This study was designed to evaluate the influence of fibroblasts or connective tissue from mouse oral mucosa on differentiation of neonatal mouse calvaria-derived osteoblasts and mineralization of bone nodules. Primary cell cultures from mouse calvarial osteoblasts and 2-4 passaged fibroblasts from oral mucosa were co-cultured in monolayer cultures, devided into 6 experimental group according to cell density or cell confluency. Osteoblasts were also co-cultured with fibroblasts in Transwell(R) culture plate with different co-cultured period according to osteoblast differentiation. The alkaline phosphatase activity were measured in monolayer cultures and cultures using Transwell(R). The mineralized bone nodules were presented by Von Kossa staining and density of mineralized nodules was measured by image analysis. The connective tissues with or without osteoblast seeding were cultured and examined histologically by Von Kossa and Trichrome Goldner staining. The results were as follows; 1. Prolonged maturation of matrix and delayed mineralization of bone nodules were resulted in monolayer cultures. 2. Co-culture of fibroblast with osteoblast using Transwell(R) during osteoblast proliferation stage stimulated proliferation of osteoblasts and increased alkaline phosphatase activity and mineralization of bone nodules. 3. Co-culture of fibroblast with osteoblast using Transwell(R) during matrix mineralization stage decreased and delayed mineralization of bone nodules. 4. In vitro cultured connective tissue with osteoblast seeding resulted in proliferation of osteoblasts and matrix formation with mineralization.
Alkaline Phosphatase ; Animals ; Cell Count ; Coculture Techniques ; Connective Tissue ; Fibroblasts* ; Mice* ; Mouth Mucosa ; Osteoblasts ; Primary Cell Culture

No abstract available.
Graft Survival* ; Humans ; Living Donors* ; Risk Factors* ; Transplants*

No abstract available.
Cause of Death* ; Kidney Transplantation* ; Kidney*

No abstract available.
Humans ; Kidney Transplantation*

No abstract available.
Humans ; Kidney Transplantation*

PURPOSE: To report a case of laser photocoagulation treatment for the patient of Coats' disease accompanying a retinal macrocyst. CASE SUMMARY: A three-year-old boy visited the hospital whose chief complaint was visual acuity decrease of his left eye. Fundus examination showed macular scar, foveal hard exudates and inferior retinal cystic lesion in his left eye. Two months later, examination under anesthesia (EUA) and fluorescein angiography (FAG) was performed. The results revealed inferior retinal macrocyst, nasal avascular retina and telangiectasia around the retinal macrocyst. Laser photocoagulation was performed around the retinal macrocyst and at the nasal avascular retina. One year after the laser photocoagulation, retinal macrocyst did not further progress and the retina was stabilized. CONCLUSIONS: Laser photocoagulation was done around the retinal macrocyst and at the nasal avascular retina of the Coats' disease accompanying a retinal macrocyst and the lesions did not further progress and the retina was stabilized.
Anesthesia ; Cicatrix ; Exudates and Transudates ; Eye ; Fluorescein Angiography ; Humans ; Light Coagulation ; Retina ; Retinaldehyde ; Telangiectasis ; Visual Acuity

No abstract available.
Child* ; Humans ; Kidney Transplantation*

No abstract available.
Allografts* ; Humans ; Ultrasonography*

No abstract available.
Cause of Death* ; Kidney Transplantation* ; Kidney*

No abstract available.
Child* ; Humans ; Kidney Transplantation*