Objective To investigate the bladder responses to different rates of bladder infusion in healthy and spinal cord injured rats.Methods Fourteen adult female Sprague-Dawley rats were used and divided into two groups:healthy group (n =7) and spinal transection injury group (n =7).The intravesical pressures induced by continuous infusion of saline with different rates under general anesthesia were recorded and compared between the two groups.Results In healthy group the responses of bladder contraction were dependent upon the rate of bladder infusion,and contraction time became shorter while increasing the infusion rate (P ＜ 0.05),i.e.contraction time was (401.0±132.4),(215.7 ±95.9),(108.3 ±59.1) and (52.5 ±32.8)s,respectively,when the infusion rate was 0.05,0.1,0.2 and 0.5 mL/min.Compared with healthy group,spinal transection injury group had significantly shorter bladder contraction time (P ＜ 0.05),however the relationship between bladder contraction time and infusion rate was much weakened.Spinal transaction injury rats had significantly lower peak of bladder pressure (P ＜ 0.05) compared with healthy rats.There was no significant difference for contraction durations between the two groups.Conclusions The infusion rates influenced the contraction periods for both healthy and spinal cord injured rats.During bladder infusions with a same rate,the contraction time and the peak of bladder pressure were determined by bladder conditions,i.e.healthy or neurogenic with spinal cord injury.

Calculation of linear parameters, such as time-domain and frequency-domain analysis of heart rate variability (HRV), is a conventional method for assessment of autonomic nervous system activity. Nonlinear phenomena are certainly involved in the genesis of HRV. In a seemingly random signal the Poincaré plot can easily demonstrate whether there is an underlying determinism in the signal. Linear and nonlinear analysis methods were applied in the computer words inputting experiments in this study for physiological measurement. This study therefore demonstrated that Poincaré plot was a simple but powerful graphical tool to describe the dynamics of a system.
Autonomic Nervous System ; physiology ; Heart Rate ; Humans ; Linear Models ; Nonlinear Dynamics

Child ; Humans ; Karyotyping ; Male ; Osteochondrodysplasias ; classification ; diagnosis ; genetics

Child, Preschool ; Dwarfism ; congenital ; diagnosis ; Humans ; Male ; Osteochondrodysplasias ; congenital

10.3969/j.issn.2095-4344.2013.24.007

Data were analyzed from self-filled questionnaires for the students who took the elective course of general practice in China Medical University. 95.5% of students thought it was necessary to study general practice in the college. 0-10 was used to score the degree of how they want to choose general practice as a career, the median was 0 before they had the class and the median was 5 after the class (Wilcoxon rank test, P<0.01). But there were still some places need to be improved in terms of curriculum design, teaching methods and community practice. The General Practice teaching need to be innovated and the teaching mode should be different from the traditional one.

Objective To investigate the experimental condition of separate purification and directed differentiation potency of pancreatic stem cells of adult rats in vitro. Methods The pancreas of adult rats were digested by collagenase V in situ perfusion. Filtration was performed by 100 mesh filter. Ficoll 400 density gradient centrifugation was used to separate pancreatic stem cells and stem cells were for cultivation. Epidermal growth factor (EGF), fibroblast growth factor (bFGF) was added to medium for in vitro cultivation.Immunofluorescence staining method was used to detect the expression of CK19, Pdx-1, nestin, insulin and glucagon, and the positive cell rate was calculated. The differentiated cell was evaluated by dithizone (DTZ)staining; insulin excretion function was determined by optical enzyme labeled ELISA staining. Results The pancreatic stem cell obtained from the study could be cultivated in vitro for 8 generations. The expression of CK19, Pdx-1 and nestin of the tem cells were all positive, and the rate of positive cells were (88.6 ± 6.2)% ,(84.6 ±8.6)% and (81.3 ±7.5)%. The differentiated cell was in brownish red color by DTZ dye. After high concentration sugar stimulation, the expression of insulin secretion was increased in the supernatant.Conclusions This method can harvest highly purified, and large amount of pancreatic duct stem cell.Artificial induction may result directed differentiate for islet-like clusters and have insulin secrete function.

Adult ; Dimethylformamide ; adverse effects ; Glutathione ; blood ; Humans ; Male ; Methanol ; adverse effects ; Nitric Oxide ; blood ; Occupational Exposure ; Oxygen ; metabolism ; Superoxide Dismutase ; blood ; Surveys and Questionnaires ; Toluene ; adverse effects

Humans ; Infant, Newborn ; Male ; Protein C ; metabolism ; Protein C Deficiency

Adult ; Chromium Alloys ; adverse effects ; Crowns ; adverse effects ; Female ; Foreign Bodies ; etiology ; Foreign-Body Reaction ; Gingiva ; surgery ; Gingival Hyperplasia ; etiology ; surgery ; Gingivectomy ; Humans ; Metal Ceramic Alloys ; adverse effects