase C-alpha ; genetics ; metabolism ; Rats ; Rats, Wistar ; Transcription, Genetic

In contrast to the decreasing prevalence of gastric cancer and esophageal cancer,there has been an alarm rise in the incidence and prevalence of adenocarcinoma of esophagogastric junction during recent literatures.Many discrepancies exists in the current literature,however,regarding the etiology,classification and surgical treatment of the tumor.This confusion is due to a lack of clear current UICC recommendation for the classification and staging.Consequently,the selection of the surgical procedure for tumor is controversial.A clear definition and classification is,therefore,the prerequisite for a discussion of the optimal surgical approach.This review give a detailed description of the related concept and recent advances in treatment of adenocarcinoma of the esophagogastric junction.

Animals ; Astrocytes ; metabolism ; Brain Ischemia ; metabolism ; pathology ; Glial Fibrillary Acidic Protein ; metabolism ; Neuroprotective Agents ; pharmacology ; Propofol ; pharmacology ; Random Allocation ; Rats ; Rats, Wistar ; Reperfusion Injury ; metabolism ; prevention & control

Objective To discuss the effect and related mechanism on epithelial?mesenchymal transition(EMT)process and invasive ability of the trophoblasts JEG?3 by targeted silencing of Notch?1 gene. Methods Notch?1?siRNA interference fragment was designed and synthesized to sta?bly transfect JEG?3 cells. Changes in the invasive ability of trophoblasts were detected by Transwell chamber invasion assay. The expressions of Notch?1 ,E?cadherin ,vimentin and EMT transcriptive regulators slug ,snail ,twist were analyzed in transfected trophoblasts by Western blot. Results The relative expression of Notch?1 protein in JEG?3 and the invasive ability of JEG?3 were significantly decreased after transfection with Notch?1?siRNA,the expression of the epithelial indictor E?cadherin was significantly increased while the mesenchymal indicator vimentin was de?creased with the reduction of EMT transcriptive regulators slug,snail and twist. Conclusion The invasion of trophoblasts was affected by targeted si?lencing of Notch?1 gene and Notch?1 can reduce the invasion of trophoblasts through influencing EMT process.

Objective To investigate the relationship among the activity of protein kinase C (PKC) in platelets of maternal vein and umbilical blood , the pathophysiological changes of pregnancy induced hypertension (PIH) and fetal growth restriction (FGR) in PIH patients. Methods Activities of PKC in membrane and plasma of platelets from maternal vein and umbilical blood taken from 35 PIH patients and 20 normal pregnant women were measured with substrate phosphorylation method. Results No difference was shown in the PKC activities between the mild PIH patients and normal pregnant women in both maternal and cord blood.The PKC activities in moderate and severe PIH patients were significantly higher than those of the normal pregnant group.In normal pregnant women, the PKC activity in membrane and plasm of the platelets had no significant difference. In the moderate and severe PIH group, PKC activity in membrane was far more higher than the plasm 46?6 vs 37?4 pmol/(min?mg protein), P

The binding characteristics of quinolone antibacterial agent, ofloxacin (OFLX) and ciprofloxacin(CPFX) with catalase(CAT) were studied by fluorescence spectroscopy in aqueous solution. It was showed that both OFLX and CPFX had a strong ability to quench the CAT intrinsic fluorescence through a static quenching procedure. The formation constant K and the number of bind ing site n were further calculated according to the fluorescence quenching r esults.

0 05)before and after 1 year in patients with and without HAV superinfection. The amount of HBV DNA and HBeAg positive rate in serum from HAV infection recovery stage of patients with CHB were higher than those in acute stage( P