Amniotic Fluid ; cytology ; Cardiomyopathies ; therapy ; Humans ; Stem Cells ; cytology

Objective To analyze the clinical characteristics of children infecting with Epstein -Barr virus. And in purpose to improve the knowledge of children infecting with Epstein -Barr virus.Methods We analyzed retrospectively to 97 cases of children which serodiagnosis of Epstein -Barr virus infectius in the aspects of the onset season,the age,clinical characteristics.And compared with complication group and no complication group in the aspects of the white cell count,neutrophils to white cell count ratio,lymphocyte to white cell count ratio and C -reactive protein.And contrasted the presence of statistical difference.Results The peak months were spring and summer(58 cases);Pre -school age was the peak age of EBV infectious(41 cases),and the rest respectively were toddlers age(33 cases),school age and youth(20 cases)and infant period(3 cases).The most frequently clinical manifestation respectively was pharyngitis,and other such as fever,lymphadenopathy,hepatosplenomegaly.There had no statistical difference in the two groups of complication group and no complication group in the aspects of the white cell count,neutrophils to white cell count ratio,lymphocyte to white cell count ratio and C -reactive protein(P >0.05).Conclusion The infant can suffer from infectious mononucleosis infect with EBV.Infectious mononucleosis have a variety of clinical manifestation,which always have some complications.The diognosis should combinate clini-cal manifestation with laberatory test.

Though parched Chinese herbal medicines contain less effective or index components, their pharmacological actions do not reduce or even become improved to some extent. However, the current studies related to material basis could not explain the changes in property, flavour and efficacy of parched Chinese herbal medicines. Meanwhile, due to the lack of objective and specific evaluation indexes, the quality evaluation could not reflect features of parched Chinese herbal pieces. Therefore, how to break the bottleneck for the studies on parched Chinese herbal pieces, make further innovation and conduct in-depth studies on the material basis of parched Chinese herbal medicines are common problems that medical scholars are facing. According to the findings in the previous studies, the author proposed to explain the material basis of parched Chinese herbal medicines by studying Maillard reaction and establish specific quality evaluation indexes according to the features of parched Chinese herbal pieces, and conducted relevant studies.
Drug Compounding ; methods ; Drugs, Chinese Herbal ; chemistry ; Maillard Reaction ; Quality Control

As an indispensable part of minority traditional medicine, mineral medicine has used with less dosage and reliable efficacy for the last thousand years. Based on the unearthed relics and medical literatures of past dynasties, the history of Han nationality ap- years, which had been recorded in main literature. But there is less comprehensive report of its usage in the other 55-minority nationality. This article was based on the analysis of ethnic minority literature of thousands of years, and conducted a comprehensive collation and analysis of mineral medicine. It was mostly determined that there was 20 minority groups using mineral medicine, with a total of 163 species (limited our references), and the most used is the Tibetan, accounting for 141. The most serious problems of mineral medicine are that species should be further investigated and researched, and then become the legal commercial medicine, and the classification principles of mineral medicine should be established. Through the traditional processing and experimental studies, the problems of attenuation and detoxification should be solved.
China ; ethnology ; History, Ancient ; History, Medieval ; Humans ; Medicine in Literature ; Medicine, Chinese Traditional ; history ; Minerals ; analysis ; pharmacology

· Application of capsular tension ring ( CTR ) in phacoemulsification has become a common method to increase the stability of the capsular bag. CTR can effectively reduce the posterior capsular opacification ( PCO) , prevent intraocular lens ( lOL ) decentration and tilt, not cause lOL degree deviation and aberration increase.ln this review, we summarized the development overview of CTR in phacoemulsification.

AIM: To explore the effect of lacrimal duct laser with lacrimal drainage tubes and stents implantation for complexity dacryagogatresia.
METHODS: There were 65 patients ( 82 eyes ) with compound tears nasolacrimal duct obstruction who received lacrimal drainage tubes and stents implantation after laser. The lacrimal duct stents were removed through nasal cavity after 1mo. Lacrimal drainage tubes were removed after 3-6mo. Follow-up periods were 6mo to 1a.
RESULTS: In the 65 patients (82 eyes), 71 eyes were cured, 5 eyes improved, the efficient rate was 93%; there were 6 eyes (7%) with epiphora.
CONCLUSION: Lacrimal duct laser with lacrimal drainage tubes and stents implantation was efficient for complexity dacryagogatresia.

Objective To study the changes of the amplitude of ion channels currents in developing hippocampal neurons.Methods Using whole-cell patch-clamp techniques in cultured hippocampal neurons whose cultured day were 6 d and 16 d,respectively,changes of the amplitude of ion channels currents in developing hippocampal neurons were explored.Results Compared with the hippocampal neuron whose cultured day was 6 d,there were no statistical differences of the amplitude of voltage dependent sodium currents of hippocampal neuron whose cultured day was 16 d.The amplitude of voltage dependent potassium currents of hippocampal neuron whose cultured day was 16 d were significantly increased(P

Objective To study the changes of N-methyl-D-aspartate(NMDA)-receptor-channels current in developing hippocampal neurones during hypoxia and effect of adenosine intervention.Methods Whole-cell patch-clamp techniques cultured hippocampal neurons whose cultured day were 6 days and 16 days respectively,the amplitude of the NMDA-receptor-channels currents of hippocampal neuron were determined.And the effect of hypoxia on the NMDA-receptor-channels current,and adenosine regulatory mechanisms in cultured hippocampal neurons were explored.Results During hypoxia,compared with control group,the amplitude of the NMDA-receptorchannels currents of hippocampal neuron whose cultured day was 6 days were significantly increased(P

Objective To establish the Flow-FISH method for simultaneous detection of telornere length and cell differentiation antigen. Methods HL60, Raji, Molt4 cells were cultivated. Each step and the conditions of the Flow-FISH procedure were optimized, standardized and validated, then 14 acute leukemia patients were observed for the changes of telomere length combined with differentiation antigen after complete remission by the method. Results Cells were stained with Alexa Fluor(R) 647-labeled antibody. Anti-gen-anfibedy complexes were covalenfly cross-linked onto the cell membrane before telomere staining. Cells were hybridized with telomere-specific fluorescein isothiocyanate (FITC)-conjugated peptide nucleic acid (PNA) probes followed by being counterstained with propidium iodide(PI). Multicolor Flow-FISH was performed to analyze telomere length and differentiation antigen simultaneously. The patients showed longer telomere and lower antigen expression after complete remission. Conclusion The Flow-FISH method for simultaneous detection of telomere length and differentiation antigen was successfully established which might prove to be a promising means for leukemia research especially in those without special molecular markers.

Objective To explore the effect of minimally invasive clinical pathway in patients after car-diac interventional therapy. Methods One hundred and twenty patients who received cardiac interventional therapy from June 2005 to October 2006 were divided into the control group and the observation group.The control group received routine nursing while the observation group adopted minimally invasive clinical pathway of nursing.The nursing effect in the two groups was compared. Results The mean hospitalized duration, sat-isfaction degree and health knowledge level in the observation group were superior to those of the control group (P ＜ 0.05). Conclusions The adoption of minimally invasive clinical pathway in patients after cardiac inter-ventional therapy could increase working efficiency nd ensure the nusing quality.