Objective] To explore professor Yu Jingmao's clinical experience in coordinating and treating pediatric disease with herbal paste. [Method] By fol owing the teacher clinic, the author summarizes professor Yu Jingmao's academic experience of treatment of children with chronic il ness with herbal paste, and for proven cases. [Result] Professor Yu Jingmao uses herbal paste for modulating pediatric intractable disease with curative effect. In his opinion, herbal paste treatment of pediatric diseases must be combined with disease and syndrome differentiation, and considered in accordance with seasonal conditions and the patient's individuality. Professor Yu considers that herbal paste formulations are suitable for children, and pays attention to regulating and nourishing to strengthen the body focused on harmony, reinforcing with elimination based on balance, protecting and tonifying the spleen and stomach based on enjoying oral application. [Conclusion] According to children's physiological and pathological characteristics, professor Yu Jingmao has rich experiences in using herbal paste for personalized syndrome differentiation treatment of pediatric diseases to strengthen body resistance and eliminate evil, regulate Yin and Yang, which is worth generalizing and applying.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods ; Neoplasms ; diagnosis ; drug therapy ; prevention & control ; Phytotherapy ; Treatment Outcome

Humans ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn ; Infant, Premature

Objective: Benzoquinone ansamycin antibiotic, geldanamycin (GA), is a new anticancer agent that could inhibit Hsp90 by occupying its NH2-terminal ATP-binding site. This study was to investigate the antitumor efficacy of GA on Her2/neu tyrosine kinase overexpressing human breast cancer cell line SKBr3. Methods: The degradation of Her2/neu tyrosine kinase was analyzed by Western blotting, the proliferation index was determined by MTT assay, cell cycle distribution was detected by flow cytometry, Cyclin D1 mRNA transcription was measured by RT-PCR and real-time PCR, and cell motility was evaluated by the cell culture insert model. Results: GA induced a dose- and a time-dependent degradation of the Her2/ neu tyrosine kinase protein and concurrently, the inhibition of cancer cell proliferation. The antitumor effects mediated by GA included: GA treatment decreased the survival rates of cancer cells, and led to a dose-dependent G1 arrest. Furthermore, this antitumor effect was proved to be related to declined transcription of Cyclin D1. Concurrently, the motility of cancer cells was reduced by GA. Conclusion: GA treatment could induce the degradation of Her2/neu tyrosine kinase efficiently, inhibit cancer cell proliferation and reduce motility in Her2/neu tyrosine kinase overexpressed human breast cancer cell line SKBr3.

Objective: Benzoquinone ansamycin antibiotic, geldanamycin (GA), is a new anticancer agent that could inhibit Hsp90 by occupying its NH2-terminal ATP-binding site. This study was to investigate the antitumor efficacy of GA on Her2/neu tyrosine kinase overexpressing human breast cancer cell line SKBr3. Methods: The degradation of Her2/neu tyrosine kinase was analyzed by Western blotting, the proliferation index was determined by MTT assay, cell cycle distribution was detected by flow cytometry, Cyclin D1 mRNA transcription was measured by RT-PCR and real-time PCR, and cell motility was evaluated by the cell culture insert model. Results: GA induced a dose- and a time-dependent degradation of the Her2/ neu tyrosine kinase protein and concurrently, the inhibition of cancer cell proliferation. The antitumor effects mediated by GA included: GA treatment decreased the survival rates of cancer cells, and led to a dose-dependent G1 arrest. Furthermore, this antitumor effect was proved to be related to declined transcription of Cyclin D1. Concurrently, the motility of cancer cells was reduced by GA. Conclusion: GA treatment could induce the degradation of Her2/neu tyrosine kinase efficiently, inhibit cancer cell proliferation and reduce motility in Her2/neu tyrosine kinase overexpressed human breast cancer cell line SKBr3.

This study was aimed to investigate the dose-response relations and synergy effects of bioactive components in Ginkgo bilobaon antiplatelet aggregation and DPPH free radical scavenging.The antiplatelet aggregation experiment and DPPH free radical scavenging experiment were conducted respectively to investigate the dose-response relations and synergy effects.Firstly,the effect of inhibiting platelet aggregation induced by PAF of ginkgolides in rabbits was investigated.And then,the effect of DPPH free radical scavenging by ginkgo flavonoids was also investigated.Finally,the synergy effects among effective components were studied.The results showed that ginkgolide A,ginkgolide B,ginkgolide C and bilobalide had dose-response relations on antiplatelet aggregation.Quercetin,kaempferol and isorhamnetin had DPPH free radical scavenging effects with dose-response relation.It was concluded that ginkgolide had the dose-response relation on antiplatelet aggregation.Ginkgolide A and ginkgolide B had synergy effect.Ginkgo flavonoids had DPPH free radical scavenging effect.Quercetin and isorhamnetin had synergy effect.

A large number of animal experiments have confirmed that ischemic preconditioning can produce a powerful organ protective effect,but the progress and results of the conversion of animal experiments to clinical trials are unsatisfactory.It has great significance for studying of molecular mechanisms of ischemic preconditioning mediated neuroprotection,searching for safe and effective preconditioning induced ways which can be converted to clinical practice,improving the tolerance of nerve tissue ischemia and hypoxia in stroke and surgical patients and achieving a safe and effective neuroprotection.This article reviews the molecular mechanisms of ischemic preconditioning mediated neuroprotection from the aspects of pretreatment of activated receptor,mitochondria,transcription factor,and protein kinase.

Textbook construction is an important part of building TCM English teaching system and its key research point is summarizing and exploring the basic principles and laws for guiding the compilation practice. This article tried to apply the theory of English for Specific Purpose and combine the compilation practice of English for TCM to explore the particular principles for compiling TCM English textbook.

Objective To investigate the changes in myeloid-derived suppressor cells (MDSC), regulatory T cells (Treg) and T helper 17 (Th17) cells in peripheral blood of patients with HPV infection and cervical diseases including ectopic cervical columnar epithelium (CE), cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CC), and to analyze the roles of MDSC, Treg and Th17 cells in the development of cervical diseases.Methods This study enrolled 120 HPV-positive patients with cervical diseases (18 cases of CE, 50 cases of CIN, 52 cases of CC), 20 HPV-negative patients with cervical diseases (10 cases of CE and 10 cases of CIN) and 20 healthy subjects from March 2011 to December 2016.Changes in MDSC, Treg and Th17 cells in peripheral blood of all patients were detected by flow cytometry.Results Percentages of MDSC, Treg and Th17 cells in peripheral blood of patients who were positive for HPV were significantly higher than those in HPV-negative patients (P<0.05).Besides, percentages of these cells gradually increased with the exacerbation of cervical disease (CE-CINⅠ-CINⅡ-CINⅢ-CC) (P<0.05).Patients who were infected with high-risk HPV had significantly higher percentages of MDSC, Treg and Th17 cells in peripheral blood than those infected with low-risk HPV (P<0.05).Changes of MDSC, Treg and Th17 cells in peripheral blood of HPV-positive patients with cervical cancer were related to International Federation of Gynecology and Obstetrics (FIGO) stage, tumor differentiation, lymph node metastasis and vascular invasion (P<0.05).MDSC, Treg and Th17 cells in peripheral blood of all patients were significantly reduced after treatment (P<0.05).Patients whose status changed from HPV-positive to HPV-negative following treatment also showed significantly decreased MDSC, Treg and Th17 cells in peripheral blood as compared with those who remained HPV-positive (P<0.05).MDSC, Treg and Th17 cells in peripheral blood of HPV-positive patients with cervical diseases were positively correlated (r=0.599, 0.677, 0.648;P<0.05).Conclusion MDSC, Treg and Th17 cells in peripheral blood of patients with HPV infection are associated with the pathological process, severity and clinical pathological features of cervical diseases as well as therapeutic effects.Therefore, they can be used as biomarkers to detect the occurrence and development of HPV-positive cervical diseases.

Trauma and infection may cause a stress reaction to the body,which leads to the metabolic imbalance of organs and tissues,but some treatment methods can effectively reduce the negative effects of undesirable stress on body metabolism.This article focuses on the effects of trauma and infection stress on the protein metabolism,furthermore,introduces some related treatment methods.